2.Protective Effect of Hypothermia in Delayed Astrocyte Death after Transient Histotoxic Hypoxia in Vitro.
Myung Hee KIM ; Baek Hyo SHIN ; Sang Eun LEE
Korean Journal of Anesthesiology 1997;33(3):422-431
BACKGROUND: It is well known that neuronal degeneration can occur after a brief deprivation of energy source. To investigate whether glial astrocyte can induce a phenomenon of delayed cell death after transient energy loss and to see how different are the effects of nifedipine, lidocaine, carnosine and hypothermia on delayed toxicity in astrocyte. METHODS: Human astrocytoma cells (U1242MG) were used in this study. To assess the astrocyte survival during post-ischemic period after transient histotoxic hypoxia, 3-[4,5-dimethylthiazol-2yl]-2,5, diphenyl tetrazolium bromide (MTT) test was used. Compared to MTT test, tryphan blue test was also used to demonstrate membrane damage of affected cells. Studies on intracellular calcium dynamics during ischemic and post-ischemic period were carried out with fluo-3 and flow cytometry system. RESULTS: The percentage survival of astrocyte during post-ischemic period was decreasing with time. Calcium channel blocker nifedipine, sodium and calcium channel blocker lidocaine and free radical scavenger carnosine could not prevent post-ischemic cell damage. But, hypothermia was only an effective method in ameliorating post-ischemic cell death. Intracellular calcium increase during ischemia and post-ischemia was dependent on extracellular calcium influx. CONCLUSIONS: Only hypothermia was effective in reducing astrocyte death during post-ischemia after transient energy depletion. Intracellular calcium alterations during post-ischemia was from extracellular space.
Anoxia*
;
Astrocytes*
;
Astrocytoma
;
Calcium
;
Calcium Channels
;
Carnosine
;
Cell Death
;
Extracellular Space
;
Flow Cytometry
;
Humans
;
Hypothermia*
;
Ischemia
;
Lidocaine
;
Membranes
;
Neurons
;
Nifedipine
;
Sodium
3.A clinical study on the psychological tests and assessment ofpostsurgical satisfaction after orthognathic surgery in the patients with facial deformity.
Kuk Yeop LEE ; Woo Geong JIN ; Hyo Jeun SHIN
Journal of the Korean Association of Oral and Maxillofacial Surgeons 1992;18(4):15-26
No abstract available.
Congenital Abnormalities*
;
Humans
;
Orthognathic Surgery*
;
Psychological Tests*
5.Bilateral Absence of the Vas Deference.
Hyo Shin CHANG ; Jae Heung CHO ; Eung Soo LEE
Korean Journal of Urology 1969;10(4):183-184
A case of bilateral absence of vas deference with sterility is reported. By new 15O cases of congenital absence of the vas deference, rather a rare congenital anomaly, were reported.
Infertility
6.Positional Changes of Reattachment Site after Superior Rectus Recession in Rabbit.
Jung Yoon KWON ; Eu Hyo LEE ; Jae Pil SHIN ; Hyo Churl SONG
Journal of the Korean Ophthalmological Society 1999;40(12):3474-3481
In determining the effects of strabismus surgery, it is very important to know where the reattachment site of an extraocular muscle following recession procedure is located. Undercorrections or overcorrections after muscle surgery have been thought to be due to a postoperative positional changes of reattachment site along the surface of the globe. The author performed this experimental study to evaluate the amounts of changes of reattachment site after recession procedure in rabbit with 4 different methods of scleral fixation;direct suture with 6-0 vicryl, hang-back suture, application with Tisseel and Histoacryl. Superior rectus recession was performed in all 40 rabbit eyes, 10 eyes in each group. The distance from limbus to proximal end of recessed superior rectus muscle was measured on day 1, 2, 3, 5 and 7 postoperatively. Further measurements were followed at 2, 3, 4 and 8 weeks after recession procedure. The muscles were found an average of 0.4mm posterior to the intended position in direct suture group and 0.5 mmin hang-back suture group at one week postoperatively. But in the other two groups in which tissue adhesive agents, Tisseel and Histoacryl were used, the amount of displacement of the reattachment site were minimal of within 0.1 mm. From this experimental study, it is suggested that the positional changes of the reattachment site after recession procedure may influence the surgical corrective effects for strabismus.
Enbucrilate
;
Fibrin Tissue Adhesive
;
Muscles
;
Polyglactin 910
;
Strabismus
;
Sutures
;
Tissue Adhesives
7.CT and MR findings of mycotic infection of the paranasal sinus: differentiation from sinonasal neoplasm.
Kil Woo LEE ; Hyo Keun LIM ; Gwy Suk SEO ; Suk Soo BAE ; Shin Hyung LEE
Journal of the Korean Radiological Society 1992;28(2):176-181
When a soft tissue mass in the bony wall of the paranasal sinus is present, it is difficult to make a distinction between tumor and inflammatory mass on CT. Fungal sinusitis may have soft tissue attenuation on the bony wall of the sinus, bony sclerosis, focal bony destruction, and calcific area on CT. This is a report of four proven cases of fungal sinusitis, asperogillosis in 3 cases and mucormycosis in 1 case, All 4 patients had CT and one patient had MRI, On CT, bony sclerosis and destruction were well visualized in all cases. On MRI, mycetoma in the maxillary sinus was hypointense on T1 weighted images and more hypointense on T2 weighted images. Although CT appears to be the best modality for initial examination of the patient with sinusitis, the differentiation of fungal sinusitis from tumor mass or other entity may be better accomplished with MRI.
Humans
;
Magnetic Resonance Imaging
;
Maxillary Sinus
;
Mucormycosis
;
Mycetoma
;
Sclerosis
;
Sinusitis
8.A Case of Female Pseudohermaphroditism With Congenital Adrenal Hyperplasia.
Hyo Jung KIM ; Hye Kyung LEE ; Hyang Sook KIM ; Soon Jai LEE ; Sung Woo SHIN
Journal of the Korean Pediatric Society 1983;26(6):589-592
No abstract available.
46, XX Disorders of Sex Development*
;
Adrenal Hyperplasia, Congenital*
;
Female*
;
Humans
9.Pulsating Magnetic Field Effects on in vitro Culture of Human Osteogenic Sarcoma Cell Lines.
Hyo Sook SHIN ; Jin Young LEE ; Suk Keun LEE ; Sang Chul PARK ; Je G CHI
Korean Journal of Pathology 2000;34(3):169-180
In order to elucidate the biological effects of pulsating magnetic field in in vitro culture system we designed a pulsating magnetic apparatus using 120 Hertz, 24 Volt direct current. It can generate 63~225 Gauss in the experimental area of 90 mm petri dish, and has little thermal effect on the culture media in 37.5oC, 5% CO2. Human osteogenic sarcoma (HOS) cells were cultured in the pulsating magnetic field and the nuclear changes of cultured cells were observed routinely by hematoxylin staining, and apoptotic change was detected by ApopTag staining using both peroxidase and fluorescein labelings. Compared to the control group which formed well organized whorling pattern of HOS cell line in 3 days culture, the HOS cells cultured in the pulsating magnetic field for 12 hours or 24 hours grew irregularly and showed increased number of apoptotic cells. When the flow of pulsating magnetic field was interrupted by insertion of strong permanent magnetic bar (1000 Gauss, 5530 mm) beneath the petri dish during in vitro culture, the area of sparse pulsating magnetic field showed active proliferation and aggregation of HOS cells even in 24 hour exposure group. These data suggest that the pulsating magnetic field may play a role in inducing growth retardation and apoptosis of HOS cells. Furthermore, the hazardous effects of pulsating magnetic field can be lessened or nullified by the interruption of pulsating magnetic field with a strong permanent magnetic bar.
Apoptosis
;
Cell Line*
;
Cells, Cultured
;
Culture Media
;
Fluorescein
;
Hematoxylin
;
Humans*
;
Magnetic Fields*
;
Osteosarcoma*
;
Peroxidase