Cholera enterotoxin (CT) is a major virulence determinant of Vibrio cholerae 01. CI' is known to be the major virulence factor of Vibrio cholerae 01 and in accordance with the recent report showing which V. cholerae non-01 has ctx gene, we performed the molecular genetic study for the detection of ctx gene related to the production of CT at the subject Vibrio spp. except for V. cholerae non-01 and V. cholerae non-01 stock cultured in the laboratory of microbiology, College of Medicine, Pusan National University and the Vibrio spp. isolated from the marine products of Pusan General Fish Market and the sea water, and then its results are as follows: 1. PCR for the detection of ctx gene at the subject of V. cholerae 01:61H-151 having the ctx gene of which the denaturation is 1 rninute at 95'C, annealing to 1min, 30 sec at 60'C, the extension to be 1min. 30 sec at 72'C and 30 or 40 cycles. ctx gene was detected from 4 strains of V. cholera non-01 derived from the environment isolates. 2. Adjusting the quantity of chromosomal DNA used as template DNA to be from 0.1 pg to 1 ng, in order to know the PCR conditions for the effective search of ctx gene, and the detection limit of the system was 10 pg of chromosomal DNA. 3. The broth culture was used for template DNA, ctx gene of 302 bp was detected from 4 V. cholerae non-01, as in the case of chromosomal DNA, and the cell number was possible to be detected to 3 * 10.4. We attempted the confirmation of ctx gene through Southern blot hybridization, labeling with P and then it was confirmed only from 4 V. cholerae non-01 as like PCR results. 5. As the result of the sensitivity of PCR and Southern blot hybridization, it was shown to be possible which 10 pg was detected in case of chromosomal DNA and in case of cultured broth, the cell number was detected until 10 at PCR and Southern blot hybridization, and thus it was examed which its sensitivity was same.
Blotting, Southern ; Busan ; Cell Count ; Cholera Toxin* ; Cholera* ; DNA* ; Enterotoxins ; Limit of Detection ; Molecular Biology ; Operon* ; Polymerase Chain Reaction* ; Seawater ; Vibrio cholerae* ; Vibrio* ; Virulence

OBJECTIVES: This study is to offer basic data to understand the relationships between mental health, level of depression, and internet addiction of high school students in farming communities for developing a mental health management program for adolescents. METHODS: The survey was carried out on a convenience sample of 299 high school students in farming communities during May of 2008. Data analysis procedure included chi-square -test, t-test, Pearson correlation among Adolescent Mental Health & Problem-behavior Screening Questionnaire (AMPQ), Children's Depression Inventory (CDI), and Scales of Internet addiction (K-scales). RESULT: First, the level of mental health according to the AMPQ for subjects from this study showed problematic behavior was lower when compared to other researches. There were statistically significant differences according to the school type for externalization problems and overall problematic behavior. Based on gender, it was even more problematic for male students in regards to externalization problems. Secondly, the level of depression was relatively low : 5.1% for potential risk and 0.3% for high risk. Thirdly, a total of 96.9% were considered normal for Internet addition levels. 1.7% for potential risk, 1.4% for high risk; however, there was no statistically significant difference between each variable. Fourthly, there was a strong relationship between subjects AMPQ, level of depression and Internet addiction. As depression worsens, Internet addiction also becomes stronger. CONCLUSION: There is a need for awareness of the mental health of adolescents and precautionary measures, the development of a program for early treatment, adequate management, and decisions on the direction of treatment.
Adolescent ; Depression ; Humans ; Internet ; Male ; Mass Screening ; Mental Health ; Rural Population ; Statistics as Topic ; Weights and Measures ; Surveys and Questionnaires

No abstract available.
Escherichia coli* ; Escherichia*

No abstract available.
Escherichia coli* ; Escherichia*

BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) are becoming increasingly responsible for the outbreaks of nosocomial infections around the world. Because MRSA are often resistant to antimicrobial agents and because of the current necessity to use non-beta-lactam antimicrobial therapy, infections with these organisms are difficult to treat. The purpose of this study was to explain how the genetic background contributes to the phenotypic expression of MRSA and to enable immediate and proper identification of MRSA in nosocomial infections. METHODS: A total of 240 staphylococci strains were tested for epidemiological research. The molecular genetic methods, such as dot blot hybridization, polymerase chain reaction, and southern blot hybridization, were compared in terms of the immediate and proper identification of the pathogens. The arrangement of dru sequence, the repeat unit of genes, was clarified particularly through mecA probe to compare and distinguish the isolated MRSA strains. RESULTS: By the dot blot hybridization, the mecA gene was detected in 120 MRSA strains and in 8 of 50 methicillin-suscpetible S. aureus (MSSA) in Group II (resistance to methicillin is induced in vitro). Among isolates included in Group I (resistance to methicillin is not induced in vitro) mecA genes were not detected. By PCR, the amplified DNA band of 533 bp was confirmed in 120 MRSA but not in MSSA. By the southern blot hybridization, the signal of the amplified electrophoresis band in PCR was similarly observed. The amplified band of mec related hypervariable region was observed in all methicilin resistant (MR) staphylococci but not in MSSA. The size of PCR products was between 194 bp and 1,353 bp. mec-related HVR was classified into seven genotypes. The nucleotide sequence of genotype E was similar to that of mec related HVR and eight units were directly repeated. CONCLUSION: Both methods allowed rapid detection of mecA gene. Further differentiation of MRSA and other staphylococci strains was possible by PCR detection of polymorphism of HVR sequence which would be useful in tracing back the source of resistant clones.
Anti-Infective Agents ; Base Sequence ; Blotting, Southern ; Clone Cells ; Cross Infection ; Disease Outbreaks ; DNA* ; Electrophoresis ; Genotype ; Methicillin ; Methicillin Resistance* ; Methicillin-Resistant Staphylococcus aureus ; Molecular Biology* ; Polymerase Chain Reaction*

OBJECTIVES: The purpose of this study was to investigate changes in public recognition of parabens on Twitter and the research status of parabens related to toothpaste. METHODS: Tweet information between 2010 and October 2016 was collected by an automatic web crawler and examined according to tweet frequency, key words (2012-October 2016), and issue tweet detection analyses to reveal changes in public recognition of parabens on Twitter. To investigate the research status of parabens related to toothpaste, queries such as “paraben,”“paraben and toxicity,”“paraben and (toothpastes or dentifrices),” and “paraben and (toothpastes or dentifrices) and toxicity” were used. RESULTS: The number of tweets concerning parabens sharply increased when parabens in toothpaste emerged as a social issue (October 2014), and decreased from 2015 onward. However, toothpaste and its related terms were continuously included in the core key words extracted from tweets from 2015. They were not included in key words before 2014, indicating that the emergence of parabens in toothpaste as a social issue plays an important role in public recognition of parabens in toothpaste. The issue tweet analysis also confirmed the change in public recognition of parabens in toothpaste. Despite the expansion of public recognition of parabens in toothpaste, there are only seven research articles on the topic in PubMed. CONCLUSIONS: The general public clearly recognized parabens in toothpaste after emergence of parabens in toothpaste as a social issue. Nevertheless, the scientific information on parabens in toothpaste is very limited, suggesting that the efforts of dental scientists are required to expand scientific knowledge related to parabens in oral hygiene measures.
Oral Hygiene ; Parabens* ; Toothpastes*

A total of 100 Vibrio spp. strains were examined for production of various extracellular enzyme and for plasmid content plasmid were subjected to digestion with restriction enzymes. Most of them produced extracellular enzyme more than one, especially V. parahaemolyticus and V. cholerae non-01 strains were showed production of various extracellular enzymes. About the 55% Vibrio spp. have the plasmid more than one, but a lot of Vibrio spp. (about 45%) did not possess any plasmid. Most of these plasmid various derivatives ranged from 2.4 kb-23 kb, especially two strains of V. mimicus and one strain of V. furnissii carried one high-molecular weight plasmid (molecular weight ranging between 70 kb-100 kb). Results of restriction analysis for plasmid of this three strains were by no means the rule. For detection of tdh and ctx gene, the virulence factor involved in the pathogenesis, we carried out the TDH, CT assay, PCR amplification, and hybridization. A total 11 strains were produced TDH, involved in 4 strains of V. parahaemolyticus and 1 strain of V. cholerae non-01 from clinical isolates and 6 strains of environmental isolates. Nine strains of 11 strains, involved in 4 strains of V. parahaemolyticus and 1 strain of V. cholerae non-01 from clinical isolates and 4 strains of V. parahaemolyticus from environmental isolates, could be successfully amplified in 400 bp by PCR, no amplification products were obtained from TDH-negative strains. The PCR results were consistent with DNA hybridization. In the experiments of ctx gene detection, in all, 3 strains of V. cholerae non-01 from clinical isolate and 1 strains of V. cholerae non-01 from environmental isolate were observed CT- positive. These CT-producing strains amplified in 302 bp by PCR for the detection of ctx gene. All CT-producing strains hybridized with digoxigenin-labeled DNA probe, while CT-negative strains did not hybridize. Also hybridization tests results for detection of ctx gene consistent with PCR.
Cholera ; Digestion ; DNA ; Plasmids ; Polymerase Chain Reaction ; Vibrio* ; Virulence Factors* ; Virulence*

No abstract available.
Animals ; Interleukin-2 ; Mice*

PURPOSE: This study was to analyze the effect size of complementary and alternative intervention studies in reference to dysmenorrhea and menstrual distress. METHODS: In order to conduct a meta-analysis, a total of 393 studies were retrieved from the database. Twenty-eight studies that were published from March 2001 to February 2011 were selected. RESULTS: Intervention studies included seven studies on aromatherapy, five on auriculotherapy, three on each Koryo-Sooji-Chim and moxibustion, two on each heat therapy and magnetic therapy and six on other therapy. The effect size of the intervention studies on dysmenorrhea and menstrual distress was greater than 0.48 for Koryo-Sooji-Chim, moxibustion, aromatherapy, auriculotherapy and other therapy. CONCLUSION: This study suggests that drug free therapy can reduce the levels of menstrual distress, despite the small number of intervention studies and randomized controlled trials.
Aromatherapy ; Auriculotherapy ; Dysmenorrhea ; Female ; Hot Temperature ; Clinical Trial ; Magnetics ; Magnets ; Menstruation ; Moxibustion

BACKGROUND: This study aimed to investigate bradycardia as an adverse effect after administration of dexmedetomidine during 33degrees C target temperature management. METHODS: A retrospective study was conducted on patients who underwent 33degrees C target temperature management in the emergency department during a 49-month study period. We collected data including age, sex, weight, diagnosis, bradycardia occurrence, target temperature management duration, sedative drug, and several clinical and laboratory results. We conducted logistic regression for an analysis of factors associated with bradycardia. RESULTS: A total of 68 patients were selected. Among them, 39 (57.4%) showed bradycardia, and 56 (82.4%) were treated with dexmedetomidine. The odds ratio for bradycardia in the carbon monoxide poisoning group compared to the cardiac arrest group and in patients with higher body weight were 7.448 (95% confidence interval [CI] 1.834-30.244, p = 0.005) and 1.058 (95% CI 1.002-1.123, p = 0.044), respectively. In the bradycardia with dexmedetomidine group, the infusion rate of dexmedetomidine was 0.41 +/- 0.15 microg/kg/h. Decisions of charged doctor's were 1) slowing infusion rate and 2) stopping infusion or administering atropine for bradycardia. No cases required cardiac pacing or worsened to asystole. CONCLUSIONS: Despite the frequent occurrence of bradycardia after administration of dexmedetomidine during 33degrees C target temperature management, bradycardia was completely recovered after reducing infusion rate or stopping infusion. However, reducing the infusion rate of dexmedetomidine lower than the standard maintenance dose could be necessary to prevent bradycardia from developing in patients with higher body weight or carbon monoxide poisoning during 33degrees C targeted temperature management.
Atropine ; Body Weight ; Bradycardia* ; Carbon Monoxide Poisoning ; Dexmedetomidine* ; Diagnosis ; Emergency Service, Hospital ; Heart Arrest ; Humans ; Hypothermia, Induced ; Logistic Models ; Odds Ratio ; Retrospective Studies