No abstract available.
Keratosis, Seborrheic ; Skin Diseases ; Skin Diseases, Papulosquamous

BACKGROUND: It has been known that blood group antibodies are not produced in the neonatal period and that if the antibodies exist, they are probably maternal in origin which had crossed the placenta. There have been several studies conducted abroad on when these antibodies are formed but none has been done in Korea. This study was carried out to determine the ABO blood type and blood group antibodies in children from neonates up to 5 year old. We hoped to determine when and in what pattern blood group antibodies were produced. METHODS: We selected 337 children from neonates up to 5 year old who were admitted to Hanyang university Hospital in Seoul or Kuri from 1994 to 1996. Cell typing was done immediately by the slide method. The anti-A and anti-B used for cell typing were supplied by Immucor (Norcrosis, Ga) . Sera were stored at -70 degrees C until they were tested for ABO blood group antibodies by the standard saline test tube method. When uncertain results were obtained, a drop of the mixture was placed on a slide and observed under a microscope. RESULTS: ABO blood group antibodies were detected in 9 of 50 (18%) infants less than 1 week old and in 10 of 51 (20%) infants between 1 week and 3 months of age. The pattern of ABO blood group antibody production was similar to that of the fetal period up to 3 months after birth, after which antibody production increased rapidly to reach approximately 80% at 6 months of age, There was no difference in ABO antibody production between boys and girls. The antibody formation pattern of group A and group B infants less than 6 months of age showed anti-A to be 35% and anti-B to be 20%. In group O infants of the same age, anti-A was positive In 42% and antral-B In 33%. However, after 6 months of age, there was no difference in antibody production among groups A, B, or O. CONCLUSIONS: Antibodies directed toward ABO antigens were detected in 19 out of 101 (19%) infants less than 3 months old. We therefore believe it is necessary to Perform serologic typing as well as cell typing in these Infants. Furthermore, the emergency transfusion of type A or B blood to a type O infant under the impression that anti-A and anti-B do not exist should be forbidden.
Antibodies* ; Antibody Formation ; Child* ; Child, Preschool ; Emergencies ; Female ; Hope ; Humans ; Infant ; Infant, Newborn ; Korea ; Parturition ; Placenta ; Seoul

The immune response to M. tuberculosis engages T-cell medicated reactions which determine the degree of resistance and also the clinical pattern of disease. Although antibodies produced by the infected host have yet no proven protection, they are appilicable for alternative diagnostic tests in tuberculosis. Preparation of purified antigens from Mycobacteria with specific antigenic determinants would improve serological diagnosis in tuberculosis. The antigen was prepared by extraction of M. tuberculosis with perchloric acid which was found to be major bands of 71, 42, 38 and 10 kD by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Thirteen sera from patients with active tuberculosis showed positive reactions to TB-P by ELISA, but the reaction to perchloric acid extract antigen is weaker than to PPD. The IgG subclass profiles to TB-P were IgG1, IgG2 and IgG4. By means of western blotting, the antibodies in the tuberculous patients showed the reaction with antigens of lower molecular weight such as 14, 12, 11 and 10 kD. These results suggested that the perchloric acid soluble antigen of M. tuberculosis might be more related with cell-mediated immune reactions rather than humoral immune reactions.
Antibodies ; Blotting, Western ; Diagnosis ; Diagnostic Tests, Routine ; Electrophoresis ; Enzyme-Linked Immunosorbent Assay ; Epitopes ; Humans ; Immunoglobulin G ; Molecular Weight ; Sodium ; T-Lymphocytes ; Tuberculosis*

The presence of anti-dsDNA is included in diagnostic criteria for systemic lupus erythematosus (SLE) according to the American College of Rheumatology (ACR). It has been the most useful factor in the diagnosis, prognosis, and therapeutic monitoring of patients with SLE. A number of methods are available but radioimmunoassay (RIA) has been regarded as standard method. A shift from RIAs to nonisotopic assay has been observed with other tests. Still, RIA assays are standard methods for anti-nDNA antibodies. A comparative study of the Crithidia luciliae immunofluorescence (CLIF) assay and an RIA was made. METHOD: Sera from 144 patients were tested by an indirect immunofluorescent antibody technique employing Crithidia luciliae and IT-1 cell lines as a substrate and radioimmunoassay was based on the Farr technique. RESULTS: 1. Thirty-nine of 122 sera with positive antinuclear antibody (ANA) tests had the possibility of positive anti-nDNA antibodies. 2. The RIA was positive in 54 sera, and 37 of these showed a discrepancy between the RIA and the ANA pattern (false positive rate 25.7%). 3. The CLIF was positive in 15 sera, and 5 of these showed a discrepancy between CLIF and the ANA pattern (false positive rate 3.5%). 4. Only CLIF was positive in 2 sera of which one showed a discrepancy between CLIF and the ANA pattern. 5. Only RIA was positive in 41 sera, and 33 of these showed a discrepancy between RIA amp; the ANA pattern. CONCLUSION: The immunofluorescence assay using Crithidia luciliae is a valid method to detect anti-dsDNA antibodies and has a much lower false positive rate compared with RIA. The simple and inexpensive CLIF test could either replace the RIA in clinical laboratories or be used in conjunction with the ANA pattern as a confirmatory test for antibodies to nDNA.
Antibodies* ; Antibodies, Antinuclear ; Cell Line* ; Crithidia* ; Diagnosis ; Fluorescent Antibody Technique ; Humans ; Lupus Erythematosus, Systemic ; Prognosis ; Radioimmunoassay ; Rheumatology

No abstract available.

No abstract available.
Adult* ; Humans

No abstract available.
Adult* ; Humans

No abstract available.
Melanoma*

No abstract available.
Gestational Age* ; Intracranial Hemorrhages*

BACKGROUND: Recently, the incidence of hepatitis A virus (HAV) infection has been increasing in Korea, and an outbreak among healthcare workers (HCWs) has also been reported. This study aims to evaluate the seroepidemiology of hepatitis A among HCWs at an 820-bed teaching hospital in Korea, thereby facilitating HAV vaccination for these HCWs. METHODS: In June 2009, we assessed the total immunoglobulins against the HAV in the serum samples of 907 HCWs by using VIDAS Anti-HAV Total (bioMerieux, France). RESULTS: Seropositivity for HAV significantly increased with age (P<0.001): 0% for < or =25 years, 9.4% for 26-30 years, 39.5% for 31-35 years, 66.7% for 36-40 years, 85.7% for 41-45 years, 96.0% for 46-50 years, 98.3% for 51-55 years, 95.8% for 56-60 years, and 100% for > or =61 years. CONCLUSION: Since HCWs aged 20-40 years show low seropositivity for HAV vaccination against hepatitis A should be considered in all these HCWs.
Aged ; Delivery of Health Care ; Hepatitis ; Hepatitis A ; Hepatitis A Antibodies ; Hepatitis A virus ; Hospitals, Teaching ; Humans ; Immunoglobulins ; Incidence ; Korea ; Seroepidemiologic Studies ; Vaccination