No abstract available.
Alcoholics* ; Humans ; Liver Diseases* ; Liver* ; Pancreatitis, Alcoholic*

No abstract available.

No abstract available.
Female ; Humans

No abstract available.
Humans ; Liver Function Tests* ; Liver*

Blue nevi commonly occur on the skin of head, neck, and arms, and in occasional instances they have been observed in the mucosa of oral cavity, vagina, hard palate, and even breast, and in very rarity observed in the uterine cervix. We have experienced a case of blue nevi on the uterine cervix of a 45 year old famale who was operated under the diagnosis of uterine myoma. In gross findings, besides two well circumscribed uterine myomas measuring 3.5 cm and 0.6 cm in diameter in the anterior myometrium, multiple pin-point sized grayish blue pigments measuring 2-3 mm in diameter aggregated in the submucosa of the uterine cervix. Microscopically the blue nevi showed greatly elongated, slender often slightly wavy melanocytes with long, occasionally branching dendritic processes lie grouped in irregular bundles in the submucosa of the uterine endocervix. The pigments showed positive response to the Fontana-Masson stain in the cytoplasm and the extracellular area.
Female ; Humans

No abstract available.
Cell Culture Techniques* ; Chorionic Gonadotropin* ; Epidermal Growth Factor* ; Humans* ; Trophoblasts*

No abstract available.
Hepatitis* ; Polymerase Chain Reaction*

Human papillomavirus (HPV)infection are now generally accepted as the most important factor for development of uterine cervical cancer and its precursor lesions. With increasing evidences that the HPV E7 encodes for oncoproteins critical for viral replication, host cell immortalization and transformation. Based on the previous reports that the high risk HPV type 16 DNA is frequently detected in specimens from Korean women with cervical cancer and that there is the sequence variation and geographical dependence of HPV 16 E7 gene in preinvasive and invasive cervical lesions, it is crucial to determine the prevalence of HPV 16 variants in uterine cervical lesions of Korean women. This study was performed to identify sequence variations of HPV 16 E7 gene and an association between HPV 16 E7 variants and uterine cervical cancer. The author has determined nucleotide sequences of the E7 gene of HPV 16 isolated from uterine cervical tissues in Korean women. HPV 16 DNAs were detected by the nested PCR in 112 (24.5%) of a total of 457 samples. By direct sequencing of PCR-HPV 16 E7 positive cases, 79 samples (70.5%) showed variant sequences, while the prototype sequence was found in only 33 samples (29.5%). Twenty-three cases (57.5%) of 40 normal cervical samples showed sequence variation. Forty-eight (77.4%) of 62 cervical cancer cases showed sequence diversity from prototype HPV 16 E7 gene. There were four types of sequence variations. A single nucleotide change at position 647 (A-->G) was found in 52 cases (65.8%) of 79 HPV 16 E7 variants. Predicted amino acid change (Asn -->Ser) was found in the HPV 16 E7 oncoproteins at amino acid position at 29. And this KE7-1 variant was commonly detected in the uterine cervical cancer compared to the normal cervix. The second most common variant, detected in 16 cases (20.3%), had three silent mutations at nucleotide positions 732 (T-->C), 789 (T-->C) and 795 (T-->G). The third variant had a single nucleotide change at position 666 (G-->A), and the fourth had a change at position 796 (T-->C). Furthermore, PCR-SSCP clearly showed distinct bands compatible with HPV 16 E7 variants as with the direct-sequencing method. PCR-SSCP was also an effective and reliable tool in detecting HPV 16 E7 variants. This study showed that there were four variant types of HPV 16 E7 in uterine cervical tissues and KE7-1 with corresponding amino acid change was the most commonly detected type in E7 variants of HPV 16 isolated from uterine cervical cancer in Korean women.
Base Sequence ; Cervix Uteri ; DNA ; Female ; Human papillomavirus 16 ; Humans* ; Oncogene Proteins ; Polymerase Chain Reaction ; Polymorphism, Genetic* ; Prevalence ; Uterine Cervical Neoplasms*

Carcinoma of the uterine cervix has been considered to be a sexually transmitted disease(STD) and at present time, particullary human papillomavirus (HPV) is considered as the most likely infectious causative agents of uterine cervical cancer. But less is known about the sexual transmission of HPV and the status of HPV infection of male partner. Therefore, screenng of couples for HPV is very important for understanding HPV infection as a sexually transmitted disease and prevention of cervical carcinoma. The polymerase chain reaction(PCR) was employed to detect HPV 16 and 18 in cytological samples from the uterine cervix of the patients with cervical carcinoma(4 CIS and 34 invasive cervical carcinoma) and from urethral metatus and glans sulcus of their male consorts. The results are as follows; 1. HPV 16 or 18 were detected in 31(81.6%) of 38 patients with cervical cancer(HPV 16; 78.9%(30/38), HPV 1S; 28.9%(11/38), HPV 16 and 18; 26.3%(10/38)), 2. HPV 16 was detected in 27(90,0%) of 30 males whose wives were positive for HPV 16. But HPV 18 was detected in only 3(27.3%) of 11 male consorts whose wives were positive for HPV 18. And HPV 1S was detected in all male consorts whose wives were positive for HPV 16. In addition, HPV 16 or 18 were positive in 3 of 7(42.9%) male consorts whose wives were negative for HPV 16 and 18. Conclusively, these results suggest that HPV might be transmitted by sexual contacts in heterosexual couples.
Cervix Uteri ; Family Characteristics* ; Female ; Heterosexuality* ; Human papillomavirus 16 ; Human papillomavirus 18 ; Humans* ; Male ; Polymerase Chain Reaction ; Sexually Transmitted Diseases ; Spouses ; Uterine Cervical Neoplasms

No abstract available.
Epidermal Growth Factor* ; Receptor, Epidermal Growth Factor* ; Uterine Cervical Neoplasms*