OBJECTIVES: Cadherin/catenin adhesion complex is fundamentally involved in epithelial cancer invasion and metastasis. E-cadherin and EGFR colocalize on the basolateral membrane of epithelial cell and EGF down-regulate E-cadherin expression. In the invasion and metastasis of cancer, E-cadherin expression is decreased and growth factors receptor is overexpressed. The present study was aimed to find the role of E-cadherin, beta-and gamma-catenin, growth factors and its receptors in cervical cancer cell lines. METHODS: The cervical cancer cell cultures were treated with different time duration of EGF 30 ng/ml and TGF-a 10 ng/ml(0, 10 min, 20 min, 30 min, 1 hr, 2 hr, 4 hr, 8 hr, 24 hr). The change in cancer cell morphology and the changes in E-cadherin, beta- and gamma-catenin, EGFR and activated EGFR expression were studied with a western blot analysis and an immunoprecipitation. RESULTS: Through a western blot analysis, E-cadherin 120 kDa band and EGFR 170 kDa band were expressed in CaSki, HT-3 and ME-180 cell line, which showed epithelial contact growth. 1n these 3 cell lines, expression of E-cadherin did not decrease with time dependent manner. after the treatment of EGF and TGF- alpha. The expression of EGFR decreased and activated EGFR expression increased in 30 minutes to 1 hour but decreased subsequently. When the cells treated with EGF, there were no change in beta-and gamma-catenin expression with there dependent manner. The tyrosine phosphorylation of beta-and gamma-catenin increased in 30 minutes to 1 hour but decreased subsequently with activated EGFR. CONCLUSION: This study showed that an activated EGFR which has involved with tyrosine phosphorylation of beta- and gamma-catenin influenced by growth factors rather than expression of E-cadherin, has a role in the invasion and metastasis of the cervical cancer.
Blotting, Western ; Cadherins* ; Cell Culture Techniques ; Cell Line* ; Epidermal Growth Factor* ; Epithelial Cells ; gamma Catenin* ; Immunoprecipitation ; Intercellular Signaling Peptides and Proteins ; Membranes ; Neoplasm Metastasis ; Phosphorylation* ; Transforming Growth Factor alpha* ; Tyrosine* ; Uterine Cervical Neoplasms*

The purpose of this study were to find out the practical way to enlarged child-raising knowledge and to enhance their satisfaction with out-patient care by evaluating how effectively the education is done by nurses for mothers with ill-child and how their satisfaction with out-patient care changed. This study was designed as a Nonequivalent Control Group study. The subjects studied were consisted of the experimental and control group. Each consisted of 50 mothers with ill-child in pediatric department at one university hospital in Seoul. The period of this study is from May 20, 1996 to June 28, 1996. The first data were collected fro both of experimental and control groups in which mothers with ill-child come to the hospital for the first time. After this being done, the experimental group had been educated by the planned program and then the second data were collected from them. On the contrary, as for the control group, there had been no education and the second data were collected on the same method. The data analysis was done by SPSS program. The results of this study are as follow, 1. The child-raising knowledge level of mothers with education was higher than that of with no education.(t=18.84, df=49, p=0.000) 2. The satisfaction with out-patient care level of mothers with education was higher than that of no education.(t=10.51, df=49, p=0.000) Based on these results, I suggest as follow, 1. The research on the patients and their family should be made not only in pediatric department, but in every out-patient department. 2. For more effective education, it is required for all out-patient nurses to research the education demand of patients and their family. 3. To increase the effect of education, there must be the consultation room in out-patient department. 4. The meetings with the mothers with ill-child of the same illness have to be established and periodical education must be executed. 5. Audio-visual education programs like video tapes are needed to make use of waiting time for the medical treatment. 6. On-line consulting program are needed.
Education ; Humans ; Mothers* ; Outpatients* ; Seoul ; Statistics as Topic ; Child Health

M llerian anomalies have been infrequently encountered clinical problem for the obstetrician gynecologist, incidence was estimated to occur in 1% to 6% of all women. Recently, the use of vaginal ultrasound, MRI and endoscopic techniques have led to more accurate description and classification of m llerian anomalies. This report of a complete uterine septum with duplication of the cervix and a longitudinal vaginal septum with hemivaginal stricture represents a unique case of septate uterus, which to our knowledge previously has not been reported.
Cervix Uteri* ; Classification ; Constriction, Pathologic* ; Female ; Humans ; Incidence ; Magnetic Resonance Imaging ; Ultrasonography ; Uterus*

No abstract available.
Antioxidants* ; Oxidative Stress*

BACKGROUND: The purpose of study to present the basic data for enhancing the performance level of the dental clinic infection prevention through the actual condition of the implementation for dental treatment infection prevention aimed at dental hygienists in each hospital. METHODS: We carried out a survey targeting 69 dental hygienists in the dental college hospital, 42 in the dentist of the Dentistry in General Hospital, 51 in the dental clinic and 38 in the dentist's office and the medical institutions surveyed, and we used frequency analysis and chi-square analysis by using SPSS (Ver. 18.0). RESULTS: The experiences wounded in the body by sharp instruments or needles contaminated with patients' blood or saliva were on average in 86.5% of the dental hygienists, and people who disinfected the wound with disinfectants after washing their own hands and squeezing blood as the method to fight the wound was 73.4%. The dental clinic was the highest in case of the rate of wearing gloves, and the dental clinic was the highest in the case of the rate of wearing a mask. The use of the liquid soap containing antimicrobial was the highest as 28.0% in the dental college hospital in case of 'the cleanser to be used for washing their own hands'. In the multiple response for dental treatment mechanical equipment management apparatus and the actual condition to be equipped with medicines and the survey in sterilization, Povidone was the highest as 28% in the dental college, and surface disinfectants were being used in some hospitals. CONCLUSION: To prevent the infection in the consultation room, dental hygienists should thoroughly recognize Dental Treatment Infection Prevention Standards, and the research measures or administrative support of the government and the organs concerned is needed a lot.
Dental Clinics ; Dental Hygienists ; Dentistry ; Dentists ; Disinfectants ; Hand ; Hospitals, General ; Humans ; Masks ; Needles ; Povidone ; Saliva ; Soaps ; Sterilization

We report a case of mucinous cystadenoma of uncertain malignant potential and carcinoid of appendix associated with bilateral mucinous cystadenoma of ovary and pseudomyxoma peritonei. The patient was a 46-year-old female. She suffered from dyspnea and lower abdominal palpable masses for several months. Ultrasonogram showed multilocular huge ovarian cysts. Appendectomy, transabdominal hysterectomy, bilateral salphingo-oophorectomy and biopsy of omentum were performed. The bilateral ovaries measured 16 11 cm and 7X5 cm in size, respectively. The both ovaries showed multilocular cysts filled with thick mucus material. The ovarian cysts were covered by a single layer of columnar epithelium with focal proliferation. Mucus materials dissected through the ovarian stroma (pseudomyxoma ovarii). The tip of appendix was dilated and covered by mucus material. The cut surface showed a cyst and a yellowish solid mass proximal to the cyst. Microscopically, the appendiceal cyst was lined by stratified columnar epithelium with moderate cytologic atypia. Mucus material dissected through the wall. In the proximal portion of the appendix, a classic carcinoid with focal tubular form was present in submucosa and muscle layer. The omentum was covered by thick mucus material. Microscopically, the omentum showed mucinous epithelium and mucus material (pseudomyxoma peritonei).
Appendectomy ; Appendix* ; Biopsy ; Carcinoid Tumor* ; Cystadenoma, Mucinous ; Dyspnea ; Epithelium ; Female ; Humans ; Hysterectomy ; Middle Aged ; Mucins* ; Mucus ; Omentum ; Ovarian Cysts ; Ovary* ; Pseudomyxoma Peritonei* ; Ultrasonography

We report a case of mucinous cystadenoma of uncertain malignant potential and carcinoid of appendix associated with bilateral mucinous cystadenoma of ovary and pseudomyxoma peritonei. The patient was a 46-year-old female. She suffered from dyspnea and lower abdominal palpable masses for several months. Ultrasonogram showed multilocular huge ovarian cysts. Appendectomy, transabdominal hysterectomy, bilateral salphingo-oophorectomy and biopsy of omentum were performed. The bilateral ovaries measured 16 11 cm and 7X5 cm in size, respectively. The both ovaries showed multilocular cysts filled with thick mucus material. The ovarian cysts were covered by a single layer of columnar epithelium with focal proliferation. Mucus materials dissected through the ovarian stroma (pseudomyxoma ovarii). The tip of appendix was dilated and covered by mucus material. The cut surface showed a cyst and a yellowish solid mass proximal to the cyst. Microscopically, the appendiceal cyst was lined by stratified columnar epithelium with moderate cytologic atypia. Mucus material dissected through the wall. In the proximal portion of the appendix, a classic carcinoid with focal tubular form was present in submucosa and muscle layer. The omentum was covered by thick mucus material. Microscopically, the omentum showed mucinous epithelium and mucus material (pseudomyxoma peritonei).
Appendectomy ; Appendix* ; Biopsy ; Carcinoid Tumor* ; Cystadenoma, Mucinous ; Dyspnea ; Epithelium ; Female ; Humans ; Hysterectomy ; Middle Aged ; Mucins* ; Mucus ; Omentum ; Ovarian Cysts ; Ovary* ; Pseudomyxoma Peritonei* ; Ultrasonography

Juvenile dermatomyositis is an uncommon autoimmune disease with classic heliotrope discoloration of eyelids, erythematous skin rash of joints and proximal muscle weakness. Quite different from adults, malignancy is rarely accompanied in juvenile dermatomyositis. However vasculitis, muscle atrophy, calcification and gastrointestinal involvement are often observed in juvenile dermatomyositis. A six year old boy was admitted with chief complaints of general weakness and skin rash. Muscle biopsy was performed which was consistent with dermatomyositis. The patient was treated with intravenous immunoglobulin, steroid, methotrexate and physiotherapy. We report a case of juvenile dermatomyositis.
Adult ; Autoimmune Diseases ; Biopsy ; Dermatomyositis* ; Exanthema ; Eyelids ; Humans ; Immunoglobulins ; Joints ; Male ; Methotrexate ; Muscle Weakness ; Muscular Atrophy ; Vasculitis

PURPOSE: Due to the increasing numbers of radical prostatectomies (RP) performed for prostate cancer, a substantial and increasing number of patients suffer from postoperative urinary incontinence and erectile dysfunction (ED). The objective of our study was to see whether an inflatable penile prosthesis implantation could control urinary incontinence for patients with the dual problems of ED and incontinence. MATERIALS AND METHODS: From March 2010 through May 2015, 25 post-RP patients were referred to our clinic with ED or incontinence. The degree of incontinence was classified according to the International Consultation on Incontinence Questionnaire-Short Form. Inflatable penile prostheses were implanted in all 25 patients. RESULTS: For one month after implantation, partial or full inflation was performed progressively to control urine leakage. Of 18 patients, 13 patients were categorized with mild or moderate stress incontinence. All 13 patients obtained control of incontinence with partial inflation (30% to 60%) and all reported satisfactory outcomes. Five out of the 18 patients were categorized with severe total incontinence. Three of the 5 patients could tolerate incontinence with full inflation on and off. Thirteen patients out of the total of 18 (72.2%) had their incontinence controlled by an inflating penile prosthesis. CONCLUSIONS: An inflatable penile prosthesis is highly recommended as an initial procedure, especially in patients with the dual problems of ED and incontinence.
Erectile Dysfunction ; Humans ; Inflation, Economic ; Male ; Penile Implantation ; Penile Prosthesis* ; Prostatectomy ; Prostatic Neoplasms ; Urinary Incontinence*

PURPOSE: The purpose of this study was to compare the premenstrual syndrome (PMS) and the relationships among PMS, perfection, and anxiety in nursing college students and general college students. METHODS: The questionnaire survey was carried out with a convenience sample of 215 college students. The MDQ (Menstrual Distress Questionnaire), APS-R (Almost Perfect Scale-Revised), STAI (State and Trait Anxiety Inventory) were used. The data were collected from June to October, 2011. Data analysis included frequency, chi2-test, t-test, and Pearson correlation. RESULTS: There were significant differences in PMS and perfection but there not in anxiety between nursing college students and general college students. There were significant correlations among PMS, perfection and anxiety in nursing college students, and there were significant correlations among PMS and anxiety in general college students but not significant correlations among PMS and perfection. CONCLUSION: The findings suggest that to provide effective interventions for PMS in college students, it is necessary to design nursing interventions that consider perfection and anxiety.
Anxiety* ; Female ; Humans ; Menstruation ; Nursing ; Premenstrual Syndrome* ; Surveys and Questionnaires ; Statistics as Topic