1.The expression and tyrosine phosphorylation of E-cadherin, beta-, gamma-catenin and EGFR after treatment of EGF and TGF-alpha in Cervical Cancer Cell Lines.
Hye Sung MOON ; Eun Ah CHOI ; Hye Young PARK
Korean Journal of Gynecologic Oncology and Colposcopy 2000;11(1):13-23
OBJECTIVES: Cadherin/catenin adhesion complex is fundamentally involved in epithelial cancer invasion and metastasis. E-cadherin and EGFR colocalize on the basolateral membrane of epithelial cell and EGF down-regulate E-cadherin expression. In the invasion and metastasis of cancer, E-cadherin expression is decreased and growth factors receptor is overexpressed. The present study was aimed to find the role of E-cadherin, beta-and gamma-catenin, growth factors and its receptors in cervical cancer cell lines. METHODS: The cervical cancer cell cultures were treated with different time duration of EGF 30 ng/ml and TGF-a 10 ng/ml(0, 10 min, 20 min, 30 min, 1 hr, 2 hr, 4 hr, 8 hr, 24 hr). The change in cancer cell morphology and the changes in E-cadherin, beta- and gamma-catenin, EGFR and activated EGFR expression were studied with a western blot analysis and an immunoprecipitation. RESULTS: Through a western blot analysis, E-cadherin 120 kDa band and EGFR 170 kDa band were expressed in CaSki, HT-3 and ME-180 cell line, which showed epithelial contact growth. 1n these 3 cell lines, expression of E-cadherin did not decrease with time dependent manner. after the treatment of EGF and TGF- alpha. The expression of EGFR decreased and activated EGFR expression increased in 30 minutes to 1 hour but decreased subsequently. When the cells treated with EGF, there were no change in beta-and gamma-catenin expression with there dependent manner. The tyrosine phosphorylation of beta-and gamma-catenin increased in 30 minutes to 1 hour but decreased subsequently with activated EGFR. CONCLUSION: This study showed that an activated EGFR which has involved with tyrosine phosphorylation of beta- and gamma-catenin influenced by growth factors rather than expression of E-cadherin, has a role in the invasion and metastasis of the cervical cancer.
Blotting, Western
;
Cadherins*
;
Cell Culture Techniques
;
Cell Line*
;
Epidermal Growth Factor*
;
Epithelial Cells
;
gamma Catenin*
;
Immunoprecipitation
;
Intercellular Signaling Peptides and Proteins
;
Membranes
;
Neoplasm Metastasis
;
Phosphorylation*
;
Transforming Growth Factor alpha*
;
Tyrosine*
;
Uterine Cervical Neoplasms*
2.Metabolic Syndrome and Oxidative Stress, Antioxidants.
Journal of the Korean Academy of Family Medicine 2006;27(10):773-781
No abstract available.
Antioxidants*
;
Oxidative Stress*
3.The Analysis of Actual Condition for Implementation of Dental Clinic Infection Prevention Standards in the Hospital.
Korean Journal of Nosocomial Infection Control 2011;16(2):37-44
BACKGROUND: The purpose of study to present the basic data for enhancing the performance level of the dental clinic infection prevention through the actual condition of the implementation for dental treatment infection prevention aimed at dental hygienists in each hospital. METHODS: We carried out a survey targeting 69 dental hygienists in the dental college hospital, 42 in the dentist of the Dentistry in General Hospital, 51 in the dental clinic and 38 in the dentist's office and the medical institutions surveyed, and we used frequency analysis and chi-square analysis by using SPSS (Ver. 18.0). RESULTS: The experiences wounded in the body by sharp instruments or needles contaminated with patients' blood or saliva were on average in 86.5% of the dental hygienists, and people who disinfected the wound with disinfectants after washing their own hands and squeezing blood as the method to fight the wound was 73.4%. The dental clinic was the highest in case of the rate of wearing gloves, and the dental clinic was the highest in the case of the rate of wearing a mask. The use of the liquid soap containing antimicrobial was the highest as 28.0% in the dental college hospital in case of 'the cleanser to be used for washing their own hands'. In the multiple response for dental treatment mechanical equipment management apparatus and the actual condition to be equipped with medicines and the survey in sterilization, Povidone was the highest as 28% in the dental college, and surface disinfectants were being used in some hospitals. CONCLUSION: To prevent the infection in the consultation room, dental hygienists should thoroughly recognize Dental Treatment Infection Prevention Standards, and the research measures or administrative support of the government and the organs concerned is needed a lot.
Dental Clinics
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Dental Hygienists
;
Dentistry
;
Dentists
;
Disinfectants
;
Hand
;
Hospitals, General
;
Humans
;
Masks
;
Needles
;
Povidone
;
Saliva
;
Soaps
;
Sterilization
4.Understanding of Alzheimer's Disease through Illustrative Cases.
Seung Hye CHOI ; Ae Young LEE ; Sang Yun KIM
Journal of the Korean Medical Association 2002;45(4):368-377
Alzheimer's disease (AD) is an age-related disorder. Both the prevalence and the incidence of AD double approximately every 5 years after the age of 60. This is a particularly serious problem considering that the Korean elderly population is rapidly growing. We present three illustrative cases of AD in mild, moderate, and advanced stages, respectively. Their initial symptoms were memory impairment which was followed by language disturbance and visuospatial dysfunction. Abnormal behaviors such as delusion and aggression occurred in moderate and severe cases. The presence and severity of dementia in these cases were established by history taking, neurological examination, standardized mental status assessment, and neuropsychological test. Laboratory investigations showed no abnormalities that could account for the cognitive deficits. Brain CT or MRI findings of the patients wee not remarkable except for a diffuse atrophy demonstrated by increased ventricular volume, narrowed gyri, and widened sulci, As an addendum, risk factors, clinical symptoms, clinical diagnosis, and natural history of AD were reviewed.
Aged
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Aggression
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Alzheimer Disease*
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Atrophy
;
Brain
;
Cognition Disorders
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Delusions
;
Dementia
;
Diagnosis
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Humans
;
Incidence
;
Magnetic Resonance Imaging
;
Memory
;
Natural History
;
Neurologic Examination
;
Neuropsychological Tests
;
Prevalence
;
Risk Factors
5.Transformational Experience of a Student Nurse with Diabetes: A Case Study.
Hye Jung CHOI ; Young Sang HONG
Journal of Korean Academy of Nursing 2007;37(2):192-200
PURPOSE: The current study was aimed to investigate the transformational experience of a female student nurse living with type 1 diabetes. METHODS: A case study of a 24-year-old diabetes patient was conducted, with interviews concerning the evolving process she had lived through during the period from her later high school years to her graduation from nursing college. RESULTS: Followings were identified as 5-transformation process: With her diabetes-related limitation, the participant experienced 'conflict involving choosing a college and major'. The participant tried to be in charge of managing her diabetes and stepped forward to 'adaptation to college life as a new environment', and she learned more about the process of 'evolving awareness of caring' and developed herself further through the process of 'integration of the nurse identity into self-identity', and finally through the process of 'progression and preparation for getting a job' she achieved her goals, being positive about the future. CONCLUSIONS: The results of the study can provide individuals with diabetes a way of self-management and help the patients and their families in diabetes education. Further research will be needed to refine the results of this study and to learn more about the experiences of patients with type I diabetes in college years.
*Adaptation, Psychological
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Adult
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Career Choice
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Conflict (Psychology)
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Diabetes Mellitus, Type 1/*psychology
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Empathy
;
Female
;
Humans
;
Job Application
;
Korea
;
Social Identification
;
Students, Nursing/*psychology
6.Lesser Omental Internal Hernia with Strangulation: A Case Report.
Sun Wha LEE ; Hye Young CHOI ; Seung Yon BAEK
Journal of the Korean Radiological Society 1994;31(6):1133-1136
Internal hernia is an unusual cause of the intestinal obstruction. The advances of CT make the diagnosis more easier than in the past. We report one case of internal hernia with herniation of the ileum into the lesser omenturn. The diagnosis could be made when abdominal radiographs showed fixed clustering of the small bowel loops in upper abdomen medial to the stomach. CT and ultrasound showed characteristic interposition of the ileum between the left hepatic lobe and the stomach.
Abdomen
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Diagnosis
;
Hernia*
;
Ileum
;
Intestinal Obstruction
;
Stomach
;
Ultrasonography
7.Erosion of the transverse foramina of Korean axis.
Byoung Young CHOI ; Hye Yeon LEE ; In Hyuk CHUNG
Korean Journal of Physical Anthropology 1993;6(2):239-248
No abstract available.
Axis, Cervical Vertebra*
8.Relationships among Premenstrual Syndrome, Perfection and Anxiety among the College Student.
Hye Seon CHOI ; Eun Dong LEE ; Hye Young AHN
Korean Journal of Women Health Nursing 2013;19(4):265-274
PURPOSE: The purpose of this study was to compare the premenstrual syndrome (PMS) and the relationships among PMS, perfection, and anxiety in nursing college students and general college students. METHODS: The questionnaire survey was carried out with a convenience sample of 215 college students. The MDQ (Menstrual Distress Questionnaire), APS-R (Almost Perfect Scale-Revised), STAI (State and Trait Anxiety Inventory) were used. The data were collected from June to October, 2011. Data analysis included frequency, chi2-test, t-test, and Pearson correlation. RESULTS: There were significant differences in PMS and perfection but there not in anxiety between nursing college students and general college students. There were significant correlations among PMS, perfection and anxiety in nursing college students, and there were significant correlations among PMS and anxiety in general college students but not significant correlations among PMS and perfection. CONCLUSION: The findings suggest that to provide effective interventions for PMS in college students, it is necessary to design nursing interventions that consider perfection and anxiety.
Anxiety*
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Female
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Humans
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Menstruation
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Nursing
;
Premenstrual Syndrome*
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Surveys and Questionnaires
;
Statistics as Topic
9.Effects of Mothers Involved in Dental Health Program for Their Children.
Journal of Korean Academy of Nursing 2012;42(7):1050-1061
PURPOSE: The purpose of this study was to identify the effects of mothers' involvement in a dental health program for their elementary school children. METHODS: This study was a non-equivalent control group pre-post test design in which knowledge and behaviors related to dental health, perceived benefits and barriers, self-efficacy and plaque control scores were compared between the experimental group (n=26) for whom the dental health program included the direct involvement of the mothers, and the control group (n=24) for whom knowledge related to dental health was provided through brochures. RESULTS: Scores for the experimental group in which the mothers were involved in the dental health program were significantly higher for knowledge, behaviors in dental health, self-efficacy and plaque control compared to the control group. CONCLUSION: Results of this study suggest that mothers involvement in the dental health program is effective in reinforcing dental health enhancing behavior in elementary school children.
Child
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Dental Caries
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Dental Plaque/prevention & control
;
Female
;
Health Knowledge, Attitudes, Practice
;
Humans
;
Male
;
Mothers/*psychology
;
*Oral Health
;
Pamphlets
;
Program Evaluation
;
Self Efficacy
10.A Case of Juvenile Dermatomyositis .
Hye Won YOM ; Ji Hye PARK ; Jeong Wan SEO ; Hye Young CHOI ; Hesoo GU ; Sun Wha LEE ; Yun Jong KANG
Journal of the Korean Child Neurology Society 2003;11(1):178-183
Juvenile dermatomyositis is an uncommon autoimmune disease with classic heliotrope discoloration of eyelids, erythematous skin rash of joints and proximal muscle weakness. Quite different from adults, malignancy is rarely accompanied in juvenile dermatomyositis. However vasculitis, muscle atrophy, calcification and gastrointestinal involvement are often observed in juvenile dermatomyositis. A six year old boy was admitted with chief complaints of general weakness and skin rash. Muscle biopsy was performed which was consistent with dermatomyositis. The patient was treated with intravenous immunoglobulin, steroid, methotrexate and physiotherapy. We report a case of juvenile dermatomyositis.
Adult
;
Autoimmune Diseases
;
Biopsy
;
Dermatomyositis*
;
Exanthema
;
Eyelids
;
Humans
;
Immunoglobulins
;
Joints
;
Male
;
Methotrexate
;
Muscle Weakness
;
Muscular Atrophy
;
Vasculitis