1.Apoptosis and Cell Cycle Arrest with EGF, TGF- a and TGF- 8 in Cervical Cancer Cell Lines .
Su Yeon KIM ; Hye Sung MOON ; Hye Won CHUNG ; Hye Young PARK ; Seung Chul KIM
Korean Journal of Gynecologic Oncology and Colposcopy 1999;10(1):58-66
BACKGROUND: EGF and TGF-a are ligands for the EGF-receptor and act as mitogens for a variety of tissues. TGF-a, in particular, has been implicated as an autocrine growth factor for several cancer cell lines. TGF-B exerts an inhibitory effect on the growth of most epithelial cell types, and the loss of responsiveness to this growth inhibition has been implicated in the development of a variety of human cancers. In the present study, we evaluate whether EGF, TGF-a and TGF-B modulate apoptosis and cell cycle progression in cervical cancer cell lines. MATERIALS & METHODS: The effect of EGF, TGF-a and TGF-B on apoptosis and cell cycle such as CaSki and HeLa cell lines was analysed by flow cytometry RESULTS: 1. TGF-B did not induce apoptosis in CaSki and HeLa cell lines. 2. TGF-B as well as EGF, TGF-a, did not affect the process of apoptosis significantly. 3. The time to occur apoptosis was different between CaSki and HeLa cells treated by growth factots. 4. G1 phase was the checkpoint in CaSki and HeLa cells treated with TGF-B. CONCLUSION: These results suggest that TGF-B as well as EGF, TGF-a does not induce apoptosis and cell growth inhibition.
Apoptosis*
;
Cell Cycle Checkpoints*
;
Cell Cycle*
;
Cell Line*
;
Epidermal Growth Factor*
;
Epithelial Cells
;
Flow Cytometry
;
G1 Phase
;
HeLa Cells
;
Humans
;
Ligands
;
Mitogens
;
Uterine Cervical Neoplasms*
2.Perceptions and Use of Premium Snacks and Associated Factors in School Aged Children and Their Mothers in Kyung-Ki Area.
Hye Young YANG ; Hyo Suk LEE ; Jayong CHUNG
Korean Journal of Health Promotion 2014;14(3):121-130
BACKGROUND: The purpose of this study was to examine the perceptions and use of premium snacks (PS) in school aged children. In addition, the influence of their mothers attitude toward the PS use on the children's PS eating behavior was examined. METHODS: 337 boys and 292 girls and their mothers (n=535) were participated in this study. Participants were recruited from two elementary schools located in Kyung-ki area. Information on general characteristics, the frequencies of general snack eating and associated factors, and the frequencies of PS eating and associated factors were obtained by a self-administered questionnaire. RESULTS: The mean frequency of PS eating were 1.12 times/wk in boys and 0.98 times/wk in girls. The PS eating frequency was positively associated with the frequency of eating general snacks in boys and the money spent on purchasing general snacks in girls. The main reasons for eating PS were 'health' followed by 'taste'. The frequencies of general snack eating and those of PS use in mothers were highly associated with the frequencies of eating PS in children. Further, the perceptions on PS in mothers were significantly correlated with the frequencies of PS eating both in boys and in girls, although correlation coefficients were somewhat weak. CONCLUSIONS: Our study showed that mother's snack eating behavior and perceptions can affect their children's PS uses. Our findings suggest that the education toward the mother's eating behavior and nutrition knowledge are important in improving child's eating behavior including reasonable and healthy snack choices.
Child*
;
Eating
;
Education
;
Feeding Behavior
;
Female
;
Gyeonggi-do
;
Humans
;
Mothers*
;
Snacks*
;
Surveys and Questionnaires
3.Comparison of Oxygenation and Lung Damage of High Frequency Flow Interruption to Conventional Ventilation in Surfactant Deficient Rabbits.
Chang Keun KIM ; Churl Young CHUNG ; Hye Jae CHO
Journal of the Korean Pediatric Society 1995;38(5):591-601
No abstract available.
Lung*
;
Oxygen*
;
Rabbits*
;
Ventilation*
4.Erosion of the transverse foramina of Korean axis.
Byoung Young CHOI ; Hye Yeon LEE ; In Hyuk CHUNG
Korean Journal of Physical Anthropology 1993;6(2):239-248
No abstract available.
Axis, Cervical Vertebra*
5.mRNA Expression of Thrombospondin-1 and -2 in Severe Endometriosis Patients in Korean Women.
Sung Eun HUR ; Ji Young LEE ; Hye Sung MOON ; Hye Won CHUNG
Korean Journal of Fertility and Sterility 2005;32(3):253-260
OBJECTIVE: We investigated the expression of TSP-1 and -2 in eutopic endometrium of advanced endometriosis and control patients. METHODS: The 33 endometriosis patients and 32 controls were enrolled. Endometrial samples were obtained from 65 premenopausal women aged 29-44 years, undergoing laparoscopic surgery or hysterectomy for non-malignant lesions. Sufficient samples were collected from 33 patients with endometriosis stage III and IV and 32 controls without endometriosis confirmed by laparoscopic surgery. The mRNA expression from eutopic endometrium for TSP-1 and -2 were analyzed by RT-QC PCR. RESULTS: The mRNAs of TSP-1 and -2 were expressed in eutopic endometrium from endometriosis and normal controls throughout the menstrual cycle. There were no significant differences in expression of TSP-1 and TSP-2 in eutopic endometrium between controls and endometriosis patients. CONCLUSION: Our results indicated that TSP-1 and -2 had no crucial role compared to other molecules in the regulation of angiogenesis. These findings also suggest that dysregulation of other angiogenic regulators would be concerned in pathophysiologic role in endometriosis development.
Endometriosis*
;
Endometrium
;
Female
;
Humans
;
Hysterectomy
;
Laparoscopy
;
Menstrual Cycle
;
Polymerase Chain Reaction
;
RNA, Messenger*
;
Thrombospondin 1
;
Thrombospondins
6.mRNA Expression Differences of uPA, uPAR in Eutopic Endometrium of Advanced Stage Endometriosis Patients.
Sung Eun HUR ; Ji Young LEE ; Woon Jung LEE ; Hye Sung MOON ; Hye Won CHUNG
Korean Journal of Fertility and Sterility 2006;33(4):229-236
OBJECTIVE: We investigated the expression of uPA and uPAR in eutopic endometrium of advanced stage endometriosis and control patients. METHODS: The 33 endometriosis patients and 32 controls were enrolled. Endometrial samples were obtained from 65 premenopausal women aged 29~44 years, undergoing laparoscopic surgery or hysterectomy for non-malignant lesions. Sufficient samples were collected from 33 patients with endometriosis stage III and IV and 32 controls without endometriosis confirmed by laparoscopic surgery. The mRNA expression of uPA and uPAR from eutopic endometrium were analyzed by RT-QC PCR. RESULTS: The mRNAs of uPA and uPAR were expressed in eutopic endometrium from endometriosis and normal controls throughout the menstrual cycle. Uterine endometrium from women with endometriosis expresses significantly (p<0.05) higher levels of u-PA mRNA than endometrium from normal women without endometriosis in the proliferative phase. There were no significant differences in expression of uPAR in eutopic endometrium between controls and endometriosis patients. CONCLUSION: These results suggest that eutopic endometrium from endometriosis patients may be more invasive and prone to peritoneal implantation because of greater u-PA mRNA expression than endometrium from women without endometriosis. Thus, increased proteolytic activity may be one etiology for the invasive properties of the endometrium resulting in the development of endometriosis.
Endometriosis*
;
Endometrium*
;
Female
;
Humans
;
Hysterectomy
;
Laparoscopy
;
Menstrual Cycle
;
Polymerase Chain Reaction
;
Proteolysis
;
RNA, Messenger*
;
Urokinase-Type Plasminogen Activator
7.Bacterial meningitis in newborn and infant: Correlation between organism, CT findings and clinical outcome.
Hye Young CHOI ; Young Seo PARK ; Shi Joon YOO ; Dae Chul SUH ; Young Kyo CHUNG
Journal of the Korean Radiological Society 1993;29(2):312-318
Bacterial meningitis results in significant neurologic dificits despite in spite of much effort in the treatment of the disease. This study was performed to determine the incidence of caustive organisms and to correlate between the organisms and computed tomographic (CT) findings with clinical outcome of bacterial meningitis in newborns and infants. We analyzed the brain CT and clinical records of 15 infants who had been diagnosed as bactrial meningitis by CSF culture. We found that the most common organisms were Group B streptococcus in neonates withou no neurologic complications in all but one and Hemophilus influenza in infants whose clinical outomes were poor in all except one. CT findings related with poor prognosis in this study were cerebral edema, basal cistermal obliteration & enhancement, and cerebral infarction on initial CT and ventriculomegaly on follow-up CT. We concluded that CT diagnosed intracranial complications of bacterial meningitis well and could contributed to better treatment of bacterial meningitis.
Brain
;
Brain Edema
;
Cerebral Infarction
;
Follow-Up Studies
;
Haemophilus
;
Humans
;
Incidence
;
Infant*
;
Infant, Newborn*
;
Influenza, Human
;
Meningitis
;
Meningitis, Bacterial*
;
Prognosis
;
Streptococcus
8.Korean and Chinese University Students' Importance and Performance Analysis for Quality Attributes by Coffee Type in Daejeon.
Hye Young KIM ; Hye Kyung CHUNG ; Hae Young LEE
Korean Journal of Community Nutrition 2011;16(4):511-524
The purposes of this study was to investigate importance and performance for coffee quality attributes in Korean and Chinese university students by 3 types of coffee; coffee in coffee houses, coffee in vending machines and canned/bottled coffee. Survey was done for 360 students in Daejeon with developed questionnaires including general information, favorite type of coffee and 15 quality attributes of coffee. Finally, data from 168 Korean and 126 Chinese university students was used for statistical analysis by SPSS 18.0 package program. Results of this study were as follows: for favorite type of coffee, the rates of coffee in coffee houses, coffee in vending machines and canned/bottled coffee were 43.0% 34.8% and 22.2% in Korean students and 8.7%, 78.0%, and 12.2% in Chinese students, respectively. Comparing Chinese students and Korean students showed higher importance for several quality attributes of coffee in coffee house (p < 0.05), but lower importance for most quality-attributes of coffee in vending machine (p < 0.05) and canned/bottled coffee (p < 0.05). According to importance and performance analysis, strategies for increasing satisfaction were to improve 'price' of canned/bottled coffee in Korean students, and 'taste', 'volume', 'concentration' and 'variety' of canned/bottled coffee and 'freshness' of coffee in vending machine in Chinese students. These results suggested that differentiated marketing plans between Korean and Chinese university students should be needed in coffee industry.
Asian Continental Ancestry Group
;
Coffee
;
Humans
;
Marketing
;
Surveys and Questionnaires
9.A Case of Multiple Brain Abscess Complicated to Purulent Meningitis in Newborn.
Kui Ja KIM ; Hye Young CHUNG ; Tae Chan KWON ; Young Dae KWON ; Chin Moo KANG
Journal of the Korean Pediatric Society 1983;26(7):717-721
No abstract available.
Brain Abscess*
;
Humans
;
Infant, Newborn*
;
Meningitis*
10.A Case of Dysgerminoma.
Dong Won KIM ; Hye Young CHUNG ; Seung Il KIM ; Young Hyun KWACK
Journal of the Korean Pediatric Society 1984;27(10):1018-1021
No abstract available.
Dysgerminoma*