1.Extract from Zanthoxylum piperitum Induces Apoptosis of AGS Gastric Cancer Cells Through Akt/MDM2/p53 Signaling Pathway.
Ye Seul PARK ; Gun He NAM ; Kyung Jo JO ; Hye Won KAWK ; Sang Yung KIM ; Young Min KIM
Chinese journal of integrative medicine 2021;27(10):752-759
OBJECTIVE:
To determine the effect of Zanthoxylum piperitum extracet (ZPE) on apoptosis and analyze anticancer substances in ZPE, changes in proteins related to apoptosis, and pathological changes in tumors in mouse.
METHODS:
Fifteen 4-week-old female BALB/c nu/nu mice were divided into 3 groups depending on ZPE dose, with 5 in each group. AGS gastric carcinoma cells (1 × 10
RESULTS:
High performance liquid chromatography (HPLC) analysis showed that ZPE contained organic sulfur compounds such as alliin and S-allylcysteine. MTT assay results revealed that ZPE (10-85 µ g/mL) could effectively inhibit the growth of AGS gastric cancer cells at higher concentrations (P<0.05, P<0.01). The annexin V & dead cell staining assay and cell cycle arrest assay confirmed a dose-dependent increase in the apoptosis rate and G
CONCLUSION
ZPE decreases AGS cell proliferation and induces apoptosis by inhibiting Akt and MDM2 expression.
Animals
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Apoptosis
;
Cell Line, Tumor
;
Cell Proliferation
;
Female
;
Mice
;
Mice, Inbred BALB C
;
Plant Extracts/therapeutic use*
;
Proto-Oncogene Proteins c-akt/metabolism*
;
Signal Transduction
;
Stomach Neoplasms/drug therapy*
;
Tumor Suppressor Protein p53/metabolism*
;
Zanthoxylum/metabolism*