1.PCR-RFLP patterns of four isolates of Trichinella for rDNA ITS1 region.
Hye Soo KWON ; Myung Sook CHUNG ; Kyoung Hwan JOO
The Korean Journal of Parasitology 2001;39(1):43-48
We have studied the genetic differences among four isolates of Trichinella including a new strain of Trichinella spiralis (ISS 623) recently found from a human case who took a badger in Korea. Because they have a different host origin and came from geographically separated regions, we supposed the genetic pattern of the isolates might be different as had been previously reported. It was analysed by PCR-RFLP analysis of the rDNA repeat that can readily distinguish a species or strain from others. Isolated genomic DNA of each isolate of Trichinella larvae was amplified with ITS1 specific primers and digested with restriction endonucleases. The PCR product of ITS1 was confirmed using Southern blot analysis to be a 910 bp fragment. The restriction fragments of each isolate had variable patterns when it was digested with Rsa 1 only. According to the RFLP patterns, the estimated genetic divergence between each isolate was different. In conclusion, four isolates of Trichinella including a new strain of T. spiralis obtained from a Korean patient may have genetic differences in the ITS1 region and the Shanghai isolate was genetically more similar to the Japanese unknown isolate than others in the ITS1 region.
Animals
;
*DNA, Helminth
;
*DNA, Ribosomal
;
Human
;
Polymerase Chain Reaction
;
Polymorphism, Restriction Fragment Length
;
Repetitive Sequences, Nucleic Acid
;
Trichinella spiralis/*genetics/isolation & purification
2.Ureteral jets in patients with unilateral ureteral calculi: Using color doppler ultrasonography.
Hye Soo KWON ; Hae Won PARK ; Won Ja OH
Journal of the Korean Radiological Society 1993;29(3):486-491
Sonographic detection of ureteral jets entering the urinary bladder is a well-known procedure. Color Doppler ultrasound was used to image the ureteral jets in 28 patients with unilateral ureteral calculi proved by intravenous urography. Three major findings of ureteral jets were obtained in the affected ureter with a stone: less frequency (26 patients), weak reflection (20 patients), and lower grade in length (27 patients) that were significantly different from the unaffected side in the same patient. No significant differences were found between the ureteral jets and the degree of hydronephrosis or the location of a stone. In conclusion, these results suggest that ureteral jets should be checked routinely during the procedure of color Doppler ultrasonogram in patients with possible unilateral ureteral obstruction. Color Doppler examination of ureteral jets may be a important adjunct in routine pelvic ultrasonogram especially in patients who have absolute or relative contraindications of intravenous urography.
Humans
;
Hydronephrosis
;
Ultrasonography
;
Ultrasonography, Doppler, Color*
;
Ureter*
;
Ureteral Calculi*
;
Ureteral Obstruction
;
Urinary Bladder
;
Urography
3.Ureteral jets in patients with unilateral ureteral calculi: Using color doppler ultrasonography.
Hye Soo KWON ; Hae Won PARK ; Won Ja OH
Journal of the Korean Radiological Society 1993;29(3):486-491
Sonographic detection of ureteral jets entering the urinary bladder is a well-known procedure. Color Doppler ultrasound was used to image the ureteral jets in 28 patients with unilateral ureteral calculi proved by intravenous urography. Three major findings of ureteral jets were obtained in the affected ureter with a stone: less frequency (26 patients), weak reflection (20 patients), and lower grade in length (27 patients) that were significantly different from the unaffected side in the same patient. No significant differences were found between the ureteral jets and the degree of hydronephrosis or the location of a stone. In conclusion, these results suggest that ureteral jets should be checked routinely during the procedure of color Doppler ultrasonogram in patients with possible unilateral ureteral obstruction. Color Doppler examination of ureteral jets may be a important adjunct in routine pelvic ultrasonogram especially in patients who have absolute or relative contraindications of intravenous urography.
Humans
;
Hydronephrosis
;
Ultrasonography
;
Ultrasonography, Doppler, Color*
;
Ureter*
;
Ureteral Calculi*
;
Ureteral Obstruction
;
Urinary Bladder
;
Urography
4.Korean Version of the 14-Item Resilience Scale (RS-14) for University Students: A Validity and Reliability Study.
Journal of Korean Academy of Psychiatric and Mental Health Nursing 2014;23(4):226-232
PURPOSE: The purpose of this study was to verify the validity and reliability of a 14-item Resilience Scale (RS-14) by applying it to Korean university students. METHODS: The original English version of the RS-14 was translated into Korean, and the Korean version of the RS-14 was confirmed with back-translation. This was a methodological study, and data were collected from 273 students. Principal components factor analysis and Pearson correlation coefficients were used to test validity; Cronbach's alpha coefficient was used to test reliability. RESULTS: Factor analysis of 14 items revealed 2 factors with a total variance of 55.43%. The new scale correlated significantly with self-esteem (.48, p<.001) and depression (-.44, p<.001). Cronbach's coefficient was .905. CONCLUSION: The results indicate good validity and reliability for the Korean version of the RS-14. Future studies will need to further test this scale by applying it to different study populations.
Depression
;
Humans
;
Methods
;
Reproducibility of Results*
5.Major patterns of inflammatory sinonasal diseases on CT.
Won Ja OH ; Eun Kyung YOUN ; Young Uk LEE ; Hye Soo KWON
Journal of the Korean Radiological Society 1993;29(5):901-906
Paranasal sinus CT is known as the most effective imaging modality in the evaluation of inflammatory sinonasal diseases and can depict the distribution, causative lesions obstructing main drainge route, and associated findings. Recently, functional endoscopic sinus surgery has been widely used for the evaluation and treatment. Before operation, PNS CT has been routinely used to evaluate the paranasal sinuses and mucociliary drainage route. The authors analyzed the PNS CT findings of 3156 cases in 1578 patients with chronic sinusitis symptoms. Sinonasal inflammatory diseases were categorized into 5 patterns according to the obstruction sites. They were 1) infundibular (10%, 316/3156), 2) ostimeatal unit (41%, 1294/3156), 3) sphenoethmoidal recess (12%, 379/3156), 4) sinonasal polyposis (30%, 946/3156) , and 5) unclassifiable (6%, 190/3156) patterns. The main causes for infundibular obstruction in order of frequency were inflammatory mucosa, enlarged ethmoidal bulla and Haller's cell. With respect to the middle meatus obstruction, is main causes in the order of frequency were polypoid lesion, inflammatory mucosa and medially deviated uncinate process. In particular, sinonasal polyposis showed one or more of the characteristic associated findings of infundibular enlargement, air-fluid level, ethmoidal sinus was bulging and bony deossification or sclerosis as well as sinonasal polypoid change. In conclusion, the inflammatory sinonasal diseases were classified into five patterns, and the causative lesions or anatomic variations were efficiently detected by the PNS CT. Furthermore, it could provide a guidance for proper management of the sinusitis including functional endoscopic sinus surgery.
Drainage
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Humans
;
Mucous Membrane
;
Paranasal Sinuses
;
Sclerosis
;
Sinusitis
6.A Phenomenological Study on Oncology Adaptation Experiences of New Nurses.
Asian Oncology Nursing 2014;14(2):127-138
PURPOSE: The purpose of this study was to understand the meaning and nature of adaptation experiences among newly employed nurses in oncology ward. METHODS: The present study adopted a phenomenological method. The participants were 9 nurses employed for less than one year. Data were collected by using in-depth interviews. The question of this study was, "What are your experiences as a newly employed nurse in oncology?" RESULTS: 150 significant statements were extracted from the data, and these were clustered into 5 main categories: a 'Difficulty in performing the work of a cancer ward', 'problems with interpersonal relationships', 'striving to be a good nurse', 'satisfaction and rewarding nursing', and 'adapted oneself to be a nurse'. CONCLUSION: Newly employed nurses in oncology ward experienced and perceived feelings of difficulty in performing the work of a cancer ward, problems with interpersonal relationships, striving to be a good nurse, satisfaction and rewarding nursing, and adapting oneself to be a nurse. It is necessary to understand the experiences of new nurses, and to provide strategies that could help new nurses effectively adapt and find the meaning in their adaptation to work in oncology wards. Further research is needed on development of a program focusing on training to improve practicing ability and interpersonal relationships skills.
Nursing
;
Qualitative Research
;
Reward
7.Knowledge and Practice of Fever Management by Mothers of Preschool Children at Home.
Child Health Nursing Research 2017;23(2):127-136
PURPOSE: This study was done to identify the level and correlation of knowledge and practice of fever management at home and provide data for the development of an intervention program to improve the fever management ability of mothers of preschool age children. METHODS: Participants were 112 mothers with children between 1 and 6 years of age and attending one of three daycare centers located in J city. The participants had a history of using medication and tepid massage. Data were collected during April, 2016. RESULTS: The mean scores were 27.55 (correct answer rate 70.64%) of 39 for knowledge, and 75.93 of 92 for fever management practice. There were significant differences in scores for knowledge according to the number of children and number of visits to doctors for treatment of a fever. There were no differences in scores for practice. There was a positive correlation between knowledge and practice. CONCLUSION: Results indicate that mothers' knowledge and practice of childhood fever management were insufficient to provide accurate management of childhood fevers. Therefore, to improve the mothers' ability to provide appropriate fever management, educational strategies that focus on increasing knowledge need to be developed.
Child
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Child, Preschool*
;
Fever*
;
Humans
;
Massage
;
Mothers*
8.Development and Verification of the Effect of Health Lifestyle Education Program for Cancer Prevention in Late School-aged Children.
Journal of Korean Academy of Child Health Nursing 2012;18(4):192-200
PURPOSE: This study was done to develop and test the effectiveness in late school-aged children of a healthy lifestyle education program for cancer prevention. METHODS: Participants were 26 students in the sixth grade of N elementary school in J City (experimental group) and 25 students in the sixth grade of D elementary school with same educational conditions (control group). The research design was a nonequivalent control group pretest-posttest design. Data collection and execution of the study were carried out from March 10 to May 19, 2011. For the experimental treatment, 7 weekly sessions of the healthy lifestyle education program for cancer prevention were given by the researcher. Posttest was done immediately following the conclusion of the program and 4 weeks later. Frequencies, percentages, chi2-test, t-test, repeated measures ANOVA and paired t-test with SPSS WIN 18.0 were used to analyze the data. RESULTS: Perceived barriers, perceived self-efficacy, activity related effect and family support significantly influenced cancer prevention knowledge and increases in healthy life styles. CONCLUSION: Results indicate that, as this program for cancer prevention was effective in increasing healthy lifestyle practices in these children, there is a need to further develop, test and implement programs in all schools.
Child
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Data Collection
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Humans
;
Life Style
;
Research Design
;
Child Health
9.Tetracycline Hydrochloride Sclerotherapy: Renal, Hepatic, Ovarian, and Perivesical cysts.
Ju Ho KIM ; Jae Bum YANG ; Jae Chul GONG ; Hye Soo KWON
Journal of the Korean Radiological Society 2000;43(5):551-556
PURPOSE: To assess the efficacy and resulting complications of tetracycline sclerotherapy in renal, hepatic, ovarian, and perivesical cysts. MATERIALS AND METHODS: We retrospectively reviewed 23 cases of benign cysts (16 renal, 4 hepatic, 2 ovarian, and 1 perivesical) in 22 patients in whom the condition was diagnosed or confirmed by either ultrasound, CT, or cytology, and who underwent percutaneous tetracycline sclerotherapy. Using a 21-gauge Chiba needle, the target cyst was punctured under ultrasound guidance. Prior to the injection of 1500 mg of tetracycline diluted in 5 ml of normal saline, almost all the cystic content was aspirated, and at the end of the procedure the tetra-cycline was left in the cyst. During a period of between 3 and 22 months, 18 of the 23 cases were followed up. RESULT: In six of the 18 cases followed up, the cysts either decreased in size by 10%, or collapsed completely. In seven cases a collapse of over 50% was noted, and in the remaining five the cyst recurred. In one of these, complete collapse occurred after retreatment at ten months, and the patient with a perivesical cyst underwent surgery six months after recurrence. Thus, treatment was effective (a collapse of at least 50%) in 13 of 18 cases (72.2%). This total of 13 comprised ten of 12 renal cysts (83.3%), two of two ovarian (100%), and one of three hepatic (33.3%). Percutaneous therapy was unsuccessful in five cases (two hepatic cysts, one renal, one para-pelvic and one perivesical). Complications occurring during the procedure or follow-up period included discomfort or mild pain, vomiting, and transient fever, though these subsided within 24 hours. In one patient with severe pain, this subsided after four days. CONCLUSION: As single-shot injection of tetracycline provides safe and effective treatment for renal and ovarian cysts, but for hepatic cysts is unsuccessful.
Female
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Fever
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Follow-Up Studies
;
Humans
;
Needles
;
Ovarian Cysts
;
Recurrence
;
Retreatment
;
Retrospective Studies
;
Sclerotherapy*
;
Tetracycline*
;
Ultrasonography
;
Vomiting
10.Validity and Reliability of an Instrument for Predictive Nursing Intention for SARS Patient Care.
Hye Ra YOO ; Bo Eun KWON ; Yon Soo JANG ; Heun Keung YOUN
Journal of Korean Academy of Nursing 2005;35(6):1063-1071
PURPOSE: This study was done to develop and test validity and reliability of on instrument for predicting nursing intention for SARS patient care. METHOD: The psychometric properties of a SARS patient care attrition prediction tool, based on the Theory of Planned Behavior, were examined in this study. The Three-phase design involved a) salient beliefs generated from clinical nurses (n=43) b) content validation by expert panel evaluations(n=5) c) face validation by plot testing (n=10) d) and instrument validation in a cross sectional survey (n=299). Psychometric analysis of survey data provided empirical evidence of the construct validity and reliability of the instrument. RESULT: Principal component analysis verified the hypothesized 6-factor solution, explaining 68.2% of variance, and Alpha coefficients of .7538 to .9389 indicated a high internal consistency of the instrument. CONCLUSION: The instrument can be used by nurse administrators and researcher to assess clinical nurses' salient beliefs about caring for SARS patients, guide tailored intervention strategies to effective caring, and evaluate the effectiveness of interventions.
Severe Acute Respiratory Syndrome/*nursing
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Reproducibility of Results
;
*Questionnaires
;
Nursing Methodology Research
;
Nursing Care
;
Nurses/*psychology
;
Humans
;
Female
;
Attitude of Health Personnel
;
Adult