2.PCR-RFLP patterns of four isolates of Trichinella for rDNA ITS1 region.
Hye Soo KWON ; Myung Sook CHUNG ; Kyoung Hwan JOO
The Korean Journal of Parasitology 2001;39(1):43-48
We have studied the genetic differences among four isolates of Trichinella including a new strain of Trichinella spiralis (ISS 623) recently found from a human case who took a badger in Korea. Because they have a different host origin and came from geographically separated regions, we supposed the genetic pattern of the isolates might be different as had been previously reported. It was analysed by PCR-RFLP analysis of the rDNA repeat that can readily distinguish a species or strain from others. Isolated genomic DNA of each isolate of Trichinella larvae was amplified with ITS1 specific primers and digested with restriction endonucleases. The PCR product of ITS1 was confirmed using Southern blot analysis to be a 910 bp fragment. The restriction fragments of each isolate had variable patterns when it was digested with Rsa 1 only. According to the RFLP patterns, the estimated genetic divergence between each isolate was different. In conclusion, four isolates of Trichinella including a new strain of T. spiralis obtained from a Korean patient may have genetic differences in the ITS1 region and the Shanghai isolate was genetically more similar to the Japanese unknown isolate than others in the ITS1 region.
Animals
;
*DNA, Helminth
;
*DNA, Ribosomal
;
Human
;
Polymerase Chain Reaction
;
Polymorphism, Restriction Fragment Length
;
Repetitive Sequences, Nucleic Acid
;
Trichinella spiralis/*genetics/isolation & purification
3.Heart size in new-born infants
Soo Won KIM ; Yun Jeong YU ; Hye Kyung CHUNG
Journal of the Korean Radiological Society 1985;21(5):751-754
Cardiac size of 291 new-born infants was measured using the method illustated on Fig 1. Among the 291 infants,53 were asphyxiated, and asphxia was only regared from Apgar score below 6 on 1 min. and 5 min. Remaining 238infants were normal, and classified to group with lung abnormalities and without lung abnormalities on chest A-Pfilm. The results are as follows; 1. The average CTR. of normal group was 52.37(C/T1;54.89, C/T2;49.43, C/T3;49.15, C/T;55.97) 2. The average CTR. of asphxiated group was 54.91 (C/T1;57.13, C/T2;51.69, C/T3;51.94,C/T4;58.25) 3. Consequently, asphyxiated infants revealed larger cardiac size than normal infant group.
Apgar Score
;
Heart
;
Humans
;
Infant
;
Lung
;
Methods
;
Thorax
4.A Case of Ocular Rosacea.
Hye Jin WOO ; Hye Nam LEE ; Sung Woo CHOI ; Chung Won KIM ; Man Soo KIM
Korean Journal of Dermatology 1999;37(3):400-403
Ocular rosacea is the eye manifestations of rosacea, which involves the lids, conjunctiva, and cornea. A recent study revealed that all patients with cutaneous rosacea had some degree of ocular involvement which is often undiagnosed. It is difficult to make a diagnosis of ocular rosacea because it lacks clinical or pathological hallmarks. However, early diagnosis and treatment are emphasized to decrease morbidity and prevent potential blindness. We report a case of ocular rosacea in a 49 year-old man who showed blepharoconjunctivitis, keratitis, and cutaneous manifestations of persistent erythema, telangiectasia, and papules. The ocular symptoms and signs had improved significantly with tetracycline therapy.
Blindness
;
Conjunctiva
;
Cornea
;
Diagnosis
;
Early Diagnosis
;
Erythema
;
Eye Manifestations
;
Humans
;
Keratitis
;
Middle Aged
;
Rosacea*
;
Telangiectasis
;
Tetracycline
5.Clinicopathologic Features and HBsAg and HBeAg Expressions in Hepatitis B Virus-associated Glomerulopathy.
Hye Kyoung YOON ; Woo Yeong CHUNG ; Soo Jin JUNG ; Yong Hoon KIM ; Su Yung KIM
Journal of the Korean Society of Pediatric Nephrology 1998;2(1):50-59
Morphometry of nuclei of the benign and malignant prostatic lesions was performed to study the relationship between nuclear size and shape and the prognosis of prostatic adenocarcinoma. Fifty one cases of prostatic adenocarcinoma and 13 cases of benign prostatic hyperplasia were included to evaluate area, perimeter, Dmax, Dmin, and 5 form factors of the nuclei by image analyzer (Zeiss Ibas 2000) using hematoxylin-eosin stained slides. All analytic factors of nuclear size and shape were significantly different between benign lesions and adenocarcinomas. Increased nuclear size was associated with nu- clear irregularity, presence of metastasis, advanced clinical stage, and high Gleason's grade and score of prostatic adenocarcinoma. On Kaplan-Meier method, survival was decreased with older age, no hormonal treatment, stage D, high Gleason's grade and stage as well as with larger size and irregular shape of the nuclei. In conclusion, morphometry of nuclei of the prostate can be a helpful tool to differentiate between benign and malignant lesions. Nuclear morphology is thought to be associated with prognosis of prostatic adenocarcinoma.
Adenocarcinoma
;
Breast
;
Fibroadenoma
;
Hepatitis B e Antigens*
;
Hepatitis B Surface Antigens*
;
Hepatitis B*
;
Hepatitis*
;
Neoplasm Metastasis
;
Prognosis
;
Prostate
;
Prostatic Hyperplasia
6.Primary Tracheobronchial Amyloidosis: A Case Report.
Ho Jung KIM ; Yul LEE ; Soo Young CHUNG ; Mi Sook WON ; Ik YANG ; Hye Kyung AHN
Journal of the Korean Radiological Society 1995;32(1):121-123
Localized pulmonary amyloidosis is a rare disease characterized by a deposition of amyloid limited to the lungs. We report a case of primary tracheobronchial amyloidosis which resulted in collapse of left lung and was confirmed by the Congo-red staining.
Amyloid
;
Amyloidosis*
;
Lung
;
Rare Diseases
7.Primary Tracheobronchial Amyloidosis: A Case Report.
Ho Jung KIM ; Yul LEE ; Soo Young CHUNG ; Mi Sook WON ; Ik YANG ; Hye Kyung AHN
Journal of the Korean Radiological Society 1995;32(1):121-123
Localized pulmonary amyloidosis is a rare disease characterized by a deposition of amyloid limited to the lungs. We report a case of primary tracheobronchial amyloidosis which resulted in collapse of left lung and was confirmed by the Congo-red staining.
Amyloid
;
Amyloidosis*
;
Lung
;
Rare Diseases
8.Anesthetic Management of a patient with Primary Aldosteronism caused by Adrenal Adenoma.
Kyung Hye CHOI ; Heung Kwan CHUNG ; Il Soo KYOUN
Korean Journal of Anesthesiology 1994;27(11):1692-1697
Increased and inappropriate production of aldosterone from the adrenal gland is known as primary aldosteronism and leads to sodium retention with hypertension, suppression of plasma renin, and hypokalemia and its manifestations. It is due mainly to a solitary adenoma, bilateral hyperplasia, or rarely an adrenal carcinoms. Primary aldosteronism due to an adenoma is usually treated by surgical excision. Preoper-ative managements include the correction of hypokalemia, volume derangement, and metabolic alkalosis with spironolactone and the trestment of existing hypertension. The authors report a case of unilateral adrenalectomy and anesthetic considerations during perioperative periods.
Adenoma*
;
Adrenal Glands
;
Adrenalectomy
;
Aldosterone
;
Alkalosis
;
Humans
;
Hyperaldosteronism*
;
Hyperplasia
;
Hypertension
;
Hypokalemia
;
Perioperative Period
;
Plasma
;
Renin
;
Sodium
;
Spironolactone
9.Smooth muscle tumors of digestive tract: a clinicopathologic study of 40 cases-.
Dae Jig LEE ; Chang Soo KIM ; Eul Sam CHUNG ; Hye Kyung LEE
Journal of the Korean Cancer Association 1992;24(1):149-159
No abstract available.
Gastrointestinal Tract*
;
Muscle, Smooth*
;
Smooth Muscle Tumor*
10.Radiologic findings of male breast cancer: two cases report.
Soo Young CHUNG ; Sook Hyun KIM ; Sang Hoon BAE ; Hye Kyung AHN
Journal of the Korean Radiological Society 1993;29(5):1080-1083
Male breast cancer is very rare, with the incidence of 0.15-1% of all breast cancers and less than 1% of all cancers in men. The prognosis of male breast cancer is poorer than that of female because the median age of detection of the disease is in more late stage. It usually involves higher axillary lymph nodes. We report two cases of male breast cancer to describe characteristic mammographic and ultrasonographic findings and to compare with the findings of gynecomastia.
Breast
;
Breast Neoplasms, Male*
;
Female
;
Gynecomastia
;
Humans
;
Incidence
;
Lymph Nodes
;
Male
;
Male*
;
Prognosis