PURPOSE: Coronary arterial lesions after Kawasaki disease may cause coronary endothelial dysfunction without initial coronary dilatation, stenosis, and aneurysm. TTE (transthoracic echocardiography) is a non-invasive and widely used method in clinical practice. Adenosine is short acting drug and induces coronary vasodilatation. This study was designed to measure accurate coronary dysfunction by adenosine infusion. Coronary flow reserve(CFR) is defined as "the ratio of peak hyperemic to basal coronary flow velocity(CFV) in the proximal coronary vessel". CFR measurements has provided useful clinical and physiologic information in pediatric patients with congenital or acquired heart disease. METHODS: We examined 29 patients who had a history of Kawasaki disease. 19 patients discontinued low dose aspirin(5 mg/kg/d) after recovered from coronary dilatation and 10 patients have taken low dose aspirin because they had coronary dilatation. Adenosine was infused at 160 microgram/kg/min for 4 min. Diameter, velocity and integral of coronary artery were measured. RESULTS: Diameter and velocity were increased after adenosine infusion in both coronary arteries. A reduced CFR of both coronary arteries was noted in unrecovered group. CONCLUSION: CFR measured by TTE can predict the presence of significant coronary dysfunction in convalescent phase of Kawasaki disease.
Adenosine* ; Aneurysm ; Aspirin ; Constriction, Pathologic ; Coronary Vessels* ; Dilatation ; Echocardiography* ; Heart Diseases ; Humans ; Mucocutaneous Lymph Node Syndrome* ; Vasodilation

PURPOSE: Kawasaki disease is an acute systemic vasculitis syndrome of children. Although its etiology is largely unknown, epidemiological findings suggest that genetic factors play a role in the pathogenesis of Kawasaki disease. We investigated differentially expressed genes in Kawasaki patients. METHODS: we extracted bloods of Kawasaki patients in acute, subacute, convalescent phase and normal control group. Gene expressions were investigated by annealing control primer (ACP)-based GeneFishing PCR. Products were visualized after electrophoresis in 2% agarose gel and automatically sequenced. RESULTS: 165 differentially expressed genes were identified by using 120 ACPs. Expression of immunoglobulin kappa and lambda (DEG 20,45,54) significantly was down-regulated in acute and subacute phase of Kawasaki disease but up-regulated in convalescent phase. Apollon gene (DEG 64) was down-regulated in kawasaki patients. TNIK and other DEGs were also found. CONCLUSION: This findings suggest that different genetic change occur in the coarse of Kawasaki disease. Further study of differentially expressed gene using annealing control primer system may provide etiology and pathogenesis of Kawasaki disease.
Child ; Electrophoresis ; Gene Expression ; Humans ; Immunoglobulins ; Mucocutaneous Lymph Node Syndrome* ; Polymerase Chain Reaction ; Sepharose ; Systemic Vasculitis

Cutis verticis gyrata(CVG) is a morphologic syndrome of the scalp characterized by folds and furrows resembling the surface of brain. The primary form of cutis verticis gyrata is commonly associated with a neuropsychiatric condition and a secondary form occurs as a response of inflammatory or neoplastic process. The patient was a 1-day-old girl, who had a 15x11 cm sized, convoluted mass over whole scalp. Skin biopsy from the lesion was consistent with cerebriform intradermal nevus. The baby girl was followed up as an outpatient with monitoring of the scalp lesion for 2 years and showed improvement of folds and furrows of the lesion.
Biopsy ; Brain ; Female ; Humans ; Infant, Newborn* ; Nevus, Intradermal* ; Outpatients ; Scalp ; Skin

No abstract available.
Thrombocytopenia, Neonatal Alloimmune*

No abstract available.
Thrombocytopenia, Neonatal Alloimmune*

Pigmentation is induced by production of melanin in specialized organelles termed melanosomes and by transfer of these organelles from melanocytes to surrounding keratinocytes. The chemical basis of melanogenesis is relatively well known but the mechanism of melanosome transfer is not well studied. Various pigmentary disorders and cosmetic applications require the use of depigmenting agents. Currently available topical agents used for the reduction of pigmentation mainly include tyrosinase inhibitors and/or melanocyte-cytotoxic agents. Recently, several agents have been introduced to inhibit melanosome transfer from melanocytes to keratinocytes. However, an experimental model for melanosome transfer is not well established. In this study, a simple assay method using flow cytometry is described.
Cosmetics ; Flow Cytometry ; Keratinocytes ; Melanins ; Melanocytes ; Melanosomes ; Models, Theoretical ; Monophenol Monooxygenase ; Organelles ; Pigmentation

No abstract available.
Galectin 3* ; Skin*

No abstract available.
Activins* ; Regeneration*

OBJECTIVE: This is designing the part of Electronic Medical Record using HL7 Development Framework and Reference Information Model to realize the building medical standard data model for sharing medical record between heterogeneous hospital systems. METHODS: The process used development of HL7 specifications consists of the following seven activities: 1.Project initiation. 2.Requirements Documentation. 3.Specification Modeling. 4.Specification Documentation. 5.Specification Approval. 6. Specification Publication. 7.Implementation Profiling. Each activity is briefly described in the subsections that follow and described in detailed in the methodology chapters that follow this introduction.3. Result. The steps, after 4 step, needs to standardized the results. So we didn't followed that steps1). RESULTS: We got the diagrams at each steps of the HDF methodology: 1.A dynamic description. 2.A static description of the concepts involved in the business process. 3.A Use Case model which identifies the system involved in the actual HL7 data/information exchange1). CONCLUSION: It was confirmed that HL7 RIM could take in the domestic demands of medical records, and concrete methodology was applied in practice. It can be a good reference for the hospitals constructing new information system and for the enterprises developing medical information systems to apply the HL7 version 3 to their works.
Commerce ; Electronic Health Records* ; Health Level Seven* ; Health Status* ; Information Systems ; Medical Records ; Publications

BACKGROUND: There are different models of skin substitutes, but no skin substitutes have the characteristics of native skin. It was reported that the incubation of skin substitutes in medium containing ascorbic acid extends cellular viability and promotes formation of an epidermal barrier in vitro. OBJECTIVE: The purpose of this study is to observe the effects of ascorbic acid on the proliferation of keratinocytes and on the reconstruction of epidermis. MATERIALS AND METHODS: Normal human keratinocytes and fibroblasts were isolated and used for culturing living skin equivalent (LSE). RESULTS: When ascorbic acid was added, the expression of p63 and a6 integrin was definitely increased compared to control models. In addition, ascorbic acid increased the proliferation of normal human keratinocytes at a dose dependent manner. Especially, ascorbic acid induced the phosphorylation of ERK and up-regulation of EGF-R CONCLUSION: Results suggest that ascorbic acid is essential in the control of keratinocyte proliferation and basement membrane formation. Ascorbic acid-related keratinocytes proliferation is seemed to be mediated by ERK phosphorylation and EGF-R up-regulation.
Ascorbic Acid* ; Basement Membrane ; Epidermis ; Fibroblasts ; Humans ; Keratinocytes* ; Phosphorylation ; Skin* ; Skin, Artificial ; Up-Regulation