Phosphoinositide-specific Phospholipase C (PI-PLC) is a second messenger of signal transducer on cell membrane. In the previous study, PLC of bovine brain has been purified three isozymes. In this paper, uterus and seminal vesicle have been purified. Two peaks of PI-PLC activity were resolved when bovine uterus and seminal vesicle proteins were chromatographed on a DEAE and phenyl TSK 5PW HPLC column. Each two peak was compared with PI-PLC I, II and III from bovine brain and we got the retention time on HPLC. The peak fractions with PLC activity were tested homogeneity with brain PLC monoclonal antibodies (Mab). Mab-labeled affigels were bounded in the range of 73.8%~97.5% with PLC I, II and III. Homogeneity of fractions were revealed that DEAE F-1 and phenyl F-1-I were highest level of PLC III in uterus and seminal vesicle and DEAE F-2 and phenyl F-2-I were mixed PLC I and II.
Antibodies, Monoclonal ; Brain* ; Cell Membrane ; Chromatography, High Pressure Liquid ; Isoenzymes* ; Phospholipases* ; Second Messenger Systems ; Seminal Vesicle Secretory Proteins ; Seminal Vesicles* ; Transducers ; Type C Phospholipases* ; Uterus*

No abstract available.
Heart Septal Defects, Ventricular ; Jacobsen Distal 11q Deletion Syndrome* ; Pyloric Stenosis, Hypertrophic

No abstract available.
Fibrosis* ; Hypersplenism*

Background: Height loss is a simple clinical measure associated with increased fracture risk. However, limited data exists on the association between height loss and fracture risk in postmenopausal Korean women. It is unknown whether this association varies with age. Methods: Data on height loss over a 6-year period were collected from a community-based longitudinal follow-up cohort (Ansung cohort of the Korean Genome and Epidemiology Study). Incident fractures were defined based on self-reported fractures after excluding those due to severe trauma or toes/fingers. The association between incident fractures and height loss was investigated using a Cox proportional hazards model. Results: During a median follow-up of 10 years after the second visit, 259/1,806 participants (median age, 64 years) experienced incident fractures. Overall, a 1 standard deviation (SD) decrease in height (1.6 cm/median 5.8 years) was associated with 9% increased risk of fracture (hazard ratio [HR], 1.09; P=0.037), which lost statistical significance after adjustment for covariates. When stratified into age groups (50–59, 60–69, 70 years or older), a 1 SD decrease in height remained a robust predictor of fracture in the 50 to 59 years age group after adjusting for covariates (adjusted hazard ratio [aHR], 1.52; P=0.003), whereas height loss was not an independent predictor of fracture in the 60 to 69 (aHR, 1.06; P=0.333) or the 70 years or older age groups (aHR, 1.05; P=0.700; P for interaction <0.05, for all). Conclusion Height loss during the previous 6 years was associated with an increased 10-year fracture risk in postmenopausal women in their 50s.

Radilogy plays an important role in evaluating patients with suspected adrenal gland pathology. Morphologicdelineation of adrenal gland is especially valuable in patients with clinical and/or biochemical evidence of adisturbance in adrenal function. Many diagnostic radiologic methods are avilable for demonstrating adrenallesions. CT overcomes many of the disadvantages of these other radiologic techniques. The high degree of spatialand density resolution allows precise demonstration of the normal adrenal glands as well as detection of bothsmall and large tumors in almost all patients. So CT of adrenal gland is an excellet nonivasive screening methodand definitive imaging technique. The authers have investigated the capability of CT to image the nomral size,location and shape of both glands. Knowledge of the range of normal is useful for optimal interpretation of CTscans in patients with suspected aderenal pathology. We reviewed CT scan of 150 cases without evidence of adrenaldisease. The following results were obtained: 1. There were 90 male and 60 female patients. 2. Their ages rangedfrom 20 to 60 years. 3. On CT, both gland were shown in 135 (90.0%), the right in 143(95.3%), the left in142(94.6%). 4. In the shape of adrenal glands, most of right adrenal gland was linear or comet shaped; 68(47.6%),most of left adrenal gland was inverted-Y shaped: 103(72.6%). 5. In the length of adrenal glands, theright was2.5+-0.77cm, the left was 2.9+-0.75cm. 6. In the width of adrenal glands, the right was 3.2+-0.74cm, the left was2.7+-0.57cm. 7. In the thickness of adrenal glands, the right was 0.5+-0.14cm, the left was 0.6+-0.16cm.
Adrenal Glands ; Female ; Humans ; Male ; Mass Screening ; Pathology ; Pheniramine ; Tomography, X-Ray Computed

Bladder tumors initially may be diagnosed by excretory urography. In this study, 60 excretory urogramsincluding 40 cases of biopsy proved blaadder tumor and 20 cases of cystoscopically normal bladder were analyzed.Five experienced readers reviewed five radiographs of each case. The results were as follows: 1. Male to remaleratio was 5.7:1, and the highest incidence of age distribution was between 60 and 69 year with 48%. 2. The tumorswere chiefly located in lateral wall of bladder. (67%) 3. Among 40 cases, pathologic diagnosis was as follows:Transitional cell ca. 39 cases, and squamous cell ca. lcase. 4. The I.V.P. finding showed 82% of normal upperurinary tract, 13% of hydronephrosis, 5% of non-visualization, and 58% of filling defect in bladder. 5. Originalinterpretation of urogram was as follows: Of 40 cases with bladder ca., 45% as positive, 13% as suspicious, and38% as negative. Of 20 cases with normal, 95% as negative, and 5% as positive. 6. Analysis by this study was asfollows: Accuracy 68%, sensitivity 62%, specificity 80%, false positive rate 38%, and false negative rate 20%. 7.25 min A-P radiograph was most valuable projection and oblique projection did not significantly increasesensitivity.
Age Distribution ; Biopsy ; Diagnosis ; Epithelial Cells ; Humans ; Hydronephrosis ; Incidence ; Male ; Sensitivity and Specificity ; Urinary Bladder Neoplasms ; Urinary Bladder ; Urography

BACKGROUND: Adolescence offers unique opportunities for investment in health and well-being. The purpose of this study was to examine the common physical symptoms and diseases and medical utilization rate and the prevalence of health behaviors among school-aged adolescents. METHODS: This study was carried in 1998. Subjects consisted of 3,771 adolescents (ages 10 to 18) who attended an elementary, middle, and high school in Seoul and 3,246 parents. Self-report questionnaire was administered to the students and their parents to measure self-reported physical and psycho\social problems, practice of health behavior, medical care utilization rate, and the most common diagnosis. RESULTS: 1) Academic performance was the most common psycho\social problem for adolescents and their parents. 2) The perceived health status was highest in middle school students, lowest in female high school students. 3) As adolescents went to higher grades, the medical institute and dental clinic utilization rate tended to be decreased, and oriental clinic utilization rate did not change. The most common diagnosis during the past year was dental caries. The next most common diagnosis was respiratory infection. Accidents (males), headache (females) were more common in high school than middle school. 4) The most frequently reported symptom was fatigue in middle and high school students. More than half of female high school students reported fatigue, dizziness, headache, dysmenorrhea, hypersomnia during the last month. 5) The higher their grade was, the less adolescents practiced health behaviors. 21.9% of males and 1.9% of female high school students were smokers. 34.5% of males and 13.3% of female high school students drank alcohol on a regular basis. The prevalence of obesity was 12.0% in males, 10.0% in female high school students. Male adolescents tended to deal with stress with leisure activities, female adolescents did by talking about their problems with related people. CONCLUSION: This overview of health associated issues among adolescents provided a unique view of differences in health care needs between younger and older teens and between male and female teens. These data have meaningful implications for health promotion program for adolescents.
Adolescent* ; Delivery of Health Care ; Dental Caries ; Dental Clinics ; Diagnosis ; Disorders of Excessive Somnolence ; Dizziness ; Dysmenorrhea ; Fatigue ; Female ; Headache ; Health Behavior ; Health Promotion ; Humans ; Investments ; Leisure Activities ; Male ; Obesity ; Parents ; Prevalence ; Seoul* ; Surveys and Questionnaires

We describe two cases of primary thyroid lymphoma, involving 64-and 74-year-old women who presented with arapidly growing palpable mass in the anterior neck. In both patients, plain radiographs of this region re-vealedtracheal displacement and soft tissue mass, and CT scans demonstrated the presence of a large soft tis-sue masswith homogeneously low attenuation, predominantly located in the unilateral lobe of the thyroid g-land. Within themasses there was no calcification, necrosis, or hemorrhage, though in one patient the trachea was involvedposteriorly by the mass Both masses appeared as cold nodules on RI scan, and in both patients, the final diagnosiswas diffuse large cell type non-Hodgkin's lymphoma with a background of Hashimoto's thyroiditis.
Aged ; Female ; Hemorrhage ; Humans ; Lymphoma* ; Lymphoma, Non-Hodgkin ; Neck ; Necrosis ; Thyroid Gland ; Thyroiditis ; Tomography, X-Ray Computed ; Trachea

Background: Shift work is associated with obesity and metabolic syndrome. However, this association in the normal-weight population remains unclear. This study aimed to investigate whether shift work is associated with normal-weight obesity (NWO). Methods: From the nationally representative Korea National Health and Nutrition Examination Survey (KNHANES) dataset (2008 to 2011), 3,800 full-time workers aged ≥19 years with a body mass index (BMI) ≤25 kg/m2 were analysed. We defined NWO as BMI ≤25 kg/m2 and body fat percentage ≥25% in men and ≥37% in women. Working patterns were classified into “daytime,” “other than daytime,” and “shift.” Multivariable logistic regression analysis was performed to evaluate the relationship between shift work and NWO. Results: Shift work was associated with higher odds of NWO than daytime work (adjusted odds ratio [aOR], 1.47; 95% confidence interval [CI], 1.04 to 2.09) and night/evening work (aOR, 1.87; 95% CI, 1.11 to 3.14) after adjustment for type of work, working hours, age, sex, BMI, 25-hydroxyvitamin D levels, homeostatic model assessment for insulin resistance, and other sociodemographic factors. In subgroup analyses, the association between shift work and NWO was more robust in those aged ≥60 years and those working ≥56 hours/week. Conclusion Shift work was associated with NWO in community-dwelling Korean adults, independent of age, sex, BMI, and other covariates.

In this article, the statement of ethical statement about animal experiment was omitted.