1.Decreased Expression of 15-hydroxyprostaglandin Dehydrogenase in Gastric Carcinomas.
Tae Jung JANG ; Ye Seob JI ; Ki Hoon JUNG
Yonsei Medical Journal 2008;49(6):917-922
PURPOSE: Gastric carcinoma tissues release high level of prostaglandin E2 (PGE2) when compared to non-neoplastic mucosa, and cyclooxygenase-2 (COX-2), which is the rate-limiting enzyme in prostaglandin (PG) biosynthesis, is often overexpressed in gastric carcinomas and during gastric carcinogenesis. However, little is known about the expression of 15-hydroxyprostaglandin dehydrogenase (15-PGDH), the key enzyme responsible for the biological inactivation of PG, in gastric carcinomas. MATERIALS AND METHODS: We investigated the expression of 15-PGDH in 28 cases of advanced gastric carcinomas by Western blot analysis and also the relation between its expression and the gene promoter methylation. RESULTS: 15-PGDH expression was significantly decreased in gastric carcinomas compared to corresponding non-neoplastic tissues and inversely correlated with the expression of proliferating cell nuclear antigen in gastric carcinomas. However, there was no correlation between 15-PGDH expression and pathological findings such as nodal metastasis and vascular invasion. Promoter hypermethylation of 15-PGDH gene was not detected in carcinomas, with only a negligible expression of the enzyme. CONCLUSION: Our results suggested that 15-PGDH has tumor suppressor activity in gastric carcinomas.
Aged
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Base Sequence
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DNA Methylation
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DNA Primers/genetics
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DNA, Neoplasm/genetics
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Female
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Humans
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Hydroxyprostaglandin Dehydrogenases/genetics/*metabolism
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Male
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Middle Aged
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Promoter Regions, Genetic
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Stomach Neoplasms/*enzymology/genetics
2.15-Hydroxyprostaglandin Dehydrogenase in Colorectal Mucosa as a Potential Biomarker for Predicting Colorectal Neoplasms.
Hyo Jeong LEE ; Dong Hoon YANG ; Yeon Mi RYU ; Miyeoun SONG ; Ho June SONG ; Kee Wook JUNG ; Kyung Jo KIM ; Byong Duk YE ; Jeong Sik BYEON ; Eun Kyung CHOI ; Suk Kyun YANG ; Jin Ho KIM ; Seung Jae MYUNG
Journal of Korean Medical Science 2013;28(8):1154-1160
15-Hydroxyprostaglandin dehydrogenase (15-PGDH) is downregulated during the early stages of colorectal carcinogenesis. The aim of the present study was to investigate the potential role of 15-PGDH in normal-appearing colorectal mucosa as a biomarker for predicting colorectal neoplasms. We obtained paired tumor and normal tissues from the surgical specimens of 32 sporadic colorectal cancer patients. mRNA expression of 15-PGDH was measured using a quantitative real-time PCR assay. We evaluated the association between 15-PGDH mRNA expression in normal-appearing mucosa, the presence of synchronous adenoma, and the cumulative incidence of metachronous adenoma. The relative 15-PGDH expression of normal-appearing mucosa in patients with synchronous adenoma was significantly lower than in patients without synchronous adenoma (0.71 vs 1.00, P = 0.044). The patients in the lowest tertile of 15-PGDH expression in normal-appearing mucosa were most likely to have synchronous adenoma (OR: 10.5, P = 0.024). Patients with low 15-PGDH expression in normal-appearing mucosa also demonstrated more advanced stage colorectal cancer (P = 0.045). However, there was no significant difference in the cumulative incidence of metachronous adenoma according to 15-PGDH mRNA expression in normal-appearing mucosa (P = 0.333). Hence, 15-PGDH in normal-appearing colorectal mucosa can be a useful biomarker of field effect for the prediction of sporadic synchronous neoplasms.
Aged
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Colorectal Neoplasms/*diagnosis/enzymology/pathology
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Down-Regulation
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Female
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Humans
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Hydroxyprostaglandin Dehydrogenases/genetics/*metabolism
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Intestinal Mucosa/*enzymology
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Male
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Middle Aged
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Neoplasm Staging
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Neoplasms, Multiple Primary/enzymology/pathology
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Neoplasms, Second Primary/enzymology/pathology
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Odds Ratio
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Predictive Value of Tests
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RNA, Messenger/metabolism
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Real-Time Polymerase Chain Reaction
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Risk Factors
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Tumor Markers, Biological/*metabolism