Animals ; Contrast Media ; Dogs ; Dose-Response Relationship, Drug ; Echocardiography ; Haplorhini ; Hydrogen Peroxide ; administration & dosage

To study plasma hemoglobin cats were given serial enemas of 0.5 and 0.25% H2O2 with human who1e blood, single enemas of 0.5% H2O2 and single intravenous administration of 0.5% H2O2. Plasma hemoglobin levels were abnormally high in both the serial enema group and the intravenous group, while the plasma hemoglobin level was within normal range in the single enema group. Therefore a single enema of 0.5% H2O2 with human whole blood can be utilized with safety clinically for 60 to 75 minutes to relieve hypoxia.
Animal ; Anoxia/drug therapy* ; Blood* ; Cats ; Comparative Study ; Enema ; Female ; Hemoglobins/analysis* ; Hydrogen Peroxide/administration & dosage* ; Hydrogen Peroxide/therapeutic use ; Injections, Intravenous ; Male

OBJECTIVETo investigate how transient low dose of hydroperoxide pretreatment prevents cardiac ischemia/reperfusion injury.

METHODSSD rats were divided into 4 groups: sham operation (Sham), standard ischemia/reperfusion (I/R), ischemic preconditioning (IPC) and IR preceded by low H2O2 treatment. Cardiac function and injury parameter were compared among groups.

RESULTSIPC protected reperfusion injury and improved cardiac function. Low H2O2 treatment played a role in cardioprotection similar to IPC. Low H2O2 was indeed generated in the early phase of simulated ischemia and attenuated cytochrome c release induced by high Ca2+ in isolated mitochondria.

CONCLUSIONLow H2O2 plays a critical role in cardioprotection probably by inhibiting mitochondrial permeability transition.

Animals ; Hydrogen Peroxide ; administration & dosage ; Ischemic Preconditioning ; methods ; Male ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury ; prevention & control

Background: Reactive oxygen species (ROS) generated by neutrophils are closely correlated with the pathogenesis of a variety of inflammatory skin diseases. The aim of this study was to investigate whether the amount of reactive oxygen species (hydrogen peroxide) generated by neutrophils from patients with acne inflammation decrease after oral administration of standard doses of isotretinoin. Method: In order to measure neutrophil hydrogen peroxide production, phorbol myristate acetate (PMA, neutrophil stimulant), was added to whole blood. Intracellular dichlorofluorescein (DCF) fluorescence of neutrophils was determined by flow cytometry. In order to assess treatment efficacy, we used a Global Acne Grading Score (GAGS) and assessed the efficacy based on examinations at baseline and week 8. Results: Patients with acne inflammation showed a significantly increased level of hydrogen peroxide produced by neutrophils compared to healthy controls. Patients with acne inflammation treated with isotretinoin showed a significant decrease in the ability of neutrophils to produce hydrogen peroxide in accordance with a clinical improvement of acne lesions. Conclusion: Our result shows that the generation of ROS which induce a chemical insult to the integrity of the follicular epithelium in acne, can be suppressed in isotretinoin-treated acne patients.
Acne Vulgaris* ; Administration, Oral ; Epithelium ; Flow Cytometry ; Fluorescence ; Humans ; Hydrogen Peroxide* ; Hydrogen* ; Inflammation ; Isotretinoin* ; Neutrophils* ; Reactive Oxygen Species ; Skin Diseases ; Tetradecanoylphorbol Acetate ; Treatment Outcome

An investigation of extrapulmonary oxygenation was made in dogs, rabbits and, finally, in a case of Tetralogy of Fallot using an intestinal perfusion of hydrogen peroxide (H2O2). For a single administration, 0.4 per cent H2O2 can be given safely by enema, in doses of 10ml./Kg. of body weight, this would give maximum oxygenation in both the portal vein and inferior vena cava without the formation of gas emboli. Concentrations higher than this caused gas bubbles in the portal vein. For serial administrations, 0.2 per cent H2O2 can be given by enema exchanging the intestinal contents at 10 to 15 minutes intervals. When given concomitantly with human whole blood, 1.0ml./Kg. of body weight, there is a prolonged higher oxygenation in the portal vein, inferior vena cava and femoral artery. This concentation of H2O2 would not cause gas emboli in the portal vein. Although extrapulmonary oxygenation is possible by giving oxygen by enema, this method would cause too much abdominal distension. In experiments of death by suffocation, the group given H2O2 had doubled the duration of E.K.G. activity when compared with controls. One patient with Tetralogy of Fallot, confirmed by clinical findings, X-ray studies, E.K.G. and cardiac catheterization, who was not suitable for cardiac surgery because of low mentality, was selected for this study. 0.2 per cent H2O2, 10ml. per Kg. of body weight by enema, exchanging intestinal contents at 30 minutes intervals, resulted in a marked elevation of the pO2 in the venous blood and in the inferior vena cava. There was a disappearance of finger tip and toe tip cyanosis and flushing of the soles and palms was noted during the procedure.
Acidosis, Respiratory/diagnosis ; Animals ; Asphyxia/therapy ; Carbon Dioxide/blood ; Child ; Dogs ; Enema ; Female ; Hematocrit ; Human ; Hydrogen Peroxide/administration & dosage/*therapeutic use ; Male ; Oxygen/blood ; Tetralogy of Fallot/*therapy

OBJECTIVETo investigate the H2O2-induced expression of human histone acetyltransferase-like protein (hALP), a telomerase regulation-associated gene, and its effects on the stress-triggered cellular senescence.

METHODSThe induced expression of hALP was measured by semi-quantitative RT-PCR and immunofluorescent histochemistry after treatment of HeLa cells by H2O2. The effects of hALP expression on cellular responses to H2O2 were analyzed by MTT, flowcytometry, and SA-beta-gal staining, respectively.

RESULTShALP mRNA could be dose-dependently induced by treatments of 0.2-1.6 mmol/L H2O2, and the induction could be observed after 6 hours and kept for 36 hours in the presence of 0.4 mmol/L H2O2. Meanwhile, the immunofluorescent staining showed marked stronger nuclear intensity of hALP protein in H2O2-treated HeLa cells. In the treatment of H2O2, the ectopic expression of hALP enhanced continuous growth and overcame G2/M arrest as well as decreased senescence-associated beta-gal staining. On the contrary, the transfected clones with antisense or blank vector and original He-La cells presented growth suppression, G2/M delay and higher percentage of SA-beta-gal activities in the presence of H2O2.

CONCLUSIONSThe expression of hALP could be up-regulated by treatment of H2O2, and elevated expression could enhance cellular resistance to H2O2-induced cellular senescence. The data might be of references to elucidation of basic biological function of hALP gene and its associated telomerase activity.

Asparaginase ; biosynthesis ; genetics ; Autoantigens ; biosynthesis ; genetics ; Cell Cycle ; Cell Proliferation ; Cellular Senescence ; drug effects ; Dose-Response Relationship, Drug ; HeLa Cells ; Humans ; Hydrogen Peroxide ; administration & dosage ; pharmacology ; Oxidants ; administration & dosage ; pharmacology ; RNA, Messenger ; biosynthesis ; genetics ; Transfection ; Up-Regulation

OBJECTIVETo study of exogenous substances on the germination rate of Platycodon grandiflorum and offer the basis for the standardized culture of P. grandiflorum.

METHODAt 25 degrees C, under darkness and lightness, do pretreatmeats on seeds by using GA3, H2O2, KMnO4, PEG at different concentrations.

RESULTThe results indicated that the pretreatments with GA3 at a concentrating of 50-250 mg x L(-1) and 1%-2% of H2O2 could increase germination of P. grandiflorum seed at a certain degree. Contrariwise 0.1%-0.5% of KMnO4 and 15%-35% of PEG inhibited the germination.

CONCLUSIONPretreatment with 50-250 mg x L(-1)GA3 and H2O2 at a low cencontration could increase the seed germination of P. grandiflorum.

Dose-Response Relationship, Drug ; Germination ; physiology ; Gibberellins ; administration & dosage ; pharmacology ; Hydrogen Peroxide ; administration & dosage ; pharmacology ; Light ; Plant Growth Regulators ; pharmacology ; Plants, Medicinal ; growth & development ; physiology ; Platycodon ; growth & development ; physiology ; Polyethylene Glycols ; pharmacology ; Potassium Permanganate ; pharmacology ; Seeds ; growth & development ; physiology

PURPOSE: Chemical lights, also called Luminous Sticks, consist of a solution of diphenyl oxalate (C14H10O4) and hydrogen peroxide (H2O2). Human tissue can be damaged when the mixed solution contacts the human body. The authors report a single case of chemical injury of keratoconjunctiva by exposure to chemical lights. CASE SUMMARY: A 47-year-old man's right eye accidentally contacted the fluorescent material when breaking a Luminous Stick 7 days before being referred to our clinic. He had pain in the right eye and experienced visual loss. The patient's best corrected visual acuity in the right eye was 20/50. An ulcerative lesion with edema at the inferior bulbar and palpebral conjunctiva and coneal epithelial defect was observed upon biomicroscopic examination. The patient was hospitalized and antibiotics, steroids, mydriatic and artificial tear eye drops were applied for treatment. After 9 days of treatment, the best corrected visual acuity of the patient recovered to 20/20, and the conjunctiva and cornea were mostly healed. No complication was observed. CONCLUSIONS: Chemical lights are commonly used in concerts and festivals. If the contents contact the eyes when breaking he chemical lights, various chemical burns can occur and cause ophthalmologic complications. Since no regulations have been passed regarding chemical lights, safety education and supervision are considered to be necessary for children.
Anti-Bacterial Agents ; Biphenyl Compounds ; Burns, Chemical ; Child ; Conjunctiva ; Cornea ; Edema ; Eye ; Holidays ; Human Body ; Humans ; Hydrogen Peroxide ; Light ; Middle Aged ; Ophthalmic Solutions ; Organization and Administration ; Social Control, Formal ; Steroids ; Tears ; Ulcer ; Visual Acuity

To study the effect of Cu2+ and Zn2+ on amyloid-beta peptides (Abeta) aggregation, the morphology, size and cell toxicity of Abeta40 aggregates formed with the metal ions have been observed by the methods including ultraviolet spectroscopy, fluorescence spectroscopy and transmission electron microscopy. The results showed that Cu2+ and Zn2+ can accelerate Abeta40 aggregation, and both changed the morphology and size of Abeta40 aggregates. Zn2+ induced Abeta40 to form fibrous Abeta40 aggregates, while the amorphous and fibrous aggregates were produced by the interaction between Cu2+ and Abeta40. In addition, H2O2 was produced when Abeta40 reduced Cu2+. The relationship between metal ions and Abeta40 aggregates was analyzed, and the function of metal ions in Alzheimer's disease (AD) was illustrated in the research.
Amyloid beta-Peptides ; chemistry ; Cell Survival ; drug effects ; Copper ; administration & dosage ; chemistry ; toxicity ; Dose-Response Relationship, Drug ; HeLa Cells ; Humans ; Hydrogen Peroxide ; chemistry ; Ions ; chemistry ; Microscopy, Electron, Transmission ; Peptide Fragments ; chemistry ; Spectrometry, Fluorescence ; Spectrophotometry, Ultraviolet ; Zinc ; chemistry

OBJECTIVETo confirm the responses and function of hALP, a telomerase-regulation associated gene, in DNA damage.

METHODSHeLa and Hep2 cells were treated by genotoxic agents H2O2 and cisplatin, and the induced expression of hALP was measured by quantitative RT-PCR and immunofluorescent histochemistry. The alterations in transcriptional activity of hALP promoter were estimated by luciferase reporter assays. The effects of genotoxic agents on cells in different status of hALP expression were analyzed by MTT method.

RESULTSThe level of hALP mRNA could be increased when treated by 0.2 - 1.6 mmol/L H2O2 and reach a peak in concentration of 0.4 mmol/L. The induction could be observed after 6 h in the treatment of 0.4 mmol/L H2O2 and the higher level can be retained for 36 h. Similarly, cisplatin induced hALP mRNA expression is also dose and time dependent. The immunofluorescent staining showed that the treatment of 0.2 or 0.4 mmol/L H2O2, 0.2 or 0.5 micromol/L cisplatin increased the intensity of hALP protein in cellular nuclei. The luciferase assays demonstrated that both H2O2 and cisplatin could up-regulate hALP promoter activity through its upstream - 705 - +20 nt region. In cell survivor assay, the HeLa cells expressing sense hALP gene could grow continuously in the presence of 0.4 mmol/L H2O2 or 0. 5 micromol/L cisplatin while cells with antisense hALP or control cells were slower in growth.

CONCLUSIONSThe expression of hALP gene could be up-regulated by DNA damage through activating transcription of its promoter, and increase cellular resistance to genotoxic agents.

Antineoplastic Agents ; administration & dosage ; pharmacology ; Asparaginase ; biosynthesis ; genetics ; Autoantigens ; biosynthesis ; genetics ; Cell Proliferation ; Cells, Cultured ; Cisplatin ; administration & dosage ; pharmacology ; DNA Damage ; physiology ; Dose-Response Relationship, Drug ; Gene Expression Regulation, Enzymologic ; HeLa Cells ; metabolism ; Humans ; Hydrogen Peroxide ; administration & dosage ; pharmacology ; RNA, Messenger ; biosynthesis ; genetics ; Telomerase ; genetics ; physiology ; Up-Regulation