1.Production and characterization of human T cell hybridomas.
Eung Soo HWANG ; Dong Gyun LIM ; Ae Young LEE ; Ju Young SEOH ; Chung Gyu PARK ; Yoon Hoh KOOK ; Myung Sik CHOI ; Chang Yong CHA
Journal of the Korean Society for Microbiology 1992;27(6):571-577
No abstract available.
Humans*
;
Hybridomas*
2.Regulation of cellular immunity in cryptococcosis III. induction ofcryptococcus neoformans-specific T cells and hybridoma.
Tai You HA ; Young Min PARK ; Yoon Kyu PARK ; Hern Ku LEE ; Chong Wook PARK
Korean Journal of Immunology 1992;14(1):15-23
No abstract available.
Cryptococcosis*
;
Hybridomas*
;
Immunity, Cellular*
;
T-Lymphocytes*
3.Regulation of cellular immunity in cryptococcosis III. induction ofcryptococcus neoformans-specific T cells and hybridoma.
Tai You HA ; Young Min PARK ; Yoon Kyu PARK ; Hern Ku LEE ; Chong Wook PARK
Korean Journal of Immunology 1992;14(1):15-23
No abstract available.
Cryptococcosis*
;
Hybridomas*
;
Immunity, Cellular*
;
T-Lymphocytes*
4.Advances in antibody drug expression techniques.
Mengxiao ZHANG ; Jianwei ZHU ; Huili LU
Chinese Journal of Biotechnology 2019;35(2):171-182
The 21st century is regarded as the century of biotechnological drugs, among which monoclonal antibodies and their derived targeting drugs have established themselves as the leading modality of biopharmaceutical pharmaceutics for a wide range of indications covering malignant tumors and autoimmune disorders. Since the manufacturing of the first antibody drug from hybridoma cells, the technologies have been intensely studied and there emerged numerous breakthroughs in recombinant cell line establishment, antibody expression and purification, quality control and other related areas. This article summarizes the critical progresses of antibody drugs expression technologies, especially of mammalian cell expression system, Escherichia coli expression system, the transgenic animal reactor and the cell free protein synthesis system, to give a detailed illustration of the recent advances in antibody drugs development.
Animals
;
Antibodies, Monoclonal
;
Biotechnology
;
Cell Line
;
Escherichia coli
;
Hybridomas
5.Research progress in the rapid preparation of monoclonal antibodies of mouse hybridoma.
Shuiqin FANG ; Cheng LIU ; Junfei MA ; Shuaishuai YAN ; Dongpo XU ; Qing LIU
Chinese Journal of Biotechnology 2021;37(7):2293-2306
Mouse hybridoma monoclonal antibody is the most commonly used antibody in immunology because of its stable source, easy preparation in later stage and high yield. The traditional time-consuming and laborious hybridoma preparation technology could not meet the growing market demand. In this paper, we describe the rapid preparation techniques involved in antigen design and screening, B cell enrichment and screening, transgenic myeloma cells, fusion technology improvement, positive hybridoma cell screening and rapid detection of monoclonal antibody performance, to provide a reference for rapid preparation of mouse hybridoma monoclonal antibody.
Animals
;
Antibodies, Monoclonal
;
Antigens
;
B-Lymphocytes
;
Hybridomas
;
Mice
6.Effect of Anti - idiotype Antibody on Anti - DNA Antibody Production by Hybridoma Cells.
Jung Koo YOUN ; Hyung Il KIM ; Joo Deuk KIM ; Sun PARK ; Young Tai KIM ; Milli Na LEE
Korean Journal of Immunology 1997;19(4):513-524
Anti-idiotype antibody (anti-id Ab) which recognizes idiotope in the variable region of immunoglobulin (Ig) can regulate Ab production by B cells in vivo and in vitro. Although it has been reported that anti-id Ab can suppress IgM production by lymphocytes or hybridoma cells without suppression of cell proliferation, the regulatory mechanism of anti-id Ab is not completely understood. We studied the effects of anti-id Ab on the production of IgG class anti-DNA Ab by hybridoma cells, on the proliferation of cells, and on the transcription levels of Ig genes. In contrast to suppressive effect of anti-id Ab on the production of IgM previously reported by others, stimulatory effects of anti-id Ab on the production of IgG by hybridoma cells as well as the proliferation of these .cells were observed. However, little effect of anti-id Ab on the transcription levels of Ig genes was observed. These results suggest that anti-id Ab can increase Ab production by stimulation of cell proliferation. Furthermore, these results suggest that the effect of anti-id Ab on the production of Ab may be determined by the difference in class of Ab produced by hybridoma cells following the treatment with anti-id Ab.
Antibody Formation*
;
B-Lymphocytes
;
Cell Proliferation
;
DNA*
;
Genes, Immunoglobulin
;
Hybridomas*
;
Immunoglobulin G
;
Immunoglobulin M
;
Immunoglobulins
;
Lymphocytes
7.C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches.
Kana TOZAKI ; Junpei KIMURA ; Masahiro YASUDA ; Nobuyuki RYU ; Testuo NASU ; Anton PERNTHANER ; Wayne R HEIN
Journal of Veterinary Science 2013;14(1):1-6
The follicle-associated epithelium (FAE) of Peyer's patches (PPs) contains M cells that are important for reducing mucosal immune responses by transporting antigens into the underlying lymphoid tissue. We generated a monoclonal antibody (C6) that reacted with the FAE of calf ileal PPs, and analyzed the characteristics of C6 using immunohistochemistry and Western blotting. FAE of the ileal PP was stained with C6 during both late fetal developmental and postnatal stages. Neither the villous epithelial cell nor intestinal crypt basal cells were stained at any developmental stage. During the prenatal stages, FAE of the jejunal PP was C6-negative. However, a few C6-positive cells were distributed diffusely in some FAE of the jejunal PPs during the postnatal stages. The protein molecular weight of the antigen recognized by C6 was approximately 45 kDa. These data show that C6 is useful for identifying the FAE in ileal PPs and further suggest that differentiation of the FAE in these areas is independent of external antigens.
Animals
;
Antibodies, Monoclonal/*immunology
;
*Cattle
;
Fetus
;
Hybridomas
;
Ileum/*ultrastructure
;
Intestinal Mucosa/*immunology
;
Peyer's Patches/*immunology/ultrastructure
8.C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches.
Kana TOZAKI ; Junpei KIMURA ; Masahiro YASUDA ; Nobuyuki RYU ; Testuo NASU ; Anton PERNTHANER ; Wayne R HEIN
Journal of Veterinary Science 2013;14(1):1-6
The follicle-associated epithelium (FAE) of Peyer's patches (PPs) contains M cells that are important for reducing mucosal immune responses by transporting antigens into the underlying lymphoid tissue. We generated a monoclonal antibody (C6) that reacted with the FAE of calf ileal PPs, and analyzed the characteristics of C6 using immunohistochemistry and Western blotting. FAE of the ileal PP was stained with C6 during both late fetal developmental and postnatal stages. Neither the villous epithelial cell nor intestinal crypt basal cells were stained at any developmental stage. During the prenatal stages, FAE of the jejunal PP was C6-negative. However, a few C6-positive cells were distributed diffusely in some FAE of the jejunal PPs during the postnatal stages. The protein molecular weight of the antigen recognized by C6 was approximately 45 kDa. These data show that C6 is useful for identifying the FAE in ileal PPs and further suggest that differentiation of the FAE in these areas is independent of external antigens.
Animals
;
Antibodies, Monoclonal/*immunology
;
*Cattle
;
Fetus
;
Hybridomas
;
Ileum/*ultrastructure
;
Intestinal Mucosa/*immunology
;
Peyer's Patches/*immunology/ultrastructure
9.Progress in preparation of small monoclonal antibodies of knock out technique.
Jing LIU ; Xin-min MAO ; Lin-lin LI ; Xin-xia LI ; Ye WANG ; Yi LAN
China Journal of Chinese Materia Medica 2015;40(19):3737-3741
With the application of monoclonal antibody technology more and more widely, its production technology is becoming more and more perfect. Small molecule monoclonal antibody technology is becoming a hot research topic for people. The application of traditional Chinese medicine small molecule monoclonal antibody technology has been more and more widely, the technology for effective Chinese medicine component knockout provide strong technical support. The preparation of monoclonal antibodies and small molecule knockout technology are reviewed in this paper. The preparation of several steps, such as: in the process of preparation of antigen, hapten carrier coupling, coupling ratio determination and identification of artificial antigen and establishment of animal immunization and hybridoma cell lines of monoclonal antibody, the large-scale preparation; small molecule monoclonal antibody on Immune in affinity chromatography column method is discussed in detail. The author believes that this technology will make the traditional Chinese medicine research on a higher level, and improve the level of internationalization of Chinese medicine research.
Animals
;
Antibodies, Monoclonal
;
chemistry
;
genetics
;
immunology
;
Humans
;
Hybridomas
;
metabolism
;
Immunologic Techniques
;
methods
;
trends
10.Recovery and purification of monoclonal antibody producted by continuous perfusion culture using expended bed adsorption.
Xiao-Ling YU ; Li MI ; Xi-Ying YAO ; Zhi-Nan CHEN
Chinese Journal of Biotechnology 2004;20(2):227-232
Monoclonal antibody producted by continuous perfusion culture was recovered and purified by expended bed adsorption chromatography. A packed bed column XK16/20 was used for method scouting with Streamline SP adsorbent. Two expended bed columns Streamline-25 and -50 were used for method optimization and pilot scale experiment, respectively. The recovery yield of monoclonal antibody was above 90% in a 5 - 7 fold enhanced purity and 10 fold increased concentration. According to the different concentration of monoclonal antibody in cell culture broth, about 18 - 50L fluid can be treated in a single cycle. MAb purification from lab scale (400mg per cycle) to a small pilot scale (2g per cycle) has been achieved. Compared with packed bed adsorption, the preparation cycle was half shortened, and the cost of production and the complexity of process were decreased markedly. It has been proven that a purification process based on expended bed adsorption technique is simple, efficient and economical.
Adsorption
;
Antibodies, Monoclonal
;
biosynthesis
;
isolation & purification
;
Bioreactors
;
Cell Culture Techniques
;
methods
;
Chromatography
;
methods
;
Hybridomas
;
cytology
;
metabolism