1.Detection of leptoapiral DNA by nucleic acid hybridization with 32P-labeld probe.
Seok Yong KIM ; Woo Hyun CHANG ; Ik Sang KIM
Journal of the Korean Society for Microbiology 1991;26(4):329-335
No abstract available.
DNA*
;
Nucleic Acid Hybridization*
2.RAN-aCGH: R GUI Tools for Analysis and Visualization of an Array-CGH Experiment.
Genomics & Informatics 2007;5(3):137-139
RAN-aCGH is an R GUI tool for the analysis and visualization of array comparative genomic hybridization (array-CGH) experiments. The tool consists of data-loading, preprocessing for missing data, several methods for statistical identification of DNA copy number aberration, and visualization of the copy number change. RAN-aCGH requires a single input format, provides various visualizations, and allows the addition of a new statistical method, all in a user-friendly graphic user interface (GUI).
Comparative Genomic Hybridization
;
DNA
3.Detection of herpesviruses in renal allograft rejection by in situ hybridization.
Wan Shik SHIN ; In Sik PARK ; Yoon Sik CHANG ; Yung Jin CHOI ; Sang In SHIM ; Jin Han KANG ; Jin Hong YOO ; Yang Lee KIM ; Yung Suk YOON ; Moon Won KANG ; Byung Kee BANG
Korean Journal of Infectious Diseases 1993;25(4):351-355
No abstract available.
Allografts*
;
Herpesviridae*
;
In Situ Hybridization*
4.Easy Application of Digoxigenin-11-dUTP Labelled Probe in Detection of Human Papillomavirus DNA.
Annals of Dermatology 1991;3(2):91-95
In situ hybridization was performed in ten cases of condyloma acuminata in order to study the applicability of digoxigenin-11 dUTP(Dig-dUTP) labelled probe compared with radioactive isotope labelled probes. Although signal intensity was denser in radiolabelled probes, high positive rates were obtained with Dig-dUTP labelled probes. From these results, Dig-dUTP labelling is found to be more efficient in typing of human papillomavirus DNA than radiolabelling.
DNA*
;
Humans*
;
In Situ Hybridization
5.Easy Application of Digoxigenin-11-dUTP Labelled Probe in Detection of Human Papillomavirus DNA.
Annals of Dermatology 1991;3(2):91-95
In situ hybridization was performed in ten cases of condyloma acuminata in order to study the applicability of digoxigenin-11 dUTP(Dig-dUTP) labelled probe compared with radioactive isotope labelled probes. Although signal intensity was denser in radiolabelled probes, high positive rates were obtained with Dig-dUTP labelled probes. From these results, Dig-dUTP labelling is found to be more efficient in typing of human papillomavirus DNA than radiolabelling.
DNA*
;
Humans*
;
In Situ Hybridization
6.Advantages of Array Comparative Genomic Hybridization Using Buccal Swab DNA for Detecting Pallister-Killian Syndrome
Veronica BERTINI ; Simone GANA ; Alessandro ORSINI ; Alice BONUCCELLI ; Diego PERONI ; Valetto ANGELO
Annals of Laboratory Medicine 2019;39(2):232-234
No abstract available.
Comparative Genomic Hybridization
;
DNA
7.Characterizing Atypical BCL6 Signal Patterns Detected by Digital Fluorescence In Situ Hybridization (FISH) Analysis.
Michael LIEW ; Leslie R ROWE ; Phillipe SZANKASI ; Christian N PAXTON ; Todd KELLEY ; Reha M TOYDEMIR ; Mohamed E SALAMA
Annals of Laboratory Medicine 2018;38(6):619-622
No abstract available.
Fluorescence*
;
In Situ Hybridization*
9.Molecular biomarkers detected using fluorescence in situ hybridizationin a Filipino with retinoblastoma
Arnold Dominic A. Barzaga ; Glenmarie Angelica S. Perias ; Lia Angela E. Reyes ; Patrick Gabriel G. Moreno ; Patrick R. Relacion ; Richelle Ann M. Manalo ; Yasmyne C. Ronquillo ; Francisco M. Heralde III
Acta Medica Philippina 2024;58(10):99-107
Background and Objective:
Retinoblastoma is one of the most common intraocular cancers among children usually caused by the loss of retinoblastoma protein function. Despite being a highly heritable disease, conventional diagnostic and prognostic methods depend on clinical examination, with limited consideration of cancer genetics in the standard of care. CD133, KRT19, and MUC1 are commonly explored genes for their utility in liquid biopsies of cancer including lung adenocarcinoma. To date, there are few extensive molecular studies on retinoblastoma in Filipino patients. To this end, the study aimed to describe the copy number of CD133, KRT19, and MUC1 in retinoblastoma samples from a Filipino patient and quantitate the respective expression level of these genes.
Methods:
Hematoxylin & Eosin (H&E) staining was utilized to characterize the retinoblastoma tissue while fluorescence in situ hybridization (FISH) using probes specific to CD133, KRT19, and MUC1 was performed to determine the copy number of genes in retinoblastoma samples from a Filipino patient (n = 1). The gene expression of CD133, MUC1, and KRT19 was quantitated using RT-qPCR.
Results:
The H&E staining in the retinoblastoma tissue shows poorly differentiated cells with prominent basophilic nuclei. CD133 was approximately 1.5-fold overexpressed in the retinoblastoma tissue with respect to the normal tissue, while MUC1 and KRT19 are only slightly expressed. Multiple intense signals of each probe were localized in the same nuclear areas throughout the retinoblastoma tissue, with high background noise.
Conclusion
These findings suggest that CD133 is a potential biomarker for the staging and diagnosis of retinoblastoma in Filipino cancer patients. However, further optimization of the hybridization procedures is recommended.
Retinoblastoma
;
Biomarkers
;
In Situ Hybridization
10.Current Status and Future Clinical Applications of Array.based Comparative Genomic Hybridization.
Genomics & Informatics 2004;2(3):113-120
No abstract available.
Chromosome Aberrations
;
Comparative Genomic Hybridization*
Result Analysis
Print
Save
E-mail