Mouse fetuses beginning from 13-day plug age and new borns from 1-dayto the adult stage were prepared for the study of acid and alkaline phosphatasesaccording to MacDonald's and Danielli's modified methods of Gomeri respective-ly. It was found that the distribution of acid and alkaline phosphatases in thedevelopment of vibrissae is essentially the same. The enzymes begin to increasein the condensed mesenchymal anlage of papilla and in the invaginating epidermisof 14th day fetus. The phosphatases in the papilla and then in the connectivetissue sheath keep their high concentration from the beginning of hair follicleformation to the adult stage, while those in the epidermal sheath are graduallydecreased and localized in the course of differentiation. The bulb has higheramount of enzymes than the distal region of the follicle throughout all the stagesstudied. The significance of distribution of the enzymes is discussed.

Adult albino rats, weighing 140-380 grams, were chosen for study on the effects of starvation, anaesthesia and nervous stimulation on liver glyco- gen and ribonucleic acid. Tissues were fixed in cold Carnoy's fluid, embedded in paraffin, stained with McManus' PAS method for glycogen and with methyl green-pyronine method for ribonucleic acid. Glycogen decreased considerably after 5 to 6 hours starvation and disap- peared completely after 8 hours. Ribonucleic acid also showed signs of decomposition and became more diffusely distributed. Anaesthetic starvation after intraperitoneal injection of 1% sodium amytal had less effect than that of starvation. When sciatic nerves of old rats were stimulated with 0.7-0.9 volts inducted current for 4 to 6 hours, liver glycogen disappeared completely and the depletion of liver ribonucleic acid was more pronounced than that of starvation. With young rats of 2 to 3 months of age, the influence was indistinct.

In the course of investigation on the histochemical changes in the rat adrenal glandafter prolonged electrical stimulation of sciatic nerve, neurons were found in medulla.Their presence in the rat has been an unsettled problem in the literature. It order tobring out a better picture of the nerve cells, three more rats, all over 2 years old, werekilled for silver preparations including Cajals Ⅳ, Ⅵ, Ranson's and Willard's modifications.They were sectioned in series. The number of nerve cells and their way of groupingwere found to differ in different specimens, including those from the same individual.Occasionally nerve cells were located under the capsule and in the zona reticulosa. The innervation of adrenal cortex was another problem under investigation. Inthe Cajal's Ⅵ preparation the reticular fibers were not stained so that the nerve fibersand neurofibrils were clearly shown. From this preparation were found looped terminalboutons at the periphery of the cells in the zona reticulosa and zona fasciculata.

Under stimulation of electric current adult albino mice were made to run around on the wire without stop for 1 to 4 hours in a wooden case. In starved animals glycogen began to decrease 60 minutes after depri- vation of food and reached to about half within 2 to 3 1/2 hours. Six hours later only a few liver cells still contained some glycogen granules. Deple- tion of ribonueleic acid took place at a much slower rate. It broke from larger masses into tiny rods and filaments, sparsely distributed. No change of nucleus and nucleolus was noted. In exhausted animals changes of liver glycogen and ribonucleic acid were much more conspicuous than the starved ones. In most cases 2 to 3 1/2 hours' fatigue caused complete disappearance of glycogen granules and the reduc- tion of the ribonucleic acid. The chromatin condensed into larger knots or adhered to the inner side of the nuclear membrane. Central veins and sinusoids became enlarged and congested. Liver cells became smaller. Kupffer cells increased in number and their nuclei became darker.