Objective To develop a virtual world integration scale for adolescents and test its reliability and validity within the framework of the Virtual Social Ecology Model. Methods A total of 2543 students from four schools in Wuhan and Xianning were recruited from March to October 2023. The preliminary items of the scale were determined by semi-structured interview, literature review, brainstorming and Delphi expert consultation. The compiled scale was analyzed for validity and reliability using critical ration analysis, homogeneity test, confirmatory factor analysis, correlation related validity test , cronbach α coefficient, and split-half coefficient. Results The scale was preliminarily determined to consist of 20 items in 10 dimensions, including virtual self-identity, emotional interaction, virtual social interaction, cyber bullying, community activities, value identity, community participation, community management, network culture and virtual social capital. In the item analysis, the correlation coefficient of homogeneity test ranged from 0.496 to 0.767 (P<0.001), and there was statistical difference in critical ratio analysis (CR= 21.897-53.546, P<0.001). The fits of the confirmatory factor analysis model in validity analysis were: CFI=0.933, NFI=0.951, IFI=0.927, and RMSEA=0.064. The criterion validity showed a significantly positive association (the coefficient was between 0.450 and 0.855, P<0.01). The overall Cronbach α coefficient was 0.929 and the split-half coefficient was 0.846. The Cronbach α coefficients of sub-questionnaires were between 0.719 and 0.900 , and the split-half coefficients were between 0.729 and 0.913. Conclusion The Virtual World Integration Scale has good reliability and validity, and can be used as an assessment tool for the level of adolescents’ integration in virtual world.