Objective:To construct eukaryotic expression vectors by usingthep Silencer2.0 vector for inhibiting human livin gene by RNA interference,and to detect the effect of the silenced livin gene on the growth and apoptosis of U251 cells. Methods:Two target gene segments were synthesized and cloned into the pSilencer2.0 vectorrespectively to construct two recombinant eukaryotic expression vectors: pSilencer2.0-liv1 and pSilencer2.0-liv2,both of which were identified by enzyme digestion analysis. Then the U251 cells were transfected with the recombinant vectors and the interfering effects were detected by RT-PCR and Western blot. The proliferation of the U251 cells was detected by MTT method and their apoptosis index detected by flow cytometry. Results:The results of Enzyme digestion analysis showed that two target segments were cloned into pSilencer2.0 vectors. The results of RT-PCR and Western blot indicated that pSilencer2. 0-liv1 and pSilencer2.0-liv2 vectors could knock down the transcription and expression of livin gene,but the effect of pSilencer2.0-liv2 vectors was better than that of the pSilencer2.0-liv1 vectors. Obvious decrease in growth of the U251 cells was observed after transfected with pSilencer2.0-liv1 and pSilencer2.0-liv2 vectors,along with 17%increase of the apoptosis index in the U251 cells transfected with pSilencer2.0-liv2 vectors. Conclusion:The transcription and expression of livin gene were inhibited effectively in the glioma cell line U251. Aftertransfected with pSilencer2.0-liv2 vectors,the apoptosis index of the U251 cells was increased and the growth inhibited.

Objective To discuss the clinical results and surgical method in treatment of hypertensive intracerebral hemorrhage in basal ganglia. Methods From January 2007 to June 2013, the use of surgery treatment of hypertensive intracerebral hemorrhage in basal ganglia under direct vision assisted by the deep cold light suction in 104 cases,all the cases were done through lateral fissure-brain island approach,removing the hematoma with bipolar coagulation and hemostasis at sametime. This paper analyzed the group of patients with surgical methods and clinical efficacy. Results CT scan of intreatment group showed that there were 6 cases of secondary operation because of recurrent hemorrhage, and the hematoma-cleared rate>80% in 73 cases,50%-80% in 21 cases,<50% in 10 cases before operation within 6 hours. According to ADL classification,the 92 cases were alive after operation, good recovery in 65 cases, moderate disability in 22 cases, 5 cases of severe disability, death and give up treatment 12 cases. There was 12 cases of sec-ondary operation because of recurrent hemorrhage in control group, and the hematoma-cleared rate>80% in 16 cases,50%-80%in 23 cases,<50%in 24 cases before operation within 6 hours. According to ADL classification,the 45 cases were alive after operation in control group, good recovery in 14 cases,moderate disability in 16 cases,15 cases of se-vere disability,death and give up treatment 18 cases. There were significant differences between the two groups in op-eration time, intra-operative blood loss, the volume of blood transfusion, hematoma clearance rate, postoperative sur-vival rate and the survival quality ADL grading at half a year, and the indexes mentioned above in treatment group were better than those in control group. Conclusion Surgery treatment of hypertensive intracerebral hemorrhage in basal ganglia under direct vision by lateral fissure-brain island approach assisted by the deep cold light suction will shooter the operation time and lower the surgical injury,increase hematoma-cleared rate,it aslo has reliable hemostasis and more effective,so this method is worthy of clinical use.