1.Examination of protective and therapeutic effects of ruscogenin on cerulein-induced experimental acute pancreatitis in rats
Gulcin ERCAN ; Rumeysa İLBAR TARTAR ; Ali SOLMAZ ; Osman Bilgin GULCICEK ; Onur Olgac KARAGULLE ; Serhat MERIC ; Huseyin CAYOREN ; Ramazan KUSASLAN ; Ahu KEMIK ; Damla GOKCEOGLU KAYALI ; Sule CETINEL ; Atilla CELIK
Annals of Surgical Treatment and Research 2019;97(6):271-281
PURPOSE: To determine the potential protective and therapeutic effects and action mechanism of ruscogenin on cerulein-induced acute pancreatitis (AP) model in rats. METHODS: Overall, 32 rats were attenuated to the sham (2-mL/kg/day isotonic solution for 4 weeks), control (20-µg/kg cerulein-induced AP for 12 hours), prophylaxis groups (cerulein-induced AP following 3-mL/kg/day ruscogenin for 4 weeks) and treatment (3-mL/kg/day ruscogenin following cerulein-induced AP for 12 hours). Blood samples were collected for biochemical analysis of nitric oxide synthase 1 (NOS1/neuronal NOS), malondialdehyde (MDA) and intercellular adhesion molecule 1 (ICAM-1). After sacrification, pancreas tissues were collected and prepared for light microscopic (hematoxylin and eosin), immunohistochemical (nuclear factor kappa B) and biochemical analysis (tumor necrosis factor-alpha [TNF-α], interleukin-6 and 1β [IL-6 and IL-1β], CRP, high-sensitivity CRP [hs-CRP] amylase, lipase, and ICAM-1). Ultrastructural analysis was performed by transmission electron microscopy. RESULTS: The protective and therapeutic actions of ruscogenin were accomplished by improvements in histopathology, by decreasing blood cytokine levels of CRP, hs-CRP levels, TNF-α, IL-6, IL-1β, ICAM-1, by reducing the pancreatic enzymes amylase and lipase in blood, and by suppressing the expression of nuclear factor kappa B, ICAM-1, and NOS-1, but not MDA in pancreatic tissues. Ruscogenin also improved cerulein-induced ultrastructural degenerations in endocrine and exocrine cells, especially in treatment group. CONCLUSION: The present findings have demonstrated the beneficial protective and therapeutical effects of ruscogenin, nominating it as a highly promising supplementary agent to be considered in the treatment of AP, and even as a protective agent against the damages induced by disease.
Amylases
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Animals
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Ceruletide
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Intercellular Adhesion Molecule-1
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Interleukin-6
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Lipase
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Malondialdehyde
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Microscopy, Electron, Transmission
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Necrosis
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NF-kappa B
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Nitric Oxide Synthase
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Pancreas
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Pancreatitis
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Rats
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Therapeutic Uses