Objective To compare the dosimetric differences between pencil beam convolution (PBC) and anisotropic analytical algorithm (AAA) in Eclipse treatment planning system for intensitymodulated radiotherapy (IMRT) planning of lung cancer patients and dosimetric verification.Methods 10 IMRT plans of lung cancer patients were calculated using the PBC and AAA and the differences of dosimetric parameter were analyzed according to dose-volume histogram of planning target volume (PTV),lung and spinal cord.The verification measurements were performed on an inhomogeneous thorax phantom using a pinpoint ionization chamber.The agreement between calculated and measured doses was determined.The paired t test was used to compare the results.Results Compared with PBC,the AAA predicted higher maximum PTV dose (t =-4.03,P =0.010),lower minimum PTV dose (t =5.09,P =0.040),and a reduction of the volume of PTV covered by the prescribed dose.The AAA also predicted slightly increases than the PBC algorithm in the mean dose to the lung and the V20 as well as the maximum dose to the spinal cord,and the differences were statistically significant (t =-3.99,-2.79,-5.46,P =0.010,0.038,0.003).In the verification measurements,the agreement between the AAA and measurement was within 2％and superior to the PBC algorithm on isocenter (t =-3.82,P =0.012).Conclusions For IMRT treatment planning of lung cancer,the PBC algorithm overestimates the dose to the PTV and underestimates the dose to the lung and the spinal cord,so the AAA for treating planning in which the tissue inhomogeneous such as lung is present is recommended.

Objective:To introduce estimate a technique ofregulative aperture methodin locating lung block in TBI.Methods:We excavated a square aperture in the foam template which is used to fasten the lung block,and the projection formed by the field light through the aperture is used as the reference for adjusting the location of the lung block.Measure the windage of lung shades and block shades by X-ray,in 8 patients.Results:windage values of head-feet and left-right orientations are smaller inregulative aperture modethan in conventional method respectively.Conclusions:Regulative aperture methodis an easy and effective technique to improve lung shielding precision in TBI.

Objective To develop an ELISA(Enzyme-Linked Immunosorbent Assay)diagnostic kit for early rapid detection of sarum anti-EV71 antibody and evaluate its clinical application value.Methods Recombinant protein VP1 of EV71 were prepared and purified as an immobilized antigen for establishment of an indirect ELISA for detection of serum anti-EV71 IgM and anti-EV71 IgG.Compared with RT-PCR.isolation of EV71 and micro-neutralizing assay.the clinical application value of anti-EV71 IgM and anti-EV71 ISG in the diagnosis of EV71 disease was evaluated.Results In comparison with RT-PCR.the sensitivity,specificity,positive predictive value and negative predictive value of anti-EV71 IgM antibody were 83%,85%,81%and 87%,respectively.The sensitivity,specificity,positive predictive value and negative predictive value of anti-EV71 IgG antibody were 72%,74%,68%and 77%.respectively.Compared with viral isolation assay.the sensitivity and specificity of anti-EV71 IgM antibody were 85%and 97%,respectively.The sensitivity and specificity of anti-EV71 IsG antibody were 75%and 77%,respectively.In addition.the titers of anti-EV71 IgG antibody were significantly correlated with the titers of neutralizing antibody to EV71 by linear regression analysis(r=0.72,P＜0.05).Finally,the serum titers of anti-IgG from patients with EV71 associated hand food and mouth disease at convalescent stage exhibited significantly higher than that of the same patients at acute stage(P＜0.01),but the titers of anti-IgM had no significant difference(P＞0.05).Conclusions With VP1 recombinant protein used as an immobilized antigen,an indirect ELISA diagnostic kit was successfully develooed for detection of serum anti-human EV71 IgM and anti-human EV71 IgG antibodies.

OBJECTIVETo investigate the chest X-ray manifestations of SARS cases.

METHODSA retrospective study was conducted among 52 clinically confirmed SARS patients from February 9 to May 10, 2003. Chest X-ray scanning was performed at a interval of 1 - 3 days according to the requirements. The manifestations and special features of SARS in X-ray were analyzed.

RESULTSSmall or large patchy shadows with intensive density in both lungs were observed in 31 cases, ground-glass like opacification in 16, small patchy shadows in one lung lobe or one lung segment in 18, nodular shadows in one lung segment in 1, and increased lung marking in lung interstitial tissues in 2. Rapidly changing consolidations revealed in chest X-ray images were found to be associated with SARS infections, and they were not affected by treatment with antibiotics.

CONCLUSIONChest X-ray provides a sensitive and specific method for the diagnosis and treatment of SARS, and those present with symptoms and signs should undergo chest X-ray scanning every 1 - 3 days.

Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Female ; Humans ; Male ; Middle Aged ; Radiography, Thoracic ; Retrospective Studies ; Severe Acute Respiratory Syndrome ; diagnostic imaging

BACKGROUNDWith the development of natural orifice trans-luminal endoscopic surgery, studies on transoral video-assisted thyroidectomy in preclinical experiments (e.g., human anatomy and animal trials) were progressing gradually. From 2009 to 2011, embalmed human cadavers were dissected to define the anatomical location, surgical planes, and related neural and vascular structures to create a safe transoral access to the front cervical spaces. Recently, experimental transoral endoscopic thyroidectomy was performed to verify the feasibility of this approach on 15 fresh specimens.

METHODSFifteen specimens were placed in the supine position with slight neck extension. Endoscopic incision was made on the midline between the Wharton's duct papillae and two other incisions were made on mandibular first premolar buccal mucosa. Sublingual combined bilateral vestibular tunnels were created from oral cavity to the cervical region. The neck subplatysmal working space was insufflated with CO2 at 6-8 mmHg. The bilateral thyroid lobes and central lymph nodes were dissected under craniocaudal view.

RESULTSThree incisions were made in the oral cavity without any incisions on the body surfaces. The distance from the oral cavity to front neck region was the shortest. Bilateral thyroid lobes and central neck region were fully resected via transoral approach. This approach provided a craniocaudal view, in which retrosternal thyroid gland and lymph nodes were easily accessible. The recurrent laryngeal nerve could be identified safely on the inferior cornu of the thyroid cartilage. The only structure at risk was the mental nerve. Camera motion was somewhat limited by the maxillary dentition. The volume of harvested thyroid nodule through sublingual tunnel in the fifteen human cadavers was (40 ± 15) cm(3).

CONCLUSIONThe transoral procedure is progressive and innovative which not only gives the best cosmetic result and minimal access trauma but also provides a craniocaudal view.

Cadaver ; Endoscopy ; methods ; Female ; Humans ; Male ; Neck Dissection ; methods ; Thyroidectomy ; Video-Assisted Surgery ; methods