Flavonoid is one of interested compounds in treatment for cancers. The authors extracts flavonoid from the leaves Houttuynia cordata and tested on S-180 cancer cells that they cultured vitro in mice. This product has effects by inhibited the growth of ascite cancer. However, the studies on large mice models are needed to survey their survival, mortality as well as clinical course, biochemical, pathology, and side effects. Flavonoid has cytotoxic activity on S-180, and maintain excessive cytotoxic on that cells
Neoplasms ; Flavonoids ; Houttuynia ; Flavonoids

The antibacterial effects of flavonoid substances isolated from plants belonging to C.clerodendron species were studied. They are included Clerodendron fragrant, C.informatum, C.cytophyllum. The results showed that flavonoids from these plants presented antibacterial activities on both strain of Gram (+) and Gram(-) but stronger effect obtained with Gram(+). The antifungal activity was week or not resulted for all these three plants. The mixed flavonoid solutions may give better antibacterial effects depending on the combination of extracted flavonoid solutions.
Flavonoids ; Verbenaceae ; Pharmaceutical Preparations

The parameters of pH, albumin of the calf serum, which produced in Poliomyelitis Vaccine Research and Production Center, are higher than standard sample of Gibco Company. In general, the other parameters such as hemoglobin, protein, and bilirubin were lower than the standard sample. The viscidity of study samples is equivalent to standard sample. The optimum serum - supplemented concentration in the medium for cell culture is 10%. The viscidity clearly effect to the growth of the cells get from the medium for monkey kidney cell growth when add the calf serum- product of Polimyelitis vaccine research and production center is 82.16% in comparing with the standard sample.
serum ; vaccines ; diagnosis ; Poliomyelitis

The parameters of pH, albumin of the calf serum, which produced in Poliomyelitis Vaccine Research and Production Center, are higher than standard sample of Gibco Company. In general, the other parameters such as hemoglobin, protein, and bilirubin were lower than the standard sample. The viscidity of study samples is equivalent to standard sample. The optimum serum - supplemented concentration in the medium for cell culture is 10%. The viscidity clearly effect to the growth of the cells get from the medium for monkey kidney cell growth when add the calf serum- product of Polimyelitis vaccine research and production center is 82.16% in comparing with the standard sample.
serum ; vaccines ; diagnosis ; Poliomyelitis

The new flavonoid was isolated from the leaves of clerodendron fragrans Vent. Its structure was confirmed by various spectrum of IR, UV, MS, 1H, C13-NMR, DEPT, COSY as 5, 7, 8-trihydroxy-4, -methoxy flavone (I). That flavonoid composition presented good antioxiadant activity. Its effect on cathepsin activity in human blood was studied and showed that flavonoid increased cathepsin activity at optimum concentration.
Biological Factors ; Clerodendrum

The study on antioxydative activities of the polarizied of BoBo on order to expoit its pharmaco-biological effect efficiently. Results showed that antioxydative activity at cell membrane lipid invitro and invivo of 14 extracted products of Bo Bo leaf Bts, Bn, B1-B12 was evaluated Dn invitro, experiment dose of 0,1 mg/ml of Bts, B1,B2, B3, B4,B5 manifested significant antioxydant activities, among then Bts and B5 have the highest activity, their preparations have the highest inhibition quality
Antioxidants ; Plants, medicinal ; Biological Markers

Background: The fetus having risk for Down sydrome (DS) can be detected early by AFP, \u03b2hCG in maternal serum to detect the fetus having risk of Down sydrome. Objectives: Determining values of AFP, \u03b2hCG in maternal serum to detect the fetus having risk of DS. Subjects and method: Determining the concentration of AFP, \u03b2hCG in maternal serum of 591 pregnant with fetus \ufffd?12 weeks to detect the fetus having DS screening risk. The fetus are diagnosed DS by analysing chromosome from amniocyte and monitor up to the neonate. Then, finding out association between DS fetus and AFP, \u03b2hCG in maternal serum. Results: 75/591 of subjects screened were screen positive, 6/7 DS fetus associated withscreen positive, (cut off AFP \ufffd?0,75 MoM, \u03b2hCG \ufffd?2,2 MoM). Detection rate (DR) was 85,71%; false positive rate (FDR): 11,81%. Only base on AFP: DR was 71,43%; FDR: 11,81%. Only base on \u03b2hCG DR was 28,57%; FDR: 0,51%. Conclusion: DR base on AFP is higher than base on \u03b2hCG. If combining AFP and \u03b2hCG, DR is the highest. AFP is important role in screening DS fetus.
Down Syndrome/ diagnosis ; Predictive Value of Tests ; Prenatal Diagnosis ; Pregnancy ;

Background: Down syndrome is a developmental disorder caused by an extra copy of chromosome 21, is a condition in which extra genetic material causes delays in the way a child develops, both mentally and physically. It affects about 1 in every 700 babies. The fetus having high risk for Down syndrome (OS) can be detected early by ultrasound. Objectives: The aim of the study is to find out some ultrasound markers that relate to OS fetus. Subjects and method: A descriptive study was carried out on 612 pregnant women with fetus \ufffd?12 weeks by ultrasound to detect abnormal markers in fetus. The fetus were diagnosed Down syndrome by analysis chromosome from amniocyte and monitor up to the neonate. Then, finding out association between OS fetus and ultrasound markers. Results: Among 612 pregnant women,36/12 pregnant women had abnormal imaging in fetus, 11/12 pregnant women had OS fetus. There were 12 pregnant women detected OS fetus. 6/12 OS fetus associated with the maker of nuchal skin fold (cut off 2: 3mm at the first trimester and 2: 6 mm at the second trimester): Detection rate (DR) was 50%; false positive rate (FOR): 0,83%. 3/12 OS fetus associated with the marker of duodenal atresia. DR was 25%; FOR: 0%. Conclusions: The two common markers associated with OS fetus: \r\n', u'the first marker was nuchal skin fold (with cut off 2: 3mm at the first trimester and > 6 mm at the second trimester) and the second marker was duodenal atresia. \r\n', u' \r\n', u'
Down Syndrome ; Fetus/ anatomy & ; histology ; abnormalities ; physiopathology ; ultrasonography

Background: Fragile X Syndrome (FXS) is the second cause of Mental Retardation (MR) and the first cause of familiar MR. This syndrome affects up to 1/4000 men and 1/8000 women. X syndrome is often diagnosed by molecular biology technique such as RCR and Southern blot. Until now there is no study on FXS in Vietnam. Objectives: This study is aimed at: (1) Determine FXS among children with MR by technique of molecular biology. (2) Determine the mutation of FMR1 gen in families having children with FXS. Subject and Method: 214 children between 6 and 16 years of age (136 male and 78 female) with MR were analyzed FMR1 gen by PCR and Southern blot techniques. Families of children with FXS were also analyzed. Result and conclusion: This is the first study on FXS using the techniques of molecular biology in Vietnam. Identified 3 children with FXS, accounting for 1.4% of MR. Children with FXS and members with full mutation and premutation were found.
Fragile X syndrome ; FMR1 gene ; Mental retardation

Background: FISH can detect number and structural chromosome aberrations in DNA. FISH is new technique in Vietnam, we combine FISH with chromosome analysis to prenatal diagnosis Down syndrome and turner syndrome that are high rate in birth defect.Objectives: To detect Down syndrome and turner syndrome by using FISH technique with chromosome analysis from amniotic cell.Subjects and method: 14amniotic cells samples 15th - 20th week with high risk of birth defects. Advance using FISH and chromosome analysis from amniotic cell. Results: We obtained results as follow: - 14/14 samples: correspondence between FISH and chromosome analysis. \ufffd?Detected 2 Down syndrome (female. Trisomi 21) and 4 Turner syndrome (45, X). Conclusion: Detected Down syndrome and Turner syndrome by using FISH technique with chromosome analysis from amniotic cell.
Down Syndrome/ diagnosis ; Turner Syndrome/ diagnosis ; Predictive Value of Tests ; Prenatal Diagnosis ; In Situ Hybridization ; Fluorescence ;