1.Determination of scandoside methyl ester in Hedyotis chrysotricha by HPLC.
Jing LI ; Guoping ZHOU ; Caitang LI ; Ping WEN ; Ping YING ; Jinbao YU ; Huodi WU
China Journal of Chinese Materia Medica 2009;34(20):2619-2621
OBJECTIVETo develop an HPLC method for determination of scandoside methyl ester in Hedyotis chrysotricha.
METHODThe determination was carried out on an HC-C18 column elnted with acetonitrile-water (7:93) as mobile phase, and the detection wavelength was set at 238 nm.
RESULTThere is a good linearity in the range 22.08-552 mg L(-1) of scandoside methyl ester and the average recovery is 97.7% (n = 6), RSD 0.72%.
CONCLUSIONThis method is convenient, quick, and is applicable to the quality control of H. chrysotricha.
Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; Esters ; analysis ; Hedyotis ; chemistry
Result Analysis
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