OBJECTIVE: To observe monocyte chemoattractant protein 1 (MCP-1) changes in ischemic tissue during the process of angiogenesis induction in ischemic limbs by autologous mesenchymal stem cell (MSC) transplantation.METHODS: Twenty male Sprague-Dawley rats were randomized to 2 groups (n = 10): model and MSC transplantation. Femoral and tibial bone marrow was taken to isolate and culture MSCs by percoll density gradient method. Cells of the 3~(rd) or 4~(th) passage were used for transplantation. Severe bilateral hind limb ischemia was surgically created in each group rats. Two hours after model establishment, MSCs (1×10~(11)/L) were infused into the ischemic region of rats from the MSC transplantation group, and the model group received the same amount of phosphate buffered saline. Collateral artery formation was determined by angiographic analysis and histological assessment. CD68~+ macrophage infiltration was examined by immunohistochemistry. MCP-1 protein expression in the plasma and ischemic tissue was detected by enzyme-linked immunosorbent assay. MCP-1 mRNA expression in ischemic tissue was detected by reverse transcription-polymerase chain reaction.RESULTS: At postoperative 28 days, treatment with MSC transplantation lead to collateral vessel formation, and immunohistochemistry demonstrated that CD68~+ macrophage infiltration was lower compared with the model group. MCP-1 protein and mRNA expression in the plasma and ischemia tissue was significantly lower in the MSC transplantation group than in the model group (P < 0.05).CONCLUSION: Following MSC transplantation, MCP-1 may play an important role in ischemia-induced angiogenesis. This indicates that MCP-1 would become one possible target molecule for modulating inflammatory angiogenesis by MSC Transplantation.

BACKGROUND:Spinal internal fixation is widely used in the treatment of lumbar spondylolisthesis, with the purpose of lumbar fusion, reconstruction of lumbar sagittal sequences, relieving spinal cord or nerve root compression. OBJECTIVE:To review the biocompatibility of lumbar spinal implants applied in the treatment of degenerative lumbar spondylolisthesis. METHODS:A computer-based retrieval of PubMed and CNKI was performed for relevant articles published from January 2000 to January 2015. The keywords were biocompatibility, degenerative lumbar spondylolisthesis, implant in English and Chinese, respectively, which appeared in the title and abstract. RESULTS AND CONCLUSION:Lumbar spinal implants commonly used include pedicle screws and interbody fusion cages. The fusion cages are mainly classified as biological fusion cage, metal fusion cage, composite fusion cage and absorbable fusion cage. Internal fixation implants made of different materials have different biocompatibilities, but also develop different stabilities. With the development of bionics, 3D printing technology, biomechanics and materials science, lumbar spinal implants wil have better biocompatibility, bone substitute capability and biomechanical properties.

Objective To observe the effects of valsartan and propranolol on the colonic mucosal microcirculation and submucosal ultra-structure changes in rats with portal hypertensive colopathy (PHC).Methods Portal hypertension(PHT) with cirrhosis was induced by composite factors after 42 days in rats.Rats were divided into a normal control group,a cirrhotic PHT model group,a treatment group with valsartan 20 mg/kg once daily,a treatment group with propranolol 22.5 mg/kg twice daily and a combination treatment group with propranolol and valsartan.The rats were treated for 15 days. The rats in the normal control group and the cirrhotic PHT model group were given water only.At the end of study,portal venous pressures(PVP) were measured.The submucosal vascular areas and metrical diameters of phlehectasia were measured by light microscope.The ultra-structure was observed by trans mission electron microscope.Results Compared to the cirrhotic PHT model group,PVPs were significantly decreased in the valsartan,propranolol,and combined groups (P

Objective To evaluate the use of endovascular aortic repair(EVAR) and open aortic surgery(IAS) for treatment of abdominal aortic aneurysm.Methods A retrospective review of patients treated with EVAR or IAS between 2002 and 2007 was performed.The occurrence rate of perioperative complications,morbidity and mortality,survival rate,quality of life,and cost of treatment were compared.Results Forty-two patients(30INS,12EVAR) were treated.Operating time,the intraoperative blood loss,and intraoperative blood transfusion were less in EVAR group than in the IAS group(P

Objective To study the clinical effects of endovascular stent-graft repair for thoracic aortic aneurysm(TDA).Methods The clinical records of 32 patients with thoracic aorta dissecting aneurysm,including 26 men and 6 women,who underwent endovascular repair were retrospectively analyzed.The characteristics,location,and anatomic condition of dissecting aneurysm were evaluated using CTA before operation.Under general or local anesthesia,32patients underwent endovascular repair under dynamic DSA.For those patient,8 Talent,22 Yuhengjia(Beijing) and 2 Microinvasive(Shanghai) stent grafts were used.Results Among them 32 patients the distance between entry tear and left subclavian artery was

Humans ; Spleen/surgery* ; Tomography, X-Ray Computed ; Disability Evaluation

Objective To observe the influence of technetium [99Tc] methylenedipho-honate (99Tc-MDP) on osteoclastogenesis induced by receptor activator of NF-κB ligand (RANKL) and macrophage-colony stimulating factor (M-CSF)in peripheral blood mononuclear cells in patients with rheumatoid arthritis, and to study the mechanism of 99Tc-MDP in osteoclast differentiation. Methods The monocytes/macrophages were isolated from peripheral blood in patients with rheumatoid arthri-tis, incubated in RPMI-1640 with receptor activator of NF-κB ligand (RANKL, 25 μg/L), macro-phage-colony stimulating factor (M-CSF, 25μg/L ) and different concentrations of 99Tc-MDP (5, 10, 20,and 50 mg/L) for 4,12, and 20 days. Tartrate resistant acid phosphatase staining was used to observe the formation of osteoclasts. Results After 12 or 16 days culture of peripheral blood mononuclear cells, plenty of large nultinuclear cells could be found on the coverslips. 99Tc-MDP markedly inhibited those changes and the inhibitory effects were increased as the concentration of 99Tc-MDP increased (P<0.05). Conclusion 99Tc-MDP probably has some protective effect on rheumatoid arthritis by inhibiting osteoclast formation.

AIM: To study the bioequivalence of piracetam tablets in Chinese healthy volunteers. METH-ODS: Twenty volunteers were randomly divided into two groups (test and reference), with double cross-over design and single-dose oral administration. The concentration of piracetam in serum was determined by HPLC. The main pharmacokinetic parameters were calculated and the bioequivalence was evaluated with DAS2.0 practical pharmacokinetics program. RESULTS: The pharmacokinetic parameters of piracetam tablets were as follows: t1/2 were 5.50±1.48 and 4.29±1.00 h, Cmax were 21.47± 6.27 and 20.96±5.10 mg·L-1, Tmax were 0.70±0.46 and 0.66± 0.36 h, AUC0-24h were 93.44± 16.61 and 96.67± 18.50 mg·h·L- 1. The relative bioavailability of the test preparation was 99.8%± 22.7%. CONCLU-SION: The test and reference preparations were bioequivalent and may be prescribed interchangeably.

Objective To investigate the dynamic change of procalcitonin (PCT ) and hypersensitive C‐reactive protein (hs‐CRP) in neonatal infection and its clinical significance .Methods 176 neonates with infection in our hospital from August 2013 to July 2014 were selected and treated by the routine therapy of anti‐infection ,oxygen inhalation ,etc .,and divided into the improvement group(89 cases) ,non‐change group(48 cases) and deterioration group(39 cases) according to the curative effect after 20 d therapy . Serum PCT and hs‐CRP levels were detected before treatment(T0) ,on 5 d(T1) ,10 d(T2) ,15 d(T3) and 20 d(T4) of treatment by adopting the enzyme linked immunosorbent assay(ELISA) .Results The PCT ,hs‐CRP levels and WBC count before treatment had no significant difference among 3 groups(P>0 .05);the PCT and hs‐CRP levels after treatment in the improvement group showed the significantly declining trend ,which reached the lowest at T4 (P<0 .05) ,while the PCT and hs‐CRP levels in the deterioration group showed the obviously rising trend ,which reached the highest at T4 (P<0 .05) ,but which in the non‐change group had no significant change (P>0 .05) .In terms of WBC count ,the WBC count in all the cases showed a rising first and then decreasing trend ,the WBC count at T4 in the improvement group was lower than before treatment(P<0 .05) ,while which in the deterioration group was higher than before treatment (P<0 .05) ,but which in the non‐change group had no significant change between before and after treatment(P>0 .05);the Spearman analysis results after treatment showed that the serum PCT ,hs‐CRP levels and WBC count were negatively correlated with efficacy (r1 = -0 .764 ,P1 <0 .05;r2 = -0 .724 ,P2 <0 .05 ;r3 = -0 .573 ,P3 <0 .05) .Conclu‐sion PCT and hs‐CRP can effectively reflect the therapeutic effect of neonatal infection ,and is closely correlated with the patient′s own immune status ,which can be taken as one of reference indicators for formulating the treatment scheme and evaluating progno‐sis ,and is worth for further clinical promotion .

The integrity of the neuronal membrane is important for its specific physiological function.Spinal cord injury (SCI) can result in immediate disruption of neuronal membranes.Now,a novel treatment method for SCI is sealing the compromised membranes at the early stage of SCI.In this article,recent development of membrane repair agents in treating SCI is discussed,including polyethylene glycol(PEG),poloxamer 188(P188),chitosan and some nanomaterials.At the same time,problems remained in recent researches are reviewed and the prospect is discussed.