OBJECTIVE: To observe monocyte chemoattractant protein 1 (MCP-1) changes in ischemic tissue during the process of angiogenesis induction in ischemic limbs by autologous mesenchymal stem cell (MSC) transplantation.METHODS: Twenty male Sprague-Dawley rats were randomized to 2 groups (n = 10): model and MSC transplantation. Femoral and tibial bone marrow was taken to isolate and culture MSCs by percoll density gradient method. Cells of the 3~(rd) or 4~(th) passage were used for transplantation. Severe bilateral hind limb ischemia was surgically created in each group rats. Two hours after model establishment, MSCs (1×10~(11)/L) were infused into the ischemic region of rats from the MSC transplantation group, and the model group received the same amount of phosphate buffered saline. Collateral artery formation was determined by angiographic analysis and histological assessment. CD68~+ macrophage infiltration was examined by immunohistochemistry. MCP-1 protein expression in the plasma and ischemic tissue was detected by enzyme-linked immunosorbent assay. MCP-1 mRNA expression in ischemic tissue was detected by reverse transcription-polymerase chain reaction.RESULTS: At postoperative 28 days, treatment with MSC transplantation lead to collateral vessel formation, and immunohistochemistry demonstrated that CD68~+ macrophage infiltration was lower compared with the model group. MCP-1 protein and mRNA expression in the plasma and ischemia tissue was significantly lower in the MSC transplantation group than in the model group (P < 0.05).CONCLUSION: Following MSC transplantation, MCP-1 may play an important role in ischemia-induced angiogenesis. This indicates that MCP-1 would become one possible target molecule for modulating inflammatory angiogenesis by MSC Transplantation.

BACKGROUND:Spinal internal fixation is widely used in the treatment of lumbar spondylolisthesis, with the purpose of lumbar fusion, reconstruction of lumbar sagittal sequences, relieving spinal cord or nerve root compression. OBJECTIVE:To review the biocompatibility of lumbar spinal implants applied in the treatment of degenerative lumbar spondylolisthesis. METHODS:A computer-based retrieval of PubMed and CNKI was performed for relevant articles published from January 2000 to January 2015. The keywords were biocompatibility, degenerative lumbar spondylolisthesis, implant in English and Chinese, respectively, which appeared in the title and abstract. RESULTS AND CONCLUSION:Lumbar spinal implants commonly used include pedicle screws and interbody fusion cages. The fusion cages are mainly classified as biological fusion cage, metal fusion cage, composite fusion cage and absorbable fusion cage. Internal fixation implants made of different materials have different biocompatibilities, but also develop different stabilities. With the development of bionics, 3D printing technology, biomechanics and materials science, lumbar spinal implants wil have better biocompatibility, bone substitute capability and biomechanical properties.

Objective To evaluate the use of endovascular aortic repair(EVAR) and open aortic surgery(IAS) for treatment of abdominal aortic aneurysm.Methods A retrospective review of patients treated with EVAR or IAS between 2002 and 2007 was performed.The occurrence rate of perioperative complications,morbidity and mortality,survival rate,quality of life,and cost of treatment were compared.Results Forty-two patients(30INS,12EVAR) were treated.Operating time,the intraoperative blood loss,and intraoperative blood transfusion were less in EVAR group than in the IAS group(P

Objective To study the clinical effects of endovascular stent-graft repair for thoracic aortic aneurysm(TDA).Methods The clinical records of 32 patients with thoracic aorta dissecting aneurysm,including 26 men and 6 women,who underwent endovascular repair were retrospectively analyzed.The characteristics,location,and anatomic condition of dissecting aneurysm were evaluated using CTA before operation.Under general or local anesthesia,32patients underwent endovascular repair under dynamic DSA.For those patient,8 Talent,22 Yuhengjia(Beijing) and 2 Microinvasive(Shanghai) stent grafts were used.Results Among them 32 patients the distance between entry tear and left subclavian artery was

Objective To observe the effects of valsartan and propranolol on the colonic mucosal microcirculation and submucosal ultra-structure changes in rats with portal hypertensive colopathy (PHC).Methods Portal hypertension(PHT) with cirrhosis was induced by composite factors after 42 days in rats.Rats were divided into a normal control group,a cirrhotic PHT model group,a treatment group with valsartan 20 mg/kg once daily,a treatment group with propranolol 22.5 mg/kg twice daily and a combination treatment group with propranolol and valsartan.The rats were treated for 15 days. The rats in the normal control group and the cirrhotic PHT model group were given water only.At the end of study,portal venous pressures(PVP) were measured.The submucosal vascular areas and metrical diameters of phlehectasia were measured by light microscope.The ultra-structure was observed by trans mission electron microscope.Results Compared to the cirrhotic PHT model group,PVPs were significantly decreased in the valsartan,propranolol,and combined groups (P

Humans ; Spleen/surgery* ; Tomography, X-Ray Computed ; Disability Evaluation

Objective To explore the role of procalcitonin(PCT)in antibiotics therapy of elder patients with community acquired pneumonia(CAP). Methods Sixty-two elderly patients with CAP who hospitalized in Linxi Hospital of Kailuan General Hospital from Jan. 2010 to Dec. 2013 were selected as study subjects,and their age span were 54. 0 - 87. 0 years old. All the patients were randomly divided into conventional therapy group(n= 33)and PCT group(n = 29). The use of nntibiotics in conventional treatment group was based on clinical symptoms of patients and the standard of antibiotics application in clinic,while in PCT group was based on PCT serum levels. The main observation indexes including days of antibiotics use,cost of antibiotics use,hospital stay, total cost of hospital stay,clinical efficacy,aggravating cases and death cases. Results The days of antibiotics use,cost of antibiotics use,hospital stay,total cost of hospital stay in PCT group were((12. 2 ± 1. 4)d, (3 341. 9 ± 52. 73)yuan,(12. 1 ± 1. 8)d,(5 346. 2 ± 1 274. 5)yuan)lower than that in conventional therapy group((16. 9 ± 3. 5)d,(3 816. 28 ± 84. 27)yuan,(14. 7 ± 2. 9)d,(6 653. 2 ± 1 132. 8)yuan),and the differences were significant(t = 6. 77,26. 14,4. 17,4. 28;P < 0. 001). There were no significant difference in terms of clinical prognosis( clinical efficacy,aggravating cases and death cases)( P > 0. 05). Conclusion Procalcitonin can guide antibiotics application in the elderly patients,and then prevent the abuse of antibiotics.

The integrity of the neuronal membrane is important for its specific physiological function.Spinal cord injury (SCI) can result in immediate disruption of neuronal membranes.Now,a novel treatment method for SCI is sealing the compromised membranes at the early stage of SCI.In this article,recent development of membrane repair agents in treating SCI is discussed,including polyethylene glycol(PEG),poloxamer 188(P188),chitosan and some nanomaterials.At the same time,problems remained in recent researches are reviewed and the prospect is discussed.

Fifty-one cases of menopause syndrome were randomly divided into three groups, 21 cases in the acupuncture and cupping group, 10 cases in the acupuncture group, and 20 cases in the ear acupuncture group, and were respectively treated by acupuncture plus cupping therapy, acupuncture,and ear acupuncture. The total effective rate was respectively 80.9%, 20.0% and 80.0%. The therapeutic effect was better in the treatment of menopause syndrome by acupuncture plus cupping therapy than by single acupuncture treatment or ear acupuncture (P＜0.05).

Objective To investigate the clinical value and characteristics of color Doppler ultrasound in the diagnosis of fetal ductus arteriosus narrow.Methods 22 pregnant women were screened by ultrasound in the hospital (the disease group),In the same period,50 cases of fetus in pregnant women were selected as control group,The arterial catheter inside diameter,systolic and diastolic velocity flow were compared,and different facets of the ductus arteriosus in ultrasonography were observed.Results The ductus arteriosus diameter of disease group gestational age (28-36 weeks) and gestational age (＞ 36 weeks) were (2.25 ± 0.68) mm and (4.29 ± 0.14) mm,which were significantly lower than those in the control group(t =3.01,2.98,all P ＜ 0.05).The systolic flow rate of disease group gestational age (28-36 weeks) was (0.39 ± 0.20) m/s,which was significantly higher than (0.32 ± 0.31) m/s in the control group (t =2.96,P ＜ 0.05).The systolic and diastolic flow velocity of disease group gestational age (＞ 36 weeks) were (0.42 ± 0.17) m/s,and (0.24 ± 0.08) m/s,which were significantly higher than those of the control group (t =2.91,3.04,all P ＜ 0.05).The color Doppler ultrasound of fetal ductus arteriosus narrow showed arterial catheter lumen thinner,blood flow velocity was significantly faster,including five cases of arterial stenosis showed right atrium,right ventricle; 3 cases showed the right atrium and right ventricle increased,with severe tricuspid regurgitation.Conclusion Color Doppler ultrasound can identify fetal ductus arteriosus braid and can objectively and comprehensively assess it.It has important diagnostic value for fetal ductus arteriosus stenosis.