Adult wounds heal with scar formation, whereas fetal wounds heal without scarring and with a lesser inflammatory and cytokine response. Recently connective tissue growth factor (CTGF) is known to play an important role in wound healing. We reasoned that a strategy employing antisense oligodeoxynucleotides (ODN) complementary to CTGF mRNA by topical application of the ODN on the skin wound. Phosphorothioation of ODN to retard their degradation. When antisense CTGF ODN were applied on the wound site, there was a marked reduction of scarring compared with a control wound site. This effect of antisense CTGF ODN on scar forrnation was associated with decreased expression of the CTGF gene. However, sense CTGF ODN had no effect on the expression of the CTGF gene. In addition, control wounds healed with excessive fibrosis compared with the antisense-treated wounds. In conclusion, our results indicate that antisense CTGF ODN could be used for ameliorating scar formation during wound healing.
Adult* ; Cicatrix* ; Connective Tissue Growth Factor* ; Connective Tissue* ; Fibrosis ; Humans ; Oligodeoxyribonucleotides ; RNA, Messenger ; Skin ; Wound Healing ; Wounds and Injuries*

No abstract available.
Tendons*

Congenital dislocation of the hip has always presented an unique problem in Orthopedic Surgery. Orthopedic surgeons have been baffled not only by the mystery of its origin, but also by its unpredictable course and the uncertainty of results of treatment. Hippocrates described this condition first with all its distinguishing characteristics, including distortion of the hip, the pathologic gait, the exaggerated lordosis. etc, His prognosis in this luxation of early childhood appeared to be unfavorable. In 1895, Lorenz established a new, decidedly conservative method of treatment. His process involved a complete separation of the two objectives to be fulfilled, namely reduction and retention. To retain this reduction he conceived the motion of fixing the hip for a specific and prolonged period in carefully chosen “primary position” of 90 flexion and 90 abduction by forcibly maintaining the head in its normal location. Ortolani, in 1935, was the first to show that a diagnosis could be establisted immediately after birth. The jerk, click or snap elicited by manipulation of the new born infants hip. Early diagnosis is still the most important aspect of congenital disiocation of the hip. Despite the emphasis on early diagnosis, a disturbingly large number of congenital dislocation of the hip remain undiagnosed until after the child has started to walk. Numerous surgical procedures and modifications have been used. The general types are: 1) Open reduction, 2) Shelf procedure, 3) Innominate osteotomy, 4) Arthroplasty, and 5) femoral osteotomy. These procedures have been useful also with the more difficult problem of residual or recurrent dislocation or subluxation aftertreatment by closed or open method. Congenital dislocation of the hip was not common in Korea like as in western countries. The reasons for this are poorly understood. But racial differences and native customs may offer a partial explanation. The author studied congenital dislocation of the hips in sex incidence, site of predilection, clinical symptoms and signs, X-ray findings, relation to the congenital anomalies and birth history, and the results of treatment. In this study, 57 cases of congenital dislocation of the hip, admitted to the orthopedic department of Severance Hospital during 7 years and 6 months since January 1963 until June 1970, were reviewed. The results of this study are as follows: 1. The annual changes of the numbers of the patients, who were first seen at Severance Hospital, were increasing. 2. The female incidence in Korea is relatively low as compared with the western countries. And the preponderance of females over males is in the ratio of ten to one in bilateral cases and two to one in unilateral cases. 3. The ratio of bilateral cases: right sides: left sides is 1:2: 2.2. 4. In these series, 15 cases of congenital anomalies or diseases in 13 patients are combined and among them, 10 patients are included in teratologic groups. And most common co-existing anomalies are club foot (7 cases). The incidence of the teratologic dislocation in Korea is relatively high as compared with the western countries. 5. The acetabualr index in affected hips showed no relationship to the age factor, but C-E angle, Y-coordinate and height are aggravated according to the increment of age. 6. Closed reduction is satisfactory under the age of 3, but after then the prognosis is poor. 7. Open reduction is recommendable after the age of 3 and under the age of 9. 8. The prognosis of treatment in bilateral cases and teratologic groups is poor.
Age Factors ; Animals ; Arthroplasty ; Child ; Diagnosis ; Dislocations ; Early Diagnosis ; Female ; Foot ; Gait ; Head ; Hip ; Humans ; Incidence ; Infant ; Korea ; Lordosis ; Male ; Methods ; Orthopedics ; Osteotomy ; Parturition ; Prognosis ; Reproductive History ; Surgeons ; Uncertainty

No Abstract Available.

No abstract available.
Anemia, Iron-Deficiency* ; Erythrocyte Indices* ; Iron*

No abstract available.
Child* ; Glomerulonephritis* ; Humans ; Nephrotic Syndrome* ; Plasma* ; Renin*

The concentration of serum triglyceride values were studied at department of pediatrics, Chungnam National University Hospital. With the total 742 cases, among them 462 boys and 280 girls who were at the age of 2 months through 15 years old. Serum triglycerde values were measured to determine the distribution of values and evaluated on the basis of sex, age, height and weight percentile. The following results were obtained: 1) The mean value of the serum triglycerides were 88.11+/-35.76 mg/dl in boys and 94.82+/-34.56 mg/dl in girls. 2) In distribution of trglyceride level by percentile, the value of 90th percentile was 136 mg/dl. 3) The values of the serum triglyceride from 6 to 11 year old age group were significant low values in boys and girls.
Adolescent ; Child* ; Chungcheongnam-do ; Female ; Humans ; Pediatrics ; Triglycerides*

No abstract available.

No abstract available.
Asthma* ; Child* ; Humans ; Sinusitis*

BACKGROUND: The epidermal permeability barrier necessary for terrestrial life resides in the intercellular spaces of the stratum corneum and is composed of lipids. OBJECTIVE: Since strrtum corneum lipid may be important for the permeability barrier, we studied the differences and effects of experimentally altered barrier function using acetone and tape-stripping technique. METHODS: The permeability barrier of hairless mouse was disrupted by tape-stripping and acetone treatment and the recovery rate was assessed by histochemical staining, electron microscopic examination and lipid analysis. RESULTS: Although the transepidermal water loss recovered completely by 48 hours in both of the acute models, acetone treated samples seem to have on over-all better recovery rate than tape-stripped samples. The return of barrier function to normal in both tape-stripped and acetone-treated skin was accompanied by a comparable return of normal nile red and ruthenium tetroxide staining. The amount of lipid in stratum corneum paralleled both the return of barrier function towards normal and the extent of prior damage to the barrier in acetone treated skin, yet, the lipid synthesis in tape-stripped skin showed a slower return of lipid content. CONCLUSION: The difference in the recovery rate of the two acute models may be due to the fact that acetone mainly extracts intercellular lipids, whereas, tape-stripping has a prolonged effect by removal of comeocyte in addition to the intercellular lipids. This shows the importance of comeocytes as well as the intercellular lipid bilayer in the recovery of normal barrier function.
Acetone* ; Animals ; Extracellular Space ; Lipid Bilayers ; Mice ; Mice, Hairless ; Permeability ; Ruthenium ; Skin ; Water