Objective To understand the residual risk of transfusion blood donation in the native region and to conduct the sim-ple comparison of Roche diagnostic company′s first generation and second generation nucleic acid combined detection reagents . Methods The menstrual blood specimens qualified by the routine test in this center (serology test and enzyme immunodetection as-say results were negative) from May to July 2013 were selected and performed the 6-specimen mixed sample test by adopting the Roche Diagnostics company′s first-generation and second-generation nucleic acid combined detection reagents in the United Roche COBAS s 201 operating system ,if the test had the reactivity ,the nucleic acid identification tests and other complementary serologi-cal testing were performed .Results 9 417 specimens were detected ,8 cases of nucleic acid positive were screened out with the posi-tive rate of 0 .85 ‰ ,through the detection of nucleic acid identification reagents ,4 specimens were confirmed as positive for HBV nucleic acid ,1 case was positive for HCV nucleic acids and the rest 3 cases of uncertain HBV-positive .In addition ,with the first generation reagent (reference reagents) as the relative standard ,the second generation reagents (assessment reagent) had the over-all positive coincidence rate of 66 .67% ,the negative coincidence rate of 99 .98% and the total coincidence rate of 99 .96% .In addi-tion ,no HIV nucleic acid positive specimens was found by the nucleic acid test .Conclusion The nucleic acid detection technique can effectively shorten window period,further improve the safety of blood transfusions ,at the same time the overall conformance situ-ation of the Roche′s first-generation nucleic acid combined detection reagents and the second generation combined detection reagents is good ,but there are still lesser differences in the detection of HBV .

Carcinoma ; diagnosis ; Granuloma, Plasma Cell ; diagnosis ; Humans ; Laryngeal Neoplasms ; diagnosis ; Larynx ; pathology

Reflex epilepsy is a type of partial or generalized seizure induced by specific or nonspecific stimulation in individuals without a prior history of seizures. Mahjong epilepsy is a special form of complex reflex epilepsy induced by playing or watching mahjong, with a low incidence rate and complex pathogenesis. Due to the lack of comprehensive understanding, clinical studies of mahjong epilepsy are still mainly based on case reports. Now, we will analyze and summarize the etiology, pathogenesis, clinical diagnosis, electroencephalogram, treatment, and prognosis of mahjong epilepsy to raise awareness of mahjong epilepsy among clinical medical workers.

【Objective】 To detect the piperacillin and amoxicillin antibodies in suspicious blood samples from pre-transfusion compatibility tests in Wuxi and analyze the general characteristics of them, so as to eliminate the interference of drug-induced antibodies with compatibility tests and provide reference for safe and effective blood transfusion, 【Methods】 Drug-sensitized RBCs and low-ion anti-globulin microcolumn gels were used to detect piperacillin and amoxicillin antibodies in 128 plasma samples which were initially undetermined in pre-transfusion compatibility tests. Data were analyzed by Chi-square test or fisher′s exact test. P<0.05 was statistically significant. 【Results】 Among these 128 undetermined samples, including 31 cases of type A, 48 type B, 14 type AB and 35 type O, the overall positive rate of piperacillin and amoxicillin antibodies was 28.9%(37/128), in which the positive rates of piperacillin and amoxicillin antibodies were 20.3%(26/128) and 8.6%(11/128), respectively. The difference between these two drug-induced antibodies was significant(P<0.05). Further analysis showed that the piperacillin antibodies in patients over 50 years old was 25.3%(24/95), while under 50 years old was 6.1%(2/33)(P<0.05). In contrast, the amoxicillin antibodies in patients over 50 years old was 5.3%(5/95), while under 50 years old was 18.2%(6/33), with statistically significant differences between each other(P<0.05). 【Conclusions】 In patients with suspicious antibodies in pre-transfusion detection, except for the allotype antibodies, drug-induced antibodies should be more considered in combination with medication history to better ensure the safety and effectiveness of blood transfusion.