OBJECTIVE:To analyze the features and general patterns of adverse drug reactions (ADR) occurred in our hospital. METHODS: 209 ADR case reports collected in our hospital in 2007 were analyzed statistically in respect of patients' age and sex, route of administration, category of the drugs, organs and systems involved, and clinical manifestation of the ADRs. RESULTS: The ADR were more likely caused via intravenous dip (129 cases, 61.72%). Anti-infectives had the highest proportion to induce ADR(94 cases,44.98%), followed by nervous system drugs and cardiovascular system drugs. Quinolones were the leading anti-infective drugs causing ADR(35 cases, 37.23%), of which, Levofloxacin dominated the first place. The main presentation of the ADR was damage of skin and its appendages such as erythra and pruritus etc, followed by the lesions of digestive system and circulatory system. CONCLUSION: ADR monitoring awareness should be strengthened and the related ADR reporting system should be improved.

OBJECTIVE:To investigate the clinical feature of drug-induced acute renal failure(ARF).METHODS:285 ARF cases retrieved from CBMdisc and CNKI between Jan.1995 and Dec.2007 were analyzed statistically.RESULTS:Of the 285 drug-induced ARF cases reviewed,186 were male and 99 female.The drugs induced ARF totalled 50 kinds,leading the list in order were mannitol,acyclovir,dopamine,rifampicin,gentamicin,and dextran 40 etc;242(84.91%)received medicine by intravenous drip route.ARF appeared more often from day 1 to 5 after drug use;251 were cured(88.07%),6 took a favorable turn(2.11%),5 discharged from hospital automatically(1.75%),4 developed chronical renal failure(1.40%)and 24 died(8.42%).CONCLUSION:Lots of medicines and common drugs can induce ARF,with incidence rate of drug-induced ARF increased increasingly,however,which has a satisfactory prognosis if with timely and accurate diagnosis and suitable treatment.

OBJECTIVE: To probe into the current status of prescription indicators of outpatients in our hospital.METHODS: Drug utilization in the outpatients between 2005 and 2007 in our hospital was studied retrospectively.RESULTS: Over the three years,the prescription drugs declined from 2.40 to 2.25 kinds;the application ratio of antibacterial drugs declined from 20.74% to 20.25% and its variety has declined from 116 to 110 kinds;the application ratio of injections has declined from 11.84% to 9.48% with variety declined from 304 to 299 kinds.CONCLUSION: The indicators of the mean variety of prescription drugs,the mean variety of anti-infection drugs and injections and their ratios in outpatient department are rational on the whole.Comprehensive and effective interventions are workable and achievable in the promotion of rational drug use.

0. 05). This paper surggests that the activity of Na+-K+ ATPase on erythrocyte membrane may be a indicator in the diagnosis of stroke and human senility. The mechanism of changes of Na+-K+ATPase was also discussed in this paper.

0.05).The percentage of apoptotic lymphocytes in those with more than two kinds of autoantibodies was markedly higher than those with only one kind of autoantibody(P

Objective To clone the coding region and 3′non?coding region of calmodulin 2(CaM2)in guinea pig,to provide the genetic informa?tion for studying the gene function of Calmodulin 2. Methods Total RNA was extracted from heart tissue of guinea pig,the coding region and 3′non?coding region of CaM2 were amplified by RT?PCR and 3′?RACE PCR methods,and the recombinant plasmid was constructed by inserting cDNA of the coding region and 3′non?coding region of CaM2 into the cloning vector by genetic engineering technology followed by DNA sequencing and se?quence analysis. Results The cloned coding region of CaM2 was 450 bp,and the 3′non?coding region of CaM2 was 660 bp. The amino acid se?quences of the coding region of CaM2 was consistent with those of other CaM subtypes,and the 3′non?coding region of CaM2 had low homology with those of other subtypes. Conclusion The cloning of CaM2 coding region and 3′non?coding region in guinea pig was the foundation for further study on the gene function of CaM2 and its role in related diseases.

Objective To set up the rolling circle amplification (RCA) system for detecting hepatitis B virus (HBV) covalently closed circular DNA (cccDNA), and to evaluate the specificity and sensitivity of this system. Methods Plasmids containing full-length of wild-type HBV genome were treated with restriction enzyme and T4 DNA ligase, and then were concentrated. The DNA fragments were recovered by the nucleic acid purification kit and severed as standard HBV cccDNA. Total DNA was extracted from hepatic tissues of seven chronic hepatitis B patients. RCA method was used to amplify genomes from tissue samples. Standard HBV cccDNA, 3.2 kb liner HBV DNA, normal hepatic tissue samples and 15 serum samples of patients with chronic HBV infection were used as controls to determine the specificity of RCA. Ten-fold serial dilutions of standard HBV cccDNA were used for determining the sensitivity. Results The standard HBV cccDNA was successfully constructed and could be detected by RCA method. HBV cccDNA could be amplified from 2 mg hepatic tissue samples at least of HBV infected patients, and could be detected as low as 1 ×102 copy/μL. cccDNA was not detected in 3.2 kb liner HBV DNA, normal hepatic tissue samples and 15 serum samples of chronic HBV infected patients. Conclusion RCA method can be used for rapid and simple detection of HBV cccDNA with high specificity and sensitivity.

Objective To investigate the imaging features of perivascular epithelioid cell tumor of liver (PEComa),and to improve the diagnostic level of the disease. Methods A retrospective analysis of imaging manifestations was performed on 11 cases of hepatic hemangioma confirmed by surgery and pathology. All patients underwent CT and MRI examination before PET/CT examination ,then received operation. Results Eleven cases were solitary hepatic lesions ,10 of which were located in the right lobe of the liver ,and 1 in the left. The lesions were round or quasi-circular and the diameter ranged from about 13.5cm to about 1.8 cm,with an average of 5.7 cm. CT plain scanning showed that the liver was slightly lower density ,with clear boundary. The smaller lesions were homogeneous density,while the larger ones were not,and the lower density area was visible. Enhancement of peripheral lesions in the arterial phase was enhanced. The lesions in the portal vein were not uniformly enhanced , and visible separation was found in large lesions. In the delay phase ,the lesions were significantly decreased or slightly lower. MRI plain scan showed the lesion T1WI showed moderate signal or slightly low signal ,and large lesion signal was not uniform,and T2WI lesions showed slightly high signal while DWI lesions high signal and the enhanced features were similar to those of CT. PET/CT examination showed that visible radioactive uptake of abnormal concentration or uneven concentration were found in lesions with diameter larger than 3.0 cm,and the maximum SUV value ranged from 12.2 to 3.6,with an average value of 5.7. Lesions with diameter less than 3.0 cm showed a slight concentration of radioactive uptake and the highest SUV value was 4.7,with an average value of 2.9. The only 11.8 cm lesion showed no significant radioactivity ,which was as the same as that of normal liver tissue. Delayed PET/CT imaging(1 hour)showed that SUV values of all lesions were decreased in varying degrees. Conclusion PET/CT imaging of perivascular epithelioid cell tumor of liver has its characteristics. The combina-tion of PET/CT imaging and routine imaging examination will benefit the improvement of diagnosis level obviously.

Objective With the focus on emerging infectious diseases and diseases of unknown cause,the study aims to realize multi-point trigger monitoring of infectious diseases through key monitoring sites and key populations.Methods Using ar-tificial intelligence,deep learning,big data and other information technologies to build an intelligent information center for infec-tious diseases with patients'disease files as the core,construct a core capacity of infectious disease surveillance,early warning and situation prediction,and predict and evaluate the importance of infectious disease warning signals.Results The system cov-ered 1 425 primary-level medical institutions,18 hospitals,2 580+schools,4 134 pharmacies,4 laboratories and civil affairs departments,detected 55 kinds of infectious diseases and 6 kinds of syndrome monitoring signals.Since its launch,121 000 ac-tive notification cards have been issued,more than 54 000 new notification cards have been added,35.256 million times of multi-source monitoring and 14.4 million disease files have been recorded.Conclusion By expanding monitoring content and chan-nels,we realized early monitoring,auxiliary investigation and multi-mode visual early warning of infectious diseases,built a multi-point trigger mechanism,and moved forward the infectious disease surveillance.

Aquaporin 9 (AQP9),one of members of the cell membrane protein family,plays an important role in maintaining metabolism and water balance in vivo.AQP9 could also play an importan role in development of tumors,such as migration,metastasis and apoptosis of tumor cells.In addition,AQP9 is of great significance in judging prognosis of tumors.