BACKGROUND:The form and structure of antigen-extracted xenogeneic cancel ous bone through series of physical and chemical treatment are similar to human tissue. OBJECTIVE:To detect the biocompatibility of antigen-extracted xenogeneic cancel ous bone matrix prepared by three different ways. METHODS:The antigen-extracted xenogeneic cancel ous bone scaffold materials which were prepared through physical, chemical and physical-chemical combined methods and hydroxy apatite biological ceramic materials were implanted into the dorsum subcutaneous tissue. Histological observation was done at 4, 8 and 12 weeks after surgery. The antigen-extracted xenogeneic cancel ous bone scaffold materials which were prepared through physical, chemical and physical-chemical combined methods respectively was used to culture sheep bone marrow mesenchymal stem cells for 7 days. Cel adhesion, growth, proliferation and stroma secretion were observed. RESULTS AND CONCLUSION:At 4 weeks after surgery, a strong inflammatory reaction was detected around materials in four groups. At 12 weeks, the xenogeneic bone materials prepared through physical and physical-chemical combined methods and hydroxy apatite biological ceramic materials internal pore and surrounding tissue inflammation disappeared basical y, with the presence of thimbleful inflammation cells. The material degradation was more than at 8 weeks. The xenogeneic bone materials prepared through chemical methods material internal pore and surrounding tissue inflammation stil existed, suggesting that the xenogeneic bone materials prepared through physical and physical-chemical combined methods exhibited good histocompatibility. A smal amount of orderly osteoblasts existed around hydroxy apatite biological ceramic materials and physical-chemical prepared materials, with a smal amount of bone. These suggested that there was a tendency for ectopic bone formation. The xenogeneic cancel ous bone materials prepared through physical or physical-chemical combined methods have better cytocompatibility. However, scaffold materials prepared through chemical method have poor cytocompatibility and they are not qualified for the safety standards of biological materials.

Objective:To study TGC on tumor apopotosis and to apply the experimenting basis of TGC's developing and utilizing.Methods:The flow cytometer(FCM) was used for determination apoptosis.The protein expression of Bcl-2 and the mRNA expression of c-myc were evaluated by immunohistochemistry and in situ hybridization.Apoptosis small body were observed by EU.Results:The TGC group appear the Ap apex,the number of cell of G0-G1 period reduce obviously,the cells of S period increase obviously.Bcl-2 and the c-myc mRNA expression levels were decreased in TGC group.The structure of inner membrane was intacted with EU.Chromatin gathered side of cell,the nucleus turns into the nucleus band and nucleus project,there were many cells of the apoptosis and meniscus or the garland shape(the apoptosis corpuscle formatiol) in TGC group.Conclusion:The total glucosides of radix paeoniae induces the tumor cell apoptosising,its mechanism maybe closely related with adjusting expression levels of the Bcl-2 and the c-myc mRNA.

Objective Experiment the tumor animal model of the H22 Cancer of the liver mile,study the inhibition effect of chitosan oligosaccharide and bifidobacteria on tumour.Methods the growth of tumour were observed after chitosan oligosaccharide and bifidobacteria were injected into tumor-bearing mice;then the immunify funcfion weremcasured.Results Growth of tumour were inhibited by chitosan oligosaccharide and bifidobacteria,they can increase the content of IL-2 and IFN-?and the weight of spleen and thymus of the mice.Conclusions chitosan oligosaccharide and bifidobacteria could inhibit the growth of tumour,inproue immunity function.

Objective To investigate the effects of quercetin on the proliferation of mammary cancer cell in vitro. Methods Effects of quercetin on the cell proliferation was tested in ER-positive MCF-7 cell and ER-negative MDA-MB231 cell by MTT measurement. And to evaluate the estrogen-like effect of quercetin and its relation with the estrogen-receptor by pure estrogen receptor antagonist ICI 182, 780 as a tool. Cell cycle was examined by flow cytometry. Result Quercetin (10～50 ?mol/L) stimulated proliferation of ER-positive MCF-7 cell compared with solvent control, whereas ER-negative MDA-MB231 cell proliferation effect was inhibited, and the cell cycle was impulsed from G1 to S, DNA synthesizing was inhanced, PI was also increased. The above function on boosting MCF-7 cell proliferation can be inhibited by adding estrogen receptor antagonism ICI182, 780. Conclusion Quercetin has the estrogen-like activities through the estrogen response pathway.

Asbestos ; toxicity ; Humans ; Occupational Exposure ; adverse effects ; Pulmonary Alveolar Proteinosis

OBJECTIVE:To optimize the extraction technology of chlorogenic acid in Prunus armeniaca flos,and compare the contents in P. armeniaca flos from different origins and varieties. METHODS:HPLC was conducted to determine the content of chlorogenic acid in P. armeniaca flos;using the volume fraction of ethanol,the ratio of material to liquid,ultrasonic extraction times and time as factor,the content of chlorogenic acid as index,single factor and orthogonal test were designed to optimize the extraction technology,and verification tests were carried out. The optimized extraction technology was used to extract and compare the contents of chlorogenic acid in Armeniaca sibirica from 7 origins of P. armeniaca flos and 3 origins of Armeniaca sibirica flos. RESULTS:The optimized extraction technology was extracting twice with 12-fold 75% ethanol,30 min each time. Under the con-ditions,the content of chlorogenic acid can reach 77.38 mg/g(RSD=0.58%,n=3);the contents of chlorogenic acid in A. sibiri-ca flos and P. armeniaca flos were 77.38-83.33 mg/g and 63.12-70.22 mg/g,respectively. CONCLUSIONS:The established extrac-tion technology is reasonable,stable and feasible. The contents of chlorogenic acid in A. sibirica are higher than that in P. armenia-ca flos;the contents have no obvious differences in the same variety of A. sibirica from different origins.

Objective To connect the content of clinical immunology and inspection with network,so that it is more convenient for medical students to understand and study this course,and the learners’interest in learning is aroused.Methods FrontPage 2003 web page authoring software,as well as animation software(flash) and picture editing software(Photoshop) were used to make a vivid image of the teaching content be shown through the web page,and effectiveness evaluation was done through peer review and students survey about the software.Results Using FrontPage to create web pages and change knowledge into learning pattern.got good results in studetns’self-study,and the"online self-test"section was added,which would more effectively check study effect.There were good student feedbacks.Conclusion Applying this learning pattern to study will play a very good role in deepening students’understanding of curriculum,improving their study efficiency and stimulating their interest in learning.

OBJECTIVETo study the total glucosides of Radix Paeoniae Rubra induced K562 tumor cell apoptosis of signaling pathways and related gene changes.

METHODBy MTT and flow cytometric, real-time quantitatie polymerase chain reaction (PCR) and Western blot methods researched the level of genes and proteins.

RESULTThe total glucosides of Radix Paeoniae Rubra could inhibit K562 cell growth by MTT method, there was the relationship of concentration-time between them, TGC prompted K562 cell translocation of phosphatidylserine, may be apoptosis through non-receptor-dependent pathways because caspase-3 mRNA, caspase-9 mRNA and cytochrome C increased, caspase-8 mRNA had no significant change. The expression of Bcl-2 protein and Bcl-X1 protein could decreased, the expression of Bax protein could increased by regulating gene expression.

CONCLUSIONTGC induced K562 cell apoptosis that might be through the mitochondria pathway, when cell apoptosis occured, Bcl-2 or Bcl-XI proteins combination of Bax protein would be displacement, then the mitochondrial membrane became permeable to release a series of material, then lead to cell death.

Antineoplastic Agents ; pharmacology ; Apoptosis ; drug effects ; genetics ; Caspases ; genetics ; Dose-Response Relationship, Drug ; Gene Expression Regulation, Neoplastic ; drug effects ; Glucosides ; pharmacology ; Humans ; K562 Cells ; Paeonia ; chemistry ; Signal Transduction ; drug effects ; Time Factors

Objective To study the effects of ganoderma applanatum polysaccharides(GAPS) on cell morphology, proliferation and PDGFR -?expression in cell lines MGC - 803 , and to explore its potential mechanisms of anti - tumor of GAPS. Methods Cell morphology was observed by inverting microscope. MTT assay was used to investigate the inhibitory effect of GAPS on MGC -803.Expressions of PDGFR -?was analyzed by flow cytometry (FCW). The fluorescence intensity of expressions of PDGFR -?was observed by fluorescence microscope. Results Cells of GAPS group were irregular shaped and grew poorly. GAPS inhibited the proliferation of MGC -803 cells in dose - dependent and time - dependent manners . After MGC - 803 cells were treated with GAPS for 72h, GAPS could down - regulate expression of PDGFR -?observed by fluorescence microscope which was consistented with the results of FCW with statistic significance difference as compared with control group (P

Fatal Outcome ; Herbicides ; poisoning ; Humans ; Injections, Intravenous ; Paraquat ; poisoning ; Poisoning ; mortality