We have developed an antibody-captured sandwich-type enzyme linked immunosorbant assay(ELISA) with monoclonal anti-IgA/a (human) and monoclonal anti-HBc, and determined immunogloblin A against hepatitis B core antigendgA anti-HBc) in sera of 179 cases of various types of HBV infection. IgAanti-HBc in the patients with acute hepatitis B (14 cases) and severe hepatitis (7 cases) was all positive, while in the patients with chronic active hepatitis, cirrhosis, chronic persistent hepatitis, and symptomatic carriers the positive rates were 94% (47/50 cases), 83.3% (10/12 cases), 56.5%(26/46 cases) and 4%(2/50 cases), respectively. Our observations suggested that IgA anti-HBc may reflect hepatic damage in the patients with HBV infection. Its detection may be valuable in the diagnosis and prognosis HBV infection. In addition, the activity of IgA anti-HBc in chronic hepatitis B is related to HBeAg.

AIM: To investigate 86 patients (age range, 12 to 80 years; mean?SD age 48.63?18.11) who had status epilepticus. Among them 60 patients had generalized tonic-clonic status epilepticus, 21 myoclonic clo-nictonic clonic status epilepticus 3 generatized tonic status epilepti-cus and 2 patients complex partial status epilepti-cus-psychomotor status epilepticus. METHODS: We conducted two centers, of 45 patients were given Sod. Phenobarbital im group and of 41 patients were given Diazepam Ver iv group. RESULTS: Significant difference in control with status epilepticus, (28.53?10.95) min and (33.85?11.53) min, P

Objective To compare the effectiveness of the three operation mode in treatment of ovarian cyst. Methods According to the digital table,138 patients with ovarian cyst were divided into three groups,the group A (n=46 cases)were operated through open operation,group B (n =46 cases)were operated through transvaginal operation,group C(n=46 cases)were operated through laparoscopic operation.The three groups were observed the situation of operation (operation time,bleeding volume,the first exhaust time,hospitalizationtime ),complications (infection,hemorrhage,perimenopausal changes ),postoperative follow -up for 1 years.The recurrence rate was observed.Results Group A of 46 patients were successfully completed surgery,B,C groups of 1 patients was con-versed to laparotomy due to severe adhesion.B,C groups in the operation time,amount of bleeding during the opera-tion,the time of hospitalization were significantly less than that of group A (t=4.306,5.172,3.012,3.926,3.776, 2.168,P<0.05,P<0.01).B group was significantly less than those of A,C group in the first exhaust time (t=3.014,2.446,all P<0.05).In the operation time of group B was obviously less than that of group C (t=2.748,P<0.05).A,B,C three groups of postoperative complication rates were 39.1%,11.1%,15.6% respectively;the incidence of complications of B,C groups was lower than that in A group (χ2 =9.82,8.64,all P<0.01 ).After 1years follow-up,the recurrence rate in group B was significantly higher than that of A and C groups (χ2 =6.72, 6.72,all P<0.01).Conclusion Treatment of ovarian cyst vaginal surgery and laparoscopic surgery wound is small, but the person that weigh should be performed open adhesion treatment,individualized treatment.

Objective To analyze the pathogeny of acute respiration failure after kidney transplantation on early period and discuss the clinical value of mechanical ventilation.Methods A total of 16 cases of acute respiration failure after kidney transplantation on early period were studied retrospectively.Results The main causes were acute pulmonary edema caused by heart failure and serious pneumonia; patients were divided into two groups according to pathogeny: heart failure group (n=8) and non-heart failure group (n=8). It was found that the patients in heart failure group were older, preoperative blood pressure higher, dialysis duration longer, acute respiratory failure occurred earlier and mechanical ventilation time shorter than in non-heart failure group. The mechanical ventilation could raise oxygen pressure in artery blood and correct hypoxemia quickly. The mechanical ventilation could keep oxygen pressure in artery blood on the ideal level.Conclusion The pathogeny of acute respiration failure after kidney transplantation on early period included acute pulmonary edema caused by heart function failure and serious pneumonia; the mechanical ventilation was important to treat patient with acute respiration failure after kidney transplantation on early period because it could ensure oxygen pressure in artery blood in order to win time to cure heart failure and serious pneumonia. Also, the mechanical ventilation could improve prognosis of the patients.

ObjectiveTo explore the effect of motor on functional recovery of the lower limb in hemiplegic patients after cerebral infarction. Methods62 patients recovering from cerebral infarction were divided into the control group (30 cases) and motor imagery group (32 cases). All patients received routine treatment and a motor relearning program. Patients in the motor imagery group were given mental practice in activities of the lower limb. They were assessed with Fugl-Meyer Assessments (FMA) and Modified Barthel Index (MBI) before and after treatment. ResultsThe scores of FMA and MBI were improved in the motor imagery group compared with those in the control group (P<0.05) after treatment. ConclusionMotor imagery can facilitate the recovery of lower limb function in patients with hemiplegia.

BACKGROUND: Due to dentition defect, dentition loss, periodontal disease, trauma and tumor, many patients have to face insufficient buccal-lingual mandibular width. At present, there is no consistent conclusion in suitable peri-implant buccal-lingual mandibular width. OBJECTIVE: To investigate the stress of implant-bone interface with three-dimensional finite element method, in order to evaluate buccal-lingual mandibular width suitable for implants. METHODS: Classes Ⅰ and Ⅲ mandible implant models (the buccal-lingual width of implant neck region was 1, 1.5, 2, 2.5, 3 and 3.5 mm) were loaded with 200 N forces vertical y and at 60° oblique. Then, the stress and strain in the implant-bone interfacial were analyzed. RESULTS AND CONCLUSION: Almost 2 mm or more than 2 mm of mandible bone width could result in good stress distribution in implant-bone interface. The stress distribution of oblique loading was much greater than that of vertical loading. Proper quantity of peri-implant mandibular width is good for stress distribution in implant-bone interface. In the clinical treatment, the oblique loading should be avoided or reduced.

p38 mitogen-activated protein kinase (p38) is a member of MAP kinase family. Its widespectrum roles in the control of energy metabolism have been indicated in numerous studies. P3 8 participates in the energy metabolism in all major tissues/organs involved in the control of energy metabolism, including adipose tissue, skeletal muscles, islet cells, and liver. In white adipose tissue, p38 plays an important role in adipose differentiation and glucose uptake although it is still inconclusive whether this role of p38 is stimulatory or inhibitory. The stimulatory role of p38 in transcription of the uncoupling protein 1 ( UCP1 ) gene in brown adipose tissue is relatively clear. A fundamental role for p38 in the differentiation of skeletal muscles and mitochondrial biogenesis in skeletal muscles is rather definitive although the role of p3 8 in glucose uptake of skeletal muscles remains controversial. In islet cells, p38 appears to be involved in β-cell apoptosis. P38 has been indicated in the control of preproinsulin gene transcription, but remains controversial. However, it seems clear that p38 does not play a significant role in insulin secretion. In the liver, p38 plays a central role in hepatic glucose and lipid metabolism. Activation of p38 participates in the processes to increase blood glucose levels through reducing glycogen synthesis and increasing hepatic gluconeogenesis. P38 appears to prevent fat storage by inhibiting hepatic lipogenesis and promoting fatty acid oxidation in the liver. Additionally, p38 may play a critical role in cholesterol metabolism by regulating expression of the LDLR gene and bile metabolism. P38 does not only participate in various physiological and pathophysiological processes in cardiomyocytes, but also is heavily involved in the development of atherosclerotic lessions through its influences on monocytes/macrophages, vascular endothelial cells, and vascular smooth muscle cells.

BACKGROUND: The conical telescopic crown denture has a better long-term effect on dentition defects of few remaining teeth than the traditional removable denture. However, there are few studies to analyze the application of these two kinds of repair methods using the finite element method. OBJECTIVE: To compare the stress distribution of the isolated abutment tooth repaired using the conical telescopic crown denture and traditional removable denture. METHODS: Two-dimensional images of the mandible and dentition in healthy adults were scanned by CT. Three-dimensional finite element models of conical telescopic crown denture and traditional removable denture were established in the mandible only with the bilateral canines. Under the same vertical and oblique loadings, the maximum equivalent stress of the abutment, periodontal membrane or edentulous alveolar ridge was compared between two repair methods. RESULTS AND CONCLUSION: Under the vertical and oblique loadings, the total stress of abutment and periodontal membrane in the traditional removable denture was higher than that in the conical telescopic crown denture and the stress in the corresponding ring was concentrated obviously; the total stress of edentulous alveolar ridge in the conical telescopic crown denture was slightly larger than that in the traditional removable denture, but the stress was wel -distributed and concentrated in a smal area. Under the oblique loading, the total stress of abutment, periodontal membrane and edentulous alveolar ridge was higher than that under the vertical loading in these two dentures, and the stress was concentrated in the periodontal membrane of lingual neck, but the total stress in the traditional removable denture was larger, with a wider concentration range. These findings indicate that the stress on the supporting tissue of conical telescopic crown denture is more evenly distributed within a smal er range than that of traditional removable denture, and the former is more fit for the health of abutment, periodontal tissues and edentulous alveolar ridge.

BACKGROUND: Hydroxyapatite/β-tricalcium phosphate biphasic ceramic bone has good cel compatibility, but its mechanical properties are poor. OBJECTIVE: To construct chitosan/ or calcium alginate/biphasic ceramic bone scaffolds and to detect their mechanical properties and cytocompatibility. METHODS: Different concentrations of chitosan (2%, 4%, 7%, 10%) or calcium alginate (3%, 4%, 5%, 7%) were mixed with biphasic ceramic bone to prepare chitosan/biphasic ceramic bone scaffold and calcium alginate/biphasic ceramic bone scaffold. Their morphology and structure, coagulation time, anti-dissolution properties, shear force, compressive strength and cel compatibility were detected. RESULTS AND CONCLUSION: (1) Coagulation time: with the concentration increase, the initial and final setting time of these two kinds of composite scaffolds were prolonged to some extent. (2) Scanning electron microscopy: these two kinds of composite scaffolds showed porous microstructures with different pore sizes. (3) Anti-dissolution properties: the calcium alginate/biphasic ceramic bone scaffold (3%, 4%, 5%, 7%) and chitosan/biphasic ceramic bone scaffold (7%, 10%) had good anti-dissolution properties in the liquid. (4) Mechanical strength: with the concentration increase, the shear force and compressive strength of the calcium alginate/biphasic ceramic bone scaffold were reduced. (5) Cel compatibility: the cytotoxicity of chitosan/ or calcium alginate/biphasic ceramic bone scaffolds was graded as 0-1 or 2-3, respectively. These results show that the chitosan/biphasic ceramic bone scaffold has better mechanical properties and cel compatibility than the calcium alginate/biphasic ceramic bone scaffold.

Objective To report the result of analysis of 72 cases of facial cutaneous sporotrichosis. Methods Seventy-two patients were included in this study. They were diagnosed as facial cutaneous sporotrichosis according to clinical mainfestations and fungal culture. Histopathological examinations were done in some atypical cases. The patients were treated with combined antifungal agents. Results The lesion forms were varied, in which lymphangitic cutaneous form accounted for 9.72% and fixed cutaneous form accounted for 90.28% respectively. Nineteen cases (26.4%) had a history of skin trauma before the cutaneous lesions. All of the patients were successfully treated with potassium iodide and/or itraconazole, terbinafine for 6-8 weeks. Conclusions The skin lesions and mycological examination are the important evidences for the diagnosis of sporotrichosis. The main form of the facial cutaneous sporotrichosis is the fixed cutaneous form. The misdiagnosis rate is 16% in primary level clinics. Potassium iodide is an effective and inexpensive medicine in the treatment of cutaneous sporotrichosis.