AIM: To investigate 86 patients (age range, 12 to 80 years; mean?SD age 48.63?18.11) who had status epilepticus. Among them 60 patients had generalized tonic-clonic status epilepticus, 21 myoclonic clo-nictonic clonic status epilepticus 3 generatized tonic status epilepti-cus and 2 patients complex partial status epilepti-cus-psychomotor status epilepticus. METHODS: We conducted two centers, of 45 patients were given Sod. Phenobarbital im group and of 41 patients were given Diazepam Ver iv group. RESULTS: Significant difference in control with status epilepticus, (28.53?10.95) min and (33.85?11.53) min, P

We have developed an antibody-captured sandwich-type enzyme linked immunosorbant assay(ELISA) with monoclonal anti-IgA/a (human) and monoclonal anti-HBc, and determined immunogloblin A against hepatitis B core antigendgA anti-HBc) in sera of 179 cases of various types of HBV infection. IgAanti-HBc in the patients with acute hepatitis B (14 cases) and severe hepatitis (7 cases) was all positive, while in the patients with chronic active hepatitis, cirrhosis, chronic persistent hepatitis, and symptomatic carriers the positive rates were 94% (47/50 cases), 83.3% (10/12 cases), 56.5%(26/46 cases) and 4%(2/50 cases), respectively. Our observations suggested that IgA anti-HBc may reflect hepatic damage in the patients with HBV infection. Its detection may be valuable in the diagnosis and prognosis HBV infection. In addition, the activity of IgA anti-HBc in chronic hepatitis B is related to HBeAg.

Objective To compare the effectiveness of the three operation mode in treatment of ovarian cyst. Methods According to the digital table,138 patients with ovarian cyst were divided into three groups,the group A (n=46 cases)were operated through open operation,group B (n =46 cases)were operated through transvaginal operation,group C(n=46 cases)were operated through laparoscopic operation.The three groups were observed the situation of operation (operation time,bleeding volume,the first exhaust time,hospitalizationtime ),complications (infection,hemorrhage,perimenopausal changes ),postoperative follow -up for 1 years.The recurrence rate was observed.Results Group A of 46 patients were successfully completed surgery,B,C groups of 1 patients was con-versed to laparotomy due to severe adhesion.B,C groups in the operation time,amount of bleeding during the opera-tion,the time of hospitalization were significantly less than that of group A (t=4.306,5.172,3.012,3.926,3.776, 2.168,P<0.05,P<0.01).B group was significantly less than those of A,C group in the first exhaust time (t=3.014,2.446,all P<0.05).In the operation time of group B was obviously less than that of group C (t=2.748,P<0.05).A,B,C three groups of postoperative complication rates were 39.1%,11.1%,15.6% respectively;the incidence of complications of B,C groups was lower than that in A group (χ2 =9.82,8.64,all P<0.01 ).After 1years follow-up,the recurrence rate in group B was significantly higher than that of A and C groups (χ2 =6.72, 6.72,all P<0.01).Conclusion Treatment of ovarian cyst vaginal surgery and laparoscopic surgery wound is small, but the person that weigh should be performed open adhesion treatment,individualized treatment.

Objective To analyze the pathogeny of acute respiration failure after kidney transplantation on early period and discuss the clinical value of mechanical ventilation.Methods A total of 16 cases of acute respiration failure after kidney transplantation on early period were studied retrospectively.Results The main causes were acute pulmonary edema caused by heart failure and serious pneumonia; patients were divided into two groups according to pathogeny: heart failure group (n=8) and non-heart failure group (n=8). It was found that the patients in heart failure group were older, preoperative blood pressure higher, dialysis duration longer, acute respiratory failure occurred earlier and mechanical ventilation time shorter than in non-heart failure group. The mechanical ventilation could raise oxygen pressure in artery blood and correct hypoxemia quickly. The mechanical ventilation could keep oxygen pressure in artery blood on the ideal level.Conclusion The pathogeny of acute respiration failure after kidney transplantation on early period included acute pulmonary edema caused by heart function failure and serious pneumonia; the mechanical ventilation was important to treat patient with acute respiration failure after kidney transplantation on early period because it could ensure oxygen pressure in artery blood in order to win time to cure heart failure and serious pneumonia. Also, the mechanical ventilation could improve prognosis of the patients.

ObjectiveTo explore the effect of motor on functional recovery of the lower limb in hemiplegic patients after cerebral infarction. Methods62 patients recovering from cerebral infarction were divided into the control group (30 cases) and motor imagery group (32 cases). All patients received routine treatment and a motor relearning program. Patients in the motor imagery group were given mental practice in activities of the lower limb. They were assessed with Fugl-Meyer Assessments (FMA) and Modified Barthel Index (MBI) before and after treatment. ResultsThe scores of FMA and MBI were improved in the motor imagery group compared with those in the control group (P<0.05) after treatment. ConclusionMotor imagery can facilitate the recovery of lower limb function in patients with hemiplegia.

p38 mitogen-activated protein kinase (p38) is a member of MAP kinase family. Its widespectrum roles in the control of energy metabolism have been indicated in numerous studies. P3 8 participates in the energy metabolism in all major tissues/organs involved in the control of energy metabolism, including adipose tissue, skeletal muscles, islet cells, and liver. In white adipose tissue, p38 plays an important role in adipose differentiation and glucose uptake although it is still inconclusive whether this role of p38 is stimulatory or inhibitory. The stimulatory role of p38 in transcription of the uncoupling protein 1 ( UCP1 ) gene in brown adipose tissue is relatively clear. A fundamental role for p38 in the differentiation of skeletal muscles and mitochondrial biogenesis in skeletal muscles is rather definitive although the role of p3 8 in glucose uptake of skeletal muscles remains controversial. In islet cells, p38 appears to be involved in β-cell apoptosis. P38 has been indicated in the control of preproinsulin gene transcription, but remains controversial. However, it seems clear that p38 does not play a significant role in insulin secretion. In the liver, p38 plays a central role in hepatic glucose and lipid metabolism. Activation of p38 participates in the processes to increase blood glucose levels through reducing glycogen synthesis and increasing hepatic gluconeogenesis. P38 appears to prevent fat storage by inhibiting hepatic lipogenesis and promoting fatty acid oxidation in the liver. Additionally, p38 may play a critical role in cholesterol metabolism by regulating expression of the LDLR gene and bile metabolism. P38 does not only participate in various physiological and pathophysiological processes in cardiomyocytes, but also is heavily involved in the development of atherosclerotic lessions through its influences on monocytes/macrophages, vascular endothelial cells, and vascular smooth muscle cells.

Objective To explore the effect of antenatal taurine supplementation on cerebral apoptosis and the expression of glial cell line-derived neurotrophic factor (GDNF) and caspase-3 in fetal rats with fetal growth restriction (FGR).Methods Fifteen pregnant Sprague-Dawley rats were randomly divided into three groups:control group,FGR model group (model group) and FGR with antenatal taurine supplementation group (taurine group).Taurine was added into the diet of taurine group at a dose of 300 mg/(kg · d) from the 12th day of gestation until natural delivery.Two appropriate for gestational age (AGA) newborn rats were randomly selected from each mother in control group and two FGR fetal rats were randomly selected from each mother both in model and taurine groups.Apoptosis of neural cells in the brain was detected by terminal deoxynucleotidyl transferase mediated nick end labeling (TUNEL).Changes in protein expression of GDNF and caspase-3 were detected by immunohistochemistry.Levene method,one-way ANOVA and SNK-test,or Kruskal Wallis rank sum test and Tamhane’ s test were applied for statistical analysis.Results (1)The total amounts of fetal rats in control group,model group and taurine group were 65,60 and 59.The mean body weight of fetal rats were (6.36±0.44) g,(4.55 ± 0.45) g and (5.11±0.67) g,respectively.All fetal rats developed FGR in model group,while 76.3％(45/59) of fetal rats were FGR in taurine group.Therefore,FGR model was successfully established.(2) In control group,there were few expression of TUNEL positive cells in cerebral cortex.A large amount of TUNEL positive cells were found in the cortex,hippocampal and white matter area in model group,but less positive cells were identified in taurine group than in model group.The amount of apoptotic brain cells in the three groups were (0.46 ± 0.11),(14.76 ± 3.42) and (6.78 ± 1.93),respectively(H=429.80,P=0.000).(3)There were only small amount of GDNF positive cells in cerebral cortex in control group and more in model group.The amount of GDNF positive cells was further increased in taurine group.The amount of GDNF positive cells in cerebral cortex in the three groups were (93.56± 6.73),(120.36± 6.23)and(139.56± 5.28),respectively (H=715.17,P=0.000).(4) Few caspase-3 positive cells were found in cerebral cortex in control group.A large amount of positive cells were found in model group and less positive cells were found in taurine group,which was still more than those in control group.The amounts of caspase-3 positive cells in the three groups were (7.50±2.31),(151.32±24.43)and(37.28±11.21),respectively (F=132.54,P=0.000).Conclusions The number of apoptotic neural cells in brain tissue of baby rats with FGR were significantly increased,which can be significantly alleviated by maternal antenatal taurine supplementation through upregulation of GDNF and downregulation of caspase-3 expression.

BACKGROUND: Due to dentition defect, dentition loss, periodontal disease, trauma and tumor, many patients have to face insufficient buccal-lingual mandibular width. At present, there is no consistent conclusion in suitable peri-implant buccal-lingual mandibular width. OBJECTIVE: To investigate the stress of implant-bone interface with three-dimensional finite element method, in order to evaluate buccal-lingual mandibular width suitable for implants. METHODS: Classes Ⅰ and Ⅲ mandible implant models (the buccal-lingual width of implant neck region was 1, 1.5, 2, 2.5, 3 and 3.5 mm) were loaded with 200 N forces vertical y and at 60° oblique. Then, the stress and strain in the implant-bone interfacial were analyzed. RESULTS AND CONCLUSION: Almost 2 mm or more than 2 mm of mandible bone width could result in good stress distribution in implant-bone interface. The stress distribution of oblique loading was much greater than that of vertical loading. Proper quantity of peri-implant mandibular width is good for stress distribution in implant-bone interface. In the clinical treatment, the oblique loading should be avoided or reduced.

The purpose of this study is to evaluate the interaction effects of In-Chen-How (Artemisia capillaries Thunb.) on the pharmacokinetics of acetaminophen and on liver microsomal cytochrome P450 enzyme activity in rats. The rats were divided into control group (n=8) without In-Chen-How and the pretreated group (n=8) administered with In-Chen-How (approximately 1.0 mL·kg-1, according to weight) for 5 consecutive days. Rats in the control group received water simultaneously. Each rat was then given acetaminophen. The pharmacokinetic parameters of acetaminophen of the two groups were significantly different. In the In-Chen-How pretreated group, the maximum concentration of acetaminophen and the area under the plasma concentration-time curve were reduced about 58.4%, 56.7% and 55.4%. To further explain the results, liver microsomal suspensions were obtained from rats that were randomly divided into control and In-Chen-How pretreated group. The levels of CYP1A2 and CYP2E1 in hepatic microsomal protein from pretreated group were increased as compared to that from the control group. It indicated that In-Chen-How can stimulate the activity of CYP isozymes. The changes in the pharmacokinetics of acetaminophen resulting from the administration of In-Chen-How are related to an increase in metabolic activity of CYP1A2 and CYP2E1.

Objective To analyze results of bacterial infection distribution and drug sensitivity monitoring in 414 patients with open extremity injuries caused by Wenchuan earthquake so as to provide advices for guiding utility of antibiotics in treatment of patients injured in natural disasters. Methods Bacterial culture of wound secretion substance and drug sensitivity monitoring were done in 414 patients with open extremity injuries. Results There were 12 patients (2.9%) with G+ coccobacteria, 66 (15.9%) with G- bacillus, 2 (0.5%)with G+ bacillus, 6 (1.5%) with multiplicitas infection(5 with dual infection and I with triplication infection). Main pathogenic bacteria were as follows: (1)G+cocco- bacteria including Staphylococcus epidermidis, Staphylococcus aureus and anthropo-staphylococci ; (2) G- bacillus including Aerobacter cloacae, banmanii, escherichia co]i, aerugo pseudomonas, proteusvul- gaffs and Ewingella americana. (3)G+ bacillus including bacillus subtilis. The drugs with high sensi0tivi- ty to G+ coccobacteria included vancomycin, rifampin, levofloxacin and cidomycin, but those with low sensitivity to G+coccobacteria included penicillin, ampicillin and erythremycin. The drugs with high sen- sitivity to G- bacillus included imipenem, amikacin, levofloxaciu and tenebrimycin, but those with low sensitivity to G-bacillus included ampicillin and piperacillin. Conclusions Patients with open ex- tremity injuries caused by earthquake have rather high bacterial infection rate, involving many kinds of in- fection strains. Therefore, we should do bacterial culture and drug sensitivity monitoring of wound excre- tion substance as early as possible to guide rational use of antibiotics.