Objective To prepare recombinant human adenovirus type 3 expressing Norovirus cap-sid protein gene(Noro-orf2). Methods The cDNA for Noro-orf2 was amplifed by RT-PCR from stool of in-fantile gastroenteritis and cloned into the adenovirus shuttle vector pBSE3CMV-egfp. The vector pBSE3CMV-Nor was linearized with EeoR Ⅴ and Not Ⅰ, and transformed into E. coil BJ5183 with lined edenovirus ge-nomic DNA pLasmid pBRAdv3 by Rsr Ⅱ. The identification of recombinant adenovirus plasmid pBRAdv3E3dNor was performed by PCR, enzyme digestion and DNA sequencing. Then pBRAdv3E3dNor was digested with AsiS Ⅰ and transfeeted into Hep-2 cells with LipofectAMINETM 2000 to package recombi-nant adenovirus particles. Results Noro-orf2 was successfully inserted into the shuttle vector. The recombi-nant adenoviral plasmid pBRAdv3E3dNor was generated by homologous recombination in E. coil BJ5183 and confirmed by PCR and enzyme digestion. The recombinant adenovirus was successfully packaged and puri-fied. Norovirus eapsid protein gene expression was confirmed in Hep-2 cells by immunecytochemistry assay. Conclusion The recombinant type 3 adenovirus expressing Norovirus eapsid protein gene was successfully constructed. This study laid a foundation for developing vaccine against Norovirus.

Objective:To discuss the effect of experiential diet education on the compliance of low-protein diet in patients with chronic kidney disease(CKD), provide basis for improving the compliance of low protein diet in patients with CKD.Methods:A total of 119 patients with CKD stage 3 who were hospitalized from March 2018 to September 2019 in the Division of Nephrology of Beijing Anzhen Hospital, Capital Medical University were selected. They were divided into the following two groups according to the admission time: the control group (58 cases) from March to December 2018 received traditional education, and the observation group(61 cases) from January to September 2019 received systematic experiential diet education on the basis of the control group. At the time of discharge, the eating experience of the observation group was investigated. Three months after discharge, the LPD compliance of the patients in the two groups were investigated by questionnaire, and statistical analyses were performed. And the reason why the observation group could not continue to use CKD nutrition package was analyzed.Results:The score of compliance was (49.44±6.38) points in the observation group and (45.50±6.45) points in the control group, the difference was statistically significant ( t value was 2.68, P<0.05). At the time of discharge, the satisfaction of the observation group was more than 70%, but only 6 patients (9.8%,6/61) adhered to the nutritional package after 3 months. The main reasons why 55 patients could not insist were expensive cost and inconvenient production. Conclusions:Experiential diet education for patients with CKD is a brand-new education model combining routine education and diet experience practice. It can effectively improve the compliance of patients with LPD, which is worth being popularized.

Objective:To investigate the level and change of compliance of functional exercise in patients with breast cancer in one year after operation.Methods:The compliance of limb functional exercise in 90 patients with breast cancer at 3, 6 and 12 months after operation was measured by the compliance scale of functional exercise after operation of breast cancer. Taking time as independent variable( x), functional exercise compliance and index of each dimension as dependent variable( y), regression curve estimation equation was used to draw compliance curve. Results:The functional compliance index of patients at 3, 6, 12 months after surgery was 67.31±14.21, 62.93±12.53, 56.62±14.75, there was significant difference( F value was 31.13, P<0.01). The physical exercise compliance index of patients at 3, 6, 12 months after surgery was 63.06±17.69, 58.03±14.86, 50.83±16.75, there was significant difference ( F value was 30.82, P<0.01). The active recommendation index of patients at 3, 6, 12 months after surgery was 58.56±19.75, 48.56±17.94, 42.94±18.69, there was significant difference( F value was 4.99, P<0.05). The post-consideration index of patients at 3, 6, 12 months after surgery was 81.95±12.70, 83.30±13.25, 78.10±16.55, there was significant difference ( F value was 38.26, P<0.01). The compliance curve of the functional exercise compliance score was y=50.22-1.08 x-0.69 x2 ( R2 value was 0.091, F value was 13.426, P<0.01); the body exercise compliance curve formula was y=23.73-0.64 x-0.39 x2 ( R2 value was 0.086, F value was 12.522, P<0.01); the actively seeking the recommendation compliance curve formula was y=11.67-2.652 x+0.35 x2 ( R2 value was 0.106, F value was 15.911, P<0.01); the postoperative cautionary compliance curve formula was y=14.82+2.22 x-0.65 x2 ( R2 value was 0.023, F value was 3.188, P<0.05). The functional compliance of breast cancer patients during the first year after operation was moderate, and then showed a downward trend. Among them, physical exercise compliance and active seeking compliance showed a low level, and a downward trend. But the postoperative attention complied with a high level. And a trend of a small increase after 6 months after surgery. Conclusions:According to the compliance curve, the overall compliance of postoperative functional exercise in breast cancer patients is poor, and the compliance has a general downward trend with time. This may be related to the low attention of patients, family members and health care workers after discharge or the patient′s own "forgotten curve".Therefore, after discharge, the nurse needs to repeatedly strengthen the patient′s memory, and follow up and supervise the patient according to the key points of the patient′s functional exercise and the reasons for the decline of compliance.

Objective:To verify the capability of small intestinal capsule endoscopy with intelligent assistant system based on deep convolutional neural network (DCNN) in the identification and diagnosis of small intestinal bleeding.Methods:A total of 158 235 small intestinal capsule endoscopy images of 1 970 patients were collected from ESView platform (including 3 765 images of 165 patients with small intestinal bleeding) for training of DCNN-based small intestinal capsule endoscopy with intelligent assistant system. In the validation phase, the capability of the system in identification and diagnosis of small intestinal bleeding was verified by images of 100 patients with small intestinal bleeding (10 cases of active bleeding, 31 cases of blood clot and 59 cases of submucosal hemorrhage).Results:Small intestinal bleeding lesions could be identified by the DCNN-based intelligent assistant system, and also could be displayed with mark on the original capsule endoscopy images. This system also could mark multiple bleeding images of the same lesion and multiple different bleeding lesions in the same image. With this system the average reading time of 100 cases of small intestinal bleeding of images the doctors used was (5.23±1.31) minutes per case. The sensitivity of the diagnosis of small intestinal bleeding was 99.00% (95% confidence interval 93.76% to 99.95%).Conclusions:The sensitivity of small intestinal bleeding identification by small intestinal capsule endoscopy with DCNN-based intelligent assistant system is high, which can be used to assist image reading doctors to identify and diagnose of small intestinal bleeding.

CRISPR-Cas Systems ; Cell Survival ; Genetic Loci ; genetics ; Genome ; genetics ; HEK293 Cells ; Humans ; Luminescent Proteins ; analysis ; Optical Imaging ; methods

From December 2019, the new coronavirus pneumonia (COVID-19) broke out in Wuhan, Hubei, and spread rapidly to the nationwide. On January 20, 2020, the National Health Committee classified COVID-19 pneumonia as one of B class infectious diseases and treated it as class A infectious disease. During the epidemic period, the routine diagnosis and treatment of tumor patients was affected with varying degrees. In this special period, we performed the superiority of the multi-disciplinary team of diagnosis and treatment, achieved accurate diagnosis and treatment of patients with hepatobiliary malignant tumors, provided support for these patients with limited medical resources, and helped them to survive during the epidemic period.On the basis of fully understanding the new coronavirus pneumonia, the treatment strategy should be changed timely during the epidemic, and more appropriate treatment methods should be adopted to minimize the adverse effect of the epidemic on tumor treatment.