Objective To observe the effect of ganglioside GM3 on the proliferation and cell cycle of multiple myeloma (MM) cell line U266. Methods The experimental groups were treated with 20, 40, 80, 160μmol/L GM3, while the control group was not given GM3. The growth inhibition effect of GM3 on U266 cells after 48 hours were measured by using methyl thiazolyl tetrazolium (MTT) method. The cell cycle was tested by flow cytometry with PI labeling. Results The results indicated that the GM3 displayed anti-proliferative effects on U266 cells in a dose-dependent manner. The cell inhibition rates were (13±4) %, (26± 4) %, (47 ±6) %, (55 ±10) % in different dose of GM3 (20, 40, 80, 160 μmol/L), respectively. There was a difference between the experimental group and the control group (F= 93.063, P< 0.05). At the same time, the cell cycle analysis results showed that the U266 cell ratio in S phase was increased from (22.6 ±3.7) % to (71.5±3.8) %(P<0.01) and in G2/M phase was decreased from (42.6±2.5) %to (0.8±0.6) %(P<0.05) while in G0/G1 phase was not significantly changed. Conclusion GM3 can inhibit the proliferation of MM cell line U266 by blocking them in the S phase.

Objective To find the changes and effects of nitric oxide synthase activity in atherosclerotic rabbit models. Methods Thirty rabbits were divided into 3 groups at random: control group, atherogenic diet group, balloon-injury+ atherogenic diet group. Three months later underwent pharmacological triggering with Chinese Russell’s viper venom and histamine to make the plaque rupture. Lipid concentrations and the levels of nitric oxide (NO), NOS and hydroxy radical (OH) in the serum were obtained at different period, and pathologic changes were observed. Results In atherogenic diet group and balloon-injury+ atherogenic diet group, serum lipid concentrations markedly increased, but the levels of NO did not show any difference. The activity of NOS and OH levels were obviously increased after the atherogenic diet. But it reduced after pharmacological triggering and plaque rupture. Conclusion In early period of atherosclerosis, NOS activity up regulated and endothelium functions were compensated. But, When the atherosclerotic plaques were unstable, the activity of NOS descended obviously and endothelium function goes into incompensation.

OBJECTIVE To avoid the hospital(infection) in the dental surgery operating room.METHODS The(international) ISO quality standard was applied to establish the quality control the hospital infection.RESULTS The quality control for the hospital infection was guaranteed in the dental surgery operating room,and the items (observed) were in the allowance of the legislations for the hospital infection.Zero case of hospital infection(happened.) CONCLUSIONS It is useful for the hospital infection administration in the dental surgery operating room to apply the international ISO quality standard.

Objective To construct 2 recombinant plasmids carrying cassette chromosome recombinase C(ccrC)and ccrAB respectively and introduce the plasmids into methicillin-resistant Staphlococcus aureus(MRSA)strain 81/0432,and to observe the precise excision of Staphylococcal cassette chromosome mec(SCCmec)island from bacterial chromosome.Methods ccrC and ccrAB genes were amplified with chromosomal DNAs isolated from MRSA strains 81/0342 and N31S as PCR templates.We constructed recombinant plasmids pSR5C and pSR2AB by cloning ccrC and ccrAB genes into temperature-sensitive plasmid pYI3,after introducing them into MRSA strains 81/0432 and N315 by electroporation.PCR was performed to identify SCCmec excision from the bacterial chromosome.The transformants were serial passaged for 10 days,and then the drug resistance of these rransformants was detected by replica experiment.Results The fragment length of ccrC gene was 1.9 kb,smaller than the fragment length of ccrAB from N315.The recombinant plasmids of pSR5C and pSR2AB were successfully constructed.After these 2 recombinant plasmids were introduced into MRSA strain 81/0342,type-V SCCmec island was excised from the chromosome and formed a closed circular structure in the bacteria.However,type-Ⅱ SCCmec island could be excised only in N31S strain after the expression of pSR2AB.Replica experiment verified that transformed strains of 0342(pSR2AB),0342(pSR5C),and N315 (pSR2AB)were mostly susceptible to ceftizoxim or tobramycin after the excision of SCCmec island.Conclusion cciC could serve as a recombinase as ccrAB,which could mediate precise excision of SCCmec island from the chromosome of type-V MRSA strain.This study shows that type-V SCCmec island is widely disseminated between Staphylococcus aweus strains in community setting.

Objective To investigate the changes of the activity of blood coagulation and fibrinolysis in fracture patients,and to research the relations between the activity and thrombossis. Methods This reser ch chose 60 patients in the department of orthopaedic trauma in our hospital and 10 normal control.Each patient enderwent laboratory analysis on preoperation?post operation 24 hours?post operation 4 days.The molecule markers of coagulation and fibrinolysis TAT(thrombin/antithrombin Ⅲ complex)?F1+2(prothrombin fragment F1+2)?PAP(Plasmin/? 2-antiplasmin complex) were quantitative measured by enzyme immunoassay. Result Compare with normal control,F1+2 and TAT levels increase obviously(P0.05); FG levels increase obviously (P0.05) .Conclusion It has a high level of the activity of blood coagulation and has no obviously change of the activity of fibrinolysis after trauma injury,it indicates high coagulation activity is correlated with thrombossis.

Objective: To observe the acute toxicity of Ke'erxing Capsules (KC) in mice and its long-period toxicity in rats. Methods: The maximal tolerance dose was tested by gastric infusion of single oral dose of KC. Three groups of rats (in high, moderate and low dosage of KC) were administered with repeated gastric infusion of KC for 8 weeks. Body weight, hematological and hematobiochemical parameters, coefficient and histomorphological figure of organs (the heart, liver, spleen, lungs, kidneys, adrenals, testes, prostate, pancreas, uterus, brain, thymus) were examined in 8 weeks and 2 weeks after the cessation of KC. Results: The oral maximal tolerance dose of KC in mice was more than 4000 mg/kg (equal to 600 times of clinical dose). General conditions were changed and the body weight was increased slowly in high-dosage group. The coefficient of prostate was increased in high-dosage group and moderate-dosage group and recovered to normal 2 weeks after stopping the drug. Conclusion: KC has a certain effect on mice and rats but the effect was reversible.

Objective To investigate the effects of recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF)on the gingival wound healing, and to lay a foundation for its application in the field of oral cavity.Methods 16 healthy rabbits were randomly divided into experiment and control groups(n=8).2 mm upper and lower incisors’labial gingivae were removed by scalpel. After operation, rhGM-CSF gel was applied to the surface of the wound gum in experiment group,while saline instead of drugs to the control group.2 rabbits were executed at 3,7,11,and 15 d after operation.HE staining was performed to observe the epithelium and epithelial connective tissue structure, distribution, and the changes of various cells of the rats;the number of positive epithelial cells and fibroblasts were observed by PCNA dyeing.Results On the 3rd day,the inflammatory cell infiltration was found in two groups, but a greater number of the cells were observed in experiment group;neovascularization was seen in both groups on the 7th day,and the fibroblasts and collagen fibers were observed too,but the extent of neovascularization in experiment group was more significant. On the 11th day, the fibroblasts proliferation was seen in two groups,but the extent in experiment group was more widely;on the 15th day,the trauma of gingivae of the rabbits in two groups returned to normal,there was no significant difference between two groups.Over time,the number of epithelial cells in proliferation was gradually increased,reached the peak on the 15th day.The number of fibroblasts began to increase from the 3rd day and reached the peak on the 11th day,and significantly reduced to the lowest value on the 15th day.The number of proliferative epithelial cells in experiment group was significantly higher (P<0.01)on the 3rd day and the 11th day than those in control group (P<0.01);the number of proliferative fibroblasts was significantly higher (P<0.05)on the 11th day and the 15th day than those in control group (P<0.05 ), but there were no significantly statistical differences between two groups at other time pionts(P>0.05).Conclusion RhGM-CSF can promote the infiltration of inflammatory cells, angiogenesis,proliferation of epithelial cells and fibroblasts in gingivae. RhGM-CSF is beneficial with wound healing on gum tissue.

Objective To evaluate the value of retrospective ECG-gated 256 slices spiral CT technique in the scanning of inferior phrenic artery (IPA).Methods 80 patients with underdone abdominal CTA were divided randomly into two groups (40 patients each)as regular abdominal CTA scanning group and retrospective ECG-gated technique group.And further analysis was done to evaluate the display of IPA branches and the image quality.Results 80 patients were all scanned successfully.40 cases LIPA and 40 cases RIPA were showed in the regular group.80 cases showed 1st level branches of IPA,73 cases with 2nd level,59 cases with 3rd and 43 ca-ses showing 4th level.40 cases LIPA and 40 cases RIPA were showed in ECG-gated group.And the numbers of IPA branches levels were 80,79,71,65,respectively.There is no significant difference in the ability of showing the1st level IPA or their image quality between those two groups (P >0.05);and there is a statistical significance in the ability of showing other levels of IPA and their im-age quality.Conclusion It is feasible that we use retrospective ECG-gated 256 slices spiral CT scanning technique to show the IPA. And it could improve the ability of showing the IPA branches as well as the image quality.

This paper introduces the ways to conduct the fixed denture teaching in prosthodontics with the multivariate teaching and learning mode,including information technology and PBL teaching,simulation model system,knowledge integration and the increasing of clinical practice.

Objective To investigate the roles of dCK Ser-74 in radiation-induced cell death in breast cancer cells.Methods Different phenotypes of dCK plasmids were transfected into MCF-7 cells by liposome transfection,including dCK-Vector,dCK-WT (wild type),dCK-S74A (non-phosphorylation) and dCK-S74E (hyper-phosphorylation).All these cells were irradiated by 0,2,4,6,8 Gy X-rays,respectively.The transcriptional and translational level of dCK were detected with real time-PCR and Western blot,respectively.Radiosensitivity was analyzed using cell counting kit (CCK-8) and colony formation assays.Monodansylcadaverine staining (MDC) and flow cytometry were used to detect autophagy and apoptosis,respectively.Results Four phenotypes of dCK cell models were established successfully.After irradiation,the cell viabilities of MCF-7 and dCK-Vector decreased significantly as compared with mock group (t =14.469 and 9.357,P ＜ 0.05),the cell viabilities of dCK-WT,dCK-S74A and dCK-S74E showed no changes (P ＞ 0.05).The total mortalities of dCK-WT and dCK-S74E decreased significantly as compared with dCK-Vector (x2 =3.857-3.971,P ＜ 0.05),but no changes in dCK-S74A cells (P ＞0.05).The apoptosis rates in dCK-S74A,dCK-Vector and control group were up-regulated after irradiation (t =-4.531,-3.688 and-7.076,P ＜ 0.05),and the irradiation-induced apoptosis was reversed in dCK-WT and dCK-S74E (66％ and 68％ of the increase level in dCK-Vector group).The autophagy in dCK-WT and dCK-S74E increased by 22％ and 26％ (t =-9.051 and-8.411,P ＜0.01),but no changes were observed in dCK-S74A,dCK-Vector and control groups (P ＞ 0.05).Conclusions The dCK-WT and dCK-S74E could reverse the irradiation-induced apoptosis,increase the autophagy occurence,and decrease the total mortality,indicating that the phosphorylation of dCK at Ser-74 sites is related to the radiosensitivity of MCF-7 cells.