In this paper, from the current situation of the domestic use of superconducting MRI, on liquid helium supply and demand crisis in the market, the significance of the transformation without LHe of the superconducting MRI magnet was explained, and according to the enterprise's production process, a number of operating without liquid helium transformation practice and ideas were raised, important value orientation for the domestic manufacture and use of superconducting MRI was provided.
Equipment Design ; Magnetic Resonance Imaging ; instrumentation ; Magnetics ; instrumentation

Chronic hepatitis B virus (HBV) infections remain a major public health problem worldwide, which puts human at high risks of liver cancer and liver cirrhosis.The stable nuclear covalently closed circular DNA (cccDNA) is an important step of the HBV replication cycle, as well as the key of drastically destroy the HBV.However, α-interferons and oral nucleo(s) tide analogs cannot target and destroy the cccDNA.Nowadays, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing technology can specifically target the HBV cccDNA, which brings hope to conquer HBV.Researches of CRISPR/Cas9 technology applied to clear HBV have been reviewed in this paper.

More recently, a high incidence of antisperm antibodies in men and women with unexplained in fertility has been reported by many authors. However, the antisperm antibodies may play a role in mediating in fertility by interacting with sperm. The effect of antisperm antibodies on the phagocytosis of rat peritoneal macrophages were studied in vitro. The results indicated that opsonization of rat sperm by rabbit anti-rat sperm antibodies increases both the percentage of phagocytosis and phagocytic index apparently. The engulfed sperm when observed by SEM were bended and wrapped up. It seems likely that antisperm antibodies enhanced phagocytosis by macrophages may take part in the antisperm immune responses.

We studied the effects of oxymatine,an alkaloid from Sophra Flavesens Ait,on human andmouse's lymphocytes' cytoplasmic free calcium,calium uptaking,cAMP & cGMP level.Theresults show that:(1).Oxymatrine can raise the cytoplasmic free calcium.(2).It can also de-crease the cAMP level of PHA preactivated human tonsillar lymphocytes (3).It has no effect oncGMP level of lymphocyte.(4).It has no effect on the calcium uptaking.Combined with ourprevious report,we think that oxymatrine may influence cells' [Ca++] and cAMP level via af-fecting cell membrane.

Objective To explore the outcome and diagnostic methods of umbilical cord winds of the perinatal. Methods Retrospective analysis of 2350 cases of umbilical cord winds in our hospital was conducted. We selected 2400 cases as control. We calculated both the incidence rate of cesarean section, fetal distress and neonatal hypoxic - ischemic encephalopathy. Results Umbilical cord winds accounted for 20.1 percent of the total number of deliveries in the same period. The incidence rate of fetal distress and cesarean section was significantly high. Bsound ultra was 98.9% diagnosis. The electronic fetal care was 99.7% in diagnosis. Color Doppler ultrasound was 100% in diagnosis. Conclusion Umbilical cord winds is a common complication of pregnancy, and decision is also an important factor for delivery mode. Umbilical cord winds will increase obviously in the incidence of fetal distress and perinatal morbidity. The main method for prenatal diagnosis is the color Doppler ultrasound examination, such as B - and E - fetal care. Color Doppler ultrasound technique is the best way for non - invasive is observation of intrauterine blood circulation changes. In helpful for the judge of the progress and outcome of pregnancy, fetal growth and development of the situation.

Anopheles minimus was infected with Plasmodium yoelii yoelii under adequate condition. The malaria parasite could develop normally and produce mature oocysts containing sporozoites. Large numbers of free active sporozoites were found in the haemocele but most of them failed to penetrate into the mosquito's salivary glands. Thus the sporogonic cycle of the parasite could not be completed.But in one mosquito, a few sporozoites were found in its salivary glands. It seems that there is an individual difference in the susceptibility of the mosquitoes and this throws some light on the possibility of selecting a susceptible strain of A. minimus to establish the A. minimus-P. yoelii system for malaria research.

Objective Immunoreactive intensity of dopamine transporter(DAT)was quantitatively analyzed in the different regions of postmortem human brain in order to provide evidence in selecting an appropriate reference region for neuroimaging in measurement of the altered DAT.Methods The brain tissue blocks taken from 8 male cadavers were paraffin-embedded and sectioned,and immunoautoradiography was used to display the difference of DAT immunoreactive intensity in the substantia nigra,putamen and caudate nucleus,cingulate cortex,frontal cortex,occipital cortex and cerebellar cortex.Results The highest DAT density was found mainly in the substantia nigra,caudate nucleus and putamen,while the lowest density was only seen in cerebellar cortex.Quantitative analysis revealed that the intensity of DAT immunoreactivity(DAT-IR)in cerebellar cortex was respectively 1/3.50,1/3.72,1/1.28 of that in frontal cortex,cingulate cortex and occipital cortex,and it was only 1/8.33,1/11.67,1/8.56 that of substantia nigra,caudate nucleus and putamen.Conclusions The cerebellar cortex has the lowest DAT as compared to other examined brain regions,and it can be used for a reference region in neuroimaging to detect altered DAT.

Objective: To investigate the therapeutic effect of acupuncture plus herbal medicine on vascular dementia (VD). Methods: Thirty cases of vascular dementia were treated by acupuncture plus Chinese herbal medicine for 2 months, in comparison with western medication (Nimodipine) in the control group, to observe the evaluation value of Hasegawa Dementia Scale (HDS) before and after the treatments between the two groups, the clinical therapeutic effect and changes of plasma atrial natriuretic peptide (ANP) before and after the treatments between the two groups. Results and Conclusion: There were significant differences in intelligence and therapeutic effect between the two groups (P＜0.01) before and after the treatment, but the therapeutic effect was better in the acupuncture plus medicine group than in the western medication group. Judging from content of atrial natriuretic peptide, there was no significant difference between VD patients and normal adults before the treatment (P＞0.05). Plasma ANP significantly increased in the acupuncture plus medicine group and western medicine group after the treatment (P＜0.01), more remarkably in the acupuncture plus medicine group (P＜0.01).

Hereditary nonpolyposis colorectal cancer as a eolorectal cancer is of most hereditary characters.With developing of the molecular genetics on detection technology,mismatch repair gene has already been detected by multi-approach,and been applied to screen hereditary nonpolyposis eolorectal cancer patients.In this artical,we mainly discuss the correlated study progression between hereditary nonpolyposis eolorectal cancer and several mismatch repair genes such as MSH2,MLH1,MSH6,PMS1,PMS2,MLH3 and EXO1.