Objective To investigate the protective effects of hydrogen-rich water postconditioning on glutamate (Glu) mediated ischemia/reperfusion (I/R) injury in isolated brain slices of neonatal rats and explore its mechanism of action.Methods The brains of Sprague-Dawley (SD) neonatal rats aged 7 days were cut into slices and cultured. And then the cultured slices were randomly divided into the normal control group, Glu injury group (1 mmol/L Glu for 30-minute injury), hydrogen-rich water postconditioning group (after Glu injury for 30 minutes, cultured with complete medium containing 100μmol/L of hydrogen-rich water), once per 3 hours to change the medium for totally 24 hours, each group having 12 holes. The brain slices were stained by hematoxylin-eosin (HE) staining to observe the changes of nerve cells. The lactate dehydrogenase (LDH) release rates, the numbers of nissl bodies and the basic fibroblast growth factor (bFGF) in each brain slice were determined to evaluate the degree of cerebral neuronal damage.Results Compared with the normal control group, the number of nerve cells was rare, and the structure not complete; the LDH release rate and the number of bFGF were increased significantly in Glu injury groups [LDH release rates: (50.66±4.93)% vs. (20.15±5.14)%, bFGF (cells/400 power field): 22.79±2.13 vs. 4.13±1.17, both P < 0.01); the Nissl body was decreased (cells/400 power field: 11.81±2.69 vs. 47.10±3.78,P < 0.01) in Glu injury group. Compared with Glu injury group, the morphological structure of brain nerve cells was restored, the LDH release rate was reduced [(39.13±3.66)% vs. (50.66±4.93)%]; bFGF was decreased (cells/400 power field: 14.22±1.22 vs. 22.79±2.13), and the Nissl body was increased (cells/400 power field: 23.25±6.05 vs. 11.81±2.69) in hydrogen-rich water postconditioning group (allP < 0.05).Conclusions Hydrogen-rich water postconditioning has protective effects on rat brain slices with I/R injury induced by Glu. Its mechanism was possibly related to the reduction of free radicals, calcium overload and inflammatory factors induced by excitatory amino acids toxicity, resulting in inhibition of cell apoptosis.

Objective To clarify the features of malignant ovarian neoplasms arising from ovarian endometriosis Methods A total of 26 women with malignant ovarian neoplasms arising from ovarian endometriosis were analyzed retrospectively Results Dysmenorrhea and pelvic mass were the most common clinical features Among 18 cases who underwent B ultrasound or color doppler ultrasound examination, solid foci in the pelvic masses were found in 10 cases The main histologic types of ovarian malignancy were endometrioid adenocarcinoma and clear cell carcinoma Microscopically atypical endometriosis with the tumors were found in 15 cases International Federation of Gynecology and Obstetrics stage:stage Ⅰ 21(81%)cases,stage Ⅱ 3(12%)cases,stage Ⅲ 2 (8%)cases Conclusions Clinical diagnosis of malignant ovarian neoplasms arising from ovarian endometriosis in early stage is difficult, and B ultrasound examination is more valuable for diagnosis It is suggested that close serutiny of endometrial hyperplasia, cellular atypia and malignancy in ovarian endometriosis be essential to understand the origin and development of malignant neoplasms arising from ovarian endometriosis

Objective To study the changes of neuron-specific-enolase (NSE), S100B protein and neuropeptide Y (NPY) levels in serum and cerebrospinal fluid of children with viral encephalitis and their clinical significance. Methods The NSE, S100B protein and NPY levels in the serum and cerebrospinal fluid of 50 children with viral encephalitiswere were measured, and another 20 children without central nervous system infection were selected as controls. Results The NSE, S100B protein and NPY levels in the serum and cerebrospinal fluid of children with viral encephalitis[serum: (18.90 ± 5. 50)μg/L, (0. 57 ±0. 26) μg/L, (267. 3 ± 54. 7 ) μg/L; GSF: ( 10. 45 ± 4. 40) μg/L, (0. 93 ± 0. 53 ) μg/L, (347.2 ± 60. 6) μg/L] were higher than those in control group [serum: ( 10. 35 ± 2. 49 ) μg/L, ( 10 ± 0. 06 ) μg/L, ( 67. 8 ±22.5)μg/L;GSF:(3.96 ± 1.57)μg/L,(0. 29 ±0. 18)μg/L,(102.6 ±38.9) μg/L] ( P <0.01). The levels of serum and CSF NSE S100B protein and NPY in critical patient[serum: (21.93 ±5.39)μg/L,(0.71 ±0. 31)μg/L, (32. 5 ± 62. 8) μg/L;GSF: (13.05 ±4.41)μg/L, (1.23 ± 0. 66) μg/L, (407.3 ±68. 1 ) μg/L] were higher than ordinary patients [serum: ( 15.93 ± 4. 02 ) μg/L, ( 0. 42 ± 0. 14 ) μg/L,(234.7 ±51.2)μ.g/L;GSF:(8.05 ± 1.77) μg/L,(0. 63 ±0.26)μg/L, (320.2 ±59.5) μg/L] ( P <0. 01 ). Conclusion NSE, S100B protein and NPY can be used to evaluate encephalitis condition, brain damage degree and prognosis of viral encephalitis.

Objective To study the bioactive components from stems of Schisandra propinqua var. intermedia. Methods Compounds were separated with acombination of multi-chromatography. Their che- mical structures were determined on the basis of spectral analysis and chemical evidence. Results Nine compounds were isolated from S. propinqua var. intermedia. The structures were elucidated as vanillic acid- 4- O-?-D-allopyranoside (Ⅰ), salidroside (Ⅱ), 2-hydroxy-5-(2-hydroxyethyl)-phenyl-?-D-glucopyranoside (Ⅲ), 3, 4-dimethoxyphenyl-4-?-D-glucopyranoside (Ⅳ), 3, 5-dihydroxybenzoic acid-4-O-?-D-glucopyranoside (Ⅴ), 3, 5-dimethoxy-4-hydroxy-benzoic acid (Ⅵ), 4-methoxy-benzoic acid (Ⅶ), vanillic acid (Ⅷ) and protocatechuic acid (Ⅸ). Conclusion Compound Ⅰ is a new phenolic alloside and others are isolated from S. propinqua var. intermedia for the first time.

Objective To determine the changes in serum and urine vitamin D binding protein ( VDBP) concentrations in type 2 diabetes, and to explore the clinical significance. Methods The serum and urine VDBP concentrations in 102 healthy individuals and 106 type 2 diabetic patients were determined by ELISA. For analysis and comparison, 106 type 2 diabetic patients were divided into imperfect glycemic control subgroup and perfect glycemic control subgroup, microalbuminuria subgroup and normal albuminuria subgroup. Results The cut-off point of serum VDBP concentrations was 60. 6 μg/ ml and the cut-off point of the urine ratio of VDBP and creatinine was 7. 76 mg/ g, and both were determined according to the upper limit of 97. 5 % credit intervals in 110 healthy individuals. Serum VDBP concentration and the urine ratio of VDBP to creatinine in type 2 diabetic patients were significantly higher than those in the healthy individuals ( P < 0. 01 ), the imperfect glycemic control subgroup had higher serum VDBP concentrations and the urine ratio of VDBP to creatinine than those in the perfect glycemic control subgroup ( P <0. 05). The microalbuminuria subgroup had higher urine ratio of VDBP to creatinine than that in the normal albuminuria subgroup ( P<0. 01). Urine ratio of VDBP to creatinine in diagnosing early diabetic nephropathy had sensitivity of 96. 4 % , specificity of 68 % , and concordance of 83% . Conclusion Detection of serum VDBP levels has some reference value in understanding the state of diabetes. Combined determinations of urine ratio of VDBP to creatinine and ratio of albumin to creatinine have significant clinical value in the early diagnosis of diabetic nephropathy.

Objective To evaluate the valRe of Geneva score,plasma D-dimer lUmitel,lower extremity compressive ultrasonography and transthoracic echocardiography,as well a8 their combination,in diagnosis for suspected pulmonary thmmboernbolism(PTE)and its exclusion.Methods In total,139 confirmed FrrE patients were enrolled in the study,with 50 patients with suspected PTE at admission but excluding PTE after testing as controls,Geneva scores and plasma level of D-dimer were determined,and deep vein uhrasonography in the lower extremity and transthoracic echocardiography were performed for all the confirmed cases of PTE and controls.Diagnostic values were evaluated with each teat index alone or in combination,to confirm or exclude PTE.Results FrrE could be diagnosed by hish Geneva score,with a positive likelihoed ratio more than 10 and it could not be excluded by a negative likelihood ratio more than 0.1 with Latex semi.quantitative method and quantitative methed Latex method P,rE could be excluded by a multi-tests in parallel with negative likelihoed ratio less than 0.1.High Geneva scores,in combination with ultrasonography of the lower extremity and transthoracic echoeardiography in combination with Youden index greater than 0.6 could indicate PTE.Sensitivity and specificity of P1'E diagnosis could be improved by multi-tests in parallel or in series.Conclusions Geneva SCOre is more objective indicator and hish score has diagnostic value for PTE.PTE could be excluded reliably by negative result of multi-diagnostic tests in paralleL Misdiagnosis and under-diagnosis for PTE can be reduced by Geneva score,blood D-dimer level,lower extremity compressive ultrasonogaphy and transthoracic echocardiography,as well as their combination,in parallel in hospitals without ECT or spiral CT.

Objective: The purpose of this study is to understand associations of developmental coordination disorders (DCD) and sensory integration disorders (SID) with family environment for motor development. Methods: A questionnaire survey was conducted among 309 parents of 3-4 years old children in two kindergartens in Beijing selected by clustering sampling, using Development Coordination Disorder Questionnaire, Sensory Integrative Function Scale and Family Environment Scale on Motor Development for Urban Preschool Children. Results: Among the 309 children, the prevalence of DCD was 8.1% with gender difference with boys(11.0%) significantly higher than girls(4.4%)(χ2=6.71，P<0.05). The rate of SID was 42.1% with no significant gender difference(χ2=7.32，P=0.62). The concordance rate of SID and DCD(23/25，92.0%) was related to family environment for motor development. Specifically, parental rearing patterns was the primary factor for co-occurrence of SID and DCD(B=-0.37, OR=0.69, 95%CI=0.54-0.89，P<0.01). Conclusion The development of SID and DCD is closely related among preschoolers. Parental rearing pattern plays an important role in SID and DCD.

Object To investigate the chemical constituents of Erigeron breviscapus (Vant.) Hand. Mazz. (Compositae) Methods Compounds were isolated from the ethanolic extract with column chromatography and identified on the basis of spectral analysis (IR, EI MS, FAB MS, 1HNMR, 13 CNMR, 2DNMR) and physiochemical properties Results Two compounds were identified as 1 hydroxy 2,3,5 trimethoxy xanthone (Ⅴ), and 1 (2′ ? pyranone) 6 caffeoyl ? D pyranoglucose (Ⅵ) Conclusion Both of the two compounds were new Compound Ⅵ showed inhibitory effects on the adhesion of endothelial induced by TNF ?, indicating its protective effect against injury due to ischemic reperfusion

Objective: To investigate the chemical constituents of Erigeron breviscapus (Vant.) Hand. Mazz. Methods: The CHCl 3 extract was isolated and purified with the silica gel column chromatography. The compounds were determined on the basis of spectral analysis(IR, MS, 1HNMR and 13 CNMR). Results: Six compounds were isolated and the structures were identified as 3, 4 dihydroxy phenyl acrylic acid (Ⅶ), ? methoxy ? pyranone (Ⅷ), stigmasterol (Ⅸ), stigmasterol 3 O ? D glucopyranoside(Ⅹ), ? sistosterol (Ⅺ), ? sistosterol 3 O ? D glucopyranoside (ⅩⅡ). Conclusion: Compounds Ⅶ,Ⅷ,Ⅺ,ⅩⅡ are isolated from this plant for the first time. [

AIM　To isolate and identify the active constituents of Erigeron brevisapus (Vant.) Hand. -Mazz. METHODS　The chemical constituents were isolated by silica gel column chromatography and two new compounds were obtained. Their structures were elucidated by IR, MS, 1HNMR, 13CNMR, DEPT and 2DNMR. The injury of BCMEC (bovine cerebral microvascular endothelial cell) was determined by lactic dehydrogenase (LDH), the ability of the drugs anti-oxidation and scavenging oxidation of free radical was measured by colorimetric method. RESULTS　Two new compounds have been identified as 1-O-methyl-3, 5-O-dicaffeoyl quinic acid methyl ester (III) and 5-O-caffeoyl quinic acid butyl ester (IV). CONCLUSION Compounds III and IV are new compounds. Compound III can protect BCMEC injury by LPC.