Gene regulatory networks(GRN),which focuses on the complex interactions of genes in life,is an important part in the study of the functional genomics and is the frontier of bioinformatics research.Application of gene-chip technique in bioinformatics provides a great number of basic data for the research of GRN.This paper reviews the origin and recent development of GRN,explicates the preconditions and rationales for construction of GRN,and analyzes several classic GRN models: Boolean networks,linear models,non-linear models and Bayesian networks.The rationales,basic algorithms,advantages,disadvantages and applicability of the models are reviewed based on the characteristics of gene-chip data.

The particularity of physician - patient relationship determines a bilateral ethical regulation is called for in building up the harmony between doctors and patients.Discussion on this matter not only reminds us of pondering on the duality of interpersonal relationship in particular fields,but also demonstrates the necessity and urgency for relevant research on current bilateral ethical regulation.

Objective To investigate the protective effects of hydrogen-rich water postconditioning on glutamate (Glu) mediated ischemia/reperfusion (I/R) injury in isolated brain slices of neonatal rats and explore its mechanism of action.Methods The brains of Sprague-Dawley (SD) neonatal rats aged 7 days were cut into slices and cultured. And then the cultured slices were randomly divided into the normal control group, Glu injury group (1 mmol/L Glu for 30-minute injury), hydrogen-rich water postconditioning group (after Glu injury for 30 minutes, cultured with complete medium containing 100μmol/L of hydrogen-rich water), once per 3 hours to change the medium for totally 24 hours, each group having 12 holes. The brain slices were stained by hematoxylin-eosin (HE) staining to observe the changes of nerve cells. The lactate dehydrogenase (LDH) release rates, the numbers of nissl bodies and the basic fibroblast growth factor (bFGF) in each brain slice were determined to evaluate the degree of cerebral neuronal damage.Results Compared with the normal control group, the number of nerve cells was rare, and the structure not complete; the LDH release rate and the number of bFGF were increased significantly in Glu injury groups [LDH release rates: (50.66±4.93)% vs. (20.15±5.14)%, bFGF (cells/400 power field): 22.79±2.13 vs. 4.13±1.17, both P < 0.01); the Nissl body was decreased (cells/400 power field: 11.81±2.69 vs. 47.10±3.78,P < 0.01) in Glu injury group. Compared with Glu injury group, the morphological structure of brain nerve cells was restored, the LDH release rate was reduced [(39.13±3.66)% vs. (50.66±4.93)%]; bFGF was decreased (cells/400 power field: 14.22±1.22 vs. 22.79±2.13), and the Nissl body was increased (cells/400 power field: 23.25±6.05 vs. 11.81±2.69) in hydrogen-rich water postconditioning group (allP < 0.05).Conclusions Hydrogen-rich water postconditioning has protective effects on rat brain slices with I/R injury induced by Glu. Its mechanism was possibly related to the reduction of free radicals, calcium overload and inflammatory factors induced by excitatory amino acids toxicity, resulting in inhibition of cell apoptosis.

Positron emission tomography(PET) plays an important role in the research of substance distribution in Human body. The distribution of substance in whole body is carried out by the transport via circulation system and exchange between blood and tissue which occurs in microvascular. Both the research of PET and of microvascular are focused on the transport and exchange of substance in human body. So it should be meaningful to apply PET image to the research of microvascular, and to use the microvascular theory to improve on the analysis of PET image. In this article, some research in this domain are introduced, and foreground is reviewed.

Displacement ventilation is described from the aspects of its primary principle, characteristics and differences from the traditional one. The factors that have to be taken account into when a displacement ventilation system designed are also discussed.

Objective To reform the traditional teaching methods,and to explore the feasibility and effect of micro-seminar applied in Pediatric Nursing teaching.Methods A total of 151 undergraduate students of nursing admitted in the year of 2011 were selected as the experimental group,using micro-Seminar teaching method,while 152 undergraduate students of nursing admitted in the year of 2010 as historical control (the traditional teaching group).The application effect of micro-Seminar in Pediatric Nursing teaching was investigated and evaluated.Results Students of micro-seminar group got higher score in the final examination than students of the traditional teaching group [(81.34 ± 8.34) vs.(78.40 ± 8.03)],and the difference was statistically significant (P＜0.05).Micro-seminar was accepted by 92.7％ of students of the experimental group as a new teaching method,and students unanimously affirmed it was beneficial to enhancing their individual abilities of learning,cooperation,communication,and expression.Conclusions Micro-seminar pedagogy could help cultivating students' comprehensive abilities and improving teaching effect and quality.As was high operability,micro-Seminar was worth applying and popularizing.

ObjectiveTo evaluate the therapeutic efficacy of acupuncture plus fumigating eyes with cool Chinese medicinal fog in treating severe dry eye syndrome (DES).MethodTwenty-six outpatients with moderate-severe DES patients were classified into 11 cases of heat damaging yin type (20 eyes) and 15 cases of phlegm-stagnation type (23 eyes), to simultaneously receive acupuncture and fumigating eyes with cool Chinese medicinal fog. Tear break-up time (BUT), tear production (SchirmerⅠtest, SIT), and subjective symptoms score were observed and analyzed before treatment, 1 month, 3 months, and 6 months after treatment.ResultThe SIT, BUT, and subjective symptoms score 1 month, 3 months, and 6 months after treatment were superior to that before treatment (P<0.01); The SIT, BUT, and subjective symptoms score 6 months after treatment were lower than that of 3 months after treatment (P<0.05) but still significantly different from that before treatment (P<0.05).ConclusionAcupuncture plus fumigating eyes with cool Chinese medicinal fog can improve the symptoms and signs of DES, though the therapeutic efficacy decreases during the long-term follow-up study.

The subject service platform was constructed for nursing science using Drupal according to the low edu-cation level, high work load, much fragmentary time, and low information literacy of nursing staff.Web 2.0 infor-mation service was provided for nursing staff,such as literature pushing, cloud tag revealing and personal customiza-tion by integrating the library-holding resources ( including paper literature resources , electronic literature re-sources and literature resources on networks ) .The literature literacy level and scientific research ability of nursing staff were improved by making the full use of their fragmentary time.The new service mode in hospital libraries was studied in Web 2.0 environment .

Objective To evaluate the air quality in the long-distance bus with air conditioner and the uncomfortable index of the passengers during the trip. Methods The air quality in the long-distance buses with air conditioner which were running for 3 hours,5 hours,7 hours respectively was monitored and 200 passengers were investigated by questionnaire during the trip,Jan 2004 -Aug 2005. Results As the running time increasing,CO2 concentration significantly increased. The bacterium,fungus,PM10 and wind speed were increasing gradually,but still in the normal level. The temperature,CO,formaldehyde,benzene,toluene and dimethyl benzene did not increase. The hemolysis streptococcus was not found,the relative humidity droped a little but in normal level. The investigation results of questionnaire showed that 21.5%(43/200) passengers felt peculiar smell,64.5% (129/200)of them appeared lassitude symptom,43% (86/200)of them appeared the symptom of dizzy,thirsty and sore throat,21%(42/200)of them felt eye dry,14.5%(29/200)of them appeared the symptom of headache and breath-block,7%(14/200)of them felt nauseated and skin itch during the trip. Conclusion The CO2 pollution in the investigated long-distance buses is serious and this can impact the health of the passengers.

OBJECTIVE:To observe clinical efficacy of acitretin combined with TGP in the treatment of psoriasis vulgaris. METHODS:81 patients were randomized into control group(n=40)and treatment group(n=41). Control group were treated with 0.5 mg?kg-1?d-1acitretin then the dose of acitretin was adjusted to 0.1～0.2 mg?kg-1?d-1after clinical symptom improved.Treat-treatment course. Blood and urine routine,liver and kidney function and the level of blood lipid of 2 groups were detected before and after 8 weeks treatment as well as ADR. RESULTS:The total response rate was 90.2% for treatment group and 62.5% for control group,there were significant difference(P