Objective To conduct a retrospective analysis of the relationship between the time of rehabilitation intervention and its effectiveness among hemiplegic patients after cerebral infarction.Methods Fifty-four hemiplegic stroke patients within 1 week of onset were randomly divided into three equal groups of 18:In the A group rehabilitation was begun 1 to 2 weeks after onset; in the B group it was started between 2 and 4 weeks; in the C group rehabilitation was begun after four weeks.The patients all received comprehensive hemiplegic limb training,electric standing bed training,functional electrical stimulation and medical gymnastics for hemiplegia.Motor function was assessed using the Fugl-Meyer balance function assessment and the modified Barthel index before treatment,in the course of the first week of treatment and 3 months later.Results A group and B group made similar progress,but the curative effect in group C was significantly weaker.Conclusion Rehabilitation should be started within 1 month after the onset of cerebral infarction.

Objective To observe the influence of telephone follow-up and home follow-up on compliance behavior and activities of daily living (ADL) of stroke patients during rehabilitation. Methods 50 cases were as telephone follow-up group and 50 cases were as home follow-up group extracted from 355 stroke patients who discharged the hospital during August 1, 2010 to July 1, 2012. They were followed up 2 weeks, 1 month and 3 months after discharge respectively. Their ADL were assessed with Bathel index (BI) at discharge and 3 months after discharge, and the compliance behavior were assessed 3 months after discharge. Results There was no significant difference in the score of BI between 2 groups at discharge (P>0.05), and the score of BI improved in both groups 3 months after discharge (P<0.05), but it was higher in the home follow-up group than in the telephone follow-up group (P<0.05), as well as the rate of compliance behavior (P<0.01). Conclusion Regular home follow-up can improve the compliance behavior of stroke patients during rehabilitation.

Objective To investigate the abnormal brain activity of first-episode depression by rest-ing state functional magnetic resonance imaging ( fMRI) .Methods Twenty-one adolescent participants diag-nosed with depression(AD) and 18 healthy controls ( HC) were recruited.Resting state fMRI brain scans were performed on all participants.Regional homogeneity ( ReHo) approach was applied to preprocess the fMRI datasets.The value of ReHo maps were obtained in the whole brain.Results ReHo values in the AD group were higher than those in the healthy controls in the right inferior temporal gyrus ( MINI:66,-24,-20) ,left upper cingulate cortex (-27,47,-6) ,frontal polar (-24,59,14) ,after upper left cingulate cortex (-1,-16,35),after the bottom left cingulate cortex (-2,-38,32),left praecuneus (-1,-48,65) com-pared with that in the healthy controls (P<0.05) .ReHo in the AD group decreased in the right middle tempo-ral gyrus (45,-34,32) compared with that in the healthy controls (P<0.05).Conclusion Extensive ReHo abnormalities were found in the brains of patients with first-episode,drug-naive depression,and these abnor-malities in spontaneous neural activity may contribute to the neuropathology of adolescent depression.

Objectives: By observing and evaluating the clinical curative effect systematically of electrical acupuncture (EA) on Tianshu (ST 25) on diarrhea-predominant irritable bowel syndrome (D-IBS), to make it benefit for the application and spread of EA on Tianshu (ST 25)on D-IBS. Methods: 103 D-IBS matched the involved standards were allocated into treatment group (EA on ST 25, n=53) and control group (EA on Daheng, SP 15, n=50) in randomized,controlled and single-blind ways. Curative effect differences were analyzed by Ridit analysis.Results: The incidence of diarrhea, abdominal pain, abdominal distention or abdominal discomfort, borborygmus, defecation urgency and defecation incompletion feeling were 100%,89.3%, 71.8%, 74.8%, 83.5% and 78.6% respectively in D-IBS. Generally, after treatment, 11cases got excellent results, 34 cases improved and 8 failed in treatment group, totally effective rate was 84.9%; while in control group, 4 cases got excellent effects, 26 improved and 20 failed,totally the effective rate was 60.0%. Conclusion: Compared with control group, EA on Tianshu (ST 25) has a better curative effect on D-IBS.

OBJECTIVETo detect the role of surviving (SVV) in the protective effect of resveratrol against hypoxia/reperfusion injury (H/RI) of cardiac microvascular endothelial cells (CMECs).

METHODSCMECs isolated from the hearts of adult rats were exposed to hypoxia (94% N₂, 5% CO₂, 1% O₂) for 2 h followed by 4 h reoxygenation (95% O₂, 5% CO₂). The cell proliferation of CMECs was measured by MTT assay and Transwell method was used to detect migration ability of CMEC, PI-AnnexinV double staining and flow cytometry technique were employed to observe the apoptotic rate of CMECs. The SVV protein expression was detected with Western blot method.

RESULTSCompared to control group, the proliferation (0.19 ± 0.03 vs. 0.42 ± 0.07, P < 0.01) and migration ((28 ± 2)/5HPF vs. (50 ± 3)/5 HPF, P < 0.01) abilities were impaired and the apoptosis index ((19.7 ± 0.8)% vs. (5.4 ± 0.3)%, (P < 0.05) of CMEC was increased after H/RI. The proliferation (0.36 ± 0.07 vs. 0.19 ± 0.03, P < 0.05) and migration ((55 ± 3)/5HPF vs. (28 ± 2)/5HPF, P < 0.05) abilities of CMEC were significantly improved while the apoptosis index ((9.6 ± 0.7)% vs. (19.7 ± 0.8)%, P < 0.05) was significantly decreased in H/RI+resveratrol group compared to H/RI group.SVV protein expression was also upregulated in H/RI+resveratrol group compared to H/RI group (P < 0.05). To further ascertain the role of SVV in the protective effects of resveratrol, PI3K specific inhibitor LY294002 was added to H/RI+resveratrol group, the proliferation (0.25 ± 0.05 vs. 0.36 ± 0.07, P < 0.05) and migration ((34 ± 3)/5HPF vs. (55 ± 3)/5HPF, P < 0.05) abilities were significantly decreased, the apoptosis index ((16.2 ± 0.6)% vs. (9.6 ± 0.7)%, P < 0.05) was increased and the protein expression of SVV was downregulated (P < 0.05) in LY294002+H/RI+resveratrol group compared to H/RI+resveratrol group.

CONCLUSIONResveratrol could significantly reduce H/RI induced apoptosis and attenuate H/RI induced cardiac microvascular endothelial cells dysfunction through up-regulating PI3K/Akt/SVV pathways.

Animals ; Apoptosis ; Cell Proliferation ; Chromones ; Endothelial Cells ; Enzyme Inhibitors ; pharmacology ; Heart ; Hypoxia ; Morpholines ; Myocardium ; Myocytes, Cardiac ; Phosphatidylinositol 3-Kinases ; drug effects ; metabolism ; Proto-Oncogene Proteins c-akt ; drug effects ; metabolism ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury ; drug therapy ; Stilbenes ; pharmacology ; Up-Regulation