Objective To conduct a retrospective analysis of the relationship between the time of rehabilitation intervention and its effectiveness among hemiplegic patients after cerebral infarction.Methods Fifty-four hemiplegic stroke patients within 1 week of onset were randomly divided into three equal groups of 18:In the A group rehabilitation was begun 1 to 2 weeks after onset; in the B group it was started between 2 and 4 weeks; in the C group rehabilitation was begun after four weeks.The patients all received comprehensive hemiplegic limb training,electric standing bed training,functional electrical stimulation and medical gymnastics for hemiplegia.Motor function was assessed using the Fugl-Meyer balance function assessment and the modified Barthel index before treatment,in the course of the first week of treatment and 3 months later.Results A group and B group made similar progress,but the curative effect in group C was significantly weaker.Conclusion Rehabilitation should be started within 1 month after the onset of cerebral infarction.

We investigated the distribution of Chlamydia trachomatis genotypes in the outpatients visiting STD clinics in Fuzhou City ,China .Between 2013 and 2014 ,a total of 2 019 urethral or endocervical specimens from susceptible genital C .trachomatis infections were collected and detected by using real‐time PCR assay kit .The C .trachomatis‐positive specimens were subsequently amplified with nested PCR for Omp1 region and analyzed by sequencing .Genotyping was performed by BLAST similarity search and phylogenetic tree analysis .Eighty‐three of the 86 C . trachomatis‐positive specimens were suc‐cessfully sequenced .Only one specimen was identified as multiple infection .Eight different genotypes were identified .The most prevalent was F (36 .59% ) ,followed by E (24 .39% ) ,D (12 .20% ) ,G (9 .76% ) ,J (9 .76% ) ,H (3 .66% ) ,K (2 .44% ) and B (1 .22% ) .Synonymous mutation rate and non‐synonymous mutation rate among Omp1 genes of different geno‐types were calculated .All genotypes but G showed dN/dS values of<1 .Genotypes F and E were commonest .Synonymous sub‐stitution was the major pattern of variation in the process of Omp 1 gene evolution .

OBJECTIVE:To analyze the relative factors of ADR in our hospital.METHODS:A total of 185 ADR case reports collected in 2006 were analyzed statistically.RESULTS:Of the total 185 cases,33(17.8%) were reported by physicians,12(6.5%) were new and severe ADR,62.7% were reports from pharmacy department,infusion room and department of derma-tology.For a few departments,the ADR case reports were zero.121(65.4%) were caused by anti-infective drugs;138(74.60%) were caused by intravenous drip and 108(58.4%) manifested as lesions of skin and its appendages.CONCLUSION:The incidence of ADR is related to multi-factors;however,rational use of drugs is the key conducive to the reduction of ADR incidence.

Objective To observe the influence of telephone follow-up and home follow-up on compliance behavior and activities of daily living (ADL) of stroke patients during rehabilitation. Methods 50 cases were as telephone follow-up group and 50 cases were as home follow-up group extracted from 355 stroke patients who discharged the hospital during August 1, 2010 to July 1, 2012. They were followed up 2 weeks, 1 month and 3 months after discharge respectively. Their ADL were assessed with Bathel index (BI) at discharge and 3 months after discharge, and the compliance behavior were assessed 3 months after discharge. Results There was no significant difference in the score of BI between 2 groups at discharge (P>0.05), and the score of BI improved in both groups 3 months after discharge (P<0.05), but it was higher in the home follow-up group than in the telephone follow-up group (P<0.05), as well as the rate of compliance behavior (P<0.01). Conclusion Regular home follow-up can improve the compliance behavior of stroke patients during rehabilitation.

Objective To investigate the effects of autoantibodies (β1-AA) against second extra-cellular loop of the β1-adrenergic receptor (β1-AR-ECⅡ) in sera of patients with dilated cardiomyopathy (DCM) on proliferation of rat CD4+T lymphocytes.Methods β1-AA in the sera of patients with DCM was purified by affinity chromatography .CD4+T lymphocytes were isolated by immunomagnetic microbeads from peripheral blood mononuclear cells of rats and its positive rate was detected by flow cytometry .CCK-8 meth-od was used to detect the proliferation of CD 4+T lymphocytes and flow cytometry was performed to measure the ratio of CD4+/CD8+T lymphocyte .Results The purity of isolated rat CD 4+T lymphocytes by immu-nomagnetic microbeads reached 97.7%.The proliferation of CD4+T lymphocytes stimulated by CD3/CD28 was inhibited by β1-AA in a concentration-dependent manner .However , IgG antibodies extracted from sera of healthy controls did not suppress lymphocyte proliferation (P>0.05).The suppression effect of β1-AA was inhibited after binding to antigenic peptides corresponding to β1-AR-ECⅡ and was completely blocked by metoprolol, a specific antagonist of β1-adrenergic receptor (β1-AR).In addition,β1-AA had no effects on the ratio of CD4+/CD8+T lymphocyte .Conclusion β1-AA isolated from DCM patients suppresses the pro-liferation of CD4+T lymphocytes through β1-AR pathway , which indicates that β1-AA can directly reduce the number of T lymphocytes and impair the function of T lymphocytes , resulting in immune system disorders and the development of DCM .

Objective To investigate the related factors of phlebitis caused by PICC and support effective preventive nursing measures. Methods 163 patients diagnosed as malignant tumor who first used peripherally inserted central catheters (PICC) were recruited. Patients were observed for 3 months. According to the diagnostic criterion of infection, 34 patients with phlebitis were divided into the phlebitis group and others were divided into the control group. All patients were investigated for general information survey :sex, age, tumor type, allergic history,location of inserted vein, one-time success rate,activity after catheter, elbow bending,phlebitis occurred time, grade and type. Laboratory markers:WBC,neutrophils,immune parameters, fasting plasma glucose. Trait anxiety inventory (T-AI)was used for assessment of emotional state. Case-control study between groups was carried out. Results The results of univariate analysis showed that location of inserted vein,times of inserting vein,activity of inserted limb,elbow bending,blood sugar level and emotion were associated with phlebitis. Sex, age, tumor type, allergic history, immune function,WBC,neutrophils were not involved with phlebitis. The results of multivariate logistic regression analysis showed that PICC through basilica vein was the protective factor. Multiple insertions, more activity of inserted limb,durative anxious emotion were the risk factors of phlebitis. Conclusions Different location of vein,times of inserting vein,activity of inserted limb,elbow bending,high blood glucose level and persistent anxious emotion are the risk factors of phlebitis. Taking effective preventive measures before,during, and after PICC can reduce the incidence of phlebitis.

Aim To isolate HIV-1 from the blood specimen of HIV-infected persons, and study on the features of the isolates. Methods PBMCs from 10 heparinized blood specimens of HIV-1 infected persons were collected to isolate the virus with PBMC cocultivation method. Neuramindase was added to the culture medium to raise the HIV isolation rate. The supernatant of cocultures were detected by p24 antigen capture assay and also IFA test was used to identify the isolates. Cell tropism of the HIV-1 isolates was tested byuse of H9 and MT4 cell lines, respectively. Results HIV-1 was isolated from9 of the 10 specimens, the isolation rate could reached 90%. The 9 isolates were used to infect MT4 cells, respectively, and all of them could cause cell syncytias.8 of the isolates showed only a transient infection in MT4 cells, but the isolate FJ113 could infect MT4 successively, OD values kept no changes even it passed 15 generations, therefore, it is consider as a rapid/high replicating virus. Conclusions Addition of neuramindase into T cell culture medium may useful to raise HIV isolation rate. Most of HIV-1 isolates in Fujian are M tropic.

Objective: To investigate the intelligence development of the low birth weight babies and related factors. Method: The development ability of 190 low birth weight babies was assessed with the Revised Gesell Development Schedule. The result was compared with that of normal control. Result: The development quotients (DQ) of low birth weight infants in motor, adaptive and personal/social areas at 1 and 2 years of age and in language at 2 years of age were significantly lower than that of the normal controls. The stepwise regression analysis showed that all performances in motor, adaptive, language and personal/social areas were significantly related to age, sex, head circumstance, low birth weight and feeding way of infants, and education of their parents. Conclusion: Low birth weight infants have poorer intelligence at the age of 1 and 2 years than normal controls.

Objective To evaluate the cilincial value of Min-probe sonngraphy(MPS)for submucosal protrusion lesion in gastric.Methods 82 cases with submucosal protrusion lesion in gastric were detected by Min-probe sonography(MPS)and treated according to the diagnosis.Results 82 cases were fibroid,38 stromal tumor,among which 12 cases from muscularis mucosa,26 case from the proper muscle layer,4 malignant stromal tumor,6 lipoma,3 angioma,3 gastric cysts,11 gastric polypi,8 heterotopic pancreas,9 pressures from outside organ.29 cases were accepted EMR and 18 cases accepted surgical operation.Conclusion MPS is more valuable in the diagnosis and the differential diagnosis of submucosal protrusion lesion in gastric and can guide the further therapy.

Objective The person's Mesangial Proliferative Glomemlonephritis (MsPGN) were divided into 3 types based on clini- cal manifestations: nephroticsyndrome, hematuria and proteinuria. To investigate the expression of CD11a, CD11b, CD62L and its signifi- cance in the kidneys patients with MsPGN. Methods CD11a, CD11b and CD62L expression in blood of MsPGN patients (n=35) were investigated by flow eytometry method, and the changes of these proteins in kidney were surveyed. Results In MsPGN, CD11a and CD11b expression in blood were significandy lower and CD62L expression in blood were markedly higher than that in normal humans. And the renal glomeruli of MsPGN also expressed CD11a, CD11b and CD62L. Conclusion The expression of CD11a, CD11b and CD62L are abnormal in MsPGN, and apoptosis may play certain role in the pathogenesis of MsPGN.