Objective To investigate the effects of Chaihu-Longgu-Muli Decoction (CLMD) on superoxide dismutase (SOD) and maiondiaidehyde (MDA) in patients with temporal lobe epilepsy (TLE).Methods 65 patients with TLE were randomly divided into Oxcarbazepine group (n =32) and Oxcarbazepine + CLMD group (n =33).After 28 d,the curative effect,SOD,MDA changes in serum and side effect were observed between two groups.Results The clinical effective rate in Oxcarbazepine + CLMD group was 87.8％,slightly higher than that of the Oxcarbazepine group(53.1％) (P ＜0.05).After treatment,serum SOD was significantly higher in the patients than before treatment in two groups,but serum MDA was significantly lower(P ＜ 0.05 or P ＜ 0.01),while the above changes were more obvious in the Oxcarbazepine + CLMD group (P ＜ 0.05).No statistically significant differences of side effect (P ＞ 0.05).Conclusions CLMD has higher curative effect with less side effect,which can lead to increase of the level of SOD and decrease of the level of MDA,and the mechanism is related to oxidative stress.

Objective:To evaluate the effects of different temperature management strategies on blood-brain barrier (BBB) and postoperative cognitive dysfunction (POCD) in the patients undergoing spinal fixation surgery.Methods:Ninety-six patients, aged 44-78 yr, weighing 45-104 kg, of American Society of Anesthesiology physical status Ⅰ-Ⅲ, undergoing spinal fixation surgery with combined intravenous-inhalational anesthesia, were divided into 3 groups ( n=32 each) by the simple randomization: warming blood transfusion and infusion group (group WBI), active warming group (group AW) and active warming plus selective brain cooling group (group SBC). In WBI group, the fluid for intraoperative intravenous infusion was warmed to 37 ℃ using a medical blood transfusion-infusion warmer.In AW group, warming was maintained using the fluid warming combined with the body surface warming blanket until the end of operation, and the target temperature of the warming blanket was set at 38 ℃.In SBC group, the warming protocol were similar to those previously described in AW group, and selective brain cooling was performed through an electronic ice cap with a set temperature of 4 ℃.Blood samples were collected from the median cubital vein at the end of operation, brain microvascular endothelial cells were isolated and identified by immunomagnetic bead, and the target cells were counted with a fluorescence microscope.The concentration of C-reactive protein in peripheral blood was measured by rate nephelometry on the first day after operation.The extubation time, length of post-anesthesia care unit (PACU) stay, total length of hospital stay, thermal comfortableness score, shivering in PACU, agitation and postoperative fever were recorded.The postoperative recovery was assessed using the 40-item Quality-of-Recovery scale on 7th day after surgery. Results:Compared with WBI group, the incidence of POCD and shivering in PACU, extubation time, length of PACU stay, and serum C-reactive protein concentrations were significantly decreased, and thermal comfortableness score and 40-item Quality-of Recovery scale score were increased in AW group and SBC group ( P<0.05). Compared with AW group, the count of brain microvascular endothelial cells was significantly decreased in SBC group ( P<0.05). Conclusions:Active warming produces no damage to the structure of BBB, and can reduce the development of POCD; active warming combined with selective brain cooling can improve the structural integrity of BBB, but it cannot reduce the development of POCD in the patients undergoing spinal fixation surgery.

Objective To select and evaluate the diluents for quantitative detection of HBV DNA of high loads sample,hope to find the most applicable diluents which could be used in clinical test.Methods The standard substance(2.00 × 109IU/ml)was 10 and 100 times diluted by different diluents,compare the result of test,and the bias was analysis taking negative quality control as standard diluents,negative serum,physiological saline,and distilled water as candidate diluents.Results When 10 times diluted,there was no statistically difference between the standard diluents and distilled water as diluents(t =2.04,P ＞ 0.05),the bias were less than the TEa regulated by professional standard.When used negative serum and physiological saline as diluents,the results were higher than that of standard diluents (P ＜ 0.05),and the ratio of the bias higher than the TEa was 16.67％ and 20.00％.When 100 times diluted,the results of candidate diluents were higher than that of standard diluents.In this time,the result of distilled water diluted detection presented a good linear relationship with the result of standard diluted detection,the formula was Y =0.963X + 0.267 (Y =result of standard diluted detection,X =result of distilled water diluted detection).All the bias were less than the TEa,the sequence of bias sort by ascending counts were negative quality control,distilled water,physiological saline and negative serum.Conclusions The most applicable diluents were negative quality control and distilled water with 10 times dilution.When 100 times diluted was used,the most applicable diluents was negative quality control,then was distilled water,physiological saline and negative serum.If using the distilled water to dilute,we could corrected the result by the formula Y =0.963X + 0.267 to ensure the result to be more exactly.

Objective This retrospective study is performed to analyze the epidemiological and clinical features of patients with infective endocarditis (IE) hospitalized in Fuwai Cardiovascular Hospital during the latest 7 years.Methods This retrospective study included a cohort of 368 infective endocarditis patients hospitalized in Fuwai Hospital form August 2005 to August 2012.Predisposing cardiac diseases,causative organisms,clinical features and outcomes were analyzed.Risk factors related to outcome including NYHA classes,causative organisms and complications,were evaluated.Results Among the IE patients,6.8％ (25/368) patients had rheumatic heart diseases 31.8％ (117/368) had congenital heart diseases,22.8％ (84/368) were post-PCI or operative endocarditis and IE developed in 14.1％ (52/368) patients without previous cardiac diseases.Blood culture positive rate was 46.2％ (170/368).Streptococci viridians [27.6％ (47/170)]were the most common causative organisms,followed by coagulase-negative staphylococci [15.9％ (27/170)].Fever and cardiac murmur were the most common clinical presentations.Congestive heart failure was the most common complication [87.8％ (323/368)].Systemic and pulmonary embolism occurred in 16.0％ patients,80.9％ IE was detected by echocardiography.In-hospital mortality rate was 6.7％,mostly due to refractory congestive heart failure and sepsis.Subgroup analysis showed that incidence of post-PCI or operative endocarditis was significantly higher in IE patients hospitalized after 2009 compared to IE patients hospitalized before 2009 (27.5％ vs.19.2％,P ＜ 0.05).Higher incidence of staphylococcal infection was evidenced in mechanical valves than in native valves(44.4％ vs.19.8％,P ＜ 0.05).Conclusion During the past decade,there is a significant change on epidemiology and clinical features of IE in China.Incidence of post-surgical and interventional IE increased significantly.Staphylococcal and Gram negative bacilli infection are major pathorganisms of endocarditis of mechanical valves.Due to the lower positive rate of blood culture,echocardiography serves as the most important diagnostic tool for infective endocarditis.

Objective:To investigate the rates of low disease activity and clinical remission in patients with systemic lupus erythematosus(SLE)in a real-world setting,and to analyze the related factors of low disease activity and clinical remission.Methods:One thousand patients with SLE were enrolled from 11 teaching hospitals.Demographic,clinical and laboratory data,as well as treatment regimes were collec-ted by self-completed questionnaire.The rates of low disease activity and remission were calculated based on the lupus low disease activity state(LLDAS)and definitions of remission in SLE(DORIS).Charac-teristics of patients with LLDAS and DORIS were analyzed.Multivariate Logistic regression analysis was used to evaluate the related factors of LLDAS and DORIS remission.Results:20.7％of patients met the criteria of LLDAS,while 10.4％of patients achieved remission defined by DORIS.Patients who met LLDAS or DORIS remission had significantly higher proportion of patients with high income and longer disease duration,compared with non-remission group.Moreover,the rates of anemia,creatinine eleva-tion,increased erythrocyte sedimentation rate(ESR)and hypoalbuminemia was significantly lower in the LLDAS or DORIS group than in the non-remission group.Patients who received hydroxychloroquine for more than 12 months or immunosuppressant therapy for no less than 6 months earned higher rates of LLDAS and DORIS remission.The results of Logistic regression analysis showed that increased ESR,positive anti-dsDNA antibodies,low level of complement(C3 and C4),proteinuria,low household in-come were negatively related with LLDAS and DORIS remission.However,hydroxychloroquine usage for longer than 12 months were positively related with LLDAS and DORIS remission.Conclusion:LLDAS and DORIS remission of SLE patients remain to be improved.Treatment-to-target strategy and standar-dized application of hydroxychloroquine and immunosuppressants in SLE are recommended.

In the original publication the grant number is incorrectly published. The correct grant number should be read as "17140901600". The corrected contents are provided in this correction article. This work was partially supported by grants from the National Natural Science Foundation of China (Nos. 81670470 and 81600149), a grant from the Shanghai Municipal Commission for Science and Technology (17140901600, 18411953500 and 15JC1400201) and a grant from National Key Research and Development Program (2016YFC0905100).

Adenosine ; genetics ; Adenosine Deaminase ; genetics ; metabolism ; Animals ; CRISPR-Associated Protein 9 ; genetics ; metabolism ; CRISPR-Cas Systems ; genetics ; Embryo, Mammalian ; metabolism ; Gene Editing ; Genetic Variation ; genetics ; Mice ; Mice, Inbred C57BL ; Rats ; Rats, Sprague-Dawley