Integrins are the main mediators of the complicated interaction between hepatic stellate cells (HSCs) and extracellular matrix (ECM) components. Signal transduction by integrins is initiated by both occupancy and crosslinking of integrins by ECM components. Focal adhesion kinase (FAK) and mitogen- activated protein kinase (MAPK), which are the downstream molecules, involve in integrin-mediated cellular behaviors. In this review, we briefly summarize the integrin's structure, intracellular signaling cascades and its role in HSC behaviors,such as activation,proliferation and apoptosis.

Objective To provide references foroptimizing the structure of human resourcesby investigating human resource development and the current situation of allocation in the hospital .Methods Descriptive statistical a-nalysis and Spearman rank correlation analysis were conducted on the data of human resource in the hospital from 2004 to 2013 and the situation of personnel allocation in the hospital at the end of 2013 was emphatically analyzed by the statistical description.Results The development of the total human resource from 2004 to 2013was adapted to the hospital development , but the development of staff at each positionwas at unbalance .The nursingstaff wasin short . The educational background structure of all kinds of positions was reasonable , but thestructure of professional titleswas unreasonable , without developing into talents team .Moreover ,the non-permanently-contracted staff took up a lar-ger proportion .Conclusion Hospitals need to achieve comprehensive development of human resource by setting up scientific conception of talents ,to improve the quantity and quality of nursing staff by enhancing nursing staff construc -tion, to forming an reasonable talent team by balancing the structure of personnel quality , and to stabilize the hospital staff structure by attaching importance to the management of non -permanently contracted staff .

Objective To investigate therapeutic effects of transurethral ureteroscopic pneumatic lithotripsy(TUPL).Methods A retrospective analysis was made on 320 cases of ureteral calculus treated with TUPL from September 2002 to August 2005.A Wolf F9.8 rigid ureteroscope and an EMS pneumatic ballistic lithotriptor were used.The probe was 0.8 mm or 1.0 mm in diameter.The pulse settings was 200~300 kPa.The fragmentation was conducted with single or multiple episode of treatment.Results The stone was pushed back to the renal pelvis in 6 cases and a conversion to extracorporeal shock wave lithotripsy(ESWL) was needed.The insertion of the ureteroscope was failed in 5 cases and a conversion to open surgery was required.The TUPL time was 2~10 min(mean,5.5 min) and the intraoperative blood loss was 3~15 ml(mean,7 ml).The stones were successfully fragmented on one session in 309 cases,the success rate being 96.6%(309/320).The length of postoperative hospital stay was 3~7 d(mean,4.5 d).There were 24 cases of mucosal injury of the ureter and 4 cases of false passage,all of which were cured with double-J catheter indwelling for 1 month.Follow-up examinations for 1~10 months(mean,4 months) showed stone clearance within 1 month and no ureteral stenosis or urinary infection.Conclusions Transurethral ureteroscopic pneumatic lithotripsy for the treatment of ureteral calculus is safe and effective.

Objective To observe the effects of nose-stuffy continuous positive airway pressure(CPAP)combined with large-dose Mucosolvan on the hyaline membrane disease of newborn(NHMD). Methods Third),divided into three equal parts,by intravenous drop infusion]. According to the results of SpO2 and blood gas analysis,the CPAP setting was adjusted. Before and after the treatment,the SpO2、RR、PaO2、PaCO2、SaO2 of all the children were analyzed. Results Ten children of all(76.9%) were cured,two died,one quitThe SpO2 of the total,at one hour after the treatment,improved significantly(P＜0.001);the RR、PaO2、SaO2 at 24 hours later also improved(P＜0.05). Conclusion The method of nose-stuffy continuous positive airway pressure(CPAP) combined with large-dose Mucosolvan,which had good effects on ameliorating the symptom and improving the gas exchange,is worth used in primary hospitals widely.

An animal model of acute lung injury was reproduced by intravenous injection of E. coli endotoxin to burned rats. The histological changes of lung were studied using transmission electron microscope. It was found that leukocytes accumulated in the pulmonary capillaries and adhered to endothelia. The WBC count decreased gradually. The level of MDA, lung coefficient and lung water content were increased. The SOD activity was decreased. Pretreatment with SOD could improve the changes mentioned above. This study suggests that oxygen radicals play an important role in acute lung injury induced by burns complicated by endotoxemia.

Objective:To investigate the effects of special immunotherapy in treatment vernal conjunctivitis. Methods:73 patients of vernal conjunctivitis with positive reaction of dermatophagoides farinae antigen were randomly divided into experimental group(36 patients was desensitizer treated with dermatophagoides farinae) and control group (37 patients with routine no special immunotheraty). Results:The total effective rate in experimental group was 91.6%,while in control group was 64.9% (P

AIM: To investigate the effect of IH764-3 on the apoptosis of H 2O 2-stimulated hepatic stellate cells(HSCs) and the alteration of focal adhesion kinase (FAK). METHODS: The proliferation of HSCs was examined by direct cell count and the apoptosis was determined by Annexin-V/PI labeling, while the morphological change was observed by light microscopy and transmission electron microscopy. In addition, FAK mRNA was detected by RT-PCR. RESULTS: H 2O 2 promoted the proliferation of HSCs. IH764-3 induced the apoptosis of HSCs in a dose-dependent manner. The HSC apoptotic rates of different groups were 6.35%,9.28%,15.10%,19.69%,respectively, after treated with different concentrations of IH764-3 for 48 h while H 2O 2 group showed 2.30%. In 30 mg/L group, the apoptosis rates were 6.73%?10.34%?15.10% for the indicated time periods(12 h, 24 h, 48 h). In the presence of IH764-3, FAK mRNA decreased. The FAK mRNA reduction began at 2 h after adding IH764-3. CONCLUSION: IH764-3 induced the apoptosis of HSCs. Down-regulating the expression of FAK mRNA may be one of its mechanisms.

STMN1 is a microtubule-destabilizing protein that regulates cell cycle by phosphorylation and dephosphorylation. It plays an important role in the proliferation and differentiation of cells, in addition to the tumorigenesis. This protein is highly expressed in a wide variety of human cancers, including leukemia and multiple types of solid tumors. The relationship between STMN1 and gastric cancer has recently been investigated. Studying STMN1 in gastric cancer is important. A number of studies have suggested that overex-pression of STMN1 can affect the therapeutic response of docetaxel, an anti-microtubule drug. This review summarizes the role of ST-MN1 in gastric carcinogenesis, development, prognosis, and treatment. The relationship between STMN1 and clinical pathology and its regulation pathways is also investigated.

Objective To investigate the inhibitory effect of Arg-Gly-Asp-Ser (RGDS) on proliferation and collagen synthesis stimulated by fibronectin (FN) and the influence on the expression of focal adhesion kinase of hepatic stellate cell (HSC), in order to provide a theoretical ground for its use in prevention and treatment of hepatic fibrosis in clinic. Methods The adhesion rates were determined by toluidine blue colorimetric assay. The effects of RGDS on the proliferation and collagen synthesis of HSC were assessed by ~3H-TdR and 3H-proline incorporation assays, respectively. The FAK mRNA of HSCs was determined by RT-PCR. Results RGDS was found to inhibit the rates of adhesion, while RGES has no such ability; ②The proliferation and collagen synthesis of HSC were inhibited by RGDS in dose and time-dependent manners; ③The expression of FAK in the (25, 50, 100mg/L) RGDS groups was significantly lower than that in the control group at mRNA level. Conclusions RGDS can inhibit proliferation and collagen synthesis of HSC, and these effects correlate with its anti-adhesion ability. Meanwhile the expression of FAK is also down-regulated significantly. As a signaling molecule, FAK may be involved in the effect of RGDS. This may be one of the underlying mechanisms of anti-fibrosis effect of RGDS.

Objective To explore the role of extracellular signal-regulated kinase(ERK)in the apoptosis of hepatic stellate cells(HSC) induced by Arg-Gly-Asp-Ser (RGDS) tetrapeptide. Methods The cultrued HSC cells were divided into six groups including untreated control, fibronertin (FN), FN+RGDS (25 mg/L), FN+RGDS (50 mg/L), FN+RGDS(100 mg/L) and FN+Arg-Gly-Glu-Ser(RGES, 100 mg/L) groups. 3H-thymidine incorporation, transmission electron microscopy and TUNEL were employed to estimate the influence of RGDS on proliferation and apoptosis of HSC. The HSC adhesion rates were observed by toluidine blue colorimetric assay. The expressions of ERK mRNA and ERK protein in HSC were detected by RT-PCR and Western blotting. Results Compared with control and fibronectin groups, RGDS tetrapeptide at concentrations of 25 mg/L, 50 mg/L and 100 mg/L inhibited the proliferation of HSC(P