Enhanced management of the entire process of medical wastes, ranging from sorting collection, delivery, transport to temporary storage: compilation of the computerized management system for medical wastes: development of quality control inspection standards for medical wastes: all-staff training for the awareness of legal compliance for medical wastes, prevention of medical wastes from jeopardizing both human being and the environment.

Objective: To investigate the management of NH~-_3 burned cases with inhalation injury. Methods:The clinic treatments of 15 NH~-_3 burned cases complicated with inhalation injury in his hospital between January 1998 and April 2004 were analyzed retrospectively.His management included reasonable fluid therapy,prompt and efficient usage of antibiotics ,tracheotomy after diagnosis,mechanical ventilation,bronchoalveolar lavage using fibrobrochoscope after the shock period and short-time usage of glucocorticoid. Results: Of the 15 cases,14 patients were cured and 1 patient was improved without any death.The cure rate was 93.3%. Conclusion: All of the managements should be performed as quickly as possible.Paying much attention to prevent the complications could improve the cure rate.Tracheotomy and bronchoalveolar lavage in the early stage are vital to patients.

Objective To investigate the inhibitory effect of RNA interference(RNAi)on WT1 gene expression in HL-60 cell line.Methods Several pairs of small interfering RNA(siRNA)for WT1 gene was designed and transfected into HL-60 cells.WT1 gene expression was assessed by RT-PCR,and WT1 protein expression was tested by Western blot.Flow cytometry was used to measure the cell apoptosis.Results The expression rates of WT1 mRNA and the expression rates of WT1 protein were(23.62%?1.28%),(23.20%?1.04%),respectively in HL-60 WT1 siRNA-transfected cells,significantly lower than those of control cells(P

ObjectiveTo discuss influence of Lamaze childbirth method on breast milk secretion during the course of Doula delivery. Methods106 primipara of natural childbirth were divided into the observation group(52 cases) and the control group(54 cases). Lamaze childbirth method of minus pain was applied in the observation group based upon Doula delivery, the control group only received Doula delivery. The initiating time of lactation, lactation volume were observed and the result underwent t test and χ2 test. ResultsThe initiating time of lactation was earlier and the lactation volume was increased in the observa-tion group than those in the control group. ConclusionsApplication of Lamaze childbirth method of mi-nus pain during the course of Doula delivery can promote early postpartum lactation and adequate lac-tation volume.

Objective To observe the effects of nose-stuffy continuous positive airway pressure(CPAP)combined with large-dose Mucosolvan on the hyaline membrane disease of newborn(NHMD). Methods Third),divided into three equal parts,by intravenous drop infusion]. According to the results of SpO2 and blood gas analysis,the CPAP setting was adjusted. Before and after the treatment,the SpO2、RR、PaO2、PaCO2、SaO2 of all the children were analyzed. Results Ten children of all(76.9%) were cured,two died,one quitThe SpO2 of the total,at one hour after the treatment,improved significantly(P＜0.001);the RR、PaO2、SaO2 at 24 hours later also improved(P＜0.05). Conclusion The method of nose-stuffy continuous positive airway pressure(CPAP) combined with large-dose Mucosolvan,which had good effects on ameliorating the symptom and improving the gas exchange,is worth used in primary hospitals widely.

Acute Coronary Syndrome ; etiology ; Anaphylaxis ; complications ; Humans

Objective Introducing the job embedded scale to explore the dropout intention of medical workers at tertiary hospitals.Methods Correlation analysis, analysis of variance and regression analysis were used to analyze with a job embedded scale, the dropout intention of medical workers of a Beijing hospital.Results There was a significant negative correlation between job embedding and dropout intention of such people(r=-0.223).The predictive ability of on-the-job embeddedness is better than off-the-job embeddedness.Fit-organizational, sacrifice-organizational, fit-community and sacrifice-community were significantly negatively correlated with dropout intention.There are differences in the relationship between job embedding and dropout intention of medical workers of different individual attributes.Conclusion Job embedded scale can effectively predict the dropout intention of medical workers, offering a usable tool to predict the dropout of these people.

Objective To investigate the inhibitory effect of Arg-Gly-Asp-Ser (RGDS) on proliferation and collagen synthesis stimulated by fibronectin (FN) and the influence on the expression of focal adhesion kinase of hepatic stellate cell (HSC), in order to provide a theoretical ground for its use in prevention and treatment of hepatic fibrosis in clinic. Methods The adhesion rates were determined by toluidine blue colorimetric assay. The effects of RGDS on the proliferation and collagen synthesis of HSC were assessed by ~3H-TdR and 3H-proline incorporation assays, respectively. The FAK mRNA of HSCs was determined by RT-PCR. Results RGDS was found to inhibit the rates of adhesion, while RGES has no such ability; ②The proliferation and collagen synthesis of HSC were inhibited by RGDS in dose and time-dependent manners; ③The expression of FAK in the (25, 50, 100mg/L) RGDS groups was significantly lower than that in the control group at mRNA level. Conclusions RGDS can inhibit proliferation and collagen synthesis of HSC, and these effects correlate with its anti-adhesion ability. Meanwhile the expression of FAK is also down-regulated significantly. As a signaling molecule, FAK may be involved in the effect of RGDS. This may be one of the underlying mechanisms of anti-fibrosis effect of RGDS.

OBJECTIVE:To discuss the pathways to enhance core competitiveness by the implementation of the Good Supply Practice(GSP)management in Medicine Chain Drug Stores.METHODS:To analysis from sequentiae several aspects: consummate the quality system,create the brand value and conversion the management philosophy in drug retail trade.RESULTS & CONCLUSIONS:Through the implementation of the GSP to create efficiency with management,create brands with quality and offer best after-sale service and customer service so as to enhance enterprises' core competitiveness.

AIM: To investigate the effect of IH764-3 on the apoptosis of H 2O 2-stimulated hepatic stellate cells(HSCs) and the alteration of focal adhesion kinase (FAK). METHODS: The proliferation of HSCs was examined by direct cell count and the apoptosis was determined by Annexin-V/PI labeling, while the morphological change was observed by light microscopy and transmission electron microscopy. In addition, FAK mRNA was detected by RT-PCR. RESULTS: H 2O 2 promoted the proliferation of HSCs. IH764-3 induced the apoptosis of HSCs in a dose-dependent manner. The HSC apoptotic rates of different groups were 6.35%,9.28%,15.10%,19.69%,respectively, after treated with different concentrations of IH764-3 for 48 h while H 2O 2 group showed 2.30%. In 30 mg/L group, the apoptosis rates were 6.73%?10.34%?15.10% for the indicated time periods(12 h, 24 h, 48 h). In the presence of IH764-3, FAK mRNA decreased. The FAK mRNA reduction began at 2 h after adding IH764-3. CONCLUSION: IH764-3 induced the apoptosis of HSCs. Down-regulating the expression of FAK mRNA may be one of its mechanisms.