Nephrotic syndrome,a common glomerular disease in pediatric department,is characterized by its long course of disease and facility to repeat or relapse,thus severely influences the physical and mental health of pediatric patients.Besides physical pains,most family members and patients show different degrees of mental stress towards this specific disease.Based on investigating clinical cases,we explored the features of mental stress among children with nephritic syndrome and their family members,and came up with corresponding psychological interference by effective communication skills between doctors and children with nephritic syndrome,offering humane concern,and proper instruction in clinical treatment in order to increase the compliance of children patients and their family members to medical treatment.Therefore,children patients and their family members manifest favorable attitude and cooperate with medical staff in the treating process,thus a satisfactory treating outcome will be achieved.

Objective To investigate the clinical characteristics and risk factors of neonatal hyperbilirubinemia.Methods The clinical data of 826 cases with neonatal hyperbilirubinemia were retrospectively analyzed.The jaundice appeared time,clinical characteristics were monitored,and the causes of jaundice appeared were analyzed.Results The jaundice cases at ≤ 1 day,＞ 1 ～ 7 days,more than ＞ 7 days were 71 cases,471 cases,284 cases,accounted for 8.60％,57.02％,34.38％ ;The top three risk factors of jaundice constitute were breast milk jaundice,perinatal factors,infectious factors,the cases were 215 cases,206 cases,200 cases,the proportions were 26.03 ％,24.94％,24.21％ ;The proportion jaundice of newborn caused by ABO hemolytic was 9.44％ ;There were 220 cases of 812 cases had neonatal jaundice,including 170 cases of full-term of breast milk jaundice,the proportion was 27.15％,50 cases of preterm infants of breast milk jaundice,accounted for 26.88％,there was no significant difference(P ＞ 0.05) ;78 cases was full-term breast milk jaundice,the proportion was 22.94％,120 cases of cesarean section appeared breast milk jaundice,accounting for 36.81％,the difference was statistically significant(P ＜ 0.05).Conclusion The degree of neonatal jaundice is affected by many factors,which related with individual differences,geographical,economic and cultural level,race,gender,feeding methods,genetic and so on,the strict observation and identification of neonatal jaundice and the establishment of supervision system and the protection for active and effective measures,taking active and effective treatment have very good effect to reduce hyperbilirubinemia complication as well as a good effect on the prevention and treatment of neonatal hyperbilirubinemia.

Aim To observe the effect of DL-buthionine s ulfoximine(DL-BSO) on the radiosensitivity of rat C 6 glioma cells under the a erobic and the hypoxic condition. Methods The source of radiati on was 60Co ?-rays. The rats C 6 glioma cells were treated by radia tion alone or DL-BSO+radiation under the aerobic and the hypoxic condition. Col ony forming assay was used to measure effects of DL-BSO on the radiosensitivity . Results Radiosensitive effect of DL-BSO was time-depedent u nder the aerobic condition. After treatment with 0.1 mmol?L -1 DL-BSO fo r 2, 6, 12 hours, the radiosensitive effect was not observed, whereas an enhance ment of radiosensitivity was seen at 24 and 48 hours. An enhancement of radiosen sitivity was seen at 2～48 hours after treatment with 0.1 mmol?L -1 DL-B SO under the hypoxic condition. The radiosensitive effects related to DL-BSO co ncentration under the aerobic and the hypoxic condition. Conclusion Both under the aerobic and the hypoxic conditions DL-BSO can increase the radio sensitivity of rat C 6 glioma cells. DL-BSO increased the rat C 6 gliom a cells radiosensitivity especially under the hypoxic condition, and radiosensit ive effect of DL-BSO is time and concentration-dependent.

Objective: To revise the Griffith Mental Development Scales and establish the norm being suited to the Chinese local conditions. Methods: The standardized sample consisted of 2022 children aged 0 to 7 years old was collected in a stratified and random way according to city rank, age, sex, paternal occupations. Each subject was tested individually and filled in a form. Results: The total sample had better representation. The mean of GQ was about 100, and the distribution of GQ is normal. Item analysis indicated that the item difficulty was about 0.5 and discrimination was from 0.62 to 0.78. A few orders of items were revised. The scorer reliability was 0.953. The test-retest reliability was 0.948. The correlation coefficients were 0.481 to 0.854 among the sub-scales or between sub-scales and General DQ. The result of exploratory factor analysis had three factors. The ratio of variance was 79.8%.Conclusion:Griffith Mental Development Scales have ideal reliability and validity.

Based on the hospital information systems and the hospital network,a software for patients with peripherally inserted central catheter (PICC) was developed and applied in clinical practice,which achieved the share of PICC information within the hospital,saved nursing time effectively,made the clinical data more standardized,systematical and objective.

Objective To study on the changes of the DNT cells and T lymphocyte subtype in children with infectious mononucleosis (IM) and its clinical significance.Methods The flow cytometry (FCM) was used to detected the DNT cells and other T lymphocyte subtype in 48 cases of IM.Results The study showed that DNT cells( 9.39 ± 4.89 )% were greatly increased in comparison with normal controls (NC) (4.26 ± 1.68)% ( P ＜0.01 ).CD4 cells(21.45 ±9.87)% were decreased ( P ＜0.01 ) and CD8 cells increased in comparison with NC (32.43 ± 5.07) % ( P ＜ 0.01 ).Conclusion DNT cells and T lymphocyte subtype can be used to evaluate the immune function of children with infectious mononucleosis (IM) and provide guidance for adoptive immunotherapy.

Objective To investigate the expression differences of MiR-34a/c in lesions of psoriasis vulgaris Uyghur and Han patients and beside-lesional tissue and normal skin tissue in XinJiang.Methods Real-time fluorescent quantitative polymerase chain reaction (PCR) was used to determine the expression levels of MiR-34a/c in the psoriasis vulgaris Uyghur and Han patients with lesion tissues, beside-lesion tissues, and normal skin tissues, respectively.Results The expression level of MiR-34a was higher in lesions of psoriasis vulgaris in XinJiang than in the beside-lesion tissues and normal skin tissues, and differences were statistical significance (P ＜ 0.05).However, the expression level of MiR-34a in beside-lesion tissues and normal skin tissues, and in the Uyghur and Han psoriasis vulgaris werent statistically significant difference (P ＞ 0.05).The expression level of MiR-34c in three types of tissues and in the Uyghur and Han psoriasis vulgaris weren't statistically significant difference (P ＞ 0.05).Conclusions MiR-34a might participate in the pathogenesis of Uyghur and Han psoriasis vulgaris in XinJiang.

OBJECTIVE To investigate the existence of genes for beta-lactam antibiotic resistance and for aminoglycosides modification enzymes(AMEs) in Pseudomonas aeruginosa(PAE) isolates from ICU patients and analyze the homology among strains.METHODS ?-Lactamase genes including TEM,SHV,OXA-10,PER,VEB,GES,CARB,IMP,VIM,SPM,GIM,DHA,FOX,MOX and oprD2,were detected by PCR amplication in 21 PAE isolates.The genes for AMES including aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(3″)-Ⅰ and ant(2″)-Ⅰwere determined by PCR amplification as well.RESULTS Among 21 isolates 21(100%),2(9.5%),1(4.8%),2(9.5%)and 4(19.0%) were positive for TEM,SHV,GES,CARB and VIM genes,respectively.The deletion of oprD2 gene was found in 14 out of 21 strains.Other ?-lactamase genes were absent in all isolates.As for AME genes,aac(3)-Ⅱ,aac(6″)-Ⅰ,aac(6)-Ⅱ,ant(3″)-Ⅰ,ant(2″)-Ⅰ and aac(3)-Ⅰgenes were present in 19.0%,23.8%,9.5%,4.8%,and 19.0% of 21 isolates,However,aac(3)-Ⅰ gene was no position in any isolates.CONCLUSIONS P.aeruginosa carries various beta-lactamase and AME genes in ICU patients.Genetic cluster analysis suggested that clonal propagation result in nosocomial infection of PAE.

Objective To study the antithrombotic effect of polysaccharide extracted from Thallus Laminariae(PTP) on rats with endothelial injury.Methods Two kinds of endothelial injured rat models were established by injecting adrenaline and endotoxin.The wet weight of thrombus was evaluated by artery-vein loop method,the starting time of thrombus formation and the speed of blood flow were observed by mesentery microcirculation.Results In adrenaline-induced injury rats,the wet weight of thrombus was obviously decreased in high-dose PTP group and aspirin group compared with the model group.In endotoxin-induced injury rats,the starting time of thrombus formation was longer and the one-hour blood flow speed was faster in all TP groups and aspirin group than those in the model group.Conclusion PTP exerts the antithrombotic effect on rats with endothelial injury.

OBJECTIVE To investigate genes associated with aminoglycosides modification enzymes(AMEs) in Pseudomonas aeruginosa(PAE) isolated from ICU patients.METHODS Drug-reisistant genes encoding AMEs such as aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(3″)-Ⅰ and ant(2″)-Ⅰ were detected by polymerase chain reaction(PCR)(amplification) in 21 PAE isolates.RESULTS The positive rates of aac(3)-Ⅱ,aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(3″)-Ⅰ,ant(2″)-Ⅰ and aac(3)-Ⅰ genes were positive in 19.0%,23.8%,9.5%,4.8%,19.0% and 0% of 21 isolates,respectively. Drug-resistant genes encoding AMEs were detected positively in 42.8% of 21 isolates.(CONCLUSIONS) AMEs genes are present in high percentage of PAE isolated from ICU patients.