Some measures have been taken such as adjusting validated experiment,establishing and launching an integrated designing experiment for biopolymers,circumfusing experimental correlational content,discussing the problem-based learning method,assorting home-made multimedia teaching material and combining proper motion designing biochemical experiment in a lot of biochemical experimental teaching to train the basic skills of the high-level talents in medicine.

Objective To assess the effect of the intensive insulin therapy for preventing the intensive care unit acquired weakness systematically ,in order to provide reference for making the intervention strategy .Methods We searched the database CBM ,CNKI ,WanFang Data ,VIP ,PubMed ,Cochrane Library ,EMBASE and ISI from the day of establishment to Dec 2014 to find the relevant studies with computer and by hand .Two researchers selected the studies ,evaluated the quality of the articles and ex‐tracted data according to the rigorous criterion ,then we performed the Meta‐analysis on the data with the software RevMan5 .3 .Re‐sults Three articles were enrolled ,2 788 patients in the intensive care unit were recruited ,including 1 380 patients were from the experimental group and 1 408 patients came from the controlled group .The result of the Meta‐analysis showed that compared to the conventional insulin therapy ,intensive insulin therapy reduced the incidence of intensive care unit weakness(RR= 0 .61 ,95% CI:0 .42-0 .89 ,P=0 .01) ,but there was no statistical significance of it on the the intensive care unit mortality(RR=0 .74 ,95% CI:0 .47-1 .17 ,P=0 .20) ,the hospital mortality(RR=0 .81 ,95% CI:0 .58 -1 .13 ,P= 0 .22) ,the duration of intensive care unit stay (MD=0 ,95% CI:-0 .37-0 .37 ,P=1 .00) and the duration of mechanical ventilation(MD= -1 .48 ,95% CI:-3 .43-0 .47 ,P=0 .14) . Conclusion Intensive insulin therapy can reduce the incidence of intensive care unit acquired weakness ,but the effect of it on the intensive care unit mortality ,hospital mortality ,duration of intensive stay and duration of mechanical ventilation needs further research to verify .

ObjectiveTo study the effects of painful inter-stimulus interval on event-related potentials in rabbits for best inter-stimulus interval.MethodsElectrode was attached to F4 site on rabbits' skull. An Oddball paradigm stimulus were adopted. The waveforms recorded under different inter-stimulus interval were analysed.ResultsThe latency of pain evoked potentials P2,N2,P3a under different inter-stimulus interval showed no significant difference.The amplitude was highest when the interval was 8s.ConclusionsThere is a best inter-stimulus interval for pain evoked potential.

Objective Discusstheinfluence of early diagnosis of myocardial damage and heart-type fatty acid binding protein(h-FABP)on patients with severe head injury after rehabilitation.Methods The sorum h-FABP was detected using enzyme-linked immunosorbent one-step quantitative detection,cTnI using solid-phase enzyme-linked immunosorbent assay(ELISA),CK-MB using the method of immunosuppressive.Results The sorum levels of h-FABP,cTnl and CK-MB in severe head injury patients levels were significantly higher than that of healthy control group(P<0.01),the positive serum h-FABP in severe head injury patients Was significantly higher than cTnI,CK-MB or ECG(P<0.01);8 cases died in47 cases of severe head injury patients,the mortalisty rate was 17.02%(8/47),the mortality rate of abnormal sentrum h-FABP,cTnI and CK-MB group was significantly higher than the normal group(P<0. 01);The serum h-FABP in predicting the death of severe head injury patients was high in sensitivity and negative predictive value,but lower in specificity and accuracy.Condusiom The serum h-FABP quantitative assay as a high-sensitive indicators to minor myocardial dsmage can be used an objective indicators forjudging severity ofacute severe head injury,evaluating incidence of accidents.

0.05), but it increased writhing times of mice (P0.05), but HPPT was decreased(P

0.05), while strychnine and naloxone obviously lengthened the sleeping time of the mice injected with thiopental sodium (P

Objective To evaluate the value of hyperbaric oxygen treatment on patients with traumatic brain injury and to explore the possibility of C-reactive protein(CRP) as a index for estimating the effect of hyperbaric oxygen treatment on patients with traumatic brain injury.Methods 70 patients with traumatic brain injury were divided into two groups by randomization:hyperbaric oxygen treatment group(group A) and control group(group B).The recovery of patients was evaluated with Glasgow Coma Scale(GCS),while the content of serum CRP in 70 patients was measured before and after a course of treatment.Results ⑴After a course of treatment,GCS of group A was (11 9?1 7) scores,while GCS of group B was(10 7?2 0) scores,there was significantly difference between two groups(P

Objective To explore the effect of labels management on high-risk drugs. Methods Labels management group was established, to be responsible for use and storage of high-risk drugs. The management methods included taking analysis on adverse nursing events, designing and making labels in drug storage and use, and making a field survey on adverse nursing events before and after the management. Result After manipulation of the labels management, the number of adverse nursing events of drug use fell from 35 to 0. Conclusion Drug storage and use with special, standard labels is effective in preventing nursing adverse events in high risk drugs.

Objective To develop neural stem cells(NSCs) which can stably express exogenous brain-derived neurotrophic factor(BDNF) in vitro. Methods NSCs from the subependymal zone of embry-onic day 14.5(E14.5) rat brain were purified by limiting dilution assay and then infected with supernatant of recombinant retrovirus pLXSN-BDNF and retrovirus pLXSN. The original copy numbers of exogenous gene templates from three groups NSCs(pLXSN-BDNF viral infection group, pLXSN viral infection group, control group) were detected by fluorescent quantitative PCR(FQ-PCR). ELISA assay was used for determining the protein contents of BDNF of supernatant from three groups NSCs for six days continually after seeded in 24-well plates in the same cell density. Results NSCs were purified successfully by limiting dilution assay.The original copy numbers of exogenous BDNF gene templates from pLXSN-BDNF viral infection group by FQ-PCR were (19.57±0.65) × 10~3 copies/μl, higher than those of another two groups(P < 0.05). The protein contents of BDNF of supernatant from NSCs of pLXSN-BDNF viral infection group was highest among three groups and compared with another two groups had statistical significance (P <0.05) . Conclusion The purified NSCs can be transduced exogenous BDNF successfully with supematant of recombinant retrovir-us pLXSN-BDNF which provide experimental evidences and laying foundations for further research of retinal transplantation and quantization investigation of gene therapy for optic nerve injury.

Objective To investigate the efficiency and safety of transfection green fluorescent protein plasmid to mouse cornea mediated by SonoVue and ultrasound.Methods Saline,plasmid+saline,plasmid+SonoVue and liposome+plasmid were injected respectively to mouse eye anterior chamber.Then the mouse eye of plasmid+saline and plasmid+SonoVue injection group were exposed to pulse wave ultrasound under 50 Hz pulse reDetition frequence,2 W/cm2 intensity and 10 minutes duration time.Fluorescence stereomicroscope was used to observe the expression of EGFP in the eye at the 1 st day,3rd day,7th day,14th and 21 st day after injection.Two mice were taken randomly from each group and were sacrificed at the 3rd day after injection.Their eyes were enucleated and made into frozen coronal sections.And fluorescence microscopy was performed to observe the type and distribution of EGFP positive cell.Tissue damage was observed in pathological section.Results EGFP was exDressed over the ocular surface in SonoVue and ultrasound group,and it obviously higher than only ultrasound exDosure group and liposome group.The expression of EGFP was not detect ed over the ocular surface in other groups.Pathological sections were not found any difference in each group.Conclusions SonoVue and ultrasound can successfully and safely transfer gene to ocular in vivo study.