As China is the birthplace of traditional Chinese medicine, herbal compound is an important part of Chinese traditional culture, and is the field with China’s most obvious advantage and national features to participate in international competition. This article provided several suggestions according to the compound patent application document drafting including specification,claim of rights, and“novelty, creativity, practicability”investigation angle in order to enhance quality of herbal compound patent writing.

OBJECTIVE:To promote the rational use of anti-cold drugs in primary hospital. METHODS:The anti-cold drugs commonly used in outpatient pharmacy of Chinese and western of our hospital were investigated to summarize and analyze the west-ern drugs and Chinese patent drugs. RESULTS:There were totally 33 anti-cold drugs,including 12 kinds of western drugs and 21 kinds of Chinese patent drugs. Mostly anti-cold western drugs were compound preparations,mainly including 5 ingredients:anti-in-flammatory,vasoconstrictor,antitussive,antihistamine and expectorant;the wind-cold common cold and wind-heat common cold should be differentially treated and the role of the characteristics should be grasped. CONCLUSIONS:Physicians and pharmacists should fully understand and grasp the composition,content,indications,the role of the characteristics,adverse reactions and pre-cautions. Different drugs should be given corresponding medication guide based on the clinical symptoms of the patients.

OBJECTIVE:To upgrade and achieve the management targets of drug storehouse. METHODS: Based on the tasks for drug storehouse, the application of THIS System in the management of drug storehouse was studied. RESULTS: The application of THIS System in management of drug storehouse helps transform traditional manners and improve work efficiency and quality. CONCLUSION:The flexible application of current technique and improvement of information system is an important approach to achieve work efficiency and quality as well as the management targets of drug storehouse.

Objective To observe indicators of hemorheology and blood gas analysis of COPD( chronic obstructive disease) patients complicated by CI(cerebral infarction) in acute exacerbation time and the relationship between them.Methods 46 patients with COPD complicated by CI in acute exacerbation time(complications group) and 44 patients with COPD in acute exacerbation time(COPD group) blood rheology indicators,fibrinogen(Fg) ,D-di-mer(DD) and blood gas analysis were detected.And 45 cases of health-related physical check (control group) were recruited for comparison.Results Compared with the control group,complication group,COPD group of whole blood viscosity,plasma viscosity,hematocrit,platelet adhesion rate,Fg,and DD levels were significantly increased,the difference was significant(P<0.05) ;The complications group of whole blood viscosity,Fg,and DD levels were significantly higher than COPD group;Fg and DD and blood PaCO2 correlated( r Fg =0.36,P<0.01 ,rDD =0.510,P<0.01).Conclusion Patients with chronic obstructive pulmonary disease and cerebral infarction in acute exacerbation time had abnormal coagulation status,and improving ventilation and early application of anticoagulant drug therapy,could reduce the chronic obstructive pulmonary disease,the occurrence of cerebral infarction.

MicroRNA (miRNA) is a class of noncoding small-molecule RNA in eukaryotic cells and certain viruses,which plays a major role in the post-transcriptional level of target-gene regulation.Due to their nonimmunogenic nature,viral miRNA represents an elegant tool for the virus to evade the host immune system,and is likely to play a key role in the latent/Iytic switch during the viral lifecycle.Human cytomegalovirus(HCMV) is a widespread herpesvirus.HCMV-encoded miRNAs play an improtant role in lantent infection by targeting viral and cellular genes.These miRNAs have the potential to become novel biomarkers and targets of treatment.This review summarizes the recent advances of HCMV-encoded miRNA.

Objective To study the clinical significance of color Doppler ultrasound in diagnosis the carotid atherosclerotic plagues and medicine intervention. Methods Give ultrasound examination in the carotid artery to the outpatients and inpatients with hypertension, hypedipemia or diabetes, who has pathological change in the carotid artery,and 62 cases of them are atherosclerotic change using medicine intervention. Results Ultrasound may show accurately the size and positions of atheroma, which include fatty atheroma,soft atheroma,stiff atheroma and mixed atheroma. After taking the medicine,in the first period, the diameter of theatheroma shrank in a broad range, from ( 1.82 ± 0.45 )mm to ( 1.53 ± 0.42 ) mm. And the second period, it can shrink in a small range, from ( 1.53 ± 0.42 )mm to ( 1.32 ± 0.40) mm. Conclusion Ultrasound examination can be regarded as the asoreferred method in diag- nosis of the carotid atherosclerotic plague and the monitor of medicine intervention.

Through researching YE Tian-shi’s theory source,the point of therapeutic methods of Qi collateral asthenia syndrome and collaterals diseases,attaching importance to physique factor and treating in accordance with seasonal conditional,this thesis expatiated intensively YE Tian-shi’s theory of therapeutical principle and medication trait for Qi collateral diseases.Then enumerated the prescriptions in common usage for Qi collateral diseases which were well-chosen from YE Tian-shi’s medical record.In a way,this thesis embodied the academic thought of paying particular attention to Qi collateral therapeutic methods in YE Tianshi’s theory very well.

Some measures have been taken such as adjusting validated experiment,establishing and launching an integrated designing experiment for biopolymers,circumfusing experimental correlational content,discussing the problem-based learning method,assorting home-made multimedia teaching material and combining proper motion designing biochemical experiment in a lot of biochemical experimental teaching to train the basic skills of the high-level talents in medicine.

AIM: To establish chromatogram figerprints of fresh Magnoliae officinalis and their processed product by HPLC-DAD-MS method. METHODS: A HPLC-DAD-MS method was proposed for quality assessment of Magnoliae officinalis and their processed product,the HPLC columm,mobile phase,elution mode and gradient program were optimized in order to obtain high quality HPLC profile.HPLC analysis was performed on a LC-18 colunm(5 ?m,250 mm?4.6 mm) with the mixture of H_2O(A) and acetonitrile(B) as mobile phase in gradient mode.The colunm temperature was set up at 30 ℃ and the flow rate was 1 mL/min. RESULTS: The HPLC fingerprint was established based on the analysis of ten fresh Magnoliae officqinalis herbal samples.Twenty-two characteristic peaks were found in all ten samples.The main characteristic peaks were identified based on the comparison of UV and MS spectra of each analyte with that of authentic compounds and literature data.The HPLC fingerprint of processed Magnoliae officinalis was established.Comparing with corresponding fresh herbal samples,twent-three characteristic peaks were found in all ten processed herbal samples.Moreover,gingerol was found in processed Magnoliae officinalis. CONCLUSION: The fingerprint chromatograms from HPLC method can be used to evaluate the quality of Magnoliae officinalis and their processed products.

Objective:To investigate the expression of Bcl-2 in rat brain during dependence and withdral state.Methods:The rats were rendered dependent by subcutaneous injections of increasing doses of morphine from 10 to 80 mg/kg for 5 consecutive days.The rats as controls were administered with same volume of normal saline.The expression of Bcl-2 mRNA was assayed with digacin-labelled Bcl-2 cDNA probe by in situ hybridization and the levels of Bcl-2 protein were measured by immunohistochemistry.Results:The evels of Bcl-2 and their mRNA in prefrontal cortex,locus coeruleus,hippocampus and ventral tegmental area significantly decreased as comparied with that in control rats after chronic administration of morphine and naloxone administration.However,Bcl-2 and their mRNA in nucleus accumbens of rats in withdral state were markedly higher than that in nucleus accumbens of morphine dependent rats.Conclusion:Chronic exposure to morphine appears to induce modification of the expression of Bcl-2 mRNA and their protein of rats in the rat brain,which contributes to the development of morphine dependence.