MicroRNA (miRNA) is a class of noncoding small-molecule RNA in eukaryotic cells and certain viruses,which plays a major role in the post-transcriptional level of target-gene regulation.Due to their nonimmunogenic nature,viral miRNA represents an elegant tool for the virus to evade the host immune system,and is likely to play a key role in the latent/Iytic switch during the viral lifecycle.Human cytomegalovirus(HCMV) is a widespread herpesvirus.HCMV-encoded miRNAs play an improtant role in lantent infection by targeting viral and cellular genes.These miRNAs have the potential to become novel biomarkers and targets of treatment.This review summarizes the recent advances of HCMV-encoded miRNA.

Objective To observe indicators of hemorheology and blood gas analysis of COPD( chronic obstructive disease) patients complicated by CI(cerebral infarction) in acute exacerbation time and the relationship between them.Methods 46 patients with COPD complicated by CI in acute exacerbation time(complications group) and 44 patients with COPD in acute exacerbation time(COPD group) blood rheology indicators,fibrinogen(Fg) ,D-di-mer(DD) and blood gas analysis were detected.And 45 cases of health-related physical check (control group) were recruited for comparison.Results Compared with the control group,complication group,COPD group of whole blood viscosity,plasma viscosity,hematocrit,platelet adhesion rate,Fg,and DD levels were significantly increased,the difference was significant(P<0.05) ;The complications group of whole blood viscosity,Fg,and DD levels were significantly higher than COPD group;Fg and DD and blood PaCO2 correlated( r Fg =0.36,P<0.01 ,rDD =0.510,P<0.01).Conclusion Patients with chronic obstructive pulmonary disease and cerebral infarction in acute exacerbation time had abnormal coagulation status,and improving ventilation and early application of anticoagulant drug therapy,could reduce the chronic obstructive pulmonary disease,the occurrence of cerebral infarction.

Objective To study the clinical significance of color Doppler ultrasound in diagnosis the carotid atherosclerotic plagues and medicine intervention. Methods Give ultrasound examination in the carotid artery to the outpatients and inpatients with hypertension, hypedipemia or diabetes, who has pathological change in the carotid artery,and 62 cases of them are atherosclerotic change using medicine intervention. Results Ultrasound may show accurately the size and positions of atheroma, which include fatty atheroma,soft atheroma,stiff atheroma and mixed atheroma. After taking the medicine,in the first period, the diameter of theatheroma shrank in a broad range, from ( 1.82 ± 0.45 )mm to ( 1.53 ± 0.42 ) mm. And the second period, it can shrink in a small range, from ( 1.53 ± 0.42 )mm to ( 1.32 ± 0.40) mm. Conclusion Ultrasound examination can be regarded as the asoreferred method in diag- nosis of the carotid atherosclerotic plague and the monitor of medicine intervention.

As China is the birthplace of traditional Chinese medicine, herbal compound is an important part of Chinese traditional culture, and is the field with China’s most obvious advantage and national features to participate in international competition. This article provided several suggestions according to the compound patent application document drafting including specification,claim of rights, and“novelty, creativity, practicability”investigation angle in order to enhance quality of herbal compound patent writing.

Objective To investigate the clinical effects of artificial tear gel combined with diclofenac sodium in the treatment with xerophthalmia.Methods 110 patients with xerophthalmia were chosen as study subjects,and they were divided into research group(artificial tear gel + diclofenac sodium)and control group(artificial tear gel).BUT,FS,SIT,mental statue,symptom score and clinical effects after 20 days were compared between the two groups.Results The total effective rate of research group was 90.48%,which of the control group was 75.24%,the difference was statistically significant(x2=8.582,P<0.05).The FS[(0.87±0.15)points vs.(1.53±0.84)points]of the research group was lower than that of the control group after treatment,and the BUT[(9.86±1.13)s vs.(5.71±1.27)s],SIT[(6.85±1.59)mm/5min vs.(4.38±2.07)mm/5min]of the research group were higher than those of the control group,the differences were statistically significant(t=7.926,21.352,9.697,all P<0.05).After treatment,the symptom score of the research group was lower than that of the control group[(8.39±3.24)points vs.(12.85±3.42)points],the difference was statistically significant(t=9.701,P<0.05).The SDS score [(37.16±5.63)points vs.(56.49±6.14)points]and SAS score[(35.24±5.38)points vs.(54.30±6.42)points]of the research group were lower than those of the control group after treatment,and the differences were statistically significant(t=23.777,23.317,all P<0.05).Conclusion Artificial tear gel combined with diclofenac sodium can rapidly improve the clinical symptom of xerophthlmia and treatment effect.

Some measures have been taken such as adjusting validated experiment,establishing and launching an integrated designing experiment for biopolymers,circumfusing experimental correlational content,discussing the problem-based learning method,assorting home-made multimedia teaching material and combining proper motion designing biochemical experiment in a lot of biochemical experimental teaching to train the basic skills of the high-level talents in medicine.

OBJECTIVE To investigate drug-resistance status of Klebsiella pneumoniae(KPN) among oil field staff-workers and provide the evidence for clinical application of antibiotics.METHODS Referring to the National Clinical Laboratory Operation Rules,KPN strains were isolated and identified.The drug-sensitivity testing was performed by K-B methods.The extended-spectrum-?-lactamases(ESBLs) producers were detected by double-disc synergy test and disc confirming test.RESULTS The 276 strains of KPN were mainly isolated from respiratory tract and urinary tract.The isolating rate of ESBLs-producing KPN was 37.0%.The results of susceptibility testing showed the drug-resistance to common antibiotics in ESBLs producers was generally higher than that in ESBLs nonproducer.There was no KPN resistant to imipenem and meropenem.CONCLUSIONS Drug-resistance status of KPN in a oil field hospital is very serious.We should strengthen monitoring and controlling of it.

OBJECTIVE To approach the distribution and drug resistance of Pseudomonas aeruginosa(PAE) in nosocomial infection in intensive care unit(ICU) and provide the evidence for clinical controlling of it.METHODS Clinical data of nosocomial infection with PAE isolated from ICU from Jan 2005 to Dec 2008 were investigated retrospectively.The results of susceptibility test in 277 PAE strains were analyzed statistically.RESULTS PAE strains were mostly isolated from respiratory tract(56.0%).The drug resistance rates to ceftriaxone,cefotaxime and gentamicin were all above 64.0%.PAE was resistant to the other antibiotics in different degrees.The suceptibility rates to imipenem,meropenem,amikacin,cefoperazone/sulbactam,piperacillin/tazobactam and levofloxacin were all above 74.0%.CONCLUSIONS The clinician should use the antibiotics reasonably according to etiological testing to elevate the level of anti-infection treatment.

OBJECTIVE:To promote the rational use of anti-cold drugs in primary hospital. METHODS:The anti-cold drugs commonly used in outpatient pharmacy of Chinese and western of our hospital were investigated to summarize and analyze the west-ern drugs and Chinese patent drugs. RESULTS:There were totally 33 anti-cold drugs,including 12 kinds of western drugs and 21 kinds of Chinese patent drugs. Mostly anti-cold western drugs were compound preparations,mainly including 5 ingredients:anti-in-flammatory,vasoconstrictor,antitussive,antihistamine and expectorant;the wind-cold common cold and wind-heat common cold should be differentially treated and the role of the characteristics should be grasped. CONCLUSIONS:Physicians and pharmacists should fully understand and grasp the composition,content,indications,the role of the characteristics,adverse reactions and pre-cautions. Different drugs should be given corresponding medication guide based on the clinical symptoms of the patients.

OBJECTIVE:To upgrade and achieve the management targets of drug storehouse. METHODS: Based on the tasks for drug storehouse, the application of THIS System in the management of drug storehouse was studied. RESULTS: The application of THIS System in management of drug storehouse helps transform traditional manners and improve work efficiency and quality. CONCLUSION:The flexible application of current technique and improvement of information system is an important approach to achieve work efficiency and quality as well as the management targets of drug storehouse.