Objective To study the efficiency of endoscope-guided Savary bougie dilatation ( ESBD ) for strictured duodenal ulcer. Methods Forty-five patients with strictured duodenal ulcer were treated with endoscope-guided Savary bougie dilatation. The items of investigation were; easiness of passing the endo-scope through the strictured portion of duodenum, resolving of their symptoms and the complications happened after dilatation. Results The symptoms remitted and endoscope was advanced to descending portion of the duodenum through the stricture in all of 45 patients. The follow up period ranged from 6 months to 36 months. The rates of symptomatic remission were 97. 8% (44/45 ) , 97. 8% (44/45 ) , 94. 4% (34/36) , 93.7%(30/32) , 91.7% (22/24) and 87.5% (14/16) , at 6,12,18,24,30 and 36 months respectively. Recurrence of symptoms in patients without therapy was significantly higher than those with therapy. Conclusion ESBD is a safe and efficient procedure in treating the strictured duodenal ulcer.

Objective To investigate the diagnostic value of laparoendoscopic exploration and peri-toneum biopsy in patients with ascites of unknown origin and to study the feasibility of replacing hard laparo-endoscope with ultrathin gastroendoscope. Methods A total of 35 patients with ascites of unknown origin underwent laparoendoscopic exploration and biopsies were taken at suspected sites on peritoneum. Results Definite diagnosis was made in 33 patients after laparoendoscopy (94. 3% ), among which there were 10 tuberculosis peritonitis (28. 6% ), 7 metastatic peritoneal carcinoma (20%), 5 liver cancer, 3 liv-er cirrhosis, 3 primary peritoneal carcinoma, 2 ovary cancer, 1 hepatovascular sarcoma, 1 spleen lymphoma and 1 acidophilic enteritis. No procedure related complications were observed. Conclusion Laparoendosco-py is safe and effective in target peritoneal biopsy, and is of great importance in diagnosis of ascites of un-known origin.

Objective:To study the effects of hyperthermia on apoptosis in human colonic cacinoma cell line Lovo. Methods:Lovo cells were exposed to hyperthemia and apoptosis was measured with fluorescent Hocehst 33258 staining, electropherogram gel, flow cytometry (FCM). Results:Significant changes in cell morphology , apoptosis peak apeared before the G1 phase and DNA ladder was observed in various group cells which were exposed to different temperature and in different time. Most significent changes were observed in cells exposed to 43℃ for 3,4 h and 44℃ for 1,2 h. Also cell cycle was found changed. Conclusion:Hyperthermia can cause apoptosis in human colonic cacinoma cell line Lovo, and has phase-specificity.

0.05). Significant difference existed between the patients and endoscopy-negative (pathological reflux) and endoscopy-negative (physiological reflux) (P

0.05).Conclusions:Laser welding joint gap at 0.00~1.00 mm does not affect the mechanical property of laser-welded titanium.

AIM: To study the enhancing effect of penetration enhancer on percutaneous absorption of antifungal agents. METHODS: Percutaneous penetration experiment was performed on device in vitro, and the deposit effects were observed with vivoperception. RESULTS: Combination of 1-menthol (1%) and azone (1%) showed the best effect on percutaneous absorption of cidopicox olamine, and 1-menthol (1%) had the best effect on percutaneous absorption of salysic acid. The compound penetration enhancer, consisting of 1-menthol (1%), azone (1%), propylene glycol ( 2.5%) and oleic acid ( 2.5%), had prominent action on absorption of cidopirox olamine through rabbit skins, and shorten the lag times obviously. CONCLUSION: L-menthol and azone can significantly enhance the percutaneous absorption of both salysic acid and cidopicox olamine. Combination of 1-menthol and azone can further increase the absorption.c

AIM To study the effects of ASA on proliferation and apoptosis of human lung Adenocarcinoma cell lines SPCA-1 in vivo. METHODS Cytotoxicity assay was tested by MTT method.Cell cycle was analyzed by flow cytometry(FCM).The morphology of the treated cells was observed by wright exclusion,Hoechest/PI exclusion, electron microscope. Apoptosis landder was evaluated by agarose gel electrophoresis of DNA. RESULTS ASA inhibited SPCA-1 cell proliferation in a time-and dose-dependent fashion(1.0～12.5 mmol?L -1,24 h～72 h). ASA increased the number of cells in G 0/G 1 and G 2/M phases,degrased the population of S phases at on 24 h and incresed apoptosis cells number. CONCLUSION ASA may inhibit the proliferation of SPCA-1 cell lines through effects on cell cycle and apoptosis.

Objective To investigate endoscopic clipping in treating acute gastroduodenal perforation. Methods Endoscopic clipping was carried out in 22 patients with acute gastroduodenal perforation as the complication of peptic ulcer or endoscopy. Results The clipping was successful in 19(86. 4% ) of 22 patients with acute gastro duodenal perforation, and surgical therapy was performed in 3(13. 6% ) of 22 cases. A single clipping was performed in 14(73. 7% ) of 19 patients resulted in satisfactory closure of perforation. An additional clipping was needed in 5(26. 3% ) patients 24 h after the first endoscopes procedure. The effectiveness of endoscopic clipping was related to location, cause and size of the perforation. Conclusion Endoscopic clipping is an effective method for acute gastroduodenal perforation as the complication of peptic ulcer or endoscopy.

Objective To investigate the expression and clinical significance of RegⅣand EGFR,PI3K proteins in gastric adenocarcinoma .Methods S-P immunohistochemistry was used to detect the expression of RegⅣand EGFR,PI3K proteins in pathological tissues of 73 cases with gastric adenocarcinoma and tumor -adja-cent normal gastric tissues .Results The positive expression rates of RegⅣand EGFR,PI3K proteins in 73 cases with gastric adenocarcinoma tissue were 50.7%(37/73),56.2%(41/73) and 69.9%(51/73) respectively, which were significantly different from the positive expression rates of tumor -adjacent normal gastric tissues ,be-ing 20.5%(15/73),19.2%(14/73),and 21.9%(16/73),respectively(P<0.05).The expression of RegⅣprotein was significantly correlated to differentiation degree ( P<0.05 ) and the expression of EGFR protein was significantly correlated to infiltrative depth,TNM stage,and lymph node metastasis(P<0.05).The expression of PI3K protein was significantly correlated to differentiation degree ,infiltrative depth,TNM stage and lymph node metastasis(P<0.05).There was positive correlation between the expressions of RegⅣ/EGFR,RegⅣ/PI3K and EGFR/PI3K proteins in gastric adenocarcinoma and the values of Spearman coefficient correlation were 0.325, 0.403 and 0.384,respectively(P<0.05).Conclusion RegⅣmay play an important role in gastric adenocarci-noma genesis and progression by activating EGFR /PI3K/Akt signaling pathway .

Objective To apply the techniques of CAD/CAM in the repair of defects and correction of deformity on the jaw bones so as to improve the accuracy and controllability of operations.Methods With CAD techniques,3D bone model was reconstructed based on CT images.Then,a repairing model was created with mirror operation on original model.The physical models of original model and repairing model were fabricated through rapid prototyping.Based on physical models,operation plan and model surgery with pre-forming plate could be designed.Results The designed operation plan and pre-forming plate techniques of CAD/CAM were successfully applied in eight patients,with satisfactory results.Conclusions With techniques of CAD/CAM,reconstructing and fabricating the original bone model and mirror repair model are very helpful for designing operation plan for maxillofacial surgery.It also can improve the surgery accuracy and save operation time obviously.