Objective To investigate the semen variation of the uremia patients after renal transplantation. Methods The semen samples from 10 uremia patients before (group A) and after (group B) renal transplantation and 12 normal volunteers(group C) were analyzed,and the main semen parameters of the 3 groups were compared. Results The mean sperm motilities of groups A,B and C were (13.8? 2.8 )%、(48.3?7.2)% and (63.8?3.6)%,respectively,the vitalities were (24.2?4.1)%、(76.3?3.9)% and (80.4?2.2)%,and the normal sperm morphologies were (15.6?2.3)%、(17.7?1.9)% and (33.8?3.7)%.The sperm motility and vitality of group B were significantly improved than group A(both P 0.05). Conclusions The successful renal transplantation can improve the semen parameters of the uremia patients.

Objective To explore the regularities and rules of compound herbal formulae for nonalcoholic fatty liver disease (NAFLD).Methods Compound herbal formulae on NAFLD published in journals of CNKI (1979.1-2014.1), VIP (1989.1-2014.1) and CBMdisc (1979.1-2014.1) were analyzed with association rule, frequency analysis and cluster analysis.Results In 107 prescriptions, 179 herbs were involved, and frequency of usage of those herbs was 1499. A cluster analysis was used to analyze 37 herbs with high frequency (≥10) of usage, which were classified into 9 clusters including promoting circulation, clearing heat herb, diuretic herb, food stagnation herb, regulating qi herb, phlegm-resolving herb, qi tonics herb, yin-nourishing herb, qi-activating herb and purgating herb. 30 pairs of herbs and 33 rules for NAFLD were obtained by association rule analysis.Conclusion It was a point of view to understand the regularities and rules of Chinese herb prescription on NAFLD by data mining.

Objective To investigate therapeutic effects of endoscopic mucosal resection( EMR) on gastric polyps and colorectal polyps.Methods 172 cases of elderly patients with early gastric and colorectal polyps were col-lected.They were divided into the observation group and control group by random number table,each group 86 cases. The observation group were treated with EMR,the control group were received conventional treatment for surgery.The clinical efficacy and adverse events were compared.Results The total effective rate of the observation group was 96.51%,significantly higher than 70.93% in the control group (χ2 =15.73,P<0.05);the postoperative adverse reactions in the observation group was 22.09%,significantly lower than 47.67% in the control group (χ2 =31.65, P<0.05).Conclusion For patients with pre-senile and senile stomach and colon polyps,the application of endo-scopic mucosal resection in the treatment not only can achieve better clinical efficacy, but also lower incidence of adverse reactions.

Objective To explore the safety, feasibility, and techniques of hand-assisted laparoscopic splenectomy (HSLS) for massive splenomegaly. Methods Between January 2005 and December 2006, 40 patients with massive splenomegaly owning to portal hypertension were treated with HSLS or open splenectomy (OS). The two groups were comparable in age, sex, hepatic function by Child classification, and size of the spleen. Results No serious complications occurred in both the groups. Compared with the OS group, patients in the HALS group had more blood loss [ (312?61) ml vs (235?105) ml, t=2.583, P=0.014], longer operation time [(95?20) min vs (73?16) min, t=3.832, P=0.000], earlier recovery of intestinal function [(48?1) h vs (98?1) h, t=-153.093, P=0.000], and shorter postoperative hospital stay [(6?2) d vs (10?2) d, t=-6.124, P=0.000]. Conclusions HSLS is feasible and safe for patients with massive splenomegaly. Despite a longer operation time, the procedure is superior in postoperative recovery.

Objective To observe the expression of vascular endothelial growth factor receptor (KDR)in human cervical carcinomas, to clarify the relationship between the expression and invasion, metastasis, prognostic value in human cervical carcinoma. Methods KDR was stained immunohistochemically in 80 cases of human cervical carcinoma. Results The expression of KDR in the endothelial cells of tumor vasculature is: the high KDR expression rate of clinical cancer stages ≤Ⅰb and Ⅱa～Ⅲa was 21 4% and 60 6% (P

Objective To evaluate the feasibility and clinical efficacy of minimally invasive surgery combined with sentinel lymph node (SLN) biopsy for early gastric cancer (EGC).Methods The clinical data of 39 EGC patients undergoing minimally invasive surgery combined with sentinel lymph node biopsy from December 2008 to January 2014 were analyzed retrospectively.Results 36 SLN negative EGC patients received endoscopic submucosal dissection (ESD), and 3 SLN positive EGC patients underwent laparoscopic distal gastrectomy with D2 dissection.The mean number of SLN was (2.8 ± 1.0).The SLN detection rate and accuracy of determination of lymph node (LN) status were both 100％.The rate of LN metastasis in patients with EGC was 8％ with no false negative rate.In ESD group, local recurrence and liver metastasis developed in one each cases.Meanwhile 1 patient in laparoscopic group sufferd postoperative liver and lung metastasis.Total disease free survival was 95％.Conclusions Minimally invasive surgery combined with sentinel lymph node biopsy for EGC is both safe and feasible.

BACKGROUND:Longitudinal study of changes in obesity is an important method to explore the etiology, which can provide scientific basis for preventing and control ing obesity.
OBJECTIVE:To investigate the effect of age, observation period and birth cohort on the obesity prevalence of adults in Jiangsu province through the age-period-cohort analysis.
METHODS:20-69-year-old adults in Jiangsu province were col ected as the research objects. The stratified cluster sampling method was used to col ect the obese data in 2000-2010, and analyzed with SAS software. RESULTS AND CONCLUSION:Obesity prevalence of 1946-1950 birth cohort to 1976-1980 birth cohort was gradual y increased (P<0.05) from 2000 to 2010. Obesity prevalence from 1931-1935 birth cohort to 1941-1945 birth cohort was not significantly increased (P>0.05). With the increasing age in each age group of over 25 years old, the risk of obesity was increased gradual y. There were significant differences in the odds ratios between the baseline groups of 20-25 years old and the age groups of over 25 years (P<0.05). Compared with the 1995-1999 birth cohort, the other periods had a slight influence on the obesity prevalence (P>0.05). Compared with 1976-1980 birth cohort, there were no significant differences in the risks of obesity of the birth cohorts after 1951-1955 (P>0.05). But the risk of obesity from 1946-1950 birth cohort to 1931-1935 birth cohort was gradual y significant since the 2000;obesity risks of those born in the different times were different;rural area wil be the key area than 45 years old.

Objective To study clinical diagnosis value of using magnifying endoscopy with narrow-band imaging(NBI-ME) in the CEA positive cases of microvascular parting to gastric cancer and colorectal cancer .Methods 873 cases in our hospital from 2012~2013 ,who had serum CEA positive were choosen ,the 114 patients who first diagnosis for suspected gastric cancer ,35 pa-tients who first diagnosis forsuspected colorectal cancer were included in this study .Using NBI-ME to check and the pathology bi-opsy .NBI-M E check key observation microvascular properties and classification :rules ,slightly irregular ,irregular ,disappeared ,the microvascular parting situation compared with pathological findings .Results 13 patients diagnosed with cancer by pathology ,the positive rate was 8 .72% ;intraepithelial neoplasia in 10 cases ,the positive rate was 6 .71% .No cases of cancer were regular and slightly irregular type ,all cancer cases in the disappearance .The sensitivity of type of disappeared was 100 .00% ,and the sensitivity of irregular type of canceration possibility intestinal large atrophy and high grade intraepithelial neoplasia was 87 .50% ,the differ-ence was statistically significant(P<0 .05) .Conclusion Using NBI-ME microvascular classification diagnosis could help observa-tion CEA positive cases in the screening of gastric cancer and colorectal cancer .

Objective To study the effect of the serum testosterone/estradiol ratio (T/E2) alteration on sperm defect and fertility. Methods The testosterone, estradiol, FSH, LH, PRL,sperm parameters and sperm morphology of 90 men were analyzed and the T/E2 and multiple anomalies index (MAI) were calculated. The patients were divided into 3 groups; T/E2≤10 (Ⅰ), T/E2＞10 (Ⅱ), and T/E2＞20 (Ⅲ). Results The sperm concentration and motility among the 3 groups were not significantly different (P＞0. 05). The percentages of the sperm whose head, neck and tail were abnormal declined gradually with the increase of the T/E2 in serum. The percentage of sperm head defeet of group Ⅰ was significantly higher than that of group Ⅲ (t=2. 482, P=0. 016) and that of sperm neck defect of groups Ⅰ and Ⅱ were significantly higher than that of group Ⅲ (t=4.113, 2. 050, P=0.000, 0. 046, respectively). The percentage of sperm tail defect among 3 groups was significantly different (t=2. 722, 3. 996, 3. 110, P=0. 008, 0. 000, 0. 003, respectively). The SDI of group Ⅱ was significantly higher than that of group Ⅰ (t= -2. 293, P= 0. 025). But the TZI increased gradually with the increase of the serum T/E2 and the TZI of groups Ⅱ and Ⅲ were significantly higher than that of group Ⅰ (t=2. 285, 2. 727, P=0. 025, 0. 009, respectively). The percentage of the men in group Ⅰ whose partners became pregnant was 29. 5% and those of groups Ⅱ and Ⅲ were 50% and 42.9%, respectively. Although the percentage among three groups was not different statistically (x2 = 3. 285, 0. 854, 0. 199, P= 0. 070, 0. 355, 0. 655, respectively), the relative risks of groups Ⅱ and Ⅲ were 2.4 and 1.8 times of that of group Ⅰ. There were 25, 15, 7 cases of idiopathic infertility among the 3 groups, respectively. The relative risk of I group was 1.5 and 1.3 times of that of Ⅱ and Ⅲ groups. The correlation analysis showed that the T/E2 in serum had significantly negative correlation with the percent of the sperm head or neck or tail defects (r= -0. 209, -0. 316 and -0. 335,respectively and P= 0. 048, 0. 002 and 0. 001, respectively). Conclusions The decrease of T/E2 in serum was correlative with the decrease of fertility probability, but it did not alter the sperm density and the sperm motility. It showed that the level of the T/E2 in serum was important for spermatogenesis and sperm fertilizing capability.

Objective To establish a method for simultaneously measuring L-citrulline and L-arginine concentration in plasma using reversed-phase high performance liquid chromatography (RP-HPLC)with ultraviolet detection,and the novel method is applied to the patients with digestive system tumor.Methods Plasma samples were deproteinized by trichloroacetic acid with heating method.Phenylisothiocyanate (PITC)was used as derivatization reagent and a gradient elution was adopted. The feasibility verification of method was carried out by detecting plasma L-citrulline and L-arginine acid concentration in 21 cases of advanced digestive tract malignant tumor patients,21 cases of non malignant tumor patients and 39 cases of healthy people.Results The linearity for L-citrulline and L-arginine ranged from 0 to at least 1 000 μmol/L.r=0.999 95 for both. The lower limits of quantification for L-citrulline and L-arginine were 0.240 μmol/L and 0.448 μmol/L respectively.The in-traday and interday coefficients of variation (CVs)were less than 3.4% and 7.2%,respectively.The average recovery rate was from 95.2% to 104.1%.The ratio of plasma L-citrulline/L-arginine in patients with digestive cancar was significantly higher than that in the healthy control group,t-test,P =0.009.The sensitivity and specificity of plasma L-citrulline/L-argi-nine ratio in the malignant tumor of digestive system were 81% and 71.4% respectively.Conclusion This method can pro-vide a reliable and efficient method for the clinical determination of the L-citrulline and L-arginine concentration in plasma. The ratio of plasma citrulline/arginine increased obviously in patients with digestive cancer,suggesting that this ratio is a more sensitive index than single concentration of L-citrulline or L-arginine when evaluating patients with digestive tumor.