Objective To observe the effects of puerarin on the expression of glycogen synthase kinase- 3 (GSK- 3) in the skeletal muscle of rats with insulin resistance induced by high fat and sucrose diets. Methods 30 Wistar rats were randomly divided into three groups: normal group, model group and puerarin group, 10 rats in each group. The model group and puerarin group were fed with the high fat and sucrose diets for 4 weeks. Then puerarin group was treated with puerarin by abdominal injection (100 mg/kg) for 6 weeks. At the end of the experiment, GSK- 3 content was detected by Western blot analysis. Body weight, serum triglyceride and cholesterol levels, fasting plasma glucose and serum insulin concentrations were measured regularly and insulin sensitivity index was also computed as well. Results The outcome of Western blot analysis showed that the expression of GSK- 3 in the skeletal muscle of the model group increased by 70.20 % (P

Objective To analyze the impact of age on the detection rate,grade and stage of prostate cancer in patients with prostate specific antigen (PSA) 4-10 μg/L.Methods A retrospective analysis was made in 426 patients with PSA 4-10 μg/L who underwent prostate biopsy and divided into 4 group according to their age less than 60 (n=33),60-69 (n=163),70-79 (n=202) and more than 80 (n=28) to compare the correlation between age and the cancer detective rate.Among them,115 patients,diagnosed as prostate cancer,were further divided into 4 group according to their age less than 60 (n =3),60-69 (n=32),70-79 (n=69) and more than 80 (n=11).The correlation between age and the Gleason score or clinical stage was analyzed in 115 prostate cancer patients.Results The detective rate in each group was 9.1％ (3/33),19.6％ (32/163),34.2％ (69/202) and 39.3％ (11/28).There was significant difference in prostate cancer detective rate among those groups.The cancer detective rate became significantly higher as the age increased (P＜0.001).In the patients with prostate cancer,the number of patients with Gleason score more than 7 in each group was 1 (50.0％),11 (37.9％),30 (44.8％),7 (63.6％).No significant correlation was found between age and grade (P=0.292 4).And each group had cases with localized prostate cancer was 2 (100.0％),14 (60.9％),39 (78.0％),4 (66.7％),with locally advanced prostate cancer 0,5 (21.7％),7 (14.0％),1 (16.7％),with metastatic prostate cancer 0,4 (17.4％),4(8.0％),1 (16.7％).No significant difference was found between each group and clinical stage (P =0.101 6,P=0.285 2,P=0.349 1).Conclusions The prostate cancer detective rate significantly increases with the age increasing.There is no significant correlation between age and grade or clinical stage in the patients with PSA 4-10 μg/L.

Objective To observe the effect of Tiaowei Xiaozhi Pills on gastric juice secretion and gastrointestinal function in rats model of damp stagnation syndrome. Methods The damp stagnation syndrome model was established in rats. The gastric juice,pepsin and gastrointestinal function were determined. Results Tiaowei Xiaozhi Pills in the dosages of 700 mg/kg and 350mg/kg can diminish the amount of gastric juice and pH level and increase pepsin level. Tiaowei Xiaozhi Pills in the dosages of 700 mg/kg,350 mg/kg,175 mg/kg can also improve gastrointestinal function in model rats. Conclusion Tiaowei Xiaozhi Pills have improvement on gastrointestinal function in rats model of damp stagnation syndrome.

AIM: To explore the effect of physcion (P) on the level of IL-1? and expression of ICAM-1 and caspase-3 during cerebral ischemia-reperfusion injury. METHODS: The 91 healthy adult SD rats were selected, and were randomly divided into normal group, sham-operated group, cerebral ischemia-reperfusion group (model), low-dose physcion (PLD) and high-dose physcion (PHD) treatment group. The level of IL-1? was detected by radioimmunoassay. The expression of ICAM-1 and caspase-3 was detected by immunohistochemistry. The changes of tissue pathology were also investigated. RESULTS: The level of IL-1? reached the peak at 6 h after ischemia-reperfusion (IR). The protein expression of ICAM-1 and caspase-3 reached the peak at 24 h after IR. The level of IL-1? and the protein expression of ICAM-1 and caspase-3 in PHD group decreased obviously compared with those in model group (P

ObjectiveTo understand the behavioral characteristics,strength on suicide ideation and mental disorder diagnosis among suicide attempers,so as to provide consultation for crisis intervention of suicide.Methods232 suicide attempters from emergency of 5 general hospital in south-west of Shandong province were enrolled.They were assessed using questionnaires on attempted suicide behaviors and scale on the strength of suicide ideation,and were diagnosed using the Structured Clinical Interview for DSM-Ⅳ Disorders axis Ⅰ for patient (SCID/I-P).Results( 1 ) In the total of 232 cases,female was more than male with the gender ratio of 2.87 (female vs male).The highest proportion rate (56.9％) existed between 16 years old to 35 years old.Oral application pesticide was the most commom suicide pattern (83.2％),91.4％ of the suicide attempt occurred in the their homes.The top three reasons leading to suicide were family contrary (61.2％),depressive mood (15.1％)and fail in love (9.5％).(2) In all cases,83.6％ did not think if they were found,84.1％ did not adopted any precaution measures,over 60.0％ did not tell anyone their attempted plans,and 90％ had no dying structions and /or dying testaments before their attempted behaviors.At the moment of attemp behaviors,62.9％ did not contact with someone.The 60.0％ of all the cases had wanted strongly to die,otherwise 7.3％ had wanted to manipulate others.(3) The diagnosis rate of mental disorder on DSM-Ⅳ was 45.3％,in which the mood disorder was most.ConclusionThere are no changes on the behavioral characteristics among the suicide attempters in the south-west rural area of Shandong province.Their strength of suicide ideation are moderate,and a certain proportion of suicide attempters may be impulsive suicide attempters.There is more relationship between mental disorder and suicide attempt.

Objective To determine the effects of trimetazidine (TMZ) on pathology and energy metabolism of myocardium in chronic renal failure(CRF) rats.Methods CRF models were built in Sprague-Dawley (SD) rats with 5/6 subtotal nephrectomy, and animals were randomyly divided into sham group, control group and three groups treated with different doses of TMZ (3 mg/kg,6 mg/kg or 9 mg/kg).TMZ was intragastrically administrated to CRF rats for 17 weeks, while physiologicalsalinewasusedascontrol. Transthoracicechocardiographywasperformedand myocardial morphosis was observed.Left ventricular weight/body weight(LVW/BW) and heart weight/body weight (HW/BW) were measured, and heart rate, and mean arterial pressure (MAP)were detected at the end of the study, while several parameters were detected, including urea nitrogen (BUN), creatinine(Scr), triphosaden(ATP), adenosine diphosphate(ADP), superoxide dismutase (SOD), malondialdehyde (MDA), interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α).Results (l)Left ventricle end-systolic dimensions, anterior wall end-diastolic and end-systolic thicknesses, and posterior wall end-diastolic thickness were significantly lower in rats treated with either medium dose or high dose of TMZ, as compared with control group(P＜0.05).(2)LVW/BW and HW/BW in rats treated with either medium dose or high dose of TMZ were significantly lower than those in control group(P＜0.05). (3)Various pathological changes were observed in control group, such as irregular arrangement and hypertrophy of the cardiomyocytes, myocardial fibrosis,mitochondrial swelling, focal muscle fiber dissolution, etc.However, all these pathological changes were apparently ameliorated in TMZ-treated groups, while the beneficial effects of TMZ therapy were dose-dependent. (4)No difference was observed in heart rate among all the groups.Although no difference existed in all the CRF rats, concerning on the systolic/diastolic blood pressure and mean arterial pressure (P＞0.05), these parameters were elevated in CRF rats, as compared with sham-operated group(P＜0.01). (5)ATP and ADP in TMZ-treated rats were significantly higher as compared with control(P＜0.05), moreover, medium dose and high dose of TMZ were superior to low dose (P＜0.05).(6)SOD was significantly increased in TMZ-treated rats (P＜0.05), while IL-6,TNF-α and MDA were significantly decreased in medium dose and high dose of TMZ, as compared with control(P＜0.05).Conclusion TMZ may prevent myocardial fibrosis and left ventricular hypertrophy in chronic renal failure via ameliorating myocardial energy metabolism and alleviating inflammatory reaction and oxidative stress.

Objective To explore the roles on psychosocial factors of suicide behavior in the suicide attempters.Methods132 suicide attempters and 132 matched controls treated in the emergency department were assessed using certain psychosocial questionnaires,such as Health Questionnaire of WHO,Questionnaire of Life Measure ( QLM),Beck Depression Inventory (BDI) and Beck Anxiety Inventory ( BAI),Barratt Impulsiveness Scale (BIS) and Buss Aggressiveness Scale ( BAS),Social Surpport Rate Scale and Questionnaire of Coping Style (QCS).Results( 1 ) The results of single-factor analysis showed that the scores of Health Questionnaire of WHO and QL were lower in the suicide attempter group than those in the control group( suicide attempter group:17.07±3.07 and 9.76 ±7.11; control group:13.42 ±3.31 and 17.08 ±3.81,all P＜0.05),otherwise the scores of BDI,BAI,BIS and BAS were higher( suicide attempter group:15.22 ± 11.22,27.87 ± 7.24,87.67 ±20.08 and 73.74 ± 19.61 ; control group:6.87 ± 6.36,26.22 ± 5.44,73.34 ± 14.54 and 63.78 ± 16.44,all P ＜ 0.05 ).Among QCS,the sub-score of self-blaming was higher( 3.42 ± 2.44 VS 2.35 ± 2.04,P ＜ 0.05 ),otherwise the sub-scores of question solvation,asking for help and rationalization were lower( all P ＜ 0.05 ).( 2 ) The result of multi-factors analysis showed that gender,mean education times,the scores of QL,HQ,BDI,and BIS,sub-score of rationalization,and so on,entried the Logistic aggressive equaltion.Conclusion The status of health,quality of life,depressive and anxious mood,impulsive and aggressive personality,and certain coping style play important role.

Objective To study biologic effect of high radon exposure in non-uranium miners by measurements of serum levels of 1L-2, IL-6 and TNF-u and study on their gene polymorphism. Methods Serum levels of IL-2, IL-6 and TNF-αin the miners selected from a Yunnan-based copper mine were measured by ABC-ELISA. TNF-α (-308,G→A) genotypes were identified by RFLP-PCR, IL-2 (-330, T→G) genotypes by sequence analysis. Results Compared the miners with its control group, there were no statistical significance of the concentrations of serum IL-2 (F=0.71, P>0.05), IL-6 (F=1.09, P>0.05) and TNF-α(F=0.95,P>0.05). Frequencies of IL-2 (-330, T→G) genotypes (X2=0.02, P>0.05) and TNF-α(-308, G→A) genotype (x2= 2.21, P>0.05) were shown no statistical significance too. Conclusions Compared with the control group, concentrations of serum IL-2, IL-6 and TNF-n in miners working in the copper mine was lower, frequencies of genotypes of TNF-o (-308,A/A) was higher in the miners. But all the differences were not statistical significant.

Objective To explore if the occupational exposure to low dose thorium could induce adaptive response in peripheral lymphocytes.Methods 40 individuals.who exposed to thorium and rare earth mixed dust(exposure group) or control in Baotou Steel Plant, were selected, and chromosome aberrations were analyzed.Then the peripheral blood samples were irradiated in vitro with 2 Gy60Co γ-rays,and unstable chromosome aberration or DNA stand breakage analysis using single cell gel electrophoresis was performed. Results The dicentrics before 2 Gy exposure in exposure group was higher than that in control(P>0.05). But the dicentries after 2 Gy exposure in exposure group was lower than that in control,but not significantly (P>0.05).The tricentrics in exposure group was significantly lower than that in control(U=3.1622, 0.0ol

Objective To study the relationship between lung ventilation function of workers exposed to rare earth dust and their IL-2,IL-6 and TNF-α gene polymorphism.Methods TNF-α gene polymorphism were identified by RFLP-PCR,IL-2 and IL-6 gene polymorphisms were identified by PCR-CTPP analysis.Lung ventilation function was deteced by instrument of ventilation function.Results Compared with controls,there was no statistic significance in frequencies distribution of TNF-α gene polymorphism(X2=4.03,P＞0.05),IL-2 gene polymorphism(X2=2.21,P＞0.05)and IL-6 gene polymorphism(X2=1.05,P＞0.05).Compared with IL-2 gene wild type,IL-2 homozygote type increased the risk of lung ventilation dysfunction by 4.29 folds(95% CI 1.09～16.9).Conclusions Compared with controls,incidence of ventilation function of workers exposed to rare earth dust is in ascending trend.IL-2(G/G)gene type induces more serious inflammation reaction than the others.