Objective To observe the effects of nerve impulses on the expression of carbonic anhydrase Ⅲ ( CAⅢ ) and its phosphatase activity, and to explore whether or not the cause of CAⅢ expressive decreased in skeletal muscles of myasthenia gravis( MG) is resulted from the obstruction of nerve impulse.Methods The motor nerves of extensor digitorum longus (EDL, mainly composed by fast fibers) and soleus (Sol, mainly composed by slow fibers) were cut off by operation of denervation.Levels and phosphatase activities of CAⅢ were analyzed at 7, 14, 28, and 56 d after denervation by Western blot and specific enzyme staining on the membrane following SDS-polyacrylamide gel electrophoresis, respectively.Results (1) Levels of CAⅢ in Sol of normal side (eg denervated contralateral) were much higher than that in EDL of normal side, and the levels in both Sol and EDL had an enhanced tendency with time (age) increase, especially for Sol.After denervation, the levels of CAⅢ in EDL were gradual increased, however, the level in Sol was 14 d after denervation as the boundary of ascension and then decline.( 2) The phosphatase activities of CAⅢ in Sol of normal sides were much higher than that in EDL of normal sides, and there were an enhanced tendency with time (age) increase in Sol, but no significant changes were found in EDL The enzyme activities in denervated Sol were lower(in the 14, 28, and 56 days after denervation: 14.39 ±1.93, 11.48 ±1.46, 9.04 ±1.46) much than their contralaterals(22.75 ± 1.80, 25.26 ±3.15, 25.82 ± 2.97; t = 0.002, 0.005, 0.002, all P ＜ 0.05), the enzyme activities in denervated EDL were also lower than their contralaterals, however, no significant differences were found.(3)It was consistent for CAⅢ levels and phosphatase activities in both Sol and EDL of normal sides.After denervation, however, the deviation of the CAⅢ levels and phosphatase activities happened, the levels of CAⅢ were increased, but the phosphatase activities were decreased.Conclusions The effect of nerve impulse transferring obstructed by denervation on CAⅢ expression of skeletal muscles is different from that by MG auto-antibody.The decrease of CAⅢ protein in the MG muscles may be not resulted from the nerve impulse transferring obstructed by MG auto-antibody.

BACKGROUND:Body mass is a critical factor affecting plantar pressure,and the systematic study on plantar pressure with different body mass has not been reported.OBJECTIVE:To measure the plantar pressure of normal people with different body weight,so as to provide reference for the application of plantar pressure.METHODS:Totally 80 healthy people with different body weight were enrolled,and the dynamic plantar pressure was measured by the Footscan 2 meter flat foot pressure distribution system (RSscan,Belgium).The participants were divided into four groups (n=20 per group) according to the classification criteria for prevention and control of overweight and obesity in Chinese adults,such as underweight,normal weight,overweight group,and obesity groups,and a comparative analysis was conducted.RESULTS AND CONCLUSION:(1) The peak pressure and impulse of each region of the foot were significantly increased with body mass index increasing (P ＜ 0.05).Compared with the normal weight people,the pressure of the part foot area of the overweight subjects and each part foot area of the obese subjects were significantly increased (P ＜ 0.05).(2) The percentage of the medial plantar contact area accounting the total contact area of the foot was significantly increased with body mass index increasing (P ＜ 0.01);the percentage in the obese group was significantly higher than that in the normal group (P ＜ 0.05).(3) These results show that the plantar pressure and impulse are increased in overweight and obese subjects due to weight gain,and foot will appear with pathological changes with time going.Obese people suffer from arch drop due to weight gain,and increase in contact area of midfoot,which are easy to cause flatfoot.Overweight especially obese,should pay attention to control weight,do appropriate exercise,wear suitable shoes,protect the foot,further to prevent the occurrence of foot injury and flatfoot.

Cardiac arrest in children is one of the most critical clinical situations,posing a serious threat to life.With the progress of cardiopulmonary resuscitation and the advanced life support technology,children's cardiac arrest survival rate is higher than before,but many of the survivors suffer from neurological sequelae.Since the causes of out-hospital cardiac arrest and in-hospital cardiac arrest are different,their prognosis and influence factors are also different.Death risk factors for cardiac arrest in children are cancer,hepatic insufficiency,acute renal failure,sepsis,systemic inflammatory response syndrome,etc.Cardiopulmonary resuscitation,as the main emergency treatment of cardiac arrest,is also one of the main factors affecting survival of cardiac arrest child.

The present study retrospectively analyzed 8 patients with hip dysplasia (8 hips) comprising 2 males and 6 females, aged 46 years old (range, 35-65 years old) who received treatment at the Department of Orthopedics, Second Affiliated Hospital of Nanchang University between September 2004 and November 2006. Preoperative radiographs and CT examination showed that all patients presented with sharp angle of affected hip > 45°, poor containment of femoral head, narrow joint space, and non-successive Shenton's line. Following Crowe classification, there were 5 cases (5 hips) of grade Ⅱ,and 3 cases (3 hips) of grade Ⅲ. Preoperative Harris hip score was 50 points (range, 44-62 points). Total hip arthroplasty was employed in each patient. Prosthesis was fixed by Cementless or cemented modes. Cementless fixation was applied in 6 hips and cemented fixation in 2 hips. Cementless prosthesis was used on the affected side in each patient. Replacement time, bleeding volume during surgery, hospital days post-surgery, Harris scores, and peri-operative complications were recorded.Simultaneously, post-surgery healing was observed. All 8 cases were followed up for an average period of 6 months. All patients exhibited well recovered joint function. No infection, sciatic nerve injury, dislocation of hip joint, fracture of femoral shaft, thrombosis of deep veins, or prosthesis loosening were found in any follow-up case. Six months after surgery, Harris hip score was 88 points (range, 86-94 points). Radiographs taken at 3 months post-surgery displayed the bone union of surgical region. All these findings suggest that acetabular medial wall displacement osteotomy in total hip arthroplasty produces obvious early- and middle-stage effects in treatment of hip dysplasia.

0.05). There were no adverse events or severe adverse events in all these 72 cases. Conclusion Ozone is effective and safe in the treatment of bacterial vaginosis.

45?,poor containment of femoral head,narrow joint space,and non-successive Shenton's line.Following Crowe classification,there were 5 cases(5 hips) of grade II,and 3 cases(3 hips) of grade III.Preoperative Harris hip score was 50 points(range,44-62 points).Total hip arthroplasty was employed in each patient.Prosthesis was fixed by Cementless or cemented modes.Cementless fixation was applied in 6 hips and cemented fixation in 2 hips.Cementless prosthesis was used on the affected side in each patient.Replacement time,bleeding volume during surgery,hospital days post-surgery,Harris scores,and peri-operative complications were recorded.Simultaneously,post-surgery healing was observed.All 8 cases were followed up for an average period of 6 months.All patients exhibited well recovered joint function.No infection,sciatic nerve injury,dislocation of hip joint,fracture of femoral shaft,thrombosis of deep veins,or prosthesis loosening were found in any follow-up case.Six months after surgery,Harris hip score was 88 points(range,86-94 points).Radiographs taken at 3 months post-surgery displayed the bone union of surgical region.All these findings suggest that acetabular medial wall displacement osteotomy in total hip arthroplasty produces obvious early-and middle-stage effects in treatment of hip dysplasia.

Objective To investigate the expressions of Survivin and high risk-human papillomavirus (HR-HPV) in cervical carcinoma and premalignant lesion,and explore their roles in the pathogenesis of cervical carcinoma.MethodsEighty-two patients of cervical intraepithelial neoplasia(CIN)and cervical carcinoma were enrolled,including 25 CIN Ⅰ,23 CIN Ⅱ -Ⅲ and 34 cervical carcinoma.Twenty normal cervicals were chosen as control.The expression of Survivin was examined by immunohistochemical assay(SP),and the infection of HR-HPV was measured by polymerase chain reaction (PCR).ResultsExpressions of Survivin and HR-HPV of patients with cervical carcinoma [ 85.3％(29/34)and 88.2％ (30/34) ]were higher than those in CIN [ 52.1％ (25/48) and 54.2％ (26/48) ](P ＜ 0.05 ),CIN were higher than those in normal cervicals [ 0 and 10.0％ ( 2/20 ) ](P ＜ 0.05 ),CIN Ⅱ - Ⅲ [ 65.2％ ( 15/23 ) and 73.9％ ( 17/23 ) ]were higher than those in CIN Ⅰ [ 32.0％ ( 8/25 ) and 28.0％ (7/25) ](P ＜ 0.05 ).Survivin positive expression in cervical carcinoma was correlation with histological grade,but was no correlation with (P ＜ 0.05),age,clinical stage and pathology typing (P ＞ 0.05 ).HR-HPV infection was no correlation with age,clinical stage,histological grade and pathology typing (P ＞ 0.05).Positive correlation between the Survivin positive expression and HR-HPV infection was observed in cervical carcinoma(r =0.403,P ＜0.05).ConclusionIt suggests that Survivin may play an important role in the occurrence and development of cervical carcinoma together with HR-HPV.

Objective:To investigate the effect of single-port laparoscopy and conventional laparoscopic surgery in ovarian cystectomy.Methods:The clinical data of 139 patients with laparoscopic surgery for ovarian cyst admitted to the same department of gynecology from December 2016 to December 2019 in Suzhou Hospital Affiliated of Anhui Medical University were retrospectively analyzed, and they were divided into single-port laparoscopic surgery group (62 cases) and conventional laparoscopic surgery group (77 cases) according to different surgical methods. The clinical indictors, the levels of estrogen, T lymphocyte subgroups, the complications rate and incision satisfaction rate between two groups were compared.Results:The operation in both groups was smooth and there was no conversion to open surgery. Compared with the conventional laparoscopic surgery group, the surgery time, intraoperative blood loss in single-port laparoscopic surgery group were higher, the hospital stays in single-port laparoscopic surgery group was shorter, but there were no significant differences ( P>0.05). The levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E 2) in two groups before operation had no significant differences ( P>0.05). At 30 d after operation, the levels of FSH, LH and E 2 in single-port laparoscopic surgery group were higher than those in conventional laparoscopic surgery group, but the differences were no statistically significant ( P>0.05). Before operation, the levels of CD 4+, CD 8+ and CD 4+/CD 8+ in two groups has no significant differences ( P>0.05). At 3 d after operation, the levels of CD 4+, CD 8+ and CD 4+/CD 8+ in single-port laparoscopic surgery group were higher than those in conventional laparoscopic surgery group: 0.51 ± 0.03 vs. 0.47 ± 0.02, 0.41 ± 0.23 vs. 0.39 ± 0.05, 1.36 ± 0.02 vs. 1.24 ± 0.02, and the differences were statistically significant ( P<0.05). The rate of complication in two groups had no significant difference ( P>0.05). At 3 d after operation, the scores of visual analogue scale (VAS) of pain in single-port laparoscopic surgery group were lower than that in conventional laparoscopic surgery group: (1.12 ± 0.21) scores vs. (3.56 ± 0.65) scores, and the difference was statistically significant ( P<0.05). The incision satisfaction rate in single-port laparoscopic surgery group was significantly higher than that in conventional laparoscopic surgery group: 90.32%(56/62) vs. 72.73%(56/77), and the difference was statistically significant ( P<0.05). Conclusions:Single-port laparoscopy can be safely and smoothly implemented in ovarian cystectomy, and can reduce postoperative pain, improve cell immunity, and have better cosmetic result.

Objective To analyze the anatomic factors of the central retinal artery occlusion or the ophthalmic artery occlusion after the injection of facial cosmetic surgery.Methods Retrospectively analyzed 3 patients who occurred severely ocular complications just after facial cosmetic injection in last 2 years.The diagnosis of central retinal artery occlusion was confirmed by fundus examination and fundus fluoresce-in angiography.Analysis the blood vessel distribution of the injection site and characteristics of peri-orbit vascular anastomosis.Results All the 3 cases presented no light perception,with eye pain or (and)the eyeball pain.The fundus test and fundus fluorescein angiographies showed central retinal artery obstruction.Facial cosmetic injection pressure significantly exceeded the ophthalmic artery systolic pressure 2 seconds after injection (P <0.05).Dorsal nasal artery and angular artery were anastomotic,and the angular artery was usually anastomotic with lateral nasal branches of the posterior ciliary artery.The filler can enter the ophthalmic artery by the branches of the anastomosis,which can make ophthalmic artery occlusion,central retinal artery occlusion or get occlusion of their branches.Conclusion The injectant may get into the orbital artery and retrograde to the ophthalmic artery,which resulting in ophthalmic artery occlusion,or get into central retinal artery and posterior ciliary artery,which resulting in serious ocular complications.