Laser tweezers Raman spectroscopy (LTRS) system is a combination of spectroscopy and laser tweezers; It is a new method of studying cells; It can trap single living cell and make Raman spectrum of single living cell. From the positions, intensities, and line widths of the Raman peaks in the spectra, we can get useful information about composition, structure and interactions of complexes inside the living cells. External agents may change cell's physiological state and this changed information can also be got from Raman spectra. This article is a study of Raman spectra of human red blood cell (RBC) affected by different intensity direct current (DC); from the result, distinct change of Raman spectra of RBC have been got. These changes characterize the changes of the internal information of the cells. This article give some academic reference of physical therapy using DC in the level of molecule.
Electricity ; Erythrocytes ; cytology ; physiology ; Humans ; Lasers ; Optical Tweezers ; Spectrum Analysis, Raman ; methods

Objective:To evaluate the characteristics of vaginal flora between normal and abnormal pregnant women throughout pregnancy.Methods:Vaginal swab specimens were collected from pregnant women in the first (<14 gestation weeks, GW), second (14~28 GW) and third trimester (>28 GW) in Anqing, Anhui Province from February 2018 to February 2020. Pregnant women were divided into normal and abnormal groups according to all clinical diagnosis. The sequences of 16S rRNA gene (V3-V4) from vaginal swabs were analyzed using QIIME2 platform. The differences in the dominance of Lactobacillus, community state type (CST) transition, Alpha diversity and Beta diversity were analyzed. Diversity data after log transition were used in the analysis of linear mixed model. Results:A total of 34 pregnant women (10 normal and 24 abnormal) with 102 samples were included for analysis. The composition of vaginal flora between two groups: the relative abundance of Lactobacillus was the highest at the genus level and Lactobacillus crispatus and Lactobacillus iners was the top two species with high relative abundance. The dominance of Lactobacillus, Alpha diversity and transition of CST were also similar. Both groups had a gradually decreased trend of Alpha diversity with GW, and the Chao1, Observed species and Faith′s PD indexes′ were different in different GW ( P<0.05). All Beta diversity metrics in normal group had descending trend, with lower value of the index of first distance which implied a higher microbiota stability, while Bray-Curtis, Weighted UniFrac distance had ascending trend in abnormal group, indicating lower stability. Jaccard distance′s first distance was statistically differed among GW and Unweighted UniFrac distance′s differed between normal and abnormal groups. Conclusions:The first distance of Unweighte UniFrac distance in abnormal pregnant women is higher than that of normal pregnant women and the vaginal flora in abnormal group has lower stability.