Objective To investigate the maternal-fetal transmission of human papillomavirus (HPV).Methods Samples of exfoliated cervical cells were obtained from 73 pregnant women on their third-trimester examinations. Samples of fetal membranes, amniotic fluid and nasopharyngeal swab were obtained from the parturients and their neonates. The presence of HPV types 16, 18 and 35 deoxyribonucleic acid was detected by polymerase chain reaction (PCR) and endonuclease method.Results HPV types 16, 18 and 35 deoxyribonucleic acid were found in 26 (35.6%) of 73 specimens of cervical cells obtained from the parturients, and in 31 (42.5%) of 73 specimens of fetal membranes, 6 (15.4%) of 39 specimens of amniotic fluid, and 5 (14.7%) of 34 neonatal nasopharyngeal swabs obtained from the neonates. The restriction endonuclease analysis of the amplified products showed that HPV types 16, 18 and 35 were positive in 24.7%, 9.6% and 1.4% respectively of parturients, and 11.0%, 4.1% and 0 respectively of neonates. The maternal-fetal transmission rate of HPV was 50% (7/14) for spontaneous vaginal delivery, and 33.3% (4/12) for cesarean section.Conclusion HPV can be transmitted from mothers to their babies not only through the placenta during pregnancy, but also through the genital tract during delivery.

Objective To investigate the effect of the limiting value of subfield number on the dosime-try of intensity-modulated radiotherapy (IMRT) plan for left-breast radical mastectomy in order to seek the optimal limiting value of subfield number.Methods The clinical data of 30 patients with left-breast radical mastectomy in this hospital from March 1,2022 to March 31,2023 were retrospectively analyzed.All patients used the Oncentra 4.3 planning system to design 5 kinds of IMRT plans,and the limiting values of subfield number were 15,25,35,45 and 55 respectively (all IMRT plans were named according to the subfield number limiting value,the other optimization parameters and objective function were the same),planning target vol-ume (PTV) dosimetric parameters,organ at risk (OAR) receiving dosage and monitor units were statistically analyzed and compared.Results The PTV D2％ (F=104.439,P<0.05),D98％ (F=20.748,P<0.05),Dmean (F=89.578,P<0.05),homogeneity index (HI,F=101.794,P<0.05) and conformity index (CI,F=26.453,P<0.05) among different subfields number limiting values of IMRT had statistical differences,the left side humeral head V50 (F=76.991,P<0.05) had significant difference and the other OAR had no signifi-cant difference (P>0.05).The PTV D2％,D98％,Dmean,CI and HI values of plan35 were significantly superior to those of plan15 and plan25,and the differences were statistically significant (P<0.05).Compared with plan35,there were no statistically significant difference in PTV D98％ and CI of plan45 and plan55 (P>0.05),while the PTV D2％,Dmean and HI were lower,and the difference was statistically significant (P<0.05).The V50 of left humeral head in plan15 and plan25 were too high to meet the clinical request.The plan35,plan45 and plan55 could protect the OAR well,moreover there were no significant difference in the receiving dosage of the OAR (P>0.05).The monitor units of plan35 was lower than that of plan45 and plan55,and the difference was statistically significant (P<0.05).Conclusion When the limiting value of subfield number is 35,the dose distribution of PTV and OAR receiving dosage meet the clinical dosimetric requirements,meanwhile the monitor units is lower,which can be used as a reference value for the IMPT plan design for left-breast cancer radical mastecto-my.

Objective:To investigate the association of osteosarcopenia with falls, risk of fracture, malnutrition among middle-aged and elderly adults.Methods:A total of 253 participants dwelling in Guangzhou community aged 40-90 years were included in this cross-sectional study from December 2017 to December 2019.Bone mineral density(BMD)was detected by dual-energy X-ray absorptiometry(DEXA). Body composition was analyzed by a bioelectrical impedance analysis.Handgrip strength and gait speed were examined.The 10-year probability of a major osteoporotic fracture and hip fracture were evaluated by online WHO fracture risk assessment tool( FRAX?). Results:According to diagnostic criteria of AWGS and EWGSOP2, the incidences of osteosarcopenia varied from 5.1% to 7.6%, 5.1% to 11.4% respectively.After the adjustment for age and gender, Logistic regression analysis showed that osteosarcopenia was correlated with falls, risk of fracture and malnutrition.Osteosarcopenia definited by AWGS criteria was strongly correlated with falls( OR=3.27-5.68, P<0.05), osteosarcopenia definited by non-severe sarcopenia criteria was strongly correlated with the risk of hip fracture( OR=1.14-1.15, P<0.05), and fat-free mass index was strongly correlated with osteosarcopenia with different definitions( OR=0.21-0.48, P<0.05). Conclusions:Osteosarcopenia is associated with higher risk of falls, fracture and malnutrition in the Guangzhou community-dwelling middle-aged and elderly adults, and fat-free mass index is an independent risk factor for osteosarcopenia.