The common rheumatic diseases in children including rheumatic fever,juvenile idiopathic arthritis,systemic lupus erythematosus (including neonatal lupus),juvenile dermatomyositis,Kawasaki disease,anaphylactoid purpura.Rheumatic diseases as a group of unknown etiology autoimmune diseases,connective tissue and collagen fibers of different organs can be affected.Systemic inflammatory is the common prominent characteristics of this kind of disease,often resulting in multiple organ damage.Over recent decades,there has been considerable interest in the long-term outcomes of individuals with chronical inflammatory disease and an area of particular concern has been the increased prevalence of cardiovascular disease.Since sustained systemic inflammation is known to accelerate atherosclerosis,doctor should pay attention to rheumatic diseases associated cardiovascular involvements during daily clinical work.The cardiovascular involvement of common rheumatic diseases in children is summarized in this paper.

Eighty percent of unexplained syncope in children is neurally mediated syncope (NMS).The cur-rent clinical diagnosis of children NMS mainly depends on the head -up tilt test(HUTT),which has a certain risk, therefore searching for a safe noninvasive NMS diagnosis method is of great significance.Twenty -four -hour ambulato-ry blood pressure monitoring (ABPM)is widely used in clinic recently.It was reported that there was autonomic nerve adjustment imbalance in children with NMS.And the diagnostic value of ambulatory blood pressure pattern to NMS chil-dren was high.Non -spoontype blood pressure variation pattern of NMS could aid to diagnose children NMS.Fur-therly study of 24 h ABPMof NMS children has great significance to explore the pathogenesis of the NMS,to be benefi-cial to explain the regulating mechanism of the vessels,and go a step further to guide the treatment.

Objective To set up a stable method to induce SW872 adipocyte to differentiation.Methods THE animals were randomly divided in to five groups according to different inducing agents and a control groups.The changes of the cells were observed by microscopy and Oil-Red O staining.TG mass of these cells were assayed by chemical colorimetry methods.Results The oleic acid is the strongest factor to induce SW872 cell to differentiate,IBMX+DEX+Insulin is also strong factor,but it took a longer time to act.The effect of IBMX is stronger than insulin and ASP,but less than Oleic acid and IBMX+DEX+Insulin.Oleic acid significantly increased triglyceride mass of SW872 cell on the 1~(th) day of differentiation,The TG mass in the oleic acid group increased on the 2~(th) and4~(th)day of differentiation. Conclusion Oleic acid is the best Inducer to stimulate SW872 cell to differentiate,it can induce SW872 preadipocytes to differentiate to adipicytes in a short term.

Objective To study the genetic polymorphisms of STR locus D20S85 and to obtain the genetic data of Guangdong Han, Guangxi Zhuang, Huadong Han,Neimenggu Han and Meng populations. Method Using PCR and silver - staining methods, the alleles of D20S85 locus were detected and analysed. Results 9 alleles and 35 genotypes were found in those populations. The highest allele frequency was D20S85* 6. The results of Hardy - Wein-berg equilibrium test showed that the genotype distributions observed in five populations were correspondent with the expected. The expected heterozygosity was 0.7720 - 0.7912; the exclusion probability of paternity was 0.7538-0.7594(triplet) and 0.3988 -0.4297 (biplet) ; the discrimination power 0.9175 -0.9272 and the polymorphism information content 0.7442 - 0.7656. Conclusion STR D20S85 locus is a useful genetic marker for forensic application .

Objective To investigate the polymorphism of DXS6854 locus in Guangdong Han population. Methods The DXS6854 locus was analyzed by PCR following polyacrylamide gel electrophoresis and silver staining. Results Among 189 females and 230 males from Guangdong Han population, 8 alleles were observed with frequencies ranging from 0.0026 to 0.4522. Exact tests demonstrated genotype frequencies in females had no departure from Hardy- Weinberg equilibrium. The discrimination powers for female and male original samples were 0.8633 and 0.7012, respectively. When both mother and her daughter were tested, the probability of excluding a random man as a father was 0.6712. Conclusion The DXS6854 locus is appropriate for individual identification and paternity testing involving a female child.

Objective To investigate the STR typing discordance between different typing methods.Methods Genotypes of 13 routine forensic STR loci in DNA samples from 100 individuals were typed by using singleplex polyacrylimide gel electrophoresis silver stain system and Power Plex16 System,respectively.The typing results between these two systems were compared.Results One genotype discordance was observed at D8S1179 locus in a DNA sample.The genotypes was 12/14 in snigleplex system and 12/15 in Power Plex16 System.Conclusion Different STR typing systems may result in different genotyps from the same DNA sample.

OBJECTIVETo detect plasma N-terminal pro-brain natriuretic peptide (NT-proBNP) in acute Kawasaki disease (KD) and analyze the relationship between NT-proBNP and other bio-markers in order to evaluate if NT-proBNP could be as a useful diagnostic marker to predict the risk of coronary arterial lesions in acute KD.

METHODTotally 106 patients with KD were recruited from January 2012 to April 2014 at Department of Pediatrics of Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology,64 were boys and 42 were girls, their age ranged from 2 months to 8 years and 4 months. Of the 106 cases, 48 had typical KD(TKD) and 58 incomplete KD(IKD). They were divided into two groups according to echocardiography results: coronary arterial lesions (KD-CAL, n = 33) and non coronary arterial lesions (KD-nCAL, n = 73). Forty children whose age and gender matched with respiratory tract infection were selected as control group, 22 were boys and 18 were girls, age range from 7 months to 7 years and 11 months. Plasma NT-proBNP levels were measured by using the enzyme-linked fluorescence analysis (ELFA) at the day of admission, meanwhile blood routine tests, liver function tests, determination of C-reactive protein (CEP), erythrocyte sedimentation rate (ESR), electrolytes were performed in these patients. Pearson's correlation analysis was used to evaluate the association. The ROC curve analysis was done to identify the threshold of coronary 'arterial lesions.

RESULTThe levels of NT-proBNP were (1 037 271) ng/L in TKD group and (1,325 ± 264) ng/L in IKD group. The levels of NT-proBNP in control group was (125 ± 22) ng/L. Both the levels of NT-proBNP in TKD and IKD group were significantly higher than that of control group (t = 3.360, 3.590; P < 0.05). The level of NT-proBNP in KD-CAL group was (2,775 ± 842) ng/L and that of KD-nCAL group was (830 ± 145) ng/L, NT-proBNP levels of KD-nCAL group was significantly higher than that of control group (t = 3.660, P = 0.007) ; moreover the level of NT-proBNP of KD-CAL group was also significantly higher than that of KD-nCAL group ( t = 3.860, P = 0.005). The levels of total white blood cell count, neutrophil percentage, platelet count, CRP and ESR of KD-CAL group were significantly higher than those of the control group, however there was no significant difference between KD-CAL group and KD-nCAL group. The levels of albumin and Na of KD-nCAL group were significantly lower than those of the control group. Plasma NT-proBNP level in KD group was positively correlated with white blood cell count, neutrophil percentage, and CRP (r = 0.239, P = 0.025; r = 0.359, P = 0.001; r = 0.474, P = 0.001), there was a negative correlation between albumin and Na (r = -0.303, P = 0.015; r = -0.338, P = 0.002). When the level of NT-proBNP was higher than 950 ng/L, the sensitivity for diagnosis of coronary arterial lesions in the KD was 88.1% and the specificity was 89.0%.

CONCLUSIONThe plasma NT-proBNP can be used as a useful parameter in early diagnosis of KD. Plasma NT-proBNP could be used to predict the risk of coronary arterial lesions in KD.

Biomarkers ; Blood Sedimentation ; C-Reactive Protein ; Case-Control Studies ; Child ; Child, Preschool ; Coronary Vessels ; pathology ; Female ; Humans ; Infant ; Male ; Mucocutaneous Lymph Node Syndrome ; complications ; Natriuretic Peptide, Brain ; blood ; Peptide Fragments ; blood ; Predictive Value of Tests ; ROC Curve ; Sensitivity and Specificity

The extended service in domestic and foreign medical libraries was summarized according to the trans-formation of medical library and the need of public health information followed by a description of some typical cases of extended service provided by MedlinePlus website , Disaster Medical Information Management Research Center , and patient libraries in order to provide the reference for domestic medical libraries to cary out their extended service .

Objective To evaluate the efficacy of entecavir treatment in chronic hepatitis B virus ( HBV ) infected patients with mild hepatic dysfunction and marked pathological injury.Methods One hundred and fifty five chronic hepatitis B ( CHB) patients with HBV DNA>1.0 ×104 U/mL admitted in Lishui People’ s Hospital during January 2008 to October 2011 were enrolled in the study.Patients were divided into three groups: those with serum ALT <2 ×ULN and liver inflammation injury ≥G2 and/or fibrosis stage≥S2 were in observation group ( n=75 ); patients with ALT ( 2-5 ) ×ULN were in control group 1 (n=38);patients with ALT>5 ×ULN were in control group 2 (n=42).All patients were given entecavir (0.5 mg, 1/d, p.o) treatment.ALT normalization rates, HBV DNA negative rates, HBeAg negative conversion rates and seroconversion rates at 12-, 24-,48-, 96-and 144-week were observed and compared among groups.Variance analysis andχ2 test were performed for measurement data and numeration data, respectively.Results ALT normalization rates in observation group were 86.7%, 90.7%, 90.7%, 92.0%and 96.0%at 12-, 24-, 48-, 96-and 144-week, which were higher than those in control group 1 (χ2 =2.04, 2.15, 2.78, 2.69 and 2.47, P <0.01), but no statistically significant difference was observed between observation group and control group 2 (χ2 =2.53,2.42,2.09,2.24 and 2.32,P>0.05) . HBV DNA negative rates in observation group were 70.7%, 78.7%and 82.7%at 12-, 24-and 48-week, which were higher than those in control group 1 (χ2 =4.56, 4.23 and 4.28, P<0.05), but no statistically significant difference was observed between observation group and control group 2 (χ2 =2.75, 2.62 and 2.98, P>0.05).HBeAg negative conversion rates in observation group were 6.6%, 21.3%and 25.3%at 48-, 96-, and 144-week, which were higher than those in control group 1 (χ2 =4.68, 4.78 and 5.01, P<0.05), but no statistically significant difference was observed between observation group and control group 2 (χ2 =2.24, 2.57 and 2.13, P>0.05).HBeAg seroconversion rate in observation group was 4.0%at 24-week, which were higher than that in control group 1 (χ2 =2.87, P <0.05), but the seroconversion rates at 96-and 144-week were lower than those in control group 2 (χ2 =2.92 and 3.14, P<0.05).Conclusion The efficacy of entecavir treatment for HBV infected patients with mild hepatic dysfunction and marked pathological injury is satisfactory.

Since the information supplied by the paternity testing of alleged parents was less than that of standard triplet parentage testing,so the paternity index (PI) calculating methods of standard triplet parentage testing was not suitable for calculating the PI value of alleged parents.In order to establish a more precise method for calculating PI value of alleged parents with STR typing results,the first thing is to summarize the standard triplet PI calculating formulas according to the Essen Mller theory.These formulas are 1/p,1/2p,1/p+q,1/2p+2q.This article reports a new PI calculating method in case of paternity testing of alleged parents.Compared with other methods,the new method for calculating Y value either considering random man and random female or considering the alleged father(mother)and random female(man).