1.Expression and significance of VEGF in endometriosis
Xuan QI ; Xiaoyan XIN ; Jiqing CHANG ; Detang WANG ; Huiling GUO ;
Journal of Medical Postgraduates 2003;0(03):-
Objectvies:To investigate the role of vascular endothelial growth factor(VEGF) in the pathogenesis of endometriosis(EMs). Methods:A total of 70 specimens of endometriosis and 30 specimens of the normal controls were evaluated by immunohistologically techique with polyclonal antibody against VEGF. Results:The expression levels of VEGF in endometriotic tissues was higher than that in the normal endometrium( P
2.Expression and significance of erythropoietin and its receptors in rats with traumatic brain injury
Qiang JIA ; Dashi ZHI ; Huiling HUANG ; Ying CAI ; Qiaoli WU ; Xuebin ZHANG ; Xiaoli CHANG
Chinese Journal of Trauma 2011;27(3):206-209
Objective To study the expressions of erythmpoietin(EPO)and its receptors(EPOR)in the injured brain tissue ofthe rats with traumatic brain injury(TBI).Methods A total of78 SD rats were randomly divided into three groups including control group(six rats),sham group(36rats) and fluid percussion injury group(36 rats).The rats were sacrificed at 6,24 hours,3,5,7 and 14days after TBI in the sham group and the fluid percussion injury group(six rats at each time point).Then,the injured brain tissues were removed for observation of the mRNA and protein expressions of EPO and EPOR by meaDiB of real-time PCR and Western blot. Results The expression of EPO was increased at 24 hours and reached the peak at day 3 after TBI.The hish expression level of EPO could maintain for two days or so.began to decrease at day 7 and recovered to normal at day 14 after Till.While the expression of EPOR reached the peak at 24 hours after TBI and maintained hish level at day14. Conclusions The expressions of EPO and EPOR show increase within 24 hours after TBI.In fact,the expressions of both factors are not in consistency,with more transient expression of EPO.
3.The analysing of ERPs P300 characeristics and the brain network connections in first-episode depressions
Cai NAN ; Gaohua WANG ; Xiaoping WANG ; Zhongchun LIU ; Huiling WANG ; Ling XIAO ; Chang SHU
Chinese Journal of Behavioral Medicine and Brain Science 2015;24(4):326-328
Objective To investigate the characteristics of the event related potential(ERP) P300 and analyze brain network connections in patients with first-episode depressions.Methods P300 auditory oddball task were administrated on twenty-nine patients and twenty-five healthy controls.The P300 amplitude and latency of two groups were compared,and the brain network connectivity of the two groups were analyzed using Granger's Causality analysis.Results The P300 amplitude in depression group were significantly different from those in control group (C3 of the central regions(15.77±7.35) μV vs (20.90±7.82)μV;C4 of the central regions(16.98±7.21) μV vs (22.11±7.50) μV;P3 of the parietal regions(15.65±6.92) μV vs (19.49±5.73) μV;P4 of the parietal(16.35± 6.46) μV vs P4(19.72±5.18) μV;P=0.009,P=0.007,P=0.017,P=0.024 respectively).However,the P300 latency had no significant difference comparing to the controls(P>0.05).The results also showed that patients had more connections in the brain network.Conclusion As an effective evaluation index,ERP P300 can play an important role in clinical diagnosis of depression.Patients suffering from depression have significant cognition function deficit.
4.Clinical efficacy of trimebutine combined with Xiangsha Pingwei in the treatment of functional dyspepsia and its effect on gastrointestinal hormone
Huiling YU ; Sucai LU ; Ying CHANG ; Ling YIN ; Yumei ZHANG ; Yaqing WANG ; Jing ZHANG ; Xiaoye WANG
Clinical Medicine of China 2017;33(8):690-694
Objective To investigate the clinical efficacy of trimebutine combined with Xiangsha Pingwei in the treatment of functional dyspepsia and its effect on gastrointestinal hormone.Methods One hundred and twenty cases of functional dyspepsia were randomly divided into the control group and observation group,60 cases in each group.The control group was treated with trimebutine 0.2 g,3 times/d and the observation group was treated with trimebutine 0.2 g,3 times/d and Xiangsha Pingwei 10 g,2 times/d.Both groups were treated for four weeks,a course of treatment.Clinical efficacy and the influence on gastrointestinal hormone levels were compared between the two groups.Results The symptoms scores in both groups all decreased significantly after treatment.While the postprandial fullness scores,early satiety scores and epigastric pain scores in the observation group were significantly lower than those in the control group (0.62±0.12 vs.1.85±0.25,0.56±0.14 vs.1.46±0.21,0.48±0.15 vs.1.32±0.12,t=34.36,27.62,33.87,P<0.05).The total effective rate in the observation group was significantly higher than that in the control group (90.00% vs.73.33%,χ2=5.57,P<0.05).MOT and GAS levels in both groups increased significantly.While MOT and GAS levels in the observation group were significantly higher than those in the control group (MOT: (355.2±78.1) ng/L vs.(288.7±82.4) ng/L;GAS:(96.5±17.6) ng/L vs.(88.4±16.0) ng/L,t=4.54,2.64,P<0.05).The somatostatin (SS) level in both groups decreased significantly.While the SS level in the observation group was significantly lower than that in the control group ((41.1±11.1) ng/L vs.(48.9±12.9) ng/L,t=3.55,P<0.05).Conclusion Trimebutine combined with Xiangsha Pingwei can significantly improve the clinical symptoms and gastrointestinal hormone in patients with functional dyspepsia.
5.Effects of mangiferin on tissue factor expressionin vascular endothelial cells and the underlying mechanisms
Yangyang WANG ; Huiling YU ; Yan CHEN ; Ying CHANG ; Yaning WEI ; Yingjian HOU
Chinese Pharmacological Bulletin 2017;33(7):961-965
Aim To explore the effects of mangiferin on tissue factor(TF) expression in human umbilical vein endothelial cells(HUVECs) and the underlying mechanisms.Methods HUVECs were isolated and primarily cultured in vitro.After the treatment with mangiferin and oxidized low density lipoprotein(oxLDL), TF expression was determined in HUVECs with real-time PCR and Western blot.Results oxLDLinduced the mRNA and protein expression and pro-thrombotic activity of TF in HUVECs.However, the inductive effects of oxLDL were blocked significantly by mangiferin.Furthermore, mangiferin modified TF expression and activity in a dose-dependent manner.Mangiferin was demonstrated to enhance the activity of peroxisome proliferator-activated receptor gamma(PPARγ).In contrast, GW9662, an antagonist of PPARγ, reversed at least partially the suppressive effects of mangiferin on TF.Conclusion Through activating PPARγ, mangiferin suppresses the expression of TF serving pro-thrombotic functions in endothelial cells.
6.Roles of glutamate transporter EAAT2 in occurrence and treatment of depression
Jianxin CHEN ; Lihua YAO ; Huiling WANG ; Zhongchun LIU ; Xiaoping WANG ; Ling XIAO ; Chang SHU ; Gaohua WANG
Chinese Pharmacological Bulletin 2016;32(7):894-897
The glutamate transporter EAAT 2 ( rodent nomencla-ture GLT-1:glutamate transporter 1), which is a predominantly astroglial glutamate transporter in the hippocampus and the pre-frontal cortex , is responsible for the majority of extracellular glu-tamate uptake .The glutamate transporter EAAT 2 can decrease the high levels of glutamate in the synaptic cleft , avoiding gluta-matergic excitotoxicity to damage the glial cells and neurons . Currently, the transporter EAAT2 has become a research hotspot of depression .This article aims to summarize roles of glutamate transporter EAAT2 in the occurrence and treatment of depres-sion.
7.Application of PET/CT radiomics in the treatment of non-small-cell lung cancer
Huiling LIU ; Qingtao QIU ; Cheng CHANG ; Yong YIN ; Ruozheng WANG
Chinese Journal of Radiological Medicine and Protection 2022;42(12):1015-1020
Primary lung cancer is the first malignant tumor in our country and in the world with an increasing mortality trend, which seriously endangers the human health. By digging the deep relationship between high-dimensional imaging features and pathophysiological features, radiomics can establish a predictive model to distinguish pathological types, tumor stages, distant metastases and survival, guide individualized diagnosis and treatment strategies, and improve prognosis. PET/CT has higher diagnostic accuracy and specificity by reflecting tumor tissue metabolism. This article reviews the application of PET/CT radiomics in the treatment of non-small-cell lung cancer (NSCLC).
8.Retrospective analysis of 185 inpatients with acute urticaria
Yuying KANG ; Xiao HE ; Yong LIU ; Huiling CHANG ; Qi DENG ; Yuanwen YANG ; Kai XU ; Rong GUO ; Li WANG
Chinese Journal of Dermatology 2018;51(3):224-227
Objective To analyze the precipitating factors for,clinical manifestations of,laboratory findings in and therapeutic effect on acute urticaria.Methods Clinical data were collected from 185 inpatients with acute urticaria in Shanxi Dayi Hospital from January 2013 to December 2016.Clinical features,laboratory examination results,treatment,prognosis and adverse reactions were analyzed retrospectively.Statistical analysis was carried out by chi-square test.Results There were 63 male patients and 122 female patients in this study,with an average age at onset of 32.87 ± 14.18 years.Of the 185 patients,78 (42.2%) were able to report the aetiological agents accurately,33 (17.8%) were induced by infection or drug therapy following infection,and 82 (44.3%) had fever.Blood cell analysis showed increased white blood cell count in 132 (71.4%) cases and increased proportion of neutrophils in 128 (69.2%) cases.The level of C reactive protein increased in 118 (69%) of 171 cases.A total of 185 patients received routine anti-anaphylactic treatment.Of 183 cured patients,153 (83.6%) were treated with antibiotics,26(14.2%)with antibiotics alone,and 24(13.1%) with azithromycin.There were 127 (69.4%) patients receiving combined treatment with glucocorticoids,antibiotics,and so on,and the antibiotic used in 111 (60.7%) cases was azithromycin.Of 88 cured patients with simultaneous signs of infection,85 (96.6%) showed increased levels of part or all of infection markers (including the white blood cell count,proportion of neutrophils and level of C reactive protein),and 69 (78.4%) were treated with azithromycin.Of 95 cured patients without signs of infection,83 (87.4%) showed increased levels of infection markers,and 61 (64.2%) were treated with azithromycin.Moreover,there were significant differences in the proportion of patients with increased levels of infection markers and that of patients treated with azithromycin between the cured patients with and without signs of infection (x2 =5.164,4.476,both P < 0.05).Conclusions Infection is a common cause of acute urticaria,and laboratory examinations including white blood cell count,proportion of neutrophils and level of C reactive protein are of important reference value to the diagnosis of infection in patients with acute urticaria.Patients with signs of infection or increased levels of infection markers need to be treated with combined anti-infective therapy,and in the cured patients,the proportion of patients administrating azithromycin was higher than that of those administrating other antibiotics for the treatment of acute infectious urticaria.
9.Application effect of 0.45% saline for airway humidification in patients with craniocerebral injury after tracheotomy
Yaohui PENG ; Huiling WANG ; Ying CHANG ; Juan LIN ; Ling ZHANG
Journal of Clinical Medicine in Practice 2017;21(18):57-59
Objective To investigate the effect of 0.45 % saline for airway humidification in patients with craniocerebral injury after tracheotomy.Methods A total of 58 patients with craniocerebral injury after tracheotomy treated in our department were as observation group,given 0.45% saline for airway humidification.And 40 craniocerebral injury patients after tracheotomy at the same time were as control group,given sterilized saline for airway humidification.The effect of airway humidification,the rate of sputum scab formation,and the incidence of adverse reactions were compared between the two groups.Results The pH of sputum after 3,5 d of tracheotomy in the observation group was lower (P < 0.05),but the effect of airway humidification and the rate of sputum scab formation in the two groups showed no statistically significant difference (P > 0.05).The incidence of pulmonary infection,irritating cough and respiratory mucosal bleeding in the observation group were lower than that in the control group (P < 0.05).Conclusion The 0.45% saline solution for airway humidification is in accordance with the physiological needs of the patients,and has better wetting effect and lower incidence of adverse events.
10.Application effect of 0.45% saline for airway humidification in patients with craniocerebral injury after tracheotomy
Yaohui PENG ; Huiling WANG ; Ying CHANG ; Juan LIN ; Ling ZHANG
Journal of Clinical Medicine in Practice 2017;21(18):57-59
Objective To investigate the effect of 0.45 % saline for airway humidification in patients with craniocerebral injury after tracheotomy.Methods A total of 58 patients with craniocerebral injury after tracheotomy treated in our department were as observation group,given 0.45% saline for airway humidification.And 40 craniocerebral injury patients after tracheotomy at the same time were as control group,given sterilized saline for airway humidification.The effect of airway humidification,the rate of sputum scab formation,and the incidence of adverse reactions were compared between the two groups.Results The pH of sputum after 3,5 d of tracheotomy in the observation group was lower (P < 0.05),but the effect of airway humidification and the rate of sputum scab formation in the two groups showed no statistically significant difference (P > 0.05).The incidence of pulmonary infection,irritating cough and respiratory mucosal bleeding in the observation group were lower than that in the control group (P < 0.05).Conclusion The 0.45% saline solution for airway humidification is in accordance with the physiological needs of the patients,and has better wetting effect and lower incidence of adverse events.